Click here to switch to the map view.

The map label for this gene is amiC

Identifier: 221230313

GI number: 221230313

Start: 1920839

End: 1922245

Strand: Reverse

Name: amiC

Synonym: MLBr_01596

Alternate gene names: 221230313

Gene position: 1922245-1920839 (Counterclockwise)

Preceding gene: 221230314

Following gene: 221230312

Centisome position: 58.82

GC content: 62.62

Gene sequence:

>1407_bases
ATGCAACGTGTGCACGCTTTCGGTGACGACGCCCTCGGCGATCTCGACGCGGTCGGTCTAGCCGACGCTATCCGAGCCGG
TTGGGTCAGCAGAGCCGATGTCATCGAGGCGGCCATTGTGCGGACCGAGGCCGTTAACCCGGCCTTGGGTGGCTTGGCTT
ATGAGGCGTTTCAGTGGGCTCGGCAGACCGCGTCGAAAGCAGGCAGTGGCTTCTTTAGCGGTGTCCCGACCTTCATCAAG
GACAACATCGATGTCGCTGGACAGCCGACGATGCGTGGCAGCGACGCATGGGTGCCCCGAAATGCGTTCGACGACGGAGA
GTTCACCCGGCTGTATTTGGCCACCGGACCGGTATCTTTGGGCAAGACGCAGTTGTCAGAATTCGGGTTCAGCGCCTCCG
CTGAACATATGCGTCTAGGGCCGGTTCGCAACCCATGGGACACCGACTACACTGCCGGGGCCTCGTCTTCGGGATCGGGT
GCGTTCGTCGCCGCCGGTGTAGTCCCGATGGCGCACGCCAACGATGGTGGCGGGTCGATTCGTATTCCGGCCTCCTGCAA
CGGGCTTGTCGGGCTCAAGCCGTCACGCGGACGATTGCCGCTGGATTCCGAGCTGCGCCGGCTTCCGGTGGGCATCGTAG
TCAACGGCGTGCTGACCCGCTCGGTGCGGGACACTGCGGCCTTCTATAGAGAGGCTGAGCGTATTTGGCACAACCCCAAG
CTGCCGCCGGTCGGAGACGTCACGCAGCCCGGTCGGCAACGGCTTCGGATTGCCGTGGTGACGCGTTCGGTTCAGCGCGA
GTGTAGCCCCGAACTACGCGAGCTCACACTGAAGTCGGCTAGGCTGCTCGAGGAGCTGGGCCATCGCGTCGAACGGGTTG
CCGAGCCCCCGGTGCCGCCTAATTTCCCGGACGATTTCCTGCTGTATTGGGGACTGTTGGCGGCGATGCAGGTGCGGACC
GGCCGGCTCGCGTTCGGCAACACGTTCGACCGCACCAAGCTGGACAGTTTGACGCTGGGCCTGGATCGCCACGCCAGCCG
CAACATGCACCGGCTCCCCAAGGCAATCATGCGTTTGCGTAGGCTGCGGCGTCGCACCGCTGACTTTTTCGCTACCTACG
ATGTATTACTTACTCCAACGGTCGCTGACGAGACACCGCGGATAGGGTACCTCACTCCCACCGACTATCAGCAGGTGATG
GACCGGCTGATGGGCTGGGTCGCTTTCACTCCGTTGCAAAACGTCACTGGGGAGCCGGCGATTTCGCTGCCGTTGGCCCA
ATCCGCTGATGGTATGCCGGTGGGCATGATGTTCACCGCTGACTTTGGTCAGGAAGCGCAACTATTGGAACTGGCATTTG
AACTCGAAGAGGCTCGGCCGTGGGCGCGGATTCAAATTGGCGACTAG

Upstream 100 bases:

>100_bases
TCCTGCTTGACGAGGCCGGCGTGACGGTGACGCGATTCGTTCGGTTCGAGGTGGGCCAAGCCTAGCTTGGCGGTGAGCGC
GAGCGTCTAGCGTTGTTGCT

Downstream 100 bases:

>100_bases
CTGTCAGTTGGATCCGAGCTGGTAGAGCATTCGCTTGAACTCACGCTGCTGCGGTGCACCCAGTTTGTCTAAGATGCGGC
CATCAGCTATGCGGATCGCA

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MQRVHAFGDDALGDLDAVGLADAIRAGWVSRADVIEAAIVRTEAVNPALGGLAYEAFQWARQTASKAGSGFFSGVPTFIK
DNIDVAGQPTMRGSDAWVPRNAFDDGEFTRLYLATGPVSLGKTQLSEFGFSASAEHMRLGPVRNPWDTDYTAGASSSGSG
AFVAAGVVPMAHANDGGGSIRIPASCNGLVGLKPSRGRLPLDSELRRLPVGIVVNGVLTRSVRDTAAFYREAERIWHNPK
LPPVGDVTQPGRQRLRIAVVTRSVQRECSPELRELTLKSARLLEELGHRVERVAEPPVPPNFPDDFLLYWGLLAAMQVRT
GRLAFGNTFDRTKLDSLTLGLDRHASRNMHRLPKAIMRLRRLRRRTADFFATYDVLLTPTVADETPRIGYLTPTDYQQVM
DRLMGWVAFTPLQNVTGEPAISLPLAQSADGMPVGMMFTADFGQEAQLLELAFELEEARPWARIQIGD

Sequences:

>Translated_468_residues
MQRVHAFGDDALGDLDAVGLADAIRAGWVSRADVIEAAIVRTEAVNPALGGLAYEAFQWARQTASKAGSGFFSGVPTFIK
DNIDVAGQPTMRGSDAWVPRNAFDDGEFTRLYLATGPVSLGKTQLSEFGFSASAEHMRLGPVRNPWDTDYTAGASSSGSG
AFVAAGVVPMAHANDGGGSIRIPASCNGLVGLKPSRGRLPLDSELRRLPVGIVVNGVLTRSVRDTAAFYREAERIWHNPK
LPPVGDVTQPGRQRLRIAVVTRSVQRECSPELRELTLKSARLLEELGHRVERVAEPPVPPNFPDDFLLYWGLLAAMQVRT
GRLAFGNTFDRTKLDSLTLGLDRHASRNMHRLPKAIMRLRRLRRRTADFFATYDVLLTPTVADETPRIGYLTPTDYQQVM
DRLMGWVAFTPLQNVTGEPAISLPLAQSADGMPVGMMFTADFGQEAQLLELAFELEEARPWARIQIGD
>Mature_468_residues
MQRVHAFGDDALGDLDAVGLADAIRAGWVSRADVIEAAIVRTEAVNPALGGLAYEAFQWARQTASKAGSGFFSGVPTFIK
DNIDVAGQPTMRGSDAWVPRNAFDDGEFTRLYLATGPVSLGKTQLSEFGFSASAEHMRLGPVRNPWDTDYTAGASSSGSG
AFVAAGVVPMAHANDGGGSIRIPASCNGLVGLKPSRGRLPLDSELRRLPVGIVVNGVLTRSVRDTAAFYREAERIWHNPK
LPPVGDVTQPGRQRLRIAVVTRSVQRECSPELRELTLKSARLLEELGHRVERVAEPPVPPNFPDDFLLYWGLLAAMQVRT
GRLAFGNTFDRTKLDSLTLGLDRHASRNMHRLPKAIMRLRRLRRRTADFFATYDVLLTPTVADETPRIGYLTPTDYQQVM
DRLMGWVAFTPLQNVTGEPAISLPLAQSADGMPVGMMFTADFGQEAQLLELAFELEEARPWARIQIGD

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=515, Percent_Identity=25.631067961165, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI195972892, Length=235, Percent_Identity=29.3617021276596, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=325, Percent_Identity=27.3846153846154, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=450, Percent_Identity=24.2222222222222, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=498, Percent_Identity=22.6907630522088, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=296, Percent_Identity=24.6621621621622, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=127, Percent_Identity=37.7952755905512, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=436, Percent_Identity=24.0825688073394, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI21356731, Length=473, Percent_Identity=26.0042283298097, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24652985, Length=512, Percent_Identity=26.953125, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922090, Length=512, Percent_Identity=26.953125, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24652981, Length=512, Percent_Identity=26.953125, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24652983, Length=512, Percent_Identity=26.953125, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648113, Length=439, Percent_Identity=28.4738041002278, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45550774, Length=199, Percent_Identity=28.643216080402, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648435, Length=199, Percent_Identity=28.643216080402, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24648437, Length=199, Percent_Identity=28.643216080402, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24644968, Length=198, Percent_Identity=29.2929292929293, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI161078093, Length=480, Percent_Identity=23.125, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMI3_MYCLE (O33040)

Other databases:

- EMBL:   Z97369
- EMBL:   AL583922
- PIR:   F87108
- RefSeq:   NP_302099.1
- ProteinModelPortal:   O33040
- SMR:   O33040
- EnsemblBacteria:   EBMYCT00000028682
- GeneID:   908490
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1596
- NMPDR:   fig|272631.1.peg.971
- Leproma:   ML1596
- GeneTree:   EBGT00050000014631
- HOGENOM:   HBG481888
- OMA:   NTDHTAG
- ProtClustDB:   PRK07869
- BioCyc:   MLEP272631:ML1596-MONOMER
- BRENDA:   3.5.1.4
- InterPro:   IPR000120
- InterPro:   IPR020556
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: =3.5.1.4

Molecular weight: Translated: 51165; Mature: 51165

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 80-80 ACT_SITE 155-155 ACT_SITE 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRVHAFGDDALGDLDAVGLADAIRAGWVSRADVIEAAIVRTEAVNPALGGLAYEAFQWA
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
RQTASKAGSGFFSGVPTFIKDNIDVAGQPTMRGSDAWVPRNAFDDGEFTRLYLATGPVSL
HHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
GKTQLSEFGFSASAEHMRLGPVRNPWDTDYTAGASSSGSGAFVAAGVVPMAHANDGGGSI
CHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCCEE
RIPASCNGLVGLKPSRGRLPLDSELRRLPVGIVVNGVLTRSVRDTAAFYREAERIWHNPK
EECCCCCCEEECCCCCCCCCCCHHHHHCCHHHEEHHHHHHHHHHHHHHHHHHHHHHCCCC
LPPVGDVTQPGRQRLRIAVVTRSVQRECSPELRELTLKSARLLEELGHRVERVAEPPVPP
CCCCCCCCCCHHHHEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NFPDDFLLYWGLLAAMQVRTGRLAFGNTFDRTKLDSLTLGLDRHASRNMHRLPKAIMRLR
CCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHEECCHHHHCCCHHHHHHHHHHHH
RLRRRTADFFATYDVLLTPTVADETPRIGYLTPTDYQQVMDRLMGWVAFTPLQNVTGEPA
HHHHHHHHHHEEEHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCCC
ISLPLAQSADGMPVGMMFTADFGQEAQLLELAFELEEARPWARIQIGD
EECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure
MQRVHAFGDDALGDLDAVGLADAIRAGWVSRADVIEAAIVRTEAVNPALGGLAYEAFQWA
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
RQTASKAGSGFFSGVPTFIKDNIDVAGQPTMRGSDAWVPRNAFDDGEFTRLYLATGPVSL
HHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
GKTQLSEFGFSASAEHMRLGPVRNPWDTDYTAGASSSGSGAFVAAGVVPMAHANDGGGSI
CHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCCEE
RIPASCNGLVGLKPSRGRLPLDSELRRLPVGIVVNGVLTRSVRDTAAFYREAERIWHNPK
EECCCCCCEEECCCCCCCCCCCHHHHHCCHHHEEHHHHHHHHHHHHHHHHHHHHHHCCCC
LPPVGDVTQPGRQRLRIAVVTRSVQRECSPELRELTLKSARLLEELGHRVERVAEPPVPP
CCCCCCCCCCHHHHEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NFPDDFLLYWGLLAAMQVRTGRLAFGNTFDRTKLDSLTLGLDRHASRNMHRLPKAIMRLR
CCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHEECCHHHHCCCHHHHHHHHHHHH
RLRRRTADFFATYDVLLTPTVADETPRIGYLTPTDYQQVMDRLMGWVAFTPLQNVTGEPA
HHHHHHHHHHEEEHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCCC
ISLPLAQSADGMPVGMMFTADFGQEAQLLELAFELEEARPWARIQIGD
EECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002