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The map label for this gene is proB [H]

Identifier: 220931041

GI number: 220931041

Start: 198239

End: 199402

Strand: Direct

Name: proB [H]

Synonym: Hore_01920

Alternate gene names: 220931041

Gene position: 198239-199402 (Clockwise)

Preceding gene: 220931040

Following gene: 220931042

Centisome position: 7.69

GC content: 44.93

Gene sequence:

>1164_bases
ATGGGCAGGGGACGAAAGAAGAAATATAACCGGGTGGTTGTTAAGGTTGGTAGCAGTACTTTAACCCACCCGGGTGGAAA
ATTAAATATTACCAGGATCGACCATCTTGTCAGGCAGCTGGTAGATTTGAAGAACCAGGGGCGGGAAGTCCTGCTTGTGA
CTTCAGGTGCTATTGCTGCTGGAATGGGAGATATGAACCTTGATGTCAGGCCCGGTTCTATTCCCGAACAGCAGGCCCTG
GCTGCCCTCGGTCAGGGAGTATTAATCGGATTATATAATAAGTTTTTCAGGGAATATGGAGAAAAAGGGGCCCAGATATT
ATTAACTGCCAGTGACCTGGAAGACAGGGGGCGGTATTTAAATGCCTTTAATACCCTGTTAACTTTATTAAAACACGGGG
TTATCCCCGTAATAAATGAGAATGATACTGTTGCTACCCAGGAAATTAAATTTGGCGATAATGACACCCTGTCGGCCCGG
GTAGCCGGACTCGTTGAGGCTGATCTTTTGATCGTTCTGTCAGATGTTGAAGGCCTGTATAACGGGGTTCCTGGAGAGGG
ATCAGACCTTGAAGTTATTAGAGTAGTAGAGGACATTACTCCTGACGTTGAAGGGCTGGCCGGTGGGCGGGGTAGCTCTG
TCGGGACCGGGGGTATGATTACCAAGATAAAAGCTGCCAGGATTGCCGTTAATTCCGGGGTTACTATGGTTATCGGACCC
GGTTATGAGAAGAACGTTTTATTATCACTGGTAGATATGCTGGAAAAGGGAAATAATTATAATAAAGGGACAACTTTTCT
TCCAAAACAGGATTACCTGACTAAACGAAAACAGTGGTTGCGCTTTAACCTGCCGGTCAGTGGTTCTATAAAAGTTGATA
AAGGTGCTGAAACTGCCCTGGTATACCGGGGTAAAAGTCTTTTACCCGGTGGTATAACCGGGGTTGAGGGAGATTTTTCA
GAAGGTGAACCGGTTAGGGTAATCAACCACAGTGGTAAAGAGATAGGGAGAGGGTTGGTTAATTATTCATCTGAAGAAAT
TAGAGTTATTCAGGGACACCACACCACAGATATTAGTTCTTTACTCGGTTATATGAAACAGGAAGAAGTAATACACCGGG
ATAACCTGGTAATAGAAAGGGGTAAGATTGATAATGGAGATTAA

Upstream 100 bases:

>100_bases
CCTTTATCAGGGGCCTTAAATTGACTCTGGAATAACTATTTGCTATAATTAATAAATATACAAATCAAGATATTAAGGAA
ATAGACCAGGGGATGATTAT

Downstream 100 bases:

>100_bases
AGAACAGGTGGTATTTCAGGCCCGGAAAGCCAGTAAGGCTTCCCGGAAACTTTCCGGTATTGCAACCGATGTTAAAAATA
AGGCCCTGGCTGCCATTGCA

Product: glutamate 5-kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MGRGRKKKYNRVVVKVGSSTLTHPGGKLNITRIDHLVRQLVDLKNQGREVLLVTSGAIAAGMGDMNLDVRPGSIPEQQAL
AALGQGVLIGLYNKFFREYGEKGAQILLTASDLEDRGRYLNAFNTLLTLLKHGVIPVINENDTVATQEIKFGDNDTLSAR
VAGLVEADLLIVLSDVEGLYNGVPGEGSDLEVIRVVEDITPDVEGLAGGRGSSVGTGGMITKIKAARIAVNSGVTMVIGP
GYEKNVLLSLVDMLEKGNNYNKGTTFLPKQDYLTKRKQWLRFNLPVSGSIKVDKGAETALVYRGKSLLPGGITGVEGDFS
EGEPVRVINHSGKEIGRGLVNYSSEEIRVIQGHHTTDISSLLGYMKQEEVIHRDNLVIERGKIDNGD

Sequences:

>Translated_387_residues
MGRGRKKKYNRVVVKVGSSTLTHPGGKLNITRIDHLVRQLVDLKNQGREVLLVTSGAIAAGMGDMNLDVRPGSIPEQQAL
AALGQGVLIGLYNKFFREYGEKGAQILLTASDLEDRGRYLNAFNTLLTLLKHGVIPVINENDTVATQEIKFGDNDTLSAR
VAGLVEADLLIVLSDVEGLYNGVPGEGSDLEVIRVVEDITPDVEGLAGGRGSSVGTGGMITKIKAARIAVNSGVTMVIGP
GYEKNVLLSLVDMLEKGNNYNKGTTFLPKQDYLTKRKQWLRFNLPVSGSIKVDKGAETALVYRGKSLLPGGITGVEGDFS
EGEPVRVINHSGKEIGRGLVNYSSEEIRVIQGHHTTDISSLLGYMKQEEVIHRDNLVIERGKIDNGD
>Mature_386_residues
GRGRKKKYNRVVVKVGSSTLTHPGGKLNITRIDHLVRQLVDLKNQGREVLLVTSGAIAAGMGDMNLDVRPGSIPEQQALA
ALGQGVLIGLYNKFFREYGEKGAQILLTASDLEDRGRYLNAFNTLLTLLKHGVIPVINENDTVATQEIKFGDNDTLSARV
AGLVEADLLIVLSDVEGLYNGVPGEGSDLEVIRVVEDITPDVEGLAGGRGSSVGTGGMITKIKAARIAVNSGVTMVIGPG
YEKNVLLSLVDMLEKGNNYNKGTTFLPKQDYLTKRKQWLRFNLPVSGSIKVDKGAETALVYRGKSLLPGGITGVEGDFSE
GEPVRVINHSGKEIGRGLVNYSSEEIRVIQGHHTTDISSLLGYMKQEEVIHRDNLVIERGKIDNGD

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=274, Percent_Identity=35.7664233576642, Blast_Score=141, Evalue=9e-34,
Organism=Homo sapiens, GI21361368, Length=276, Percent_Identity=35.5072463768116, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=43.1693989071038, Blast_Score=280, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17569969, Length=266, Percent_Identity=37.218045112782, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17569967, Length=266, Percent_Identity=37.218045112782, Blast_Score=150, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320506, Length=425, Percent_Identity=36.4705882352941, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6321822, Length=418, Percent_Identity=35.8851674641148, Blast_Score=208, Evalue=1e-54,
Organism=Drosophila melanogaster, GI21357643, Length=294, Percent_Identity=34.6938775510204, Blast_Score=149, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 41784; Mature: 41653

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRGRKKKYNRVVVKVGSSTLTHPGGKLNITRIDHLVRQLVDLKNQGREVLLVTSGAIAA
CCCCCCCCCCEEEEEECCCEECCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCEE
GMGDMNLDVRPGSIPEQQALAALGQGVLIGLYNKFFREYGEKGAQILLTASDLEDRGRYL
CCCCCEEEECCCCCCHHHHHHHHCCCCEEHHHHHHHHHHCCCCCEEEEEECCHHHHHHHH
NAFNTLLTLLKHGVIPVINENDTVATQEIKFGDNDTLSARVAGLVEADLLIVLSDVEGLY
HHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHCEEEEEEHHHHHH
NGVPGEGSDLEVIRVVEDITPDVEGLAGGRGSSVGTGGMITKIKAARIAVNSGVTMVIGP
CCCCCCCCCHHEEEEHHHCCCCHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCEEEEECC
GYEKNVLLSLVDMLEKGNNYNKGTTFLPKQDYLTKRKQWLRFNLPVSGSIKVDKGAETAL
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCEEECCCCCEEE
VYRGKSLLPGGITGVEGDFSEGEPVRVINHSGKEIGRGLVNYSSEEIRVIQGHHTTDISS
EECCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCEEEEECCCCCCHHH
LLGYMKQEEVIHRDNLVIERGKIDNGD
HHHHHHHHHHHHCCCEEEECCCCCCCC
>Mature Secondary Structure 
GRGRKKKYNRVVVKVGSSTLTHPGGKLNITRIDHLVRQLVDLKNQGREVLLVTSGAIAA
CCCCCCCCCEEEEEECCCEECCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCEE
GMGDMNLDVRPGSIPEQQALAALGQGVLIGLYNKFFREYGEKGAQILLTASDLEDRGRYL
CCCCCEEEECCCCCCHHHHHHHHCCCCEEHHHHHHHHHHCCCCCEEEEEECCHHHHHHHH
NAFNTLLTLLKHGVIPVINENDTVATQEIKFGDNDTLSARVAGLVEADLLIVLSDVEGLY
HHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHCEEEEEEHHHHHH
NGVPGEGSDLEVIRVVEDITPDVEGLAGGRGSSVGTGGMITKIKAARIAVNSGVTMVIGP
CCCCCCCCCHHEEEEHHHCCCCHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCEEEEECC
GYEKNVLLSLVDMLEKGNNYNKGTTFLPKQDYLTKRKQWLRFNLPVSGSIKVDKGAETAL
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCEEECCCCCEEE
VYRGKSLLPGGITGVEGDFSEGEPVRVINHSGKEIGRGLVNYSSEEIRVIQGHHTTDISS
EECCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCEEEEECCCCCCHHH
LLGYMKQEEVIHRDNLVIERGKIDNGD
HHHHHHHHHHHHCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]