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The map label for this gene is lytF [H]

Identifier: 220931485

GI number: 220931485

Start: 694387

End: 696027

Strand: Direct

Name: lytF [H]

Synonym: Hore_06410

Alternate gene names: 220931485

Gene position: 694387-696027 (Clockwise)

Preceding gene: 220931484

Following gene: 220931486

Centisome position: 26.93

GC content: 36.75

Gene sequence:

>1641_bases
TTGAAATCTTTATGTAATGGAAAAAGACTTATATTATTAATAATAATACTTGTGTTAGTTACAAACCAACTGGTTCTGGC
CGGTACATTTGCACCGGCTTCATTAAATATTGCTGGAAAGGATGTAGTCGGGCTCAGTAGTTTTCAGACTGTAAGTGAAG
AGAGTGTAATTCCCCTAAATCTTTCATCTGATGCATTAAAAGCATTGTCCCATCAGAATTTTGAAATCGTTGGTTATACT
AATTTAGATACCTTGAAAAAAGATGATTATAGGTCAACGGTGGTTTATTATGTAAAGCCAGGGGATAGCCTGTATAAAAT
TGCCAGCAGATTTAATATTACCACACAGGAAATAAAAAATTTCAATAATTTAAAAACAAATGTCCTGTATGTTGGGCAAA
AATTATATATTCCGGTAGCTGAACAAAACACAGAGGTTTATTATGTAAGAGCAGGAGATAGTTTATATACAATTGCCGGG
AAATTTAATACCACCATTTCCCGGATAAAACAGGTTAATGGTTTGACTTCGGACATGCTTTATATAGGCCAGAAGTTATA
TATACCAACTGGTGATAAACAGACTGACCAGAAAGAACAGGTTTATTATGTAAAACCCGGGGATAGTTTATACAATATTG
CCAGAAGATTTAATACCACAGTACAGGAGTTAAAAGATTATAATGGCCTTAAGTCAAATATGCTTTATGTGGGACAGAAA
TTATACATCCCATCTCCTGATAAGTCCAGTGATAACAGGGGAGAAATGGGGAAGGTTATATATTTTGTGGAACCGGGAGA
CAGTCTCTACTCAATTGCCACGAAATTTAAGACAACAGTTGAAAGGATAAAGGAAATTAATGCTCTGGCTTCAGACAGTC
TTTATGTCGGGCAAAAGCTTTTTATTCCTTATCCAGAAGGTAGTAATACTGACCCCAAGGTTATTTATTTTGTGAAACCC
GGTGATACCCTTTATATAATCGCTATAAAATTCGGAACTACAGTAGAGCTGATAAAAGAAGTAAACAATTTAAGCAGTGA
AATTTTATATGTGGGTCAGAAGCTTTTAATTCCCGGTCCTCCTGAACTGGATGAAAAAGCTGATTTTGACCTCACCTATA
CTGTTGAACCCGGTGATAATATTTTCAGGATTGCCAGTAAATTTGGCATGGAAGTTAGTAAAATTATGTCATACAATGAC
CTGACATATGGTCATTTAAGGGAAGGGCAGCAAATTAAAATTCCCTTTTTCCTTCCTGATAGAGAACTTAACAATGGATT
TCAGGTAACTTCAGAGGAACTGGAACTCCTTGCCAGAGCAGTGTATTCAGAAGCCAGAGGTGAACCCTTTAAAGGTCAGG
TAGCGGTAGCTGCAGTTATTATCAATAGAATCAGCCATCCCTTTTTCCCCAATACGATAAGGGATGTAATTTTCCAGCCC
TGGCAGTTCAGTGCGGTTCATGATGGCCAGTTCTGGCTTGAACCCAACCAAACAGCATATATTGCAGCTAAAGCTGCTTT
AAGAGGTTGGGATCCAACTAAAGGAGCTATTTATTATTATAACCCTGATACTGCAACTTCTGACTGGGTTTTTTACCGGA
ATGTAGTTATTAAAATAGGTAATCATTATTTTGCTGTGTAG

Upstream 100 bases:

>100_bases
ATTATTGAAAATATCAATGTTAAAATAAATTATATCTCTAAATCGACATTAAAAGATAATCTAACAGTTGTAAAATAAAC
ATGGGCAAGGGGGTAGAATT

Downstream 100 bases:

>100_bases
CTGAAAAAGGAGTTGTATAGCTGAAAAAGGAGTTATAAAAAGCAGCCTTATGGGGCTGCTTTTATTTTTAATTAAATTCT
GTATATAACCAGGAACATTA

Product: cell wall hydrolase SleB

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Cell wall-associated polypeptide CWBP49; Gamma-D-glutamate-meso-diaminopimelate muropeptidase lytF [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MKSLCNGKRLILLIIILVLVTNQLVLAGTFAPASLNIAGKDVVGLSSFQTVSEESVIPLNLSSDALKALSHQNFEIVGYT
NLDTLKKDDYRSTVVYYVKPGDSLYKIASRFNITTQEIKNFNNLKTNVLYVGQKLYIPVAEQNTEVYYVRAGDSLYTIAG
KFNTTISRIKQVNGLTSDMLYIGQKLYIPTGDKQTDQKEQVYYVKPGDSLYNIARRFNTTVQELKDYNGLKSNMLYVGQK
LYIPSPDKSSDNRGEMGKVIYFVEPGDSLYSIATKFKTTVERIKEINALASDSLYVGQKLFIPYPEGSNTDPKVIYFVKP
GDTLYIIAIKFGTTVELIKEVNNLSSEILYVGQKLLIPGPPELDEKADFDLTYTVEPGDNIFRIASKFGMEVSKIMSYND
LTYGHLREGQQIKIPFFLPDRELNNGFQVTSEELELLARAVYSEARGEPFKGQVAVAAVIINRISHPFFPNTIRDVIFQP
WQFSAVHDGQFWLEPNQTAYIAAKAALRGWDPTKGAIYYYNPDTATSDWVFYRNVVIKIGNHYFAV

Sequences:

>Translated_546_residues
MKSLCNGKRLILLIIILVLVTNQLVLAGTFAPASLNIAGKDVVGLSSFQTVSEESVIPLNLSSDALKALSHQNFEIVGYT
NLDTLKKDDYRSTVVYYVKPGDSLYKIASRFNITTQEIKNFNNLKTNVLYVGQKLYIPVAEQNTEVYYVRAGDSLYTIAG
KFNTTISRIKQVNGLTSDMLYIGQKLYIPTGDKQTDQKEQVYYVKPGDSLYNIARRFNTTVQELKDYNGLKSNMLYVGQK
LYIPSPDKSSDNRGEMGKVIYFVEPGDSLYSIATKFKTTVERIKEINALASDSLYVGQKLFIPYPEGSNTDPKVIYFVKP
GDTLYIIAIKFGTTVELIKEVNNLSSEILYVGQKLLIPGPPELDEKADFDLTYTVEPGDNIFRIASKFGMEVSKIMSYND
LTYGHLREGQQIKIPFFLPDRELNNGFQVTSEELELLARAVYSEARGEPFKGQVAVAAVIINRISHPFFPNTIRDVIFQP
WQFSAVHDGQFWLEPNQTAYIAAKAALRGWDPTKGAIYYYNPDTATSDWVFYRNVVIKIGNHYFAV
>Mature_546_residues
MKSLCNGKRLILLIIILVLVTNQLVLAGTFAPASLNIAGKDVVGLSSFQTVSEESVIPLNLSSDALKALSHQNFEIVGYT
NLDTLKKDDYRSTVVYYVKPGDSLYKIASRFNITTQEIKNFNNLKTNVLYVGQKLYIPVAEQNTEVYYVRAGDSLYTIAG
KFNTTISRIKQVNGLTSDMLYIGQKLYIPTGDKQTDQKEQVYYVKPGDSLYNIARRFNTTVQELKDYNGLKSNMLYVGQK
LYIPSPDKSSDNRGEMGKVIYFVEPGDSLYSIATKFKTTVERIKEINALASDSLYVGQKLFIPYPEGSNTDPKVIYFVKP
GDTLYIIAIKFGTTVELIKEVNNLSSEILYVGQKLLIPGPPELDEKADFDLTYTVEPGDNIFRIASKFGMEVSKIMSYND
LTYGHLREGQQIKIPFFLPDRELNNGFQVTSEELELLARAVYSEARGEPFKGQVAVAAVIINRISHPFFPNTIRDVIFQP
WQFSAVHDGQFWLEPNQTAYIAAKAALRGWDPTKGAIYYYNPDTATSDWVFYRNVVIKIGNHYFAV

Specific function: Cleaves gamma-D-glutamate-meso-diaminopimelate bonds. Cell wall hydrolase involved in cell autolysis [H]

COG id: COG1388

COG function: function code M; FOG: LysM repeat

Gene ontology:

Cell location: Secreted, cell wall. Note=LysM repeats are thought to be involved in peptidoglycan binding [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 LysM repeats [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01476 LysM; PF00877 NLPC_P60 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 61534; Mature: 61534

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLCNGKRLILLIIILVLVTNQLVLAGTFAPASLNIAGKDVVGLSSFQTVSEESVIPLN
CCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCCEECCCHHCCCCCCCEEEEE
LSSDALKALSHQNFEIVGYTNLDTLKKDDYRSTVVYYVKPGDSLYKIASRFNITTQEIKN
CCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHC
FNNLKTNVLYVGQKLYIPVAEQNTEVYYVRAGDSLYTIAGKFNTTISRIKQVNGLTSDML
CCCCEEEEEEECCEEEEEEECCCCEEEEEEECCEEEEEECCHHHHHHHHHHHCCCHHHHH
YIGQKLYIPTGDKQTDQKEQVYYVKPGDSLYNIARRFNTTVQELKDYNGLKSNMLYVGQK
HHCCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCE
LYIPSPDKSSDNRGEMGKVIYFVEPGDSLYSIATKFKTTVERIKEINALASDSLYVGQKL
EEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEE
FIPYPEGSNTDPKVIYFVKPGDTLYIIAIKFGTTVELIKEVNNLSSEILYVGQKLLIPGP
EEECCCCCCCCCEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHEEECCEEECCCC
PELDEKADFDLTYTVEPGDNIFRIASKFGMEVSKIMSYNDLTYGHLREGQQIKIPFFLPD
CCCCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHCCCCCCHHHCCCCCEEEEEEEECC
RELNNGFQVTSEELELLARAVYSEARGEPFKGQVAVAAVIINRISHPFFPNTIRDVIFQP
CCCCCCCEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCC
WQFSAVHDGQFWLEPNQTAYIAAKAALRGWDPTKGAIYYYNPDTATSDWVFYRNVVIKIG
CEEEEEECCEEEECCCCEEEEEEEHHHCCCCCCCCEEEEECCCCCCCCEEEEEEEEEEEC
NHYFAV
CEEECC
>Mature Secondary Structure
MKSLCNGKRLILLIIILVLVTNQLVLAGTFAPASLNIAGKDVVGLSSFQTVSEESVIPLN
CCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCCEECCCHHCCCCCCCEEEEE
LSSDALKALSHQNFEIVGYTNLDTLKKDDYRSTVVYYVKPGDSLYKIASRFNITTQEIKN
CCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHC
FNNLKTNVLYVGQKLYIPVAEQNTEVYYVRAGDSLYTIAGKFNTTISRIKQVNGLTSDML
CCCCEEEEEEECCEEEEEEECCCCEEEEEEECCEEEEEECCHHHHHHHHHHHCCCHHHHH
YIGQKLYIPTGDKQTDQKEQVYYVKPGDSLYNIARRFNTTVQELKDYNGLKSNMLYVGQK
HHCCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCE
LYIPSPDKSSDNRGEMGKVIYFVEPGDSLYSIATKFKTTVERIKEINALASDSLYVGQKL
EEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEE
FIPYPEGSNTDPKVIYFVKPGDTLYIIAIKFGTTVELIKEVNNLSSEILYVGQKLLIPGP
EEECCCCCCCCCEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHEEECCEEECCCC
PELDEKADFDLTYTVEPGDNIFRIASKFGMEVSKIMSYNDLTYGHLREGQQIKIPFFLPD
CCCCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHCCCCCCHHHCCCCCEEEEEEEECC
RELNNGFQVTSEELELLARAVYSEARGEPFKGQVAVAAVIINRISHPFFPNTIRDVIFQP
CCCCCCCEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCC
WQFSAVHDGQFWLEPNQTAYIAAKAALRGWDPTKGAIYYYNPDTATSDWVFYRNVVIKIG
CEEEEEECCEEEECCCCEEEEEEEHHHCCCCCCCCEEEEECCCCCCCCEEEEEEEEEEEC
NHYFAV
CEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 10206711 [H]