Click here to switch to the map view.

The map label for this gene is gatA

Identifier: 220931081

GI number: 220931081

Start: 239083

End: 240516

Strand: Direct

Name: gatA

Synonym: Hore_02330

Alternate gene names: 220931081

Gene position: 239083-240516 (Clockwise)

Preceding gene: 220931080

Following gene: 220931082

Centisome position: 9.27

GC content: 42.96

Gene sequence:

>1434_bases
ATGGAATTATATGATCTTACAATCCATGAGTTGCGGGATCTGCTGAGGAAAGAAGAAGTTTCCCCGGAAGAAGTTTTAGA
TAGTTTTTATAAAAGAATAGATGAGGTTGAAGATAAAGTTAAAGCCTATGTGACCCTTACCAGAGATGAAGCCAGAAATA
GTCTTGCCAGTCTTAAGGATGGAAGACTGGCCGGTATTCCCCTGGCCATTAAAGATAATATTTCCACCAGAGGTATTAAA
ACCACCTGTTCTTCTAAAATACTGAACAACTATAAACCTCCATATGATGCTACTGTTGTTAAGAGATTAAAGGAAGAGGG
GGGTATTACCCTTGGTAAAACCAATATGGATGAGTTTGCCATGGGTTCATCTACTGAAAACTCTGGTTTTTATCCTACCC
ATAACCCCTGGAATTTAGACCATGCTCCCGGGGGCTCAAGTGGTGGTTCTGCAGCAGCTGTGGCTGCCGGTGAAGCACCA
GGTGCCCTTGGCTCTGATACAGGTGGTTCTATCCGTCAGCCAGCTGCATTTTGTGGTGTTGTGGGGTTAAAACCAACTTA
TGGTTGTGTTTCCCGTTATGGACTGGTGGCTTTTGCTTCTTCTCTGGATCAGATTGGACCTATTACAAAAGATGTGACTG
ATTCTGCCCTGTTATTGAATGTTATAAGTGGTCATGATCCGATGGATTCTACCTCTGTGGACCGGGAGAAGGAGGATTAT
ACTACATACCTGAAAGACGATGTCAAAGGTATGAAGATTGGTCTACCGGAAGAGTACTTCTCCCTTGATTTTAATTCTGA
AGTTAAAGATAAAGTTATGTCAGCTGTAAAAGAGCTTGAAAAAGCAGGGGCCATTGTAGAAGAAGTTAGCCTTCCCAATA
TTGAGTACGCCCTGGCTGCTTATTACATCATTGCTCCGGCTGAAGCCAGCTCCAACCTGGCCCGTTATGATGGAGTAAGG
TATGGTTACCGGAGTGAGAACGGTGATAGTGTCAGGTCCATGTTCACCAATACCAGGAGTGAGGGTTTTGGTGATGAAGT
CAAGAGGAGAATTATGCTGGGAACATATGTCTTAAGTTCCGGGTATTATGATGCCTTTTATTTGAAAGCCCAGAAAGTAA
GGACCCTTATTAAAGAGGATTTTGAAAGGGTCTTTAAGGACTATGATGTTTTGATTTCACCAACAACCCCGACCACAGCA
TTTAAGCTGGGAGAAATGACTGATCCCCTTGAAATGTATCAATCTGATGTCTTTACAGTTCCGGTAAATATTGCCGGGAT
ACCTGCTATTTCTGTTCCCTGTGGTTTTGACAGTAATAATCTTCCCATTGGACTCCAGATAATGGGACCCCATTTCGGGG
AAGGTAAGATTCTTCAGACTGCCTATACCCTGGAACAGGCTCTAAACATGAAGACAAAAAGGCCTAAGTTATGA

Upstream 100 bases:

>100_bases
TCAATTCCCAGGGAAGAGGCACTTGATAATGCGCCCGACAAAAAGGATGGTCTGTTCAGAGTACCCAGTATCATAGGTGA
ATAAAAAGGAGGTTATGTAA

Downstream 100 bases:

>100_bases
TAAAGTGGAGGTGTAATTAAATGAGCACAGAATACGAAACTATTATTGGACTTGAGGTCCATGTCCAGTTGTCTACCAAC
TCCAAGATCTTTTGTGATTG

Product: glutamyl-tRNA(Gln) amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MELYDLTIHELRDLLRKEEVSPEEVLDSFYKRIDEVEDKVKAYVTLTRDEARNSLASLKDGRLAGIPLAIKDNISTRGIK
TTCSSKILNNYKPPYDATVVKRLKEEGGITLGKTNMDEFAMGSSTENSGFYPTHNPWNLDHAPGGSSGGSAAAVAAGEAP
GALGSDTGGSIRQPAAFCGVVGLKPTYGCVSRYGLVAFASSLDQIGPITKDVTDSALLLNVISGHDPMDSTSVDREKEDY
TTYLKDDVKGMKIGLPEEYFSLDFNSEVKDKVMSAVKELEKAGAIVEEVSLPNIEYALAAYYIIAPAEASSNLARYDGVR
YGYRSENGDSVRSMFTNTRSEGFGDEVKRRIMLGTYVLSSGYYDAFYLKAQKVRTLIKEDFERVFKDYDVLISPTTPTTA
FKLGEMTDPLEMYQSDVFTVPVNIAGIPAISVPCGFDSNNLPIGLQIMGPHFGEGKILQTAYTLEQALNMKTKRPKL

Sequences:

>Translated_477_residues
MELYDLTIHELRDLLRKEEVSPEEVLDSFYKRIDEVEDKVKAYVTLTRDEARNSLASLKDGRLAGIPLAIKDNISTRGIK
TTCSSKILNNYKPPYDATVVKRLKEEGGITLGKTNMDEFAMGSSTENSGFYPTHNPWNLDHAPGGSSGGSAAAVAAGEAP
GALGSDTGGSIRQPAAFCGVVGLKPTYGCVSRYGLVAFASSLDQIGPITKDVTDSALLLNVISGHDPMDSTSVDREKEDY
TTYLKDDVKGMKIGLPEEYFSLDFNSEVKDKVMSAVKELEKAGAIVEEVSLPNIEYALAAYYIIAPAEASSNLARYDGVR
YGYRSENGDSVRSMFTNTRSEGFGDEVKRRIMLGTYVLSSGYYDAFYLKAQKVRTLIKEDFERVFKDYDVLISPTTPTTA
FKLGEMTDPLEMYQSDVFTVPVNIAGIPAISVPCGFDSNNLPIGLQIMGPHFGEGKILQTAYTLEQALNMKTKRPKL
>Mature_477_residues
MELYDLTIHELRDLLRKEEVSPEEVLDSFYKRIDEVEDKVKAYVTLTRDEARNSLASLKDGRLAGIPLAIKDNISTRGIK
TTCSSKILNNYKPPYDATVVKRLKEEGGITLGKTNMDEFAMGSSTENSGFYPTHNPWNLDHAPGGSSGGSAAAVAAGEAP
GALGSDTGGSIRQPAAFCGVVGLKPTYGCVSRYGLVAFASSLDQIGPITKDVTDSALLLNVISGHDPMDSTSVDREKEDY
TTYLKDDVKGMKIGLPEEYFSLDFNSEVKDKVMSAVKELEKAGAIVEEVSLPNIEYALAAYYIIAPAEASSNLARYDGVR
YGYRSENGDSVRSMFTNTRSEGFGDEVKRRIMLGTYVLSSGYYDAFYLKAQKVRTLIKEDFERVFKDYDVLISPTTPTTA
FKLGEMTDPLEMYQSDVFTVPVNIAGIPAISVPCGFDSNNLPIGLQIMGPHFGEGKILQTAYTLEQALNMKTKRPKL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=497, Percent_Identity=37.625754527163, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI195972892, Length=508, Percent_Identity=27.1653543307087, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI166795287, Length=202, Percent_Identity=31.6831683168317, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=432, Percent_Identity=39.5833333333333, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17537465, Length=491, Percent_Identity=25.4582484725051, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17556264, Length=260, Percent_Identity=30.7692307692308, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=316, Percent_Identity=25.6329113924051, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=285, Percent_Identity=28.4210526315789, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=316, Percent_Identity=28.1645569620253, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17556278, Length=316, Percent_Identity=28.1645569620253, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323950, Length=418, Percent_Identity=38.2775119617225, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=460, Percent_Identity=29.3478260869565, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320448, Length=529, Percent_Identity=23.8185255198488, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648113, Length=423, Percent_Identity=43.4988179669031, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24644968, Length=503, Percent_Identity=28.6282306163022, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24652985, Length=487, Percent_Identity=27.1047227926078, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19922090, Length=487, Percent_Identity=27.1047227926078, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24652981, Length=487, Percent_Identity=27.1047227926078, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24652983, Length=487, Percent_Identity=27.1047227926078, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI161078093, Length=503, Percent_Identity=27.2365805168986, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648435, Length=480, Percent_Identity=25.8333333333333, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648437, Length=480, Percent_Identity=25.8333333333333, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI45550774, Length=480, Percent_Identity=25.8333333333333, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648441, Length=414, Percent_Identity=26.8115942028986, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648439, Length=414, Percent_Identity=26.8115942028986, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356731, Length=496, Percent_Identity=23.7903225806452, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_HALOH (B8D125)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002507989.1
- ProteinModelPortal:   B8D125
- SMR:   B8D125
- GeneID:   7312553
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_02330
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52222; Mature: 52222

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 71-71 ACT_SITE 146-146 ACT_SITE 170-170

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELYDLTIHELRDLLRKEEVSPEEVLDSFYKRIDEVEDKVKAYVTLTRDEARNSLASLKD
CCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHCCC
GRLAGIPLAIKDNISTRGIKTTCSSKILNNYKPPYDATVVKRLKEEGGITLGKTNMDEFA
CCEECCCEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEECCCHHHHH
MGSSTENSGFYPTHNPWNLDHAPGGSSGGSAAAVAAGEAPGALGSDTGGSIRQPAAFCGV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHC
VGLKPTYGCVSRYGLVAFASSLDQIGPITKDVTDSALLLNVISGHDPMDSTSVDREKEDY
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHH
TTYLKDDVKGMKIGLPEEYFSLDFNSEVKDKVMSAVKELEKAGAIVEEVSLPNIEYALAA
HHHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHEEEE
YYIIAPAEASSNLARYDGVRYGYRSENGDSVRSMFTNTRSEGFGDEVKRRIMLGTYVLSS
EEEEECCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHC
GYYDAFYLKAQKVRTLIKEDFERVFKDYDVLISPTTPTTAFKLGEMTDPLEMYQSDVFTV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHEECCCCCCHHHHHCCCEEEE
PVNIAGIPAISVPCGFDSNNLPIGLQIMGPHFGEGKILQTAYTLEQALNMKTKRPKL
EEEECCCCEEEECCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MELYDLTIHELRDLLRKEEVSPEEVLDSFYKRIDEVEDKVKAYVTLTRDEARNSLASLKD
CCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHCCC
GRLAGIPLAIKDNISTRGIKTTCSSKILNNYKPPYDATVVKRLKEEGGITLGKTNMDEFA
CCEECCCEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEECCCHHHHH
MGSSTENSGFYPTHNPWNLDHAPGGSSGGSAAAVAAGEAPGALGSDTGGSIRQPAAFCGV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHC
VGLKPTYGCVSRYGLVAFASSLDQIGPITKDVTDSALLLNVISGHDPMDSTSVDREKEDY
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHH
TTYLKDDVKGMKIGLPEEYFSLDFNSEVKDKVMSAVKELEKAGAIVEEVSLPNIEYALAA
HHHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHEEEE
YYIIAPAEASSNLARYDGVRYGYRSENGDSVRSMFTNTRSEGFGDEVKRRIMLGTYVLSS
EEEEECCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHC
GYYDAFYLKAQKVRTLIKEDFERVFKDYDVLISPTTPTTAFKLGEMTDPLEMYQSDVFTV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHEECCCCCCHHHHHCCCEEEE
PVNIAGIPAISVPCGFDSNNLPIGLQIMGPHFGEGKILQTAYTLEQALNMKTKRPKL
EEEECCCCEEEECCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA