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The map label for this gene is hyfF [H]

Identifier: 220918885

GI number: 220918885

Start: 4220499

End: 4221929

Strand: Direct

Name: hyfF [H]

Synonym: A2cp1_3799

Alternate gene names: 220918885

Gene position: 4220499-4221929 (Clockwise)

Preceding gene: 220918884

Following gene: 220918886

Centisome position: 83.92

GC content: 73.65

Gene sequence:

>1431_bases
ATGGTGCTCCTCATCGCCGCCCCGCTCGCGCTCGCGCTGGTCGCGTTCGCGATGCCCTCCGCCCGGCGCCGCCCGCTGGT
GCTCCTCGCCGGTGCGCTGGTCCACACCGCCGGCGTCCTGGCGCTGCTGGCCGCCCCGCCGCCCGCCCGCGAGGGCGCCT
GGCTCGCGTTCGACCCGCTCGCGCGCGTCACGCTCCTCACCGCCACCGCGCTGTTCCTCGCCTCGGCGGTGTACGCGCAG
GGATACCTCGCCCGCCGCGCCGATCGCGACAACCGCGTGTTCGTGGGCGGCCTGCTGGTGCTGCTCTCGGCCATGACCAC
GGTGGCGCTCTCGCACCACCTCGGCCTGACCTGGGTGGCCATGGAGGCGAGCACGCTCTCCACCGCGCCCCTCATCTACT
TCAACCAGAACGCCCGCTCGCTCGAGGCCGCCTGGAAGTACCTGCTCATCGGCTCGCTCGGCATCGCGCTGGCCCTGCTC
GGCACGTTCTTCCTGGCGCTCGCCGGGGCGGGCCCGGGCGGCCCGAAGTCGCTGATGGTCGAGGACCTGATCGCGGCCGG
CGGCGCGCTCTCCCGGCCGTGGGTGCGGGCGGCGTTCGTGTTCCTGCTGGTGGGCTACGGCACGAAGATGGGCCTCGCGC
CGCTGCACTCCTGGAAGCCGGACGCCTACGGCGAGGCGCCCGGGATCCTCGGGGCGCTGCTCGCCGGCGGGCTCACCAAC
TTCGCGTTCGTCTCGGTGGTGCGCGTGTTCCAGGTGACGATCGCGGCCGGCGAGGGCGCGTTCGCGCGCGACGCGCTCAT
GGCGCTCGGCCTGCTCTCGATCGGCCTCGCCGCGGTGTTCGTGGTCGGGCAGCGCGACTTCAAGCGCATGCTCGCGTACT
CCAGCGTCGAGCACATGGGCATCCTCGCCATCGGCGTGTCGCTGGGCGGCGCCGCGACCGCCGGCGCGTTCTTCCACACC
ATGAACAACGGCCTGACCAAGGGCGTGCTGTTCCTGTCGGCCGGAAACATCCACCGCGCCTTCGGCTCGAAGACCACCGA
CGACGTGCACGGCGCCGCGCGTCGCCTGCCGCTCTCCGGGCCCCTGTTCCTGGCCGGCTTCCTCGCCATCACCGGCTCGC
CGCCGTTCGGGCCGTTCTTCAGCGAGTTCGCCATCGTGAACGGCGCGTTCGGCGCCGGACGCTACTGGGTGGGCGGGCTG
TTCCTCGCGTTCCTGGCGGTGATCTTCGTGGGCATGGCCGCGACCGTGCTCGACGTGGTGCAGGGCGACCCGTCGGACGC
GCCGCGCGTGCCCGGCACCGGGGACAGCTGGCTGACCGCCGGCCCGCCGCTGGCGCTCATGCTGGTGGTCCTGGCGCTGG
GGGTGTTCCTGCCGGCGGGGCTGAAGGACCTGTTCGGCGCCGCGGCGGCGCTCGTCGGGGGAGGCTGGTGA

Upstream 100 bases:

>100_bases
CGCCGCCGTGTTCGTCATGGGCATCGTCATGTTCCACATCAACCGCGAGTTCGCCTCGCTCGACACCGAGAAGCTCTCGG
CGCTGAAGGACTGACCGCGC

Downstream 100 bases:

>100_bases
TGGCCCGCAACACGACCGCGACCGCCGGGCACGGCGCCGCGCCCCGCGCCAGCGACGTCCTCGAGACCTGGAACGGCGAG
CCGGTGGACGCGCGCGCGGT

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MVLLIAAPLALALVAFAMPSARRRPLVLLAGALVHTAGVLALLAAPPPAREGAWLAFDPLARVTLLTATALFLASAVYAQ
GYLARRADRDNRVFVGGLLVLLSAMTTVALSHHLGLTWVAMEASTLSTAPLIYFNQNARSLEAAWKYLLIGSLGIALALL
GTFFLALAGAGPGGPKSLMVEDLIAAGGALSRPWVRAAFVFLLVGYGTKMGLAPLHSWKPDAYGEAPGILGALLAGGLTN
FAFVSVVRVFQVTIAAGEGAFARDALMALGLLSIGLAAVFVVGQRDFKRMLAYSSVEHMGILAIGVSLGGAATAGAFFHT
MNNGLTKGVLFLSAGNIHRAFGSKTTDDVHGAARRLPLSGPLFLAGFLAITGSPPFGPFFSEFAIVNGAFGAGRYWVGGL
FLAFLAVIFVGMAATVLDVVQGDPSDAPRVPGTGDSWLTAGPPLALMLVVLALGVFLPAGLKDLFGAAAALVGGGW

Sequences:

>Translated_476_residues
MVLLIAAPLALALVAFAMPSARRRPLVLLAGALVHTAGVLALLAAPPPAREGAWLAFDPLARVTLLTATALFLASAVYAQ
GYLARRADRDNRVFVGGLLVLLSAMTTVALSHHLGLTWVAMEASTLSTAPLIYFNQNARSLEAAWKYLLIGSLGIALALL
GTFFLALAGAGPGGPKSLMVEDLIAAGGALSRPWVRAAFVFLLVGYGTKMGLAPLHSWKPDAYGEAPGILGALLAGGLTN
FAFVSVVRVFQVTIAAGEGAFARDALMALGLLSIGLAAVFVVGQRDFKRMLAYSSVEHMGILAIGVSLGGAATAGAFFHT
MNNGLTKGVLFLSAGNIHRAFGSKTTDDVHGAARRLPLSGPLFLAGFLAITGSPPFGPFFSEFAIVNGAFGAGRYWVGGL
FLAFLAVIFVGMAATVLDVVQGDPSDAPRVPGTGDSWLTAGPPLALMLVVLALGVFLPAGLKDLFGAAAALVGGGW
>Mature_476_residues
MVLLIAAPLALALVAFAMPSARRRPLVLLAGALVHTAGVLALLAAPPPAREGAWLAFDPLARVTLLTATALFLASAVYAQ
GYLARRADRDNRVFVGGLLVLLSAMTTVALSHHLGLTWVAMEASTLSTAPLIYFNQNARSLEAAWKYLLIGSLGIALALL
GTFFLALAGAGPGGPKSLMVEDLIAAGGALSRPWVRAAFVFLLVGYGTKMGLAPLHSWKPDAYGEAPGILGALLAGGLTN
FAFVSVVRVFQVTIAAGEGAFARDALMALGLLSIGLAAVFVVGQRDFKRMLAYSSVEHMGILAIGVSLGGAATAGAFFHT
MNNGLTKGVLFLSAGNIHRAFGSKTTDDVHGAARRLPLSGPLFLAGFLAITGSPPFGPFFSEFAIVNGAFGAGRYWVGGL
FLAFLAVIFVGMAATVLDVVQGDPSDAPRVPGTGDSWLTAGPPLALMLVVLALGVFLPAGLKDLFGAAAALVGGGW

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Escherichia coli, GI1788831, Length=435, Percent_Identity=33.3333333333333, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1788827, Length=289, Percent_Identity=30.7958477508651, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1788829, Length=376, Percent_Identity=28.4574468085106, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI145693160, Length=255, Percent_Identity=30.1960784313725, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788613, Length=190, Percent_Identity=31.0526315789474, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI2367154, Length=328, Percent_Identity=24.390243902439, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 48918; Mature: 48918

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLLIAAPLALALVAFAMPSARRRPLVLLAGALVHTAGVLALLAAPPPAREGAWLAFDPL
CEEEEEHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHH
ARVTLLTATALFLASAVYAQGYLARRADRDNRVFVGGLLVLLSAMTTVALSHHLGLTWVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEE
MEASTLSTAPLIYFNQNARSLEAAWKYLLIGSLGIALALLGTFFLALAGAGPGGPKSLMV
EECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
EDLIAAGGALSRPWVRAAFVFLLVGYGTKMGLAPLHSWKPDAYGEAPGILGALLAGGLTN
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHH
FAFVSVVRVFQVTIAAGEGAFARDALMALGLLSIGLAAVFVVGQRDFKRMLAYSSVEHMG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCE
ILAIGVSLGGAATAGAFFHTMNNGLTKGVLFLSAGNIHRAFGSKTTDDVHGAARRLPLSG
EEEEECCCCCCHHHHHHHHHHHCCCHHCEEEEECCCHHHHHCCCCCHHHHHHHHCCCCCC
PLFLAGFLAITGSPPFGPFFSEFAIVNGAFGAGRYWVGGLFLAFLAVIFVGMAATVLDVV
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGDPSDAPRVPGTGDSWLTAGPPLALMLVVLALGVFLPAGLKDLFGAAAALVGGGW
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVLLIAAPLALALVAFAMPSARRRPLVLLAGALVHTAGVLALLAAPPPAREGAWLAFDPL
CEEEEEHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHH
ARVTLLTATALFLASAVYAQGYLARRADRDNRVFVGGLLVLLSAMTTVALSHHLGLTWVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEE
MEASTLSTAPLIYFNQNARSLEAAWKYLLIGSLGIALALLGTFFLALAGAGPGGPKSLMV
EECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
EDLIAAGGALSRPWVRAAFVFLLVGYGTKMGLAPLHSWKPDAYGEAPGILGALLAGGLTN
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHH
FAFVSVVRVFQVTIAAGEGAFARDALMALGLLSIGLAAVFVVGQRDFKRMLAYSSVEHMG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCE
ILAIGVSLGGAATAGAFFHTMNNGLTKGVLFLSAGNIHRAFGSKTTDDVHGAARRLPLSG
EEEEECCCCCCHHHHHHHHHHHCCCHHCEEEEECCCHHHHHCCCCCHHHHHHHHCCCCCC
PLFLAGFLAITGSPPFGPFFSEFAIVNGAFGAGRYWVGGLFLAFLAVIFVGMAATVLDVV
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGDPSDAPRVPGTGDSWLTAGPPLALMLVVLALGVFLPAGLKDLFGAAAALVGGGW
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]