Click here to switch to the map view.

The map label for this gene is dapA

Identifier: 221230274

GI number: 221230274

Start: 1824550

End: 1825452

Strand: Reverse

Name: dapA

Synonym: MLBr_01513

Alternate gene names: 221230274

Gene position: 1825452-1824550 (Counterclockwise)

Preceding gene: 221230275

Following gene: 221230273

Centisome position: 55.86

GC content: 61.35

Gene sequence:

>903_bases
GTGACCACTGTCGGATTCGACGTCCCCGCACGTTTGGGGACCCTGCTTACTGCGATGGTGACACCGTTTGACGCTGATGG
TTCTGTTGACACTGCGGCTGCGACGCGGCTGGCGAACCGCCTGGTCGACGCGGGTTGTGATGGTCTGGTGCTCTCGGGCA
CCACCGGCGAGTCGCCGACCACTACTGACGACGAGAAACTCCAACTGTTGCGTGTCGTACTTGAGGCGGTAGGTGACCGA
GCTAGAGTCATCGCCGGCGCAGGTAGTTATGACACAGCTCATAGTGTCCGACTCGTCAAGGCCTGTGCGGGTGAGGGCGC
GCACGGACTTCTGGTGGTTACCCCTTACTACTCGAAGCCGCCGCAGACCGGGCTGTTTGCGCACTTCACCGCTGTGGCCG
ACGCGACTGAGCTACCAGTGTTGCTCTACGACATTCCCGGGCGGTCGGTCGTGCCGATCGAGCCTGACACGATTCGCGCG
CTGGCGTCGCATCCCAACATCGTCGGAGTCAAAGAGGCCAAGGCTGATTTATACAGCGGTGCCCGGATCATGGCTGACAC
CGGCCTGGCCTACTATTCCGGCGACGACGCACTGAACCTGCCCTGGCTGGCGGTGGGTGCCATCGGCTTCATCAGTGTGA
TTTCTCATCTAGCCGCAGGACAGCTTCGAGAGCTGTTATCCGCTTTTGGTTCTGGGGATATTACCACTGCCCGAAAGATC
AACGTCGCGATCGGCCCGCTGTGCAGCGCGATGGACCGCTTGGGTGGGGTGACGATGTCCAAGGCAGGTCTGCGGCTTCA
GGGTATCGACGTCGGTGATCCGCGGTTGCCGCAGATGCCGGCAACAGCGGAGCAGATCGATGAGTTGGCTGTCGATATGC
GTGCAGCCTCGGTGCTTAGGTGA

Upstream 100 bases:

>100_bases
CCAGCGTCCGTGGAGGTACAACTCAGGCGGCGTGTTGCTGGACAAACACGCGCCCTTGCGCGGTTGCTGAAAGCTTGGCA
TCGCCAGGTAACCTGTGGCC

Downstream 100 bases:

>100_bases
ACTAAAAGGTGGCTAACAAGTGGATGTAGCCCTGGCCCCGCCAGGTCCGCTGGCCTCAGGTGGGTTGAGGGTTACAGCGT
TGGGAGGCATCAGCGAAATC

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MTTVGFDVPARLGTLLTAMVTPFDADGSVDTAAATRLANRLVDAGCDGLVLSGTTGESPTTTDDEKLQLLRVVLEAVGDR
ARVIAGAGSYDTAHSVRLVKACAGEGAHGLLVVTPYYSKPPQTGLFAHFTAVADATELPVLLYDIPGRSVVPIEPDTIRA
LASHPNIVGVKEAKADLYSGARIMADTGLAYYSGDDALNLPWLAVGAIGFISVISHLAAGQLRELLSAFGSGDITTARKI
NVAIGPLCSAMDRLGGVTMSKAGLRLQGIDVGDPRLPQMPATAEQIDELAVDMRAASVLR

Sequences:

>Translated_300_residues
MTTVGFDVPARLGTLLTAMVTPFDADGSVDTAAATRLANRLVDAGCDGLVLSGTTGESPTTTDDEKLQLLRVVLEAVGDR
ARVIAGAGSYDTAHSVRLVKACAGEGAHGLLVVTPYYSKPPQTGLFAHFTAVADATELPVLLYDIPGRSVVPIEPDTIRA
LASHPNIVGVKEAKADLYSGARIMADTGLAYYSGDDALNLPWLAVGAIGFISVISHLAAGQLRELLSAFGSGDITTARKI
NVAIGPLCSAMDRLGGVTMSKAGLRLQGIDVGDPRLPQMPATAEQIDELAVDMRAASVLR
>Mature_299_residues
TTVGFDVPARLGTLLTAMVTPFDADGSVDTAAATRLANRLVDAGCDGLVLSGTTGESPTTTDDEKLQLLRVVLEAVGDRA
RVIAGAGSYDTAHSVRLVKACAGEGAHGLLVVTPYYSKPPQTGLFAHFTAVADATELPVLLYDIPGRSVVPIEPDTIRAL
ASHPNIVGVKEAKADLYSGARIMADTGLAYYSGDDALNLPWLAVGAIGFISVISHLAAGQLRELLSAFGSGDITTARKIN
VAIGPLCSAMDRLGGVTMSKAGLRLQGIDVGDPRLPQMPATAEQIDELAVDMRAASVLR

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=286, Percent_Identity=26.2237762237762, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI13540533, Length=300, Percent_Identity=26, Blast_Score=88, Evalue=8e-18,
Organism=Escherichia coli, GI1788823, Length=270, Percent_Identity=34.8148148148148, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789620, Length=285, Percent_Identity=27.3684210526316, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1786463, Length=234, Percent_Identity=30.3418803418803, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87082415, Length=287, Percent_Identity=27.8745644599303, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_MYCLB (B8ZRR3)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503690.1
- ProteinModelPortal:   B8ZRR3
- SMR:   B8ZRR3
- EnsemblBacteria:   EBMYCT00000086848
- GeneID:   7326927
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016215
- HOGENOM:   HBG358848
- OMA:   GMYRHFK
- ProtClustDB:   PRK03170
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 31137; Mature: 31006

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 171-171 BINDING 116-116

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVGFDVPARLGTLLTAMVTPFDADGSVDTAAATRLANRLVDAGCDGLVLSGTTGESPT
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
TTDDEKLQLLRVVLEAVGDRARVIAGAGSYDTAHSVRLVKACAGEGAHGLLVVTPYYSKP
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCC
PQTGLFAHFTAVADATELPVLLYDIPGRSVVPIEPDTIRALASHPNIVGVKEAKADLYSG
CCCCHHHHHHHHCCCCCCCEEEEECCCCEEECCCCHHHHHHHCCCCEEEHHHHHHHHHCC
ARIMADTGLAYYSGDDALNLPWLAVGAIGFISVISHLAAGQLRELLSAFGSGDITTARKI
CEEEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
NVAIGPLCSAMDRLGGVTMSKAGLRLQGIDVGDPRLPQMPATAEQIDELAVDMRAASVLR
EEHHHHHHHHHHHHCCCEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTVGFDVPARLGTLLTAMVTPFDADGSVDTAAATRLANRLVDAGCDGLVLSGTTGESPT
CCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
TTDDEKLQLLRVVLEAVGDRARVIAGAGSYDTAHSVRLVKACAGEGAHGLLVVTPYYSKP
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCC
PQTGLFAHFTAVADATELPVLLYDIPGRSVVPIEPDTIRALASHPNIVGVKEAKADLYSG
CCCCHHHHHHHHCCCCCCCEEEEECCCCEEECCCCHHHHHHHCCCCEEEHHHHHHHHHCC
ARIMADTGLAYYSGDDALNLPWLAVGAIGFISVISHLAAGQLRELLSAFGSGDITTARKI
CEEEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
NVAIGPLCSAMDRLGGVTMSKAGLRLQGIDVGDPRLPQMPATAEQIDELAVDMRAASVLR
EEHHHHHHHHHHHHCCCEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA