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The map label for this gene is ksgA

Identifier: 221229515

GI number: 221229515

Start: 316064

End: 316984

Strand: Direct

Name: ksgA

Synonym: MLBr_00241

Alternate gene names: 221229515

Gene position: 316064-316984 (Clockwise)

Preceding gene: 221229514

Following gene: 221229516

Centisome position: 9.67

GC content: 59.07

Gene sequence:

>921_bases
GTGCAGCGGAAGAGTGGGTGCACACTGACCATCCGGCTACTCGAGCGTACCGAGATCAGGTGGCTGGTAAAAGAACTTGA
ATGTCGGCCACGGAAATCTCTCGGCCAAAATTTCGTGCACGACGCCAACACGGTGCGACGTGTGGTTTCGACTTCCAGAG
TGAACCGGTCTGACTTCGTCTTGGAGGTTGGTCCTGGCTTCGGATCGCTCACGCTGGCATTGCTGGACTGCGGCGCTGCC
GTCAGTGCCATAGAAATTGACCCAGTGTTAGCGGGTCGGTTGCCGCAGACGGTTGCTGAGCATTCAAATAATGAGATCCA
CCGGCTCACGGTGTGCAACCGCGACGTTTTATCCTTTCGCCGTGGCGATCTGGCTACCGAGCCGACGGCGCTAGTTGCCA
ACCTGCCGTACAACGTTGCGGTTCCGGCGTTATTGCATCTGCTCGCCGAGTTCCCGTCCATCAGGACTGTTACGGTGATG
GTGCAGGCTGAGGTCGCCGAACGGCTTGCCGCCGAGCCGGGTGGCAAGGATTACGGCGTGCCCAGCGTCAAGTTGAGTTT
CTTTGGACGGGTCCGTCGCTGTGGCATGGTGTCTCCGACTGTTTTCTGGCCGATTCCTCGTGTCTACTCCGGTTTGGTAC
GCGTCGACCGCTATGCGACTTCGCCATGGCCGACTGATGACGCCTTCCGTCGGCAGGTATTCGAACTGGTAGACATTGCA
TTCACTCAGCGGCGCAAGACATCTCGCAACGCGTTTGTGAAGTGGGCGGGTTCGAGTAACGAATCAGCGAATCGATTGCT
TGCCGCTAGCATCGATCCTGCCCGTCGCGGCGAGACGCTGTCCATCGACGACTTCGTTCGACTGCTGCGACGTTCTGATG
GTCGAGACGACGCGGCGGTGCGGTCTGCTTCGGCGAGCTGA

Upstream 100 bases:

>100_bases
GCTTGCGGTATGCTCCCCGTGCTGACCTTGCCACCCGAGAAGAGCAGATCGCGGTTGCTGAGGTGACGCGGGCGCGCCAA
GGATGGGATGCTTGGCCAGT

Downstream 100 bases:

>100_bases
CGGGCGGGTTGGTTGCCTGCAGCGATATTCTGCTGTGGTGTTCGCGTCTGATGGTAACGCCGCTACACAGTGGATGCCCA
CCGGGTCGGTCACCGTTCAG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MQRKSGCTLTIRLLERTEIRWLVKELECRPRKSLGQNFVHDANTVRRVVSTSRVNRSDFVLEVGPGFGSLTLALLDCGAA
VSAIEIDPVLAGRLPQTVAEHSNNEIHRLTVCNRDVLSFRRGDLATEPTALVANLPYNVAVPALLHLLAEFPSIRTVTVM
VQAEVAERLAAEPGGKDYGVPSVKLSFFGRVRRCGMVSPTVFWPIPRVYSGLVRVDRYATSPWPTDDAFRRQVFELVDIA
FTQRRKTSRNAFVKWAGSSNESANRLLAASIDPARRGETLSIDDFVRLLRRSDGRDDAAVRSASAS

Sequences:

>Translated_306_residues
MQRKSGCTLTIRLLERTEIRWLVKELECRPRKSLGQNFVHDANTVRRVVSTSRVNRSDFVLEVGPGFGSLTLALLDCGAA
VSAIEIDPVLAGRLPQTVAEHSNNEIHRLTVCNRDVLSFRRGDLATEPTALVANLPYNVAVPALLHLLAEFPSIRTVTVM
VQAEVAERLAAEPGGKDYGVPSVKLSFFGRVRRCGMVSPTVFWPIPRVYSGLVRVDRYATSPWPTDDAFRRQVFELVDIA
FTQRRKTSRNAFVKWAGSSNESANRLLAASIDPARRGETLSIDDFVRLLRRSDGRDDAAVRSASAS
>Mature_306_residues
MQRKSGCTLTIRLLERTEIRWLVKELECRPRKSLGQNFVHDANTVRRVVSTSRVNRSDFVLEVGPGFGSLTLALLDCGAA
VSAIEIDPVLAGRLPQTVAEHSNNEIHRLTVCNRDVLSFRRGDLATEPTALVANLPYNVAVPALLHLLAEFPSIRTVTVM
VQAEVAERLAAEPGGKDYGVPSVKLSFFGRVRRCGMVSPTVFWPIPRVYSGLVRVDRYATSPWPTDDAFRRQVFELVDIA
FTQRRKTSRNAFVKWAGSSNESANRLLAASIDPARRGETLSIDDFVRLLRRSDGRDDAAVRSASAS

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=269, Percent_Identity=31.5985130111524, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI156415992, Length=307, Percent_Identity=28.9902280130293, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1786236, Length=266, Percent_Identity=32.7067669172932, Blast_Score=134, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI25146882, Length=181, Percent_Identity=34.8066298342541, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI25141369, Length=320, Percent_Identity=25.9375, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324989, Length=223, Percent_Identity=29.5964125560538, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21358017, Length=278, Percent_Identity=28.7769784172662, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_MYCLB (B8ZU59)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002502931.1
- ProteinModelPortal:   B8ZU59
- SMR:   B8ZU59
- EnsemblBacteria:   EBMYCT00000085208
- GeneID:   7326327
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000014608
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- ProtClustDB:   PRK00274
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 33917; Mature: 33917

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 37-37 BINDING 39-39 BINDING 64-64 BINDING 85-85 BINDING 115-115 BINDING 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRKSGCTLTIRLLERTEIRWLVKELECRPRKSLGQNFVHDANTVRRVVSTSRVNRSDFV
CCCCCCCEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LEVGPGFGSLTLALLDCGAAVSAIEIDPVLAGRLPQTVAEHSNNEIHRLTVCNRDVLSFR
EEECCCCCHHHHHHHHHCCHHEEEEECHHHHCCCHHHHHHCCCCCEEEEEECCHHHHHHH
RGDLATEPTALVANLPYNVAVPALLHLLAEFPSIRTVTVMVQAEVAERLAAEPGGKDYGV
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHCCCCCCCCCC
PSVKLSFFGRVRRCGMVSPTVFWPIPRVYSGLVRVDRYATSPWPTDDAFRRQVFELVDIA
CCCHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
FTQRRKTSRNAFVKWAGSSNESANRLLAASIDPARRGETLSIDDFVRLLRRSDGRDDAAV
HHHHHHCCCCEEEEECCCCCCCCCEEEEECCCHHHCCCEECHHHHHHHHHHCCCCCHHHH
RSASAS
HCCCCC
>Mature Secondary Structure
MQRKSGCTLTIRLLERTEIRWLVKELECRPRKSLGQNFVHDANTVRRVVSTSRVNRSDFV
CCCCCCCEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LEVGPGFGSLTLALLDCGAAVSAIEIDPVLAGRLPQTVAEHSNNEIHRLTVCNRDVLSFR
EEECCCCCHHHHHHHHHCCHHEEEEECHHHHCCCHHHHHHCCCCCEEEEEECCHHHHHHH
RGDLATEPTALVANLPYNVAVPALLHLLAEFPSIRTVTVMVQAEVAERLAAEPGGKDYGV
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHCCCCCCCCCC
PSVKLSFFGRVRRCGMVSPTVFWPIPRVYSGLVRVDRYATSPWPTDDAFRRQVFELVDIA
CCCHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
FTQRRKTSRNAFVKWAGSSNESANRLLAASIDPARRGETLSIDDFVRLLRRSDGRDDAAV
HHHHHHCCCCEEEEECCCCCCCCCEEEEECCCHHHCCCEECHHHHHHHHHHCCCCCHHHH
RSASAS
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA