Click here to switch to the map view.

The map label for this gene is rlmL [H]

Identifier: 220919233

GI number: 220919233

Start: 4639906

End: 4641069

Strand: Direct

Name: rlmL [H]

Synonym: A2cp1_4154

Alternate gene names: 220919233

Gene position: 4639906-4641069 (Clockwise)

Preceding gene: 220919232

Following gene: 220919234

Centisome position: 92.26

GC content: 76.89

Gene sequence:

>1164_bases
ATGCGCTTCTTCGCCACCTGCGCCAAGGGCACCGAGGGTGCCCTGCGGCGCGAGCTGTCCGGCCTCCGCCTCCCCGCCGT
CCGCGGCGATCGCGGCGGCGTCTCGTTCGAGGGGGCGCTGGAGGCCGGCATGCGGGCCTGCCTGCACGCGCGGACCGCCA
TGCGCGTCCTGCTGGAGCTGGCGCGCTTCCCGGCCCCGGACGCGGCGGCGCTGTACGAGGGCGCGCGCGCGGTCGCGTGG
CAGGACTGGCTCGACGTGCGGACCACGCTGGCGGTGGAGGCGACCGTCTCCTCGACCACCATCACGCACTCCGGCTTCGC
GGCGCTCAAGGTGAAGGACGCGGCGGTGGACGTGCTGCGCGAGCGGCTCGGCGCGCGCCCGGACGTGGACCCGAAGGACC
CGGACGTGCGGATCGTGCTGCACCTCGCCCGCGACGAGGCCACGCTGTCGCTCGATCTCGCCGGCGCGCCGCTGCACCGG
CGCGGCTACCGCGCGGTGACCACCGAGGCACCGCTCAAGGAGACGCTGGCCGCGGCGGTGCTGCTGCTCGGCGGCGTCGA
CCCGGCGCTCCCCTTCGCCGACCCGATGGCCGGCTCCGGGACGCTCGCCATCGAGCACGCGCTGCGCGCCCGGCGCATCG
CGCCCGGCCTGGACCGCGCGTTCGGCTTCCAGCGCTGGCCGAGCTACCGGGGCGGCCCGCAGTCGGCCTGGGACCGCATG
AAGGCGGCGGCGCGCGCCGAGATCCTGCCGCGGGCGCCGGCGCCGATCGTGGCGCGCGACGGGCACCCGAAGGCGATCCA
GGCGCTCCGGCTGAACGCCGCCGCGGCGGGCGTGGAGGCCGACCTCGAGATCTCGCAGGCCGACGCGCGCGACCTCCAGC
CGTCCGCGGACGCGGGCGCCATCGTGACCAACCCGCCCTACGGCGAGCGCCTGGGCGGCGGCGGCGAGGGCGCGAGCGCG
GCCGAGGCGCGCGTCGCGGGCAAGAAGCTGCAGGGCTTCTACCGCGGCCTGGCCGAGATGCTGGTGCGCCACCACGGCTG
GACCGCGGTGGTGCTCTCCGGCAACCCGCTCCTCGAGCGGGCCATCCCGATCCGGCCCGCGGTGGATCACCGGCTCTGGA
ACGGACCCCTCGAGGCCCACCTGCTGAAGTACGAGCTCCCGTGA

Upstream 100 bases:

>100_bases
ACGGCGCCAAGCGGCACGCCAAGGGCTCGATCCGCCTGGACGTGTTCGGGGGGATCGCGCCGGTGGCCGCGGACGGGACC
GATCCGCGGTAGACAGGGGC

Downstream 100 bases:

>100_bases
CCTCCTTGCGGAGCGCCGGACGCCGCGCCTAACGTTCCCGCGTGGCCGCCCCCGCCCCGCTCCTCGCCCTCGCGCTGGCG
CTCGCCGCCGGCGGCCCGGG

Product: RNA methylase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MRFFATCAKGTEGALRRELSGLRLPAVRGDRGGVSFEGALEAGMRACLHARTAMRVLLELARFPAPDAAALYEGARAVAW
QDWLDVRTTLAVEATVSSTTITHSGFAALKVKDAAVDVLRERLGARPDVDPKDPDVRIVLHLARDEATLSLDLAGAPLHR
RGYRAVTTEAPLKETLAAAVLLLGGVDPALPFADPMAGSGTLAIEHALRARRIAPGLDRAFGFQRWPSYRGGPQSAWDRM
KAAARAEILPRAPAPIVARDGHPKAIQALRLNAAAAGVEADLEISQADARDLQPSADAGAIVTNPPYGERLGGGGEGASA
AEARVAGKKLQGFYRGLAEMLVRHHGWTAVVLSGNPLLERAIPIRPAVDHRLWNGPLEAHLLKYELP

Sequences:

>Translated_387_residues
MRFFATCAKGTEGALRRELSGLRLPAVRGDRGGVSFEGALEAGMRACLHARTAMRVLLELARFPAPDAAALYEGARAVAW
QDWLDVRTTLAVEATVSSTTITHSGFAALKVKDAAVDVLRERLGARPDVDPKDPDVRIVLHLARDEATLSLDLAGAPLHR
RGYRAVTTEAPLKETLAAAVLLLGGVDPALPFADPMAGSGTLAIEHALRARRIAPGLDRAFGFQRWPSYRGGPQSAWDRM
KAAARAEILPRAPAPIVARDGHPKAIQALRLNAAAAGVEADLEISQADARDLQPSADAGAIVTNPPYGERLGGGGEGASA
AEARVAGKKLQGFYRGLAEMLVRHHGWTAVVLSGNPLLERAIPIRPAVDHRLWNGPLEAHLLKYELP
>Mature_387_residues
MRFFATCAKGTEGALRRELSGLRLPAVRGDRGGVSFEGALEAGMRACLHARTAMRVLLELARFPAPDAAALYEGARAVAW
QDWLDVRTTLAVEATVSSTTITHSGFAALKVKDAAVDVLRERLGARPDVDPKDPDVRIVLHLARDEATLSLDLAGAPLHR
RGYRAVTTEAPLKETLAAAVLLLGGVDPALPFADPMAGSGTLAIEHALRARRIAPGLDRAFGFQRWPSYRGGPQSAWDRM
KAAARAEILPRAPAPIVARDGHPKAIQALRLNAAAAGVEADLEISQADARDLQPSADAGAIVTNPPYGERLGGGGEGASA
AEARVAGKKLQGFYRGLAEMLVRHHGWTAVVLSGNPLLERAIPIRPAVDHRLWNGPLEAHLLKYELP

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=363, Percent_Identity=33.0578512396694, Blast_Score=180, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 41221; Mature: 41221

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFFATCAKGTEGALRRELSGLRLPAVRGDRGGVSFEGALEAGMRACLHARTAMRVLLEL
CCEEEECCCCCHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ARFPAPDAAALYEGARAVAWQDWLDVRTTLAVEATVSSTTITHSGFAALKVKDAAVDVLR
HHCCCCCHHHHHCCCHHHHHHHHHHHHHEEEEEEEECCEEEECCCEEEEEEHHHHHHHHH
ERLGARPDVDPKDPDVRIVLHLARDEATLSLDLAGAPLHRRGYRAVTTEAPLKETLAAAV
HHHCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHCCCEEECCCCCHHHHHHHHH
LLLGGVDPALPFADPMAGSGTLAIEHALRARRIAPGLDRAFGFQRWPSYRGGPQSAWDRM
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCHHCCCCCCCCCHHHHHHH
KAAARAEILPRAPAPIVARDGHPKAIQALRLNAAAAGVEADLEISQADARDLQPSADAGA
HHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCEECCCCHHCCCCCCCCCE
IVTNPPYGERLGGGGEGASAAEARVAGKKLQGFYRGLAEMLVRHHGWTAVVLSGNPLLER
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHH
AIPIRPAVDHRLWNGPLEAHLLKYELP
CCCCCCCHHHHHCCCCHHHEEEEECCC
>Mature Secondary Structure
MRFFATCAKGTEGALRRELSGLRLPAVRGDRGGVSFEGALEAGMRACLHARTAMRVLLEL
CCEEEECCCCCHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ARFPAPDAAALYEGARAVAWQDWLDVRTTLAVEATVSSTTITHSGFAALKVKDAAVDVLR
HHCCCCCHHHHHCCCHHHHHHHHHHHHHEEEEEEEECCEEEECCCEEEEEEHHHHHHHHH
ERLGARPDVDPKDPDVRIVLHLARDEATLSLDLAGAPLHRRGYRAVTTEAPLKETLAAAV
HHHCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHCCCEEECCCCCHHHHHHHHH
LLLGGVDPALPFADPMAGSGTLAIEHALRARRIAPGLDRAFGFQRWPSYRGGPQSAWDRM
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCHHCCCCCCCCCHHHHHHH
KAAARAEILPRAPAPIVARDGHPKAIQALRLNAAAAGVEADLEISQADARDLQPSADAGA
HHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCEECCCCHHCCCCCCCCCE
IVTNPPYGERLGGGGEGASAAEARVAGKKLQGFYRGLAEMLVRHHGWTAVVLSGNPLLER
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHH
AIPIRPAVDHRLWNGPLEAHLLKYELP
CCCCCCCHHHHHCCCCHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA