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The map label for this gene is tsf

Identifier: 221230314

GI number: 221230314

Start: 1922281

End: 1923111

Strand: Reverse

Name: tsf

Synonym: MLBr_01597

Alternate gene names: 221230314

Gene position: 1923111-1922281 (Counterclockwise)

Preceding gene: 221230315

Following gene: 221230313

Centisome position: 58.85

GC content: 60.41

Gene sequence:

>831_bases
ATGGCGAATTTTACTGTCGCTGATGTCAAGCGGCTTCGCGCGCTAACTGGTGCCGGCATGCTCGACTGCAAGAGTGTGCT
GGTCGAAACCGACGGAAACTTCGACAAGGCTGTCGAGTCTCTCCGGATCAAGGGTGCCAAGGACGTTGGCAAGCGTGCCG
AACGGGCCACTGCCGAGGGCCTGGTTGCGGCTAAAGATGGTGCGCTTATCGAGTTGAACTGCGAAACGGACTTCGTTGCT
AAAAACGCCGAGTTCCAAAAGCTGGCCAACCAAATCGTTGGGGTGGTGGCGGCAGCCAAAATCGTTGACGTCGACGCACT
CAAGGGGGCTAGTGTCGGTGATAAGACGGTCGAGCAAGCGATCGCCGAGCTGGCGGCCAAGATTGGCGAGAAACTGAAAC
TGCGCCGCGCCGCGATCTTCAACGGCACCGTGGCAACCTACCTACACAAGCGCGCGGCTGACTTGCCGCCAGCGGTGGGC
GTGCTAGTTGAGTATGGTGCCGGCACCGACGCGGCTAACAGCACAGCGGCGGCACATGCGGCCGCGCTGCAGATCGCCGC
GCTCAAGGCGCGTTTCCTGTCCCGCGACGATGTTCCCGAAGACGTCTTGGCTAGTGAACGTCGCATAGCCGAAGAGACCG
CTAAAGCTGCGGGCAAGCCGGAGCAGTCGCTGCCCAAGATCGTCGAAGGCCGGCTAAATGGTTTCTTCAAGGATTCCGTG
CTGCTCGAACAGCCGTCGGTTTTCGACAACAAGAAGACCGTCAAGGTCCTGCTTGACGAGGCCGGCGTGACGGTGACGCG
ATTCGTTCGGTTCGAGGTGGGCCAAGCCTAG

Upstream 100 bases:

>100_bases
CTCCCGGAATGGGAGCAGGAGCTGTTAGCCGGTGCTGGTTCCAGCGCCCTCAACGATTCGGGCGCGGACTTATCAGAAGC
CAACCCTACGGAAGCCTGAT

Downstream 100 bases:

>100_bases
CTTGGCGGTGAGCGCGAGCGTCTAGCGTTGTTGCTATGCAACGTGTGCACGCTTTCGGTGACGACGCCCTCGGCGATCTC
GACGCGGTCGGTCTAGCCGA

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MANFTVADVKRLRALTGAGMLDCKSVLVETDGNFDKAVESLRIKGAKDVGKRAERATAEGLVAAKDGALIELNCETDFVA
KNAEFQKLANQIVGVVAAAKIVDVDALKGASVGDKTVEQAIAELAAKIGEKLKLRRAAIFNGTVATYLHKRAADLPPAVG
VLVEYGAGTDAANSTAAAHAAALQIAALKARFLSRDDVPEDVLASERRIAEETAKAAGKPEQSLPKIVEGRLNGFFKDSV
LLEQPSVFDNKKTVKVLLDEAGVTVTRFVRFEVGQA

Sequences:

>Translated_276_residues
MANFTVADVKRLRALTGAGMLDCKSVLVETDGNFDKAVESLRIKGAKDVGKRAERATAEGLVAAKDGALIELNCETDFVA
KNAEFQKLANQIVGVVAAAKIVDVDALKGASVGDKTVEQAIAELAAKIGEKLKLRRAAIFNGTVATYLHKRAADLPPAVG
VLVEYGAGTDAANSTAAAHAAALQIAALKARFLSRDDVPEDVLASERRIAEETAKAAGKPEQSLPKIVEGRLNGFFKDSV
LLEQPSVFDNKKTVKVLLDEAGVTVTRFVRFEVGQA
>Mature_275_residues
ANFTVADVKRLRALTGAGMLDCKSVLVETDGNFDKAVESLRIKGAKDVGKRAERATAEGLVAAKDGALIELNCETDFVAK
NAEFQKLANQIVGVVAAAKIVDVDALKGASVGDKTVEQAIAELAAKIGEKLKLRRAAIFNGTVATYLHKRAADLPPAVGV
LVEYGAGTDAANSTAAAHAAALQIAALKARFLSRDDVPEDVLASERRIAEETAKAAGKPEQSLPKIVEGRLNGFFKDSVL
LEQPSVFDNKKTVKVLLDEAGVTVTRFVRFEVGQA

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Escherichia coli, GI1786366, Length=280, Percent_Identity=37.8571428571429, Blast_Score=165, Evalue=2e-42,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_MYCLB (B8ZRV3)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503730.1
- ProteinModelPortal:   B8ZRV3
- SMR:   B8ZRV3
- EnsemblBacteria:   EBMYCT00000085749
- GeneID:   7325764
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016347
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- ProtClustDB:   PRK09377
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 29157; Mature: 29026

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANFTVADVKRLRALTGAGMLDCKSVLVETDGNFDKAVESLRIKGAKDVGKRAERATAEG
CCCCCHHHHHHHHHHHCCCHHHHHHHHEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHC
LVAAKDGALIELNCETDFVAKNAEFQKLANQIVGVVAAAKIVDVDALKGASVGDKTVEQA
EEEECCCCEEEEECCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
IAELAAKIGEKLKLRRAAIFNGTVATYLHKRAADLPPAVGVLVEYGAGTDAANSTAAAHA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHEECCCCCCCCCCHHHHHH
AALQIAALKARFLSRDDVPEDVLASERRIAEETAKAAGKPEQSLPKIVEGRLNGFFKDSV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCE
LLEQPSVFDNKKTVKVLLDEAGVTVTRFVRFEVGQA
EECCCCCCCCCCCEEEEHHCCCCEEEHHEEHHCCCC
>Mature Secondary Structure 
ANFTVADVKRLRALTGAGMLDCKSVLVETDGNFDKAVESLRIKGAKDVGKRAERATAEG
CCCCHHHHHHHHHHHCCCHHHHHHHHEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHC
LVAAKDGALIELNCETDFVAKNAEFQKLANQIVGVVAAAKIVDVDALKGASVGDKTVEQA
EEEECCCCEEEEECCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
IAELAAKIGEKLKLRRAAIFNGTVATYLHKRAADLPPAVGVLVEYGAGTDAANSTAAAHA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHEECCCCCCCCCCHHHHHH
AALQIAALKARFLSRDDVPEDVLASERRIAEETAKAAGKPEQSLPKIVEGRLNGFFKDSV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCE
LLEQPSVFDNKKTVKVLLDEAGVTVTRFVRFEVGQA
EECCCCCCCCCCCEEEEHHCCCCEEEHHEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA