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The map label for this gene is ispE

Identifier: 221229516

GI number: 221229516

Start: 317058

End: 317993

Strand: Direct

Name: ispE

Synonym: MLBr_00242

Alternate gene names: 221229516

Gene position: 317058-317993 (Clockwise)

Preceding gene: 221229515

Following gene: 221229517

Centisome position: 9.7

GC content: 64.64

Gene sequence:

>936_bases
ATGCCCACCGGGTCGGTCACCGTTCAGGTTCCCGGGAAGATCAATCTCTACCTCGCTGTCGGTGATTGCTGCGATAATGG
CTACCACGAGCTGGTGACGGTTTTCCATGCTGTGTCGCTGGTCGACCAGGTTACGGTGCGCAACGCCGACGTGCTGTCGC
TCGGACTTGTCGGCGAGGGCGCCAACCACGTGCCCACTGACGAACACAACATTGCCTGGCGGGCGGCCGAGCTAATGGCC
GAGCACGTTGGCCGGGCTCCGGACGTTTCGATCATGATTGACAAGTCCATTCCGGTGGCTGGGGGGATGGCGGGCGGCAG
CGCCGATGCGGCCGCGGTGTTGGTTGCGATGAACTCGTTGTGGGAACTCAGCCTGCCTCGACGCGATCTGTGCATGCTCG
CAGCGAAGCTAGGCAGCGACGTGCCGTTCGCACTACACGGTGGTACCGCGCTGGGGACCGGTCGCGGTGAGGAGCTAGCT
ACCGTGTTGTCCCGCGCCACTTTCCACTGGGTGTTGGCGTTCGCCGACAGCAGTTTGCTCACTCCGGCGGTGTACACCGA
GTTTGACCGGCTCAGGGATGTCGGAAATCCACCACGGCTTGCCGAACCCGGGCCGGTGTTGGCCGCCCTGGTCGCCGCCG
ATCCCGAACAGTTGGCGCCGTTGCTAGGCAATGAATTGCAAGCGGCCGCGGTCAGCTTGGACCCGGCGCTACGGTGTGCG
CTCCGCGCCGGTATGGAAGCGGGTGCGCTCGCGGGCATTGTGTCTGGCTCGGGTCCTACCTGCGCATTCCTGTGTGCCTC
GGCGACTTCGGCGATCGATGTCGGTGCGCAGTTGGCAGGGGCGGGTGTCTGTCGCACTGTTCGGGTCGCCACCGGACCAG
TACCCGGTGCTCGGGTGGTACATGCGCCCATGAGCCGAGGTCTGAATGACATGTGA

Upstream 100 bases:

>100_bases
GCGGTGCGGTCTGCTTCGGCGAGCTGACGGGCGGGTTGGTTGCCTGCAGCGATATTCTGCTGTGGTGTTCGCGTCTGATG
GTAACGCCGCTACACAGTGG

Downstream 100 bases:

>100_bases
AATCTAACTCAAATTTACGTTATCGTTTGGCAGTGACTTAAGAGGAGTTTAAGATTCCTTGCGGTGACGTCGACGTTGTG
TCGGTGGGCGATTAACGAAT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MPTGSVTVQVPGKINLYLAVGDCCDNGYHELVTVFHAVSLVDQVTVRNADVLSLGLVGEGANHVPTDEHNIAWRAAELMA
EHVGRAPDVSIMIDKSIPVAGGMAGGSADAAAVLVAMNSLWELSLPRRDLCMLAAKLGSDVPFALHGGTALGTGRGEELA
TVLSRATFHWVLAFADSSLLTPAVYTEFDRLRDVGNPPRLAEPGPVLAALVAADPEQLAPLLGNELQAAAVSLDPALRCA
LRAGMEAGALAGIVSGSGPTCAFLCASATSAIDVGAQLAGAGVCRTVRVATGPVPGARVVHAPMSRGLNDM

Sequences:

>Translated_311_residues
MPTGSVTVQVPGKINLYLAVGDCCDNGYHELVTVFHAVSLVDQVTVRNADVLSLGLVGEGANHVPTDEHNIAWRAAELMA
EHVGRAPDVSIMIDKSIPVAGGMAGGSADAAAVLVAMNSLWELSLPRRDLCMLAAKLGSDVPFALHGGTALGTGRGEELA
TVLSRATFHWVLAFADSSLLTPAVYTEFDRLRDVGNPPRLAEPGPVLAALVAADPEQLAPLLGNELQAAAVSLDPALRCA
LRAGMEAGALAGIVSGSGPTCAFLCASATSAIDVGAQLAGAGVCRTVRVATGPVPGARVVHAPMSRGLNDM
>Mature_310_residues
PTGSVTVQVPGKINLYLAVGDCCDNGYHELVTVFHAVSLVDQVTVRNADVLSLGLVGEGANHVPTDEHNIAWRAAELMAE
HVGRAPDVSIMIDKSIPVAGGMAGGSADAAAVLVAMNSLWELSLPRRDLCMLAAKLGSDVPFALHGGTALGTGRGEELAT
VLSRATFHWVLAFADSSLLTPAVYTEFDRLRDVGNPPRLAEPGPVLAALVAADPEQLAPLLGNELQAAAVSLDPALRCAL
RAGMEAGALAGIVSGSGPTCAFLCASATSAIDVGAQLAGAGVCRTVRVATGPVPGARVVHAPMSRGLNDM

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=185, Percent_Identity=34.5945945945946, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1786184, Length=258, Percent_Identity=26.7441860465116, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_MYCLB (B8ZU60)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002502932.1
- ProteinModelPortal:   B8ZU60
- SMR:   B8ZU60
- EnsemblBacteria:   EBMYCT00000086928
- GeneID:   7324725
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016146
- HOGENOM:   HBG734593
- OMA:   RIYNGLR
- ProtClustDB:   PRK03188
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31794; Mature: 31663

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: ACT_SITE 13-13 ACT_SITE 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTGSVTVQVPGKINLYLAVGDCCDNGYHELVTVFHAVSLVDQVTVRNADVLSLGLVGEG
CCCCCEEEEECCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
ANHVPTDEHNIAWRAAELMAEHVGRAPDVSIMIDKSIPVAGGMAGGSADAAAVLVAMNSL
CCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
WELSLPRRDLCMLAAKLGSDVPFALHGGTALGTGRGEELATVLSRATFHWVLAFADSSLL
HHCCCCHHHHHHHHHHHCCCCCEEEECCCEECCCCCHHHHHHHHHHHHHHHEEECCCCCC
TPAVYTEFDRLRDVGNPPRLAEPGPVLAALVAADPEQLAPLLGNELQAAAVSLDPALRCA
CHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCHHHHHCCCCHHHHHH
LRAGMEAGALAGIVSGSGPTCAFLCASATSAIDVGAQLAGAGVCRTVRVATGPVPGARVV
HHCCCCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEE
HAPMSRGLNDM
ECHHHCCCCCC
>Mature Secondary Structure 
PTGSVTVQVPGKINLYLAVGDCCDNGYHELVTVFHAVSLVDQVTVRNADVLSLGLVGEG
CCCCEEEEECCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
ANHVPTDEHNIAWRAAELMAEHVGRAPDVSIMIDKSIPVAGGMAGGSADAAAVLVAMNSL
CCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
WELSLPRRDLCMLAAKLGSDVPFALHGGTALGTGRGEELATVLSRATFHWVLAFADSSLL
HHCCCCHHHHHHHHHHHCCCCCEEEECCCEECCCCCHHHHHHHHHHHHHHHEEECCCCCC
TPAVYTEFDRLRDVGNPPRLAEPGPVLAALVAADPEQLAPLLGNELQAAAVSLDPALRCA
CHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCHHHHHCCCCHHHHHH
LRAGMEAGALAGIVSGSGPTCAFLCASATSAIDVGAQLAGAGVCRTVRVATGPVPGARVV
HHCCCCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEE
HAPMSRGLNDM
ECHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA