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The map label for this gene is glmU

Identifier: 221229523

GI number: 221229523

Start: 324572

End: 326050

Strand: Reverse

Name: glmU

Synonym: MLBr_00249

Alternate gene names: 221229523

Gene position: 326050-324572 (Counterclockwise)

Preceding gene: 221229530

Following gene: 221229522

Centisome position: 9.98

GC content: 60.18

Gene sequence:

>1479_bases
ATGACGTTTCGTGGTGATACCGCGGTCCTCGTCTTAGCGGCCGGCCCCGGCAGCCGAATGCGGTCAGATACACCGAAAGT
GCTGCACACCATCGCTGGTCGCAGCATGTTGTCGCATTCACTGCACGCGATCACCAAACTGGCACCCCAGCATCTAGTCG
TGGTACTAGGCCACGAGCATCAGCGCATTGCGCCGCTAGTCGCCGAACTGGCCGACACCCTCGAACGCACGATTGACGTC
GCATTACAGGATCGACCTCGCGGTACCGGACATGCCGTGTTCTGTGGCCTGTCCGCATTACCCGATGACTACGGCGGCAT
TGTCGTTGTAACTTCAGGCGATACCCCACTGCTCGACGCCAACACTCTGGCCGAACTGATCGCAACCCACAACGCCACAT
CCGCCGCAGTGACGGTGCTAACCACGACATTTAGCGATCCCCTTGGCTACGGCCGGATCCTGCGCACCCAGGACAATGAA
GTTATGGCGATTATCGAGCACGCCGACGCATCGCCTTCACAACGCGAGATCCGCGAGGTAAATGCCGGCGTGTATGCCTT
CGATATCACGGCGTTGCGGTCGGCGTTGATCAGACTGAACTCCAACAACACCCAACAGGAGCTCTACCTCACCGACGTGA
TCTCCATCCTTCGCAGGGAAGGCCAAAAGGTGAATGCTCAACACATCGACGACAACGCACTTGTGGCTGGCGTCAACAAC
CGCGTTCAGCTGGCCGAACTGAGCGCCGAACTCAACCGCCGCATTGTGGCCACTCACCAGGTGGCCGGCGTTACCATCAT
CGATCCTGCTACCACTTGGATCGATATAGACGTGACTATAGGCAACGACACCGTAATCCACCCCGGTACTCAGTTGCTCG
GCCGCACTCAGATCGGCGAGTGCTGCGTCATCGGCCCGGACACCACGCTGACCGACGTCTTGGTCAGTCAGCGTGCCACA
GTGGTCCGCACGCACGGCACATCGTCAACTATAGGTGCGGGCGCTATGGTCGGTCCGTTCACCTACCTGAGGCCCGGAAC
CGTGCTGGGCACCAAGGGTAAGTTGGGCGCCTTCGTCGAGACCAAAAACTCCACAATCGGAACCGGCGCCAAAGTGCCGC
ATTTGACCTACGTCGGCGACGCCGATATCGGCGAGCACAGCAACATCGGCGCGTCCAGCGTATTCGTCAACTACGATGGC
ACAGCCAAACAGCGCACCACTATCGGGTCACACGTACGCACTGGGTCGGACACCAAGTTCGTCGCGCCGGTGACTGTGGG
CGACGGCGCTTACACAGGCGCCGGCACAGTCGTGCGCAACGACGTCCCGCCGGGGGCACTGGCTGTTTCCGTGAGCCCCC
AACGCAACATCGAAAACTGGGTGCAGCGCAAGCGACCCGGCAGTGCCGCAGCTCAGGCAGCAGAAAAGGCCTCAACACGT
ACAGGCAAACAATCCCAGCAGAAATCCGAACCTGACTAG

Upstream 100 bases:

>100_bases
GAACGGCGTTTTTGCCGCAAGCGTGCATGAAATGCCACGCTTGACGGCGAGTCGACGTACAGACACGCGCGCTCACGGGC
AAACCACTCGAGGAGAGTCG

Downstream 100 bases:

>100_bases
CCACCGTGAATTTCGTTTTCCGGCAACCCGGCCACGTGTTCGGACCAAACACTTACATACGATGAGGCCTTCTCATTTCC
TATCTCAACCCCAGATACGG

Product: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MTFRGDTAVLVLAAGPGSRMRSDTPKVLHTIAGRSMLSHSLHAITKLAPQHLVVVLGHEHQRIAPLVAELADTLERTIDV
ALQDRPRGTGHAVFCGLSALPDDYGGIVVVTSGDTPLLDANTLAELIATHNATSAAVTVLTTTFSDPLGYGRILRTQDNE
VMAIIEHADASPSQREIREVNAGVYAFDITALRSALIRLNSNNTQQELYLTDVISILRREGQKVNAQHIDDNALVAGVNN
RVQLAELSAELNRRIVATHQVAGVTIIDPATTWIDIDVTIGNDTVIHPGTQLLGRTQIGECCVIGPDTTLTDVLVSQRAT
VVRTHGTSSTIGAGAMVGPFTYLRPGTVLGTKGKLGAFVETKNSTIGTGAKVPHLTYVGDADIGEHSNIGASSVFVNYDG
TAKQRTTIGSHVRTGSDTKFVAPVTVGDGAYTGAGTVVRNDVPPGALAVSVSPQRNIENWVQRKRPGSAAAQAAEKASTR
TGKQSQQKSEPD

Sequences:

>Translated_492_residues
MTFRGDTAVLVLAAGPGSRMRSDTPKVLHTIAGRSMLSHSLHAITKLAPQHLVVVLGHEHQRIAPLVAELADTLERTIDV
ALQDRPRGTGHAVFCGLSALPDDYGGIVVVTSGDTPLLDANTLAELIATHNATSAAVTVLTTTFSDPLGYGRILRTQDNE
VMAIIEHADASPSQREIREVNAGVYAFDITALRSALIRLNSNNTQQELYLTDVISILRREGQKVNAQHIDDNALVAGVNN
RVQLAELSAELNRRIVATHQVAGVTIIDPATTWIDIDVTIGNDTVIHPGTQLLGRTQIGECCVIGPDTTLTDVLVSQRAT
VVRTHGTSSTIGAGAMVGPFTYLRPGTVLGTKGKLGAFVETKNSTIGTGAKVPHLTYVGDADIGEHSNIGASSVFVNYDG
TAKQRTTIGSHVRTGSDTKFVAPVTVGDGAYTGAGTVVRNDVPPGALAVSVSPQRNIENWVQRKRPGSAAAQAAEKASTR
TGKQSQQKSEPD
>Mature_491_residues
TFRGDTAVLVLAAGPGSRMRSDTPKVLHTIAGRSMLSHSLHAITKLAPQHLVVVLGHEHQRIAPLVAELADTLERTIDVA
LQDRPRGTGHAVFCGLSALPDDYGGIVVVTSGDTPLLDANTLAELIATHNATSAAVTVLTTTFSDPLGYGRILRTQDNEV
MAIIEHADASPSQREIREVNAGVYAFDITALRSALIRLNSNNTQQELYLTDVISILRREGQKVNAQHIDDNALVAGVNNR
VQLAELSAELNRRIVATHQVAGVTIIDPATTWIDIDVTIGNDTVIHPGTQLLGRTQIGECCVIGPDTTLTDVLVSQRATV
VRTHGTSSTIGAGAMVGPFTYLRPGTVLGTKGKLGAFVETKNSTIGTGAKVPHLTYVGDADIGEHSNIGASSVFVNYDGT
AKQRTTIGSHVRTGSDTKFVAPVTVGDGAYTGAGTVVRNDVPPGALAVSVSPQRNIENWVQRKRPGSAAAQAAEKASTRT
GKQSQQKSEPD

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=467, Percent_Identity=37.4732334047109, Blast_Score=284, Evalue=1e-77,
Organism=Drosophila melanogaster, GI21355443, Length=402, Percent_Identity=21.8905472636816, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24644084, Length=402, Percent_Identity=21.8905472636816, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_MYCLB (B8ZU67)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002502939.1
- ProteinModelPortal:   B8ZU67
- SMR:   B8ZU67
- EnsemblBacteria:   EBMYCT00000086197
- GeneID:   7325018
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016064
- HOGENOM:   HBG688195
- OMA:   GSKVNHL
- ProtClustDB:   PRK14352
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 51958; Mature: 51827

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 374-374 BINDING 83-83 BINDING 151-151 BINDING 166-166 BINDING 181-181 BINDING 398-398 BINDING 416-416 BINDING 434-434

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFRGDTAVLVLAAGPGSRMRSDTPKVLHTIAGRSMLSHSLHAITKLAPQHLVVVLGHEH
CCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
QRIAPLVAELADTLERTIDVALQDRPRGTGHAVFCGLSALPDDYGGIVVVTSGDTPLLDA
HHHHHHHHHHHHHHHHHHEEHCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCEECH
NTLAELIATHNATSAAVTVLTTTFSDPLGYGRILRTQDNEVMAIIEHADASPSQREIREV
HHHHHHHHHCCCCCEEEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHH
NAGVYAFDITALRSALIRLNSNNTQQELYLTDVISILRREGQKVNAQHIDDNALVAGVNN
CCCEEEEEHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEECCCC
RVQLAELSAELNRRIVATHQVAGVTIIDPATTWIDIDVTIGNDTVIHPGTQLLGRTQIGE
CEEHHHHHHHHCCEEEEEEEECCEEEECCCCEEEEEEEEECCCEEECCCHHHHCCCCCCC
CCVIGPDTTLTDVLVSQRATVVRTHGTSSTIGAGAMVGPFTYLRPGTVLGTKGKLGAFVE
EEEECCCCHHHHHHHHCCCEEEEECCCCCCCCCCCEECCEEEECCCEEEECCCCCCEEEE
TKNSTIGTGAKVPHLTYVGDADIGEHSNIGASSVFVNYDGTAKQRTTIGSHVRTGSDTKF
ECCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHCCCCEECCCCCEE
VAPVTVGDGAYTGAGTVVRNDVPPGALAVSVSPQRNIENWVQRKRPGSAAAQAAEKASTR
EEEEEECCCCCCCCCCEEECCCCCCEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHC
TGKQSQQKSEPD
CCCHHHHCCCCC
>Mature Secondary Structure 
TFRGDTAVLVLAAGPGSRMRSDTPKVLHTIAGRSMLSHSLHAITKLAPQHLVVVLGHEH
CCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
QRIAPLVAELADTLERTIDVALQDRPRGTGHAVFCGLSALPDDYGGIVVVTSGDTPLLDA
HHHHHHHHHHHHHHHHHHEEHCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCEECH
NTLAELIATHNATSAAVTVLTTTFSDPLGYGRILRTQDNEVMAIIEHADASPSQREIREV
HHHHHHHHHCCCCCEEEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHH
NAGVYAFDITALRSALIRLNSNNTQQELYLTDVISILRREGQKVNAQHIDDNALVAGVNN
CCCEEEEEHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEECCCC
RVQLAELSAELNRRIVATHQVAGVTIIDPATTWIDIDVTIGNDTVIHPGTQLLGRTQIGE
CEEHHHHHHHHCCEEEEEEEECCEEEECCCCEEEEEEEEECCCEEECCCHHHHCCCCCCC
CCVIGPDTTLTDVLVSQRATVVRTHGTSSTIGAGAMVGPFTYLRPGTVLGTKGKLGAFVE
EEEECCCCHHHHHHHHCCCEEEEECCCCCCCCCCCEECCEEEECCCEEEECCCCCCEEEE
TKNSTIGTGAKVPHLTYVGDADIGEHSNIGASSVFVNYDGTAKQRTTIGSHVRTGSDTKF
ECCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHCCCCEECCCCCEE
VAPVTVGDGAYTGAGTVVRNDVPPGALAVSVSPQRNIENWVQRKRPGSAAAQAAEKASTR
EEEEEECCCCCCCCCCEEECCCCCCEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHC
TGKQSQQKSEPD
CCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA