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The map label for this gene is cca [H]

Identifier: 220931559

GI number: 220931559

Start: 773500

End: 776097

Strand: Direct

Name: cca [H]

Synonym: Hore_07150

Alternate gene names: 220931559

Gene position: 773500-776097 (Clockwise)

Preceding gene: 220931558

Following gene: 220931560

Centisome position: 30.0

GC content: 37.61

Gene sequence:

>2598_bases
TTGGATATAATTATAACCCATGAGCTTACAGACCTGGATGGTCTGGGGGCAATGGTTGCTGCCAGAAAGCTGTACCCTCA
GGCTAGACCAGTTTTCCCCGGGAGATTACACAGAATGGTCAGGGACTTTATGGCCCTTTATAAAGATGAAATAAAAATAT
ATCAGGCCAGGGATATTGATTTTTCCAGGGTTACCAGGGTTATTTTAGTTGATACCCATAACCTTGATAAAATTGGCTCC
CTCAGGAACAGGTTAGACTGGGATAGGGTAGAAGTAATTTTGTATGATCATCATCCCCATGAATGCCTGAACTGGGTTAA
CAGGGATAAAAGCAGTAAAGTTGGTTCGGCCACAACAATTCTGGTTGAAGAAATTATTGAAAATGGAATAAGTTTAAATC
CACTTGAGGCTACTATTTGTGCCCTGGCTATTTATGCTGATACGGGAAAGCTTACATATCGGAATACAACTTCAAGTGAT
GCCAGGGCCCTGGCTTTTTTACTTGATAATGGTGCTAATTTAAAAGTAATAAATGAATTTCTAGAGGAATCCCTGACTGA
ACAGCAACAGGAGGTAATGGATTTACTTATAGAAAACAAAAAAGATATCGATATAAATGGGATAAAAATTACCCTATTCT
GGTTGGAATGTGATAACTATGTTATGGGACTAAACAGGATTGTTTCAAAAATTAAAGCTGTTTATCGGCTGCCCTCATTA
TTTATTATTGTTAAAATGGGTAATAAAGTTGAGGTTGTCGGTCGCAGCAGTGACGAGGTTGTAAATGTCGGCCAGATTTG
TGAATCCCTGGGTGGAGGTGGTCATCAGGGGGCAGGGGCAGTTCAGCTGCACACATCCCTTGAAAAGGCAAAAAGGGAGG
TTATCCGGGCGATAAAAGAAACTGTAAGACCTCCAGGAAAGGTCAGGGATATAATGTCCAGTCCTGTTAGAACAGTTAAC
CCTGATACCAGGATTGGAGAAGTTGAAAAAATACTTGATAAATATGGACATACCGGTATTGTTGTTTGTGAAAATGGGGA
GATTGTTGGAGTTTTTTCAAAACGTGACCTGAATAAGGTTAAAGAACATGATTTATTACATAGTCCTGTAAAGGGTTATA
TGTCCACTGATGTTATAACTATTGATGTTGATGAAACTATTCGTTATGCTCAGAAGTTAATGGTTAAATATGATATTGGA
AGGTTACCGGTTATAGAAGATGGTAAATTAGTGGGGATTGTGACCCGGAGTGATATTCTGTCGTCATACTATGGTATGAA
AACCCCCCACCAACACCTCAATAGATATGGCAGTACTCTGGTTAAAATAAAAGAAGAAAAATATGATTTCAGGCGAGTAT
TAAAAAGGTTGCCTGGAGAAGTATATTCACTATTAAATGTTATCAGGAGAGAGGCCGATAAACATGATGTCAGGGTATAC
CTTGTTGGGGGGATGGTTAGAGATCTAATTCTTGGTTTAGAAAATAATGATCTGGACTTTATGGTTGAAGGAAATATAAA
GTATTATTTAAGAGCTCTCTCCAGGACATTCGAGGAAAATTATACTTATAATGATAAATTTAAAACAGGCAGGATAAGAC
TCAACAGCGGGTATAATCTTGACTTTGCAGTGGCCAGGAGTGAGGTTTATACCAGTCCCGGAGCCCTTCCAGAAGTGGAA
ACAGGAGAAATTGTTGAGGATCTGTTCCGGCGGGACTTTACAGTGAATGCCCTTGCCATTGGTGTTGATAGTCAAAACTG
GGGGACATTGATTGATTTCTTCGGGGGAATACATGATATTGAACACAAAAAATTACGGGTGCTCCATCGATTCAGTTTCC
TGGATGATCCTACCAGAATTATAAGGGGGCTAAGACTGGCTTTGAGGCTCGGTTTTAGCTTTCAGGATAAGACAAATTTA
TTAATGAAGGAAGCACTCAAATTTGGTAATTTTAAGAGAATATCGAAGCCCAGAATTATTAAAGAATTTAGCCTTCTGTT
TAGTAAAAAAATGGATAGGAGGATAACAGGATTACTTAAACAGTTTCCGATATTTCACTTACTGGATTTAGATCCCCGGG
TTGATGAAAGCAAGTTTTCTATAATTAAGCGTGCCGAATGTATTCTTGATTATTTCAGACAAAAGAATTATAATATAAAA
GAATGGTTGATACGAATGGTAGTCCTGCTTGATGGAGTTGAAGAAAAAAAACTGGCAAACTGGGGATTGAACCGTGGTGA
AAGGGAAATTTTAACCTTTCCTTCTGTTATTGATACTATTATAGATAAATTGAGAACTAATTCTACTCCCTTAAAACTCA
AGAAACTTCTTGAGGGTTTCACTCTGGAACAGATATTACTCTTGTTGATATATACTGAAGGTAAAGAAAGGAAACAGGTA
TACAAATATATTAATTATCTCTCAAAGGTTGATTTAAAAATAAATGGACATGATTTAATTGAACTTGGTTTAAAACCCGG
ACCTGTCATTAAGGAAGTTCTAGATAAGGTCTGGGAGGCTAAAGTGGATAATGGCTTAAAAACAAGAGAGGAAGAACTTA
GATTTGCCAGAAAAATTATTGGTGATAAACTAAAGTGA

Upstream 100 bases:

>100_bases
GACCACCGGTTTTTACCGGTGGTTTTTTTATGTTTTTTATGAATAAATATACATAATCGGGGATTTAAAGTATGGTATAA
TAATGGTGGAGGCGATCAGA

Downstream 100 bases:

>100_bases
GGTAAAACAGATTATGAATTTAGAAGAAATTGTATTGTTAATCCCGGTTATTTTGCTATCTTTATCTATCCATGAATATT
CGCATGGCAGGGTATCATAC

Product: CBS domain containing protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 865; Mature: 865

Protein sequence:

>865_residues
MDIIITHELTDLDGLGAMVAARKLYPQARPVFPGRLHRMVRDFMALYKDEIKIYQARDIDFSRVTRVILVDTHNLDKIGS
LRNRLDWDRVEVILYDHHPHECLNWVNRDKSSKVGSATTILVEEIIENGISLNPLEATICALAIYADTGKLTYRNTTSSD
ARALAFLLDNGANLKVINEFLEESLTEQQQEVMDLLIENKKDIDINGIKITLFWLECDNYVMGLNRIVSKIKAVYRLPSL
FIIVKMGNKVEVVGRSSDEVVNVGQICESLGGGGHQGAGAVQLHTSLEKAKREVIRAIKETVRPPGKVRDIMSSPVRTVN
PDTRIGEVEKILDKYGHTGIVVCENGEIVGVFSKRDLNKVKEHDLLHSPVKGYMSTDVITIDVDETIRYAQKLMVKYDIG
RLPVIEDGKLVGIVTRSDILSSYYGMKTPHQHLNRYGSTLVKIKEEKYDFRRVLKRLPGEVYSLLNVIRREADKHDVRVY
LVGGMVRDLILGLENNDLDFMVEGNIKYYLRALSRTFEENYTYNDKFKTGRIRLNSGYNLDFAVARSEVYTSPGALPEVE
TGEIVEDLFRRDFTVNALAIGVDSQNWGTLIDFFGGIHDIEHKKLRVLHRFSFLDDPTRIIRGLRLALRLGFSFQDKTNL
LMKEALKFGNFKRISKPRIIKEFSLLFSKKMDRRITGLLKQFPIFHLLDLDPRVDESKFSIIKRAECILDYFRQKNYNIK
EWLIRMVVLLDGVEEKKLANWGLNRGEREILTFPSVIDTIIDKLRTNSTPLKLKKLLEGFTLEQILLLLIYTEGKERKQV
YKYINYLSKVDLKINGHDLIELGLKPGPVIKEVLDKVWEAKVDNGLKTREEELRFARKIIGDKLK

Sequences:

>Translated_865_residues
MDIIITHELTDLDGLGAMVAARKLYPQARPVFPGRLHRMVRDFMALYKDEIKIYQARDIDFSRVTRVILVDTHNLDKIGS
LRNRLDWDRVEVILYDHHPHECLNWVNRDKSSKVGSATTILVEEIIENGISLNPLEATICALAIYADTGKLTYRNTTSSD
ARALAFLLDNGANLKVINEFLEESLTEQQQEVMDLLIENKKDIDINGIKITLFWLECDNYVMGLNRIVSKIKAVYRLPSL
FIIVKMGNKVEVVGRSSDEVVNVGQICESLGGGGHQGAGAVQLHTSLEKAKREVIRAIKETVRPPGKVRDIMSSPVRTVN
PDTRIGEVEKILDKYGHTGIVVCENGEIVGVFSKRDLNKVKEHDLLHSPVKGYMSTDVITIDVDETIRYAQKLMVKYDIG
RLPVIEDGKLVGIVTRSDILSSYYGMKTPHQHLNRYGSTLVKIKEEKYDFRRVLKRLPGEVYSLLNVIRREADKHDVRVY
LVGGMVRDLILGLENNDLDFMVEGNIKYYLRALSRTFEENYTYNDKFKTGRIRLNSGYNLDFAVARSEVYTSPGALPEVE
TGEIVEDLFRRDFTVNALAIGVDSQNWGTLIDFFGGIHDIEHKKLRVLHRFSFLDDPTRIIRGLRLALRLGFSFQDKTNL
LMKEALKFGNFKRISKPRIIKEFSLLFSKKMDRRITGLLKQFPIFHLLDLDPRVDESKFSIIKRAECILDYFRQKNYNIK
EWLIRMVVLLDGVEEKKLANWGLNRGEREILTFPSVIDTIIDKLRTNSTPLKLKKLLEGFTLEQILLLLIYTEGKERKQV
YKYINYLSKVDLKINGHDLIELGLKPGPVIKEVLDKVWEAKVDNGLKTREEELRFARKIIGDKLK
>Mature_865_residues
MDIIITHELTDLDGLGAMVAARKLYPQARPVFPGRLHRMVRDFMALYKDEIKIYQARDIDFSRVTRVILVDTHNLDKIGS
LRNRLDWDRVEVILYDHHPHECLNWVNRDKSSKVGSATTILVEEIIENGISLNPLEATICALAIYADTGKLTYRNTTSSD
ARALAFLLDNGANLKVINEFLEESLTEQQQEVMDLLIENKKDIDINGIKITLFWLECDNYVMGLNRIVSKIKAVYRLPSL
FIIVKMGNKVEVVGRSSDEVVNVGQICESLGGGGHQGAGAVQLHTSLEKAKREVIRAIKETVRPPGKVRDIMSSPVRTVN
PDTRIGEVEKILDKYGHTGIVVCENGEIVGVFSKRDLNKVKEHDLLHSPVKGYMSTDVITIDVDETIRYAQKLMVKYDIG
RLPVIEDGKLVGIVTRSDILSSYYGMKTPHQHLNRYGSTLVKIKEEKYDFRRVLKRLPGEVYSLLNVIRREADKHDVRVY
LVGGMVRDLILGLENNDLDFMVEGNIKYYLRALSRTFEENYTYNDKFKTGRIRLNSGYNLDFAVARSEVYTSPGALPEVE
TGEIVEDLFRRDFTVNALAIGVDSQNWGTLIDFFGGIHDIEHKKLRVLHRFSFLDDPTRIIRGLRLALRLGFSFQDKTNL
LMKEALKFGNFKRISKPRIIKEFSLLFSKKMDRRITGLLKQFPIFHLLDLDPRVDESKFSIIKRAECILDYFRQKNYNIK
EWLIRMVVLLDGVEEKKLANWGLNRGEREILTFPSVIDTIIDKLRTNSTPLKLKKLLEGFTLEQILLLLIYTEGKERKQV
YKYINYLSKVDLKINGHDLIELGLKPGPVIKEVLDKVWEAKVDNGLKTREEELRFARKIIGDKLK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=196, Percent_Identity=31.6326530612245, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 99257; Mature: 99257

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIIITHELTDLDGLGAMVAARKLYPQARPVFPGRLHRMVRDFMALYKDEIKIYQARDID
CCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCC
FSRVTRVILVDTHNLDKIGSLRNRLDWDRVEVILYDHHPHECLNWVNRDKSSKVGSATTI
HHHHEEEEEEECCCCHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHCCCCCCCCCCHHHH
LVEEIIENGISLNPLEATICALAIYADTGKLTYRNTTSSDARALAFLLDNGANLKVINEF
HHHHHHHCCCCCCHHHHHHHHHEEEECCCCEEECCCCCCHHHEEEHHHCCCCCHHHHHHH
LEESLTEQQQEVMDLLIENKKDIDINGIKITLFWLECDNYVMGLNRIVSKIKAVYRLPSL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCE
FIIVKMGNKVEVVGRSSDEVVNVGQICESLGGGGHQGAGAVQLHTSLEKAKREVIRAIKE
EEEEEECCEEEEEECCCCCEECHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHH
TVRPPGKVRDIMSSPVRTVNPDTRIGEVEKILDKYGHTGIVVCENGEIVGVFSKRDLNKV
HCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEECCCHHHHH
KEHDLLHSPVKGYMSTDVITIDVDETIRYAQKLMVKYDIGRLPVIEDGKLVGIVTRSDIL
HHHHHHHCCHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEEEHHHHH
SSYYGMKTPHQHLNRYGSTLVKIKEEKYDFRRVLKRLPGEVYSLLNVIRREADKHDVRVY
HHHCCCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEE
LVGGMVRDLILGLENNDLDFMVEGNIKYYLRALSRTFEENYTYNDKFKTGRIRLNSGYNL
EEHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCE
DFAVARSEVYTSPGALPEVETGEIVEDLFRRDFTVNALAIGVDSQNWGTLIDFFGGIHDI
EEEEEHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHCCHHHC
EHKKLRVLHRFSFLDDPTRIIRGLRLALRLGFSFQDKTNLLMKEALKFGNFKRISKPRII
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHH
KEFSLLFSKKMDRRITGLLKQFPIFHLLDLDPRVDESKFSIIKRAECILDYFRQKNYNIK
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
EWLIRMVVLLDGVEEKKLANWGLNRGEREILTFPSVIDTIIDKLRTNSTPLKLKKLLEGF
HHHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHCCCCCHHHHHHHHCCC
TLEQILLLLIYTEGKERKQVYKYINYLSKVDLKINGHDLIELGLKPGPVIKEVLDKVWEA
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCEEEECCCCCHHHHHHHHHHHHH
KVDNGLKTREEELRFARKIIGDKLK
HHCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDIIITHELTDLDGLGAMVAARKLYPQARPVFPGRLHRMVRDFMALYKDEIKIYQARDID
CCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCC
FSRVTRVILVDTHNLDKIGSLRNRLDWDRVEVILYDHHPHECLNWVNRDKSSKVGSATTI
HHHHEEEEEEECCCCHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHCCCCCCCCCCHHHH
LVEEIIENGISLNPLEATICALAIYADTGKLTYRNTTSSDARALAFLLDNGANLKVINEF
HHHHHHHCCCCCCHHHHHHHHHEEEECCCCEEECCCCCCHHHEEEHHHCCCCCHHHHHHH
LEESLTEQQQEVMDLLIENKKDIDINGIKITLFWLECDNYVMGLNRIVSKIKAVYRLPSL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCE
FIIVKMGNKVEVVGRSSDEVVNVGQICESLGGGGHQGAGAVQLHTSLEKAKREVIRAIKE
EEEEEECCEEEEEECCCCCEECHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHH
TVRPPGKVRDIMSSPVRTVNPDTRIGEVEKILDKYGHTGIVVCENGEIVGVFSKRDLNKV
HCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEECCCHHHHH
KEHDLLHSPVKGYMSTDVITIDVDETIRYAQKLMVKYDIGRLPVIEDGKLVGIVTRSDIL
HHHHHHHCCHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEEEHHHHH
SSYYGMKTPHQHLNRYGSTLVKIKEEKYDFRRVLKRLPGEVYSLLNVIRREADKHDVRVY
HHHCCCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEE
LVGGMVRDLILGLENNDLDFMVEGNIKYYLRALSRTFEENYTYNDKFKTGRIRLNSGYNL
EEHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCE
DFAVARSEVYTSPGALPEVETGEIVEDLFRRDFTVNALAIGVDSQNWGTLIDFFGGIHDI
EEEEEHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHCCHHHC
EHKKLRVLHRFSFLDDPTRIIRGLRLALRLGFSFQDKTNLLMKEALKFGNFKRISKPRII
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHH
KEFSLLFSKKMDRRITGLLKQFPIFHLLDLDPRVDESKFSIIKRAECILDYFRQKNYNIK
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
EWLIRMVVLLDGVEEKKLANWGLNRGEREILTFPSVIDTIIDKLRTNSTPLKLKKLLEGF
HHHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHCCCCCHHHHHHHHCCC
TLEQILLLLIYTEGKERKQVYKYINYLSKVDLKINGHDLIELGLKPGPVIKEVLDKVWEA
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCEEEECCCCCHHHHHHHHHHHHH
KVDNGLKTREEELRFARKIIGDKLK
HHCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA