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The map label for this gene is prsA

Identifier: 221229522

GI number: 221229522

Start: 323478

End: 324461

Strand: Reverse

Name: prsA

Synonym: MLBr_00248

Alternate gene names: 221229522

Gene position: 324461-323478 (Counterclockwise)

Preceding gene: 221229523

Following gene: 221229521

Centisome position: 9.93

GC content: 60.47

Gene sequence:

>984_bases
ATGTTGAGCCACGACTGGACCGACAATCGAAAAAACCTGATGCTCTTTTCGGGCCGTGCGCATCCCGAGCTAGCCGAGCA
GGTAGCCAAAGAACTCGACATGCACGTCACCACCCAGGACGCGCGGGAATTCGCCAACGGCGAGATCTTCGTGCGCTTCC
ACGAATCAGTGCGCGGCTGTGACGCCTTCGTCCTGCAATCTTGCCCGGCACCGGTGAATACCTGGCTGATGGAACAGCTG
ATCATGATCGACGCGCTCAAGCGGGGCAGTGCCAAGCGGATCACCGCCGTCATACCATTCTACCCTTACGCTCGACAGGA
CAAGAAGCACCGCGGCCGCGAGCCCATCTCGGCGCGACTGGTCGCTGACTTGCTTAAAACGGCGGGCGCCGACCGGATCG
TGACGGTCGACCTACACACCGACCAGATTCAGGGTTTCTTTGACGGGCCCGTCGACCACATGCGCGGACAAAACCTGTTG
ACGGGGTATATCAAGAACAATTACCCGGACACCAACATGGTGGTGGTCTCCCCCGACTCCGGCAGAGTGCGGATCGCCGA
GAAATGGGGCGACGCACTGGGCGGTGTCCCGCTGGCCTTCATTCACAAGACCCGCGACCTTCGCGTACCCAACCAAGTGG
TATCCAACCGAGTTGTCGGCGAAGTCGAAGGTCGCACCTGTGTGCTGATCGACGACATGATCGACACTGGCGGCACCGTC
GCCGGCGCAGTGCAATTGCTGCGTAACGATGGCGCCAGTGACGTAATCATCGCAGCTACCCACGGTGTGCTGTCGCCCCC
GGCTGCTGAACGGCTGGCCCAGTACGGCACCCGGGAAGTGATCGTCACGAACACGCTACCGATCGGCGAAGAGAAGCGTT
TCCCCCAGCTCACGGTTTTGTCCATCGCACCGTTGCTAGCCAGCACCATCCGCGCCATTTTCGAAAATGGCTCAGTCACA
GGGCTATTCGACGGAGAAGCGTAG

Upstream 100 bases:

>100_bases
TTTCGTTTTCCGGCAACCCGGCCACGTGTTCGGACCAAACACTTACATACGATGAGGCCTTCTCATTTCCTATCTCAACC
CCAGATACGGCGAGGGCGGC

Downstream 100 bases:

>100_bases
ATGGTTACCGGACAAAACTATCCTTCGGGGCACCATCGTCATCTACCACAACCCGATGTACAGCACCTCTTGTAAGATGC
TAAACTTAGTGCGAAAGAAA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MLSHDWTDNRKNLMLFSGRAHPELAEQVAKELDMHVTTQDAREFANGEIFVRFHESVRGCDAFVLQSCPAPVNTWLMEQL
IMIDALKRGSAKRITAVIPFYPYARQDKKHRGREPISARLVADLLKTAGADRIVTVDLHTDQIQGFFDGPVDHMRGQNLL
TGYIKNNYPDTNMVVVSPDSGRVRIAEKWGDALGGVPLAFIHKTRDLRVPNQVVSNRVVGEVEGRTCVLIDDMIDTGGTV
AGAVQLLRNDGASDVIIAATHGVLSPPAAERLAQYGTREVIVTNTLPIGEEKRFPQLTVLSIAPLLASTIRAIFENGSVT
GLFDGEA

Sequences:

>Translated_327_residues
MLSHDWTDNRKNLMLFSGRAHPELAEQVAKELDMHVTTQDAREFANGEIFVRFHESVRGCDAFVLQSCPAPVNTWLMEQL
IMIDALKRGSAKRITAVIPFYPYARQDKKHRGREPISARLVADLLKTAGADRIVTVDLHTDQIQGFFDGPVDHMRGQNLL
TGYIKNNYPDTNMVVVSPDSGRVRIAEKWGDALGGVPLAFIHKTRDLRVPNQVVSNRVVGEVEGRTCVLIDDMIDTGGTV
AGAVQLLRNDGASDVIIAATHGVLSPPAAERLAQYGTREVIVTNTLPIGEEKRFPQLTVLSIAPLLASTIRAIFENGSVT
GLFDGEA
>Mature_327_residues
MLSHDWTDNRKNLMLFSGRAHPELAEQVAKELDMHVTTQDAREFANGEIFVRFHESVRGCDAFVLQSCPAPVNTWLMEQL
IMIDALKRGSAKRITAVIPFYPYARQDKKHRGREPISARLVADLLKTAGADRIVTVDLHTDQIQGFFDGPVDHMRGQNLL
TGYIKNNYPDTNMVVVSPDSGRVRIAEKWGDALGGVPLAFIHKTRDLRVPNQVVSNRVVGEVEGRTCVLIDDMIDTGGTV
AGAVQLLRNDGASDVIIAATHGVLSPPAAERLAQYGTREVIVTNTLPIGEEKRFPQLTVLSIAPLLASTIRAIFENGSVT
GLFDGEA

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family

Homologues:

Organism=Homo sapiens, GI28557709, Length=314, Percent_Identity=42.6751592356688, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI4506129, Length=314, Percent_Identity=42.6751592356688, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI4506127, Length=314, Percent_Identity=42.3566878980892, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI84875539, Length=317, Percent_Identity=42.2712933753943, Blast_Score=237, Evalue=9e-63,
Organism=Homo sapiens, GI4506133, Length=344, Percent_Identity=32.8488372093023, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI194018537, Length=339, Percent_Identity=34.2182890855457, Blast_Score=164, Evalue=9e-41,
Organism=Homo sapiens, GI310128524, Length=142, Percent_Identity=31.6901408450704, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI310115209, Length=142, Percent_Identity=31.6901408450704, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI310118259, Length=142, Percent_Identity=31.6901408450704, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI310119946, Length=142, Percent_Identity=31.6901408450704, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1787458, Length=316, Percent_Identity=43.6708860759494, Blast_Score=245, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI25149168, Length=314, Percent_Identity=42.6751592356688, Blast_Score=247, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI17554702, Length=314, Percent_Identity=42.6751592356688, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI71989924, Length=314, Percent_Identity=42.6751592356688, Blast_Score=244, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI17554704, Length=311, Percent_Identity=42.7652733118971, Blast_Score=244, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI17570245, Length=346, Percent_Identity=33.5260115606936, Blast_Score=184, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6321776, Length=314, Percent_Identity=41.4012738853503, Blast_Score=233, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6319403, Length=315, Percent_Identity=39.6825396825397, Blast_Score=229, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6320946, Length=314, Percent_Identity=39.4904458598726, Blast_Score=228, Evalue=8e-61,
Organism=Saccharomyces cerevisiae, GI6322667, Length=207, Percent_Identity=39.6135265700483, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6324511, Length=95, Percent_Identity=41.0526315789474, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21355239, Length=314, Percent_Identity=42.3566878980892, Blast_Score=245, Evalue=3e-65,
Organism=Drosophila melanogaster, GI45551540, Length=337, Percent_Identity=39.7626112759644, Blast_Score=234, Evalue=8e-62,
Organism=Drosophila melanogaster, GI24651458, Length=355, Percent_Identity=31.830985915493, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24651456, Length=355, Percent_Identity=31.830985915493, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI281362873, Length=355, Percent_Identity=31.830985915493, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24651454, Length=355, Percent_Identity=31.830985915493, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24651462, Length=374, Percent_Identity=29.6791443850267, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24651464, Length=374, Percent_Identity=29.6791443850267, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI45552010, Length=374, Percent_Identity=29.6791443850267, Blast_Score=139, Evalue=2e-33,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KPRS_MYCLE (Q9CD45)

Other databases:

- EMBL:   AL583917
- PIR:   H86939
- RefSeq:   NP_301307.1
- ProteinModelPortal:   Q9CD45
- SMR:   Q9CD45
- EnsemblBacteria:   EBMYCT00000028926
- GeneID:   908729
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0248
- NMPDR:   fig|272631.1.peg.179
- Leproma:   ML0248
- GeneTree:   EBGT00050000016214
- HOGENOM:   HBG519284
- OMA:   YKTAGAD
- ProtClustDB:   PRK03092
- BioCyc:   MLEP272631:ML0248-MONOMER
- BRENDA:   2.7.6.1
- GO:   GO:0005737
- HAMAP:   MF_00583_B
- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01251

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.7.6.1

Molecular weight: Translated: 35904; Mature: 35904

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00114 PRPP_SYNTHASE; PS00103 PUR_PYR_PR_TRANSFER ; PS00144 ASN_GLN_ASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSHDWTDNRKNLMLFSGRAHPELAEQVAKELDMHVTTQDAREFANGEIFVRFHESVRGC
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCEEECHHHHHHCCCEEEEEEECHHCCH
DAFVLQSCPAPVNTWLMEQLIMIDALKRGSAKRITAVIPFYPYARQDKKHRGREPISARL
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCHHHHH
VADLLKTAGADRIVTVDLHTDQIQGFFDGPVDHMRGQNLLTGYIKNNYPDTNMVVVSPDS
HHHHHHHCCCCEEEEEEEEHHHHCCCCCCCHHHHCCCCEEEEEECCCCCCCCEEEECCCC
GRVRIAEKWGDALGGVPLAFIHKTRDLRVPNQVVSNRVVGEVEGRTCVLIDDMIDTGGTV
CCEEEHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCEEEECCCEEEEEECCCCCCCHH
AGAVQLLRNDGASDVIIAATHGVLSPPAAERLAQYGTREVIVTNTLPIGEEKRFPQLTVL
HHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHH
SIAPLLASTIRAIFENGSVTGLFDGEA
HHHHHHHHHHHHHHCCCCEEEEECCCC
>Mature Secondary Structure
MLSHDWTDNRKNLMLFSGRAHPELAEQVAKELDMHVTTQDAREFANGEIFVRFHESVRGC
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCEEECHHHHHHCCCEEEEEEECHHCCH
DAFVLQSCPAPVNTWLMEQLIMIDALKRGSAKRITAVIPFYPYARQDKKHRGREPISARL
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCHHHHH
VADLLKTAGADRIVTVDLHTDQIQGFFDGPVDHMRGQNLLTGYIKNNYPDTNMVVVSPDS
HHHHHHHCCCCEEEEEEEEHHHHCCCCCCCHHHHCCCCEEEEEECCCCCCCCEEEECCCC
GRVRIAEKWGDALGGVPLAFIHKTRDLRVPNQVVSNRVVGEVEGRTCVLIDDMIDTGGTV
CCEEEHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCEEEECCCEEEEEECCCCCCCHH
AGAVQLLRNDGASDVIIAATHGVLSPPAAERLAQYGTREVIVTNTLPIGEEKRFPQLTVL
HHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHH
SIAPLLASTIRAIFENGSVTGLFDGEA
HHHHHHHHHHHHHHCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002