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The map label for this gene is gatB [H]

Identifier: 220931082

GI number: 220931082

Start: 240537

End: 241985

Strand: Direct

Name: gatB [H]

Synonym: Hore_02340

Alternate gene names: 220931082

Gene position: 240537-241985 (Clockwise)

Preceding gene: 220931081

Following gene: 220931083

Centisome position: 9.33

GC content: 43.82

Gene sequence:

>1449_bases
ATGAGCACAGAATACGAAACTATTATTGGACTTGAGGTCCATGTCCAGTTGTCTACCAACTCCAAGATCTTTTGTGATTG
TAGCACTGAATTTGGGGCAGAACCAAATACCCATACCTGTCCGGTCTGCCTGGGTTTGCCTGGAACCCTGCCGGTTTTAA
ATAAAAAGGCGGTTGATTATGCTATTATGGCCGGCCTGGCTCTTAATTGTGATATCAGTGAGTATAGTAAATTTGACCGG
AAGAATTATTTCTACCCTGATCTTCCTAAAGCATACCAGATTTCCCAGTATGACCTGCCCCTGTGTCAGAATGGCTACAT
AGAAATTGATACAGAAGATGGGGTTAAAAAGATAGGTATTACCAGGATTCATCTTGAGGAAGATGCCGGAAAACTTATTC
ATGAGGGGACAATAGATGAATCAGATTCCAGTCTGGTTGATTATAACAGGACGGGTGTTCCTTTAATTGAAATTGTCAGT
GAACCCGATATGAGAAGCCCATCTGAGGCCCGGGAGTACTTGACCCAGTTAAAAAAGGTTATGGAATATCTGGGGGTTTC
TGATTGTAATATGGAAGAGGGTTCCTTACGGTGTGATGCCAATGTGTCTATCAGGCCGGTTGGCCAGGAGGAATTTGGAA
CCAAAACCGAACTTAAAAATATGAACTCTTTCCGGGCTGTTGAAAGGGCCCTTGAATATGAAGTTGAAAGACAGAAAAAG
GTACTTGCCCGTGGAGATAAAGTAGTTCAGGAAACCAGAACCTGGGATGAGAAACTGAATAAAACAATCTCCATGAGAGG
TAAGGAAGAAGCCCATGACTACCGGTATTTCCCGGAACCTGACCTGGTTCCCCTGGAAGTTGATAAGGGATGGGTTGAGG
AAATTAAAAGTAAACTTCCGGAACTCCCCCGGGCCCGGAAGGAAAGGTTTGTAAAAGAGTATGGCCTGCCTGAATATGAT
GCCGGTGTCCTGACCGATTCCCGATCTCTGGCAGATTTCTTTGAAGAGGTTGTTAAAGCATATGATGACCCGAAAACTGT
TAGCAACTGGGTAATGGGAGAGTTTTTAAGGCTGATTAAAGAAGAAGGAATTGTCATAGAGGAGAGTAAGATTACCGGGG
AACTTCTGGCCCGGATGCTAAAATTACAGGATAAAGGGGTTATCAGCAGTAAGATAGCCAAGACAGTATTTGAAGAGATG
TTTAAAACAGGAAAGGACCCTGAAAAAATCGTAGAAGAAAAAGGTTTAAAACAGATCAGTGATGAGGGACAGCTTGAAGG
AATAGTGGAAAAGGTTATTGAGGAAAACCCTGAAGCTGTTGAAGATATCAGGGGAGGGAAGGGTAAGGCTATCGGCTATC
TGGTGGGTCAGGTTATGAAGGAAACCAGAGGCAAGGCCAATCCCCAGCTTGTAAACAAGTTGCTCAGGGAGAAAATAACC
GGCCAGTAA

Upstream 100 bases:

>100_bases
TCGGGGAAGGTAAGATTCTTCAGACTGCCTATACCCTGGAACAGGCTCTAAACATGAAGACAAAAAGGCCTAAGTTATGA
TAAAGTGGAGGTGTAATTAA

Downstream 100 bases:

>100_bases
AATTAAGTGAATAAATTGTGTATAAAATTAATTATTCCTGGAATATAGTTAAAATGAAAGAATCAGGATTATTAAATTAG
CCACCGGTAACATCCGGTGG

Product: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MSTEYETIIGLEVHVQLSTNSKIFCDCSTEFGAEPNTHTCPVCLGLPGTLPVLNKKAVDYAIMAGLALNCDISEYSKFDR
KNYFYPDLPKAYQISQYDLPLCQNGYIEIDTEDGVKKIGITRIHLEEDAGKLIHEGTIDESDSSLVDYNRTGVPLIEIVS
EPDMRSPSEAREYLTQLKKVMEYLGVSDCNMEEGSLRCDANVSIRPVGQEEFGTKTELKNMNSFRAVERALEYEVERQKK
VLARGDKVVQETRTWDEKLNKTISMRGKEEAHDYRYFPEPDLVPLEVDKGWVEEIKSKLPELPRARKERFVKEYGLPEYD
AGVLTDSRSLADFFEEVVKAYDDPKTVSNWVMGEFLRLIKEEGIVIEESKITGELLARMLKLQDKGVISSKIAKTVFEEM
FKTGKDPEKIVEEKGLKQISDEGQLEGIVEKVIEENPEAVEDIRGGKGKAIGYLVGQVMKETRGKANPQLVNKLLREKIT
GQ

Sequences:

>Translated_482_residues
MSTEYETIIGLEVHVQLSTNSKIFCDCSTEFGAEPNTHTCPVCLGLPGTLPVLNKKAVDYAIMAGLALNCDISEYSKFDR
KNYFYPDLPKAYQISQYDLPLCQNGYIEIDTEDGVKKIGITRIHLEEDAGKLIHEGTIDESDSSLVDYNRTGVPLIEIVS
EPDMRSPSEAREYLTQLKKVMEYLGVSDCNMEEGSLRCDANVSIRPVGQEEFGTKTELKNMNSFRAVERALEYEVERQKK
VLARGDKVVQETRTWDEKLNKTISMRGKEEAHDYRYFPEPDLVPLEVDKGWVEEIKSKLPELPRARKERFVKEYGLPEYD
AGVLTDSRSLADFFEEVVKAYDDPKTVSNWVMGEFLRLIKEEGIVIEESKITGELLARMLKLQDKGVISSKIAKTVFEEM
FKTGKDPEKIVEEKGLKQISDEGQLEGIVEKVIEENPEAVEDIRGGKGKAIGYLVGQVMKETRGKANPQLVNKLLREKIT
GQ
>Mature_481_residues
STEYETIIGLEVHVQLSTNSKIFCDCSTEFGAEPNTHTCPVCLGLPGTLPVLNKKAVDYAIMAGLALNCDISEYSKFDRK
NYFYPDLPKAYQISQYDLPLCQNGYIEIDTEDGVKKIGITRIHLEEDAGKLIHEGTIDESDSSLVDYNRTGVPLIEIVSE
PDMRSPSEAREYLTQLKKVMEYLGVSDCNMEEGSLRCDANVSIRPVGQEEFGTKTELKNMNSFRAVERALEYEVERQKKV
LARGDKVVQETRTWDEKLNKTISMRGKEEAHDYRYFPEPDLVPLEVDKGWVEEIKSKLPELPRARKERFVKEYGLPEYDA
GVLTDSRSLADFFEEVVKAYDDPKTVSNWVMGEFLRLIKEEGIVIEESKITGELLARMLKLQDKGVISSKIAKTVFEEMF
KTGKDPEKIVEEKGLKQISDEGQLEGIVEKVIEENPEAVEDIRGGKGKAIGYLVGQVMKETRGKANPQLVNKLLREKITG
Q

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=499, Percent_Identity=39.6793587174349, Blast_Score=367, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI115534094, Length=475, Percent_Identity=31.5789473684211, Blast_Score=225, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6319391, Length=524, Percent_Identity=29.5801526717557, Blast_Score=231, Evalue=3e-61,
Organism=Drosophila melanogaster, GI45550808, Length=486, Percent_Identity=34.156378600823, Blast_Score=296, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54537; Mature: 54406

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEYETIIGLEVHVQLSTNSKIFCDCSTEFGAEPNTHTCPVCLGLPGTLPVLNKKAVDY
CCCCCCEEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHH
AIMAGLALNCDISEYSKFDRKNYFYPDLPKAYQISQYDLPLCQNGYIEIDTEDGVKKIGI
HHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCEEEECCCCHHHHCE
TRIHLEEDAGKLIHEGTIDESDSSLVDYNRTGVPLIEIVSEPDMRSPSEAREYLTQLKKV
EEEEECCHHHHHHCCCCCCCCCCCEECCCCCCCCHHEEECCCCCCCHHHHHHHHHHHHHH
MEYLGVSDCNMEEGSLRCDANVSIRPVGQEEFGTKTELKNMNSFRAVERALEYEVERQKK
HHHHCCCCCCCCCCCEEECCCCEEEECCHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
VLARGDKVVQETRTWDEKLNKTISMRGKEEAHDYRYFPEPDLVPLEVDKGWVEEIKSKLP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEECCCHHHHHHHHHCC
ELPRARKERFVKEYGLPEYDAGVLTDSRSLADFFEEVVKAYDDPKTVSNWVMGEFLRLIK
CCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EEGIVIEESKITGELLARMLKLQDKGVISSKIAKTVFEEMFKTGKDPEKIVEEKGLKQIS
HCCCEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
DEGQLEGIVEKVIEENPEAVEDIRGGKGKAIGYLVGQVMKETRGKANPQLVNKLLREKIT
CCHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
GQ
CC
>Mature Secondary Structure 
STEYETIIGLEVHVQLSTNSKIFCDCSTEFGAEPNTHTCPVCLGLPGTLPVLNKKAVDY
CCCCCEEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHH
AIMAGLALNCDISEYSKFDRKNYFYPDLPKAYQISQYDLPLCQNGYIEIDTEDGVKKIGI
HHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCEEEECCCCHHHHCE
TRIHLEEDAGKLIHEGTIDESDSSLVDYNRTGVPLIEIVSEPDMRSPSEAREYLTQLKKV
EEEEECCHHHHHHCCCCCCCCCCCEECCCCCCCCHHEEECCCCCCCHHHHHHHHHHHHHH
MEYLGVSDCNMEEGSLRCDANVSIRPVGQEEFGTKTELKNMNSFRAVERALEYEVERQKK
HHHHCCCCCCCCCCCEEECCCCEEEECCHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
VLARGDKVVQETRTWDEKLNKTISMRGKEEAHDYRYFPEPDLVPLEVDKGWVEEIKSKLP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEECCCHHHHHHHHHCC
ELPRARKERFVKEYGLPEYDAGVLTDSRSLADFFEEVVKAYDDPKTVSNWVMGEFLRLIK
CCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EEGIVIEESKITGELLARMLKLQDKGVISSKIAKTVFEEMFKTGKDPEKIVEEKGLKQIS
HCCCEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
DEGQLEGIVEKVIEENPEAVEDIRGGKGKAIGYLVGQVMKETRGKANPQLVNKLLREKIT
CCHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
GQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA