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The map label for this gene is punA [H]

Identifier: 220931540

GI number: 220931540

Start: 755363

End: 756175

Strand: Direct

Name: punA [H]

Synonym: Hore_06960

Alternate gene names: 220931540

Gene position: 755363-756175 (Clockwise)

Preceding gene: 220931539

Following gene: 220931541

Centisome position: 29.3

GC content: 40.34

Gene sequence:

>813_bases
ATGTTAAATAAAATTAAAGAAGCTTCCAGTTTTATTGAGGAAAAAATAAACATAAAACCTGACCTTGGATTGATTTTGGG
ATCTGGTCTCGGGGTTTTAGCTGAAGAGATTGAAAACCCCATTGTTATCCCTTATAAGGATATTCCCAATTTTCCGTTAT
CTACAGTAGAAGGCCATGCGGGTCAGCTTGTTATCGGTGAACTTGAAGGTAATAATGTTATTACTATGCAGGGGCGTTTT
CACCATTATGAAGGGTATTCACTGCAGGAATTAACAATGCCAGTCCGGGTTATGGGACAACTGGGGGTTAACAAATTAAT
TGTTACCAATGCAGCAGGTGGTATTAACCTAAATTTCAGGCCAGGTGATTTAATGCTGATAACCGATCATATAAATATGA
TGGGAGATAATCCCCTGATCGGTAAAAACTTTGATGAGTTTGGTCCCCGTTTTCCGGATATGACCTATGCCTACAGTAAA
GACTTGATCGAAATTGCTGAAAGGGTAGCTTCTTCCCGGGGGATTAATGTGAGGAAAGGTGTGTATGTAGCAGTAACAGG
TCCCAGTTTTGAGACACCCGCAGAAATAAGGTTTTTACGGAGGATTGGGGCTGATGCCGTTGGGATGTCTACCGTTCCTG
AGGTAATAGTTGCCAATCACATGGGTTTTGATGTTCTGGGTATTTCCTGTATAAGTAATATGGCTGCTGGTGTTTTACCG
GAGCCTCTCTCTCATGATGATGTTATGGAGATTTCAAGTAAGGTTAAACCTAAATTTATAAACCTGGTTAGAGGGATATT
AGAAGAAATGTAG

Upstream 100 bases:

>100_bases
TCAGGTTTTAATTTATTTTTTAGTAACAAAACTAATATATTTTTATGAAATTTTTGTTTAAATCGTTTAATTAGACAAAA
ATGAGAGGAGGAATTTTTAG

Downstream 100 bases:

>100_bases
GGGGGGTATCATGAGAGCATATGATATCATTTATAAAAAAAGGGAAGGTTTTAAATTATCAAAAGAGGAAATAGATTTTT
TAATTCAGGAATATACCCGT

Product: purine nucleoside phosphorylase I, inosine and guanosine-specific

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MLNKIKEASSFIEEKINIKPDLGLILGSGLGVLAEEIENPIVIPYKDIPNFPLSTVEGHAGQLVIGELEGNNVITMQGRF
HHYEGYSLQELTMPVRVMGQLGVNKLIVTNAAGGINLNFRPGDLMLITDHINMMGDNPLIGKNFDEFGPRFPDMTYAYSK
DLIEIAERVASSRGINVRKGVYVAVTGPSFETPAEIRFLRRIGADAVGMSTVPEVIVANHMGFDVLGISCISNMAAGVLP
EPLSHDDVMEISSKVKPKFINLVRGILEEM

Sequences:

>Translated_270_residues
MLNKIKEASSFIEEKINIKPDLGLILGSGLGVLAEEIENPIVIPYKDIPNFPLSTVEGHAGQLVIGELEGNNVITMQGRF
HHYEGYSLQELTMPVRVMGQLGVNKLIVTNAAGGINLNFRPGDLMLITDHINMMGDNPLIGKNFDEFGPRFPDMTYAYSK
DLIEIAERVASSRGINVRKGVYVAVTGPSFETPAEIRFLRRIGADAVGMSTVPEVIVANHMGFDVLGISCISNMAAGVLP
EPLSHDDVMEISSKVKPKFINLVRGILEEM
>Mature_270_residues
MLNKIKEASSFIEEKINIKPDLGLILGSGLGVLAEEIENPIVIPYKDIPNFPLSTVEGHAGQLVIGELEGNNVITMQGRF
HHYEGYSLQELTMPVRVMGQLGVNKLIVTNAAGGINLNFRPGDLMLITDHINMMGDNPLIGKNFDEFGPRFPDMTYAYSK
DLIEIAERVASSRGINVRKGVYVAVTGPSFETPAEIRFLRRIGADAVGMSTVPEVIVANHMGFDVLGISCISNMAAGVLP
EPLSHDDVMEISSKVKPKFINLVRGILEEM

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=266, Percent_Identity=46.9924812030075, Blast_Score=228, Evalue=4e-60,
Organism=Escherichia coli, GI1788746, Length=256, Percent_Identity=43.75, Blast_Score=219, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17541190, Length=259, Percent_Identity=44.015444015444, Blast_Score=209, Evalue=9e-55,
Organism=Saccharomyces cerevisiae, GI6323238, Length=298, Percent_Identity=40.6040268456376, Blast_Score=197, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24656093, Length=275, Percent_Identity=48, Blast_Score=251, Evalue=3e-67,
Organism=Drosophila melanogaster, GI45552885, Length=275, Percent_Identity=48, Blast_Score=251, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24656090, Length=275, Percent_Identity=48, Blast_Score=251, Evalue=3e-67,
Organism=Drosophila melanogaster, GI45552887, Length=275, Percent_Identity=48, Blast_Score=251, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24762376, Length=240, Percent_Identity=39.5833333333333, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29477; Mature: 29477

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNKIKEASSFIEEKINIKPDLGLILGSGLGVLAEEIENPIVIPYKDIPNFPLSTVEGHA
CCCHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCC
GQLVIGELEGNNVITMQGRFHHYEGYSLQELTMPVRVMGQLGVNKLIVTNAAGGINLNFR
CEEEEEEECCCEEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEC
PGDLMLITDHINMMGDNPLIGKNFDEFGPRFPDMTYAYSKDLIEIAERVASSRGINVRKG
CCCEEEEEECCHHCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECC
VYVAVTGPSFETPAEIRFLRRIGADAVGMSTVPEVIVANHMGFDVLGISCISNMAAGVLP
EEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
EPLSHDDVMEISSKVKPKFINLVRGILEEM
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MLNKIKEASSFIEEKINIKPDLGLILGSGLGVLAEEIENPIVIPYKDIPNFPLSTVEGHA
CCCHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCC
GQLVIGELEGNNVITMQGRFHHYEGYSLQELTMPVRVMGQLGVNKLIVTNAAGGINLNFR
CEEEEEEECCCEEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEC
PGDLMLITDHINMMGDNPLIGKNFDEFGPRFPDMTYAYSKDLIEIAERVASSRGINVRKG
CCCEEEEEECCHHCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECC
VYVAVTGPSFETPAEIRFLRRIGADAVGMSTVPEVIVANHMGFDVLGISCISNMAAGVLP
EEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
EPLSHDDVMEISSKVKPKFINLVRGILEEM
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537218; 8969508; 9384377; 1629150 [H]