Click here to switch to the map view.

The map label for this gene is mltD [C]

Identifier: 220931484

GI number: 220931484

Start: 691971

End: 694364

Strand: Direct

Name: mltD [C]

Synonym: Hore_06400

Alternate gene names: 220931484

Gene position: 691971-694364 (Clockwise)

Preceding gene: 220931482

Following gene: 220931485

Centisome position: 26.84

GC content: 38.18

Gene sequence:

>2394_bases
TTGAAGTATGAGAAGAAGGTCATACTTCCAGTACTAATATTATTAATACTTGTTATATTCCTGGCAGATGTGCAGGCTTT
GAATTATAAGCAGAGTTTCAGTAATTATACCATTTATACAGTAAGGCCTGGTGATAGTTTATATAAACTATCTAATAGGT
ATGGGGTATCTATATCTACGATAAAGATGCTTAACAACCTGAATTCCAGTATTATATATGTTGGACAAAGGTTAAAAATT
CCCGTAAATATGAAAAAATATAAAGTTAGACCCGGTGATACCCTTTATTTAATATCATTAAAATTTAATATTTCTGTTAA
GGATATAAAGGAAATTAATAATCTTACTTCGAATTTGATTTTTACTGGGCAAGAACTTTTAATTCCAGTGGAGGATACCG
GTAATTCCGATGATAGTTTGGACAATTATATGTTCTATACAGTTAAAGCTGGTGATAGTTTATATAAGATAGCAAAACAA
TTTGGAACCAGTATCCAGGTCCTGAAAGATATTAATAACTTCAATACTAATATTATTTATATTGGTCAAAAAATTAAGGT
GCCGCGAATTACTTATCAACTGGAAGTATTGACCAGAGGAGAGGGCAGGGTAATTAAGGAGATAGTCTCTGGAGATAAAA
AGGAAAATGGTTATACCCCTGATAGCCGTATCAGGCTCACGGCCCGGGGTAATAATGGAATGGTCTTTTCACACTGGACA
GGTGATGTTAGCAGTAGAGAAAATCCAGTTATAGTTAAAATGGATAGAGATAAGTCCGTTACAGCTGTTTTTTCAACTTC
TAAATTAAAAGAAGTCTTAATTAAAGGGAATGTTAATATCAGTAATAAAACAGGTGGCTTTAAATCGGTTAGAAAGGGTA
AAGACGCAGAACATTCCATTATACCCCTGCGGGTTTATAATGGATTTATGCCAGCTTACGAAGATTCAGAAATCATTGTA
AAGTATAAACCGGTAGTCAATGCCCAGTCTGTAGATAAACTGGAGAAAGTAAATGAGCTGGTTACCCTTAATGAAATGAA
TACCGGTGAAGGCCGGGTAGTTCGTTACAAGGTTCCCCGGGATAAAGATCTAACTGAATTCCTTGAATATTATGGCAATC
AGGATTTTGTTGAATGGGTTGAACCTAATTACATTTACTATCCAACTGGATTACCAGAAGACCCTTACTACCCTATATAT
CAATGGGATTTTGTCAACTTAAATTTAGAGGCAGCCTGGGATAAAGAAACAGGAAGTAATTCAACCATGGTTGCGGTTCT
TGATACCGGTATAGTACCAAATCACCCGGATTTAAAAGGTAATTTATTACAGGGAGCTGATTTTGTGGGAGGTAATAACT
CCCATCCTGTTTCTTCATTTCAGGTTACGGATTATGAACCTGTTGATGAGACTACCAGAGAAATGGGTGGTAGTCATGGG
ACCCATGTTGCCGGGGTTATTGGTGCTCTTACCAGTAATGGCAGGGGGATATCCGGTATCAACTGGAATGTAAAAATACT
ACCGGTCCGGGTTTTAAAGAAAACTGGTGGGACAAGCTGGGATATCGCTGAAGGTATTTATTATGCAATTGACCAGGGAG
CAAAGGTAATAAACTTAAGCCTGGGTAGCAAGTACGACAGCCGTCTTCAGGAAGAAGCTGTTGAGGTAGCCTATAAAAGA
GGTGTTACTGTGGTTGCAGCTGCCGGTAATGAAAATTCGCCTGTATATTATCCTGCAGCTTTCCCTGAAGTTATAGCCGT
TGCTGCTGTAAGTAAGGATAATACCAAGGCCTATTATTCTAATTATGGGCCCGAAATAGATGTCGCCGCCCCCGGTGGCG
GTTATGGTGAATCTATCTACAGTACCTGGGGTTACTATGAACAGGGAGAGACTGTACCGGGGTATGTTGGCATGATCGGA
ACTTCTATGGCAGCCCCCCATGTTAGTGGAATTGCTGCTTTATTGATATCAAGTGGAGTTGAGAATCCTGATGAGGTTCG
AAACCGTCTAATTAGTACAGCTATTGATCTGGGGGCTACAGGTAAAGATGATTATTACGGTTATGGTCTGGTTGATGCCT
ATGGAGCCCTGCTGGGGAAAAAACTTCAGGATCCGGTAGTGTTTACGGCAACAAAGGAAGGTAATTCTTTGATTGTAAAA
AGTGATATTAATAAGATTGATAAAGAGGGTAGCTTTTCTTTGAGGAGTAAACCGGGTCAAAATATGATTGTTGCCGCCTG
GCGTGATGTAAATAGCAATGGGGTTGTTGATAAAGGTGATTATTACGGTATGACCGGGCCTCTGGATATAACCGGTATTA
TTGAAAATATCAATGTTAAAATAAATTATATCTCTAAATCGACATTAAAAGATAATCTAACAGTTGTAAAATAA

Upstream 100 bases:

>100_bases
AAAAACCTTAATAAAAAATATTTTACATAACTTACTATTGTTACTATCATATTAAATATTGTATAATTAAAAAGACAAAA
TAGAGAAGGAGGGGTGTTTA

Downstream 100 bases:

>100_bases
ACATGGGCAAGGGGGTAGAATTTTGAAATCTTTATGTAATGGAAAAAGACTTATATTATTAATAATAATACTTGTGTTAG
TTACAAACCAACTGGTTCTG

Product: peptidase S8/S53 subtilisin kexin sedolisin

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Ak.1 protease [H]

Number of amino acids: Translated: 797; Mature: 797

Protein sequence:

>797_residues
MKYEKKVILPVLILLILVIFLADVQALNYKQSFSNYTIYTVRPGDSLYKLSNRYGVSISTIKMLNNLNSSIIYVGQRLKI
PVNMKKYKVRPGDTLYLISLKFNISVKDIKEINNLTSNLIFTGQELLIPVEDTGNSDDSLDNYMFYTVKAGDSLYKIAKQ
FGTSIQVLKDINNFNTNIIYIGQKIKVPRITYQLEVLTRGEGRVIKEIVSGDKKENGYTPDSRIRLTARGNNGMVFSHWT
GDVSSRENPVIVKMDRDKSVTAVFSTSKLKEVLIKGNVNISNKTGGFKSVRKGKDAEHSIIPLRVYNGFMPAYEDSEIIV
KYKPVVNAQSVDKLEKVNELVTLNEMNTGEGRVVRYKVPRDKDLTEFLEYYGNQDFVEWVEPNYIYYPTGLPEDPYYPIY
QWDFVNLNLEAAWDKETGSNSTMVAVLDTGIVPNHPDLKGNLLQGADFVGGNNSHPVSSFQVTDYEPVDETTREMGGSHG
THVAGVIGALTSNGRGISGINWNVKILPVRVLKKTGGTSWDIAEGIYYAIDQGAKVINLSLGSKYDSRLQEEAVEVAYKR
GVTVVAAAGNENSPVYYPAAFPEVIAVAAVSKDNTKAYYSNYGPEIDVAAPGGGYGESIYSTWGYYEQGETVPGYVGMIG
TSMAAPHVSGIAALLISSGVENPDEVRNRLISTAIDLGATGKDDYYGYGLVDAYGALLGKKLQDPVVFTATKEGNSLIVK
SDINKIDKEGSFSLRSKPGQNMIVAAWRDVNSNGVVDKGDYYGMTGPLDITGIIENINVKINYISKSTLKDNLTVVK

Sequences:

>Translated_797_residues
MKYEKKVILPVLILLILVIFLADVQALNYKQSFSNYTIYTVRPGDSLYKLSNRYGVSISTIKMLNNLNSSIIYVGQRLKI
PVNMKKYKVRPGDTLYLISLKFNISVKDIKEINNLTSNLIFTGQELLIPVEDTGNSDDSLDNYMFYTVKAGDSLYKIAKQ
FGTSIQVLKDINNFNTNIIYIGQKIKVPRITYQLEVLTRGEGRVIKEIVSGDKKENGYTPDSRIRLTARGNNGMVFSHWT
GDVSSRENPVIVKMDRDKSVTAVFSTSKLKEVLIKGNVNISNKTGGFKSVRKGKDAEHSIIPLRVYNGFMPAYEDSEIIV
KYKPVVNAQSVDKLEKVNELVTLNEMNTGEGRVVRYKVPRDKDLTEFLEYYGNQDFVEWVEPNYIYYPTGLPEDPYYPIY
QWDFVNLNLEAAWDKETGSNSTMVAVLDTGIVPNHPDLKGNLLQGADFVGGNNSHPVSSFQVTDYEPVDETTREMGGSHG
THVAGVIGALTSNGRGISGINWNVKILPVRVLKKTGGTSWDIAEGIYYAIDQGAKVINLSLGSKYDSRLQEEAVEVAYKR
GVTVVAAAGNENSPVYYPAAFPEVIAVAAVSKDNTKAYYSNYGPEIDVAAPGGGYGESIYSTWGYYEQGETVPGYVGMIG
TSMAAPHVSGIAALLISSGVENPDEVRNRLISTAIDLGATGKDDYYGYGLVDAYGALLGKKLQDPVVFTATKEGNSLIVK
SDINKIDKEGSFSLRSKPGQNMIVAAWRDVNSNGVVDKGDYYGMTGPLDITGIIENINVKINYISKSTLKDNLTVVK
>Mature_797_residues
MKYEKKVILPVLILLILVIFLADVQALNYKQSFSNYTIYTVRPGDSLYKLSNRYGVSISTIKMLNNLNSSIIYVGQRLKI
PVNMKKYKVRPGDTLYLISLKFNISVKDIKEINNLTSNLIFTGQELLIPVEDTGNSDDSLDNYMFYTVKAGDSLYKIAKQ
FGTSIQVLKDINNFNTNIIYIGQKIKVPRITYQLEVLTRGEGRVIKEIVSGDKKENGYTPDSRIRLTARGNNGMVFSHWT
GDVSSRENPVIVKMDRDKSVTAVFSTSKLKEVLIKGNVNISNKTGGFKSVRKGKDAEHSIIPLRVYNGFMPAYEDSEIIV
KYKPVVNAQSVDKLEKVNELVTLNEMNTGEGRVVRYKVPRDKDLTEFLEYYGNQDFVEWVEPNYIYYPTGLPEDPYYPIY
QWDFVNLNLEAAWDKETGSNSTMVAVLDTGIVPNHPDLKGNLLQGADFVGGNNSHPVSSFQVTDYEPVDETTREMGGSHG
THVAGVIGALTSNGRGISGINWNVKILPVRVLKKTGGTSWDIAEGIYYAIDQGAKVINLSLGSKYDSRLQEEAVEVAYKR
GVTVVAAAGNENSPVYYPAAFPEVIAVAAVSKDNTKAYYSNYGPEIDVAAPGGGYGESIYSTWGYYEQGETVPGYVGMIG
TSMAAPHVSGIAALLISSGVENPDEVRNRLISTAIDLGATGKDDYYGYGLVDAYGALLGKKLQDPVVFTATKEGNSLIVK
SDINKIDKEGSFSLRSKPGQNMIVAAWRDVNSNGVVDKGDYYGMTGPLDITGIIENINVKINYISKSTLKDNLTVVK

Specific function: Murein-Degrading Enzyme. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division (By Similarity). [C]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI27894285, Length=349, Percent_Identity=26.9340974212034, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI20336188, Length=349, Percent_Identity=26.9340974212034, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI20336190, Length=349, Percent_Identity=26.9340974212034, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI4505577, Length=349, Percent_Identity=26.9340974212034, Blast_Score=70, Evalue=1e-11,
Organism=Homo sapiens, GI20336180, Length=349, Percent_Identity=26.9340974212034, Blast_Score=70, Evalue=1e-11,
Organism=Homo sapiens, GI20336184, Length=349, Percent_Identity=26.9340974212034, Blast_Score=68, Evalue=3e-11,
Organism=Homo sapiens, GI20336182, Length=349, Percent_Identity=26.9340974212034, Blast_Score=68, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319893, Length=252, Percent_Identity=33.7301587301587, Blast_Score=98, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324576, Length=285, Percent_Identity=31.2280701754386, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320775, Length=247, Percent_Identity=30.3643724696356, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45550681, Length=268, Percent_Identity=27.9850746268657, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 87927; Mature: 87927

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00018 EF_HAND_1 ; PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYEKKVILPVLILLILVIFLADVQALNYKQSFSNYTIYTVRPGDSLYKLSNRYGVSIST
CCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCEEEEEECCCCHHHEECCCCCCCHHH
IKMLNNLNSSIIYVGQRLKIPVNMKKYKVRPGDTLYLISLKFNISVKDIKEINNLTSNLI
HHHHHCCCCEEEEECCEEEECCCCEEEEECCCCEEEEEEEEEECCHHHHHHHHHHHHCEE
FTGQELLIPVEDTGNSDDSLDNYMFYTVKAGDSLYKIAKQFGTSIQVLKDINNFNTNIIY
EECCEEEEEECCCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCEEEHHHHCCCCCEEEE
IGQKIKVPRITYQLEVLTRGEGRVIKEIVSGDKKENGYTPDSRIRLTARGNNGMVFSHWT
EECEEECCEEEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEEC
GDVSSRENPVIVKMDRDKSVTAVFSTSKLKEVLIKGNVNISNKTGGFKSVRKGKDAEHSI
CCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHEEECCCEECCCCCCHHHHHCCCCCCCCE
IPLRVYNGFMPAYEDSEIIVKYKPVVNAQSVDKLEKVNELVTLNEMNTGEGRVVRYKVPR
EEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHEEEEECCCCCCCEEEEEECCC
DKDLTEFLEYYGNQDFVEWVEPNYIYYPTGLPEDPYYPIYQWDFVNLNLEAAWDKETGSN
CCCHHHHHHHHCCCCEEHCCCCCEEEECCCCCCCCCCCEEEEEEEEEEEEEEECCCCCCC
STMVAVLDTGIVPNHPDLKGNLLQGADFVGGNNSHPVSSFQVTDYEPVDETTREMGGSHG
CEEEEEEECCCCCCCCCCCCCEECCCEECCCCCCCCCCEEEECCCCCCHHHHHHHCCCCC
THVAGVIGALTSNGRGISGINWNVKILPVRVLKKTGGTSWDIAEGIYYAIDQGAKVINLS
CCHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCHHCCEEEEECCCCEEEEEE
LGSKYDSRLQEEAVEVAYKRGVTVVAAAGNENSPVYYPAAFPEVIAVAAVSKDNTKAYYS
CCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCCCCEEEEEECCCCCEEEEC
NYGPEIDVAAPGGGYGESIYSTWGYYEQGETVPGYVGMIGTSMAAPHVSGIAALLISSGV
CCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCC
ENPDEVRNRLISTAIDLGATGKDDYYGYGLVDAYGALLGKKLQDPVVFTATKEGNSLIVK
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCEEEEE
SDINKIDKEGSFSLRSKPGQNMIVAAWRDVNSNGVVDKGDYYGMTGPLDITGIIENINVK
CCCHHCCCCCCEEECCCCCCCEEEEEEEECCCCCCEECCCCCCCCCCCEEEEEEEEEEEE
INYISKSTLKDNLTVVK
EEEEECCCCCCCCEEEC
>Mature Secondary Structure
MKYEKKVILPVLILLILVIFLADVQALNYKQSFSNYTIYTVRPGDSLYKLSNRYGVSIST
CCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCEEEEEECCCCHHHEECCCCCCCHHH
IKMLNNLNSSIIYVGQRLKIPVNMKKYKVRPGDTLYLISLKFNISVKDIKEINNLTSNLI
HHHHHCCCCEEEEECCEEEECCCCEEEEECCCCEEEEEEEEEECCHHHHHHHHHHHHCEE
FTGQELLIPVEDTGNSDDSLDNYMFYTVKAGDSLYKIAKQFGTSIQVLKDINNFNTNIIY
EECCEEEEEECCCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCEEEHHHHCCCCCEEEE
IGQKIKVPRITYQLEVLTRGEGRVIKEIVSGDKKENGYTPDSRIRLTARGNNGMVFSHWT
EECEEECCEEEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEEC
GDVSSRENPVIVKMDRDKSVTAVFSTSKLKEVLIKGNVNISNKTGGFKSVRKGKDAEHSI
CCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHEEECCCEECCCCCCHHHHHCCCCCCCCE
IPLRVYNGFMPAYEDSEIIVKYKPVVNAQSVDKLEKVNELVTLNEMNTGEGRVVRYKVPR
EEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHEEEEECCCCCCCEEEEEECCC
DKDLTEFLEYYGNQDFVEWVEPNYIYYPTGLPEDPYYPIYQWDFVNLNLEAAWDKETGSN
CCCHHHHHHHHCCCCEEHCCCCCEEEECCCCCCCCCCCEEEEEEEEEEEEEEECCCCCCC
STMVAVLDTGIVPNHPDLKGNLLQGADFVGGNNSHPVSSFQVTDYEPVDETTREMGGSHG
CEEEEEEECCCCCCCCCCCCCEECCCEECCCCCCCCCCEEEECCCCCCHHHHHHHCCCCC
THVAGVIGALTSNGRGISGINWNVKILPVRVLKKTGGTSWDIAEGIYYAIDQGAKVINLS
CCHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCHHCCEEEEECCCCEEEEEE
LGSKYDSRLQEEAVEVAYKRGVTVVAAAGNENSPVYYPAAFPEVIAVAAVSKDNTKAYYS
CCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCCCCEEEEEECCCCCEEEEC
NYGPEIDVAAPGGGYGESIYSTWGYYEQGETVPGYVGMIGTSMAAPHVSGIAALLISSGV
CCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCC
ENPDEVRNRLISTAIDLGATGKDDYYGYGLVDAYGALLGKKLQDPVVFTATKEGNSLIVK
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCEEEEE
SDINKIDKEGSFSLRSKPGQNMIVAAWRDVNSNGVVDKGDYYGMTGPLDITGIIENINVK
CCCHHCCCCCCEEECCCCCCCEEEEEEEECCCCCCEECCCCCCCCCCCEEEEEEEEEEEE
INYISKSTLKDNLTVVK
EEEEECCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7993087; 10588904 [H]