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The map label for this gene is hycE [H]

Identifier: 220918886

GI number: 220918886

Start: 4221929

End: 4223494

Strand: Direct

Name: hycE [H]

Synonym: A2cp1_3800

Alternate gene names: 220918886

Gene position: 4221929-4223494 (Clockwise)

Preceding gene: 220918885

Following gene: 220918887

Centisome position: 83.95

GC content: 76.31

Gene sequence:

>1566_bases
ATGGCCCGCAACACGACCGCGACCGCCGGGCACGGCGCCGCGCCCCGCGCCAGCGACGTCCTCGAGACCTGGAACGGCGA
GCCGGTGGACGCGCGCGCGGTGCCGGTGCACGAGCCCGCCCGGTTCAACGAGATCGTCCTCGCCGCGCACCGCGGCGGCG
GGCGCCTCGCCGCGCTGTTCGGCCGCCCCGACGCCGGGGGCGGCGTCCTCGTGACCGCGGTGCTGGCGGACGACCCGGAG
GGCCGGCTGGCGCTGCTCGCCACCCGCGTCGCCGACGCGTTCCCTTCGATCGCGGCCGAGCTGCCCGCGGCCCAGGCGTT
CGAGCGCGAGCTGGCGGAGCAGTGGGGGCTGCGCCCCGAGGGCCACCCCTGGCTGAAGCCGCTCCGCTTCGAGCCGCCGC
GCCGCGCCGCGCCGGATCCGTTCGGCCGCCAGGACCCGCGCGCCACCATCCCCGGCGAGTACCCGTTCTTCCGGGTCGAG
GGCGAGGAGGTGCACGAGGTCGCGGTGGGCCCGGTGCACGCGGGCATCATCGAGCCGGGCCACTTCCGCTTCCAGTGCCA
CGGCGAGCGCGTGTTCCACCTCGAGATCGTGCTCGGCTACCAGCACCGCGGCGTGGAGCGCCTCCTGCGCGGCGGCCCGG
ACCGCCGCTCCATGGCGCTCGCCGAGTCGATCGCCGGCGACACCGCGGTGGGCCACGGGCTCGCCTACGCGAAGGCGGTC
GAGTCGCTCGCCGGCGTGGCGCCGACCGCGCGCGCCATGTCGCTGCGCGGGATCGGGCTCGAGCTGGAGCGGATCGCGAA
CCACGTCGGCGACCTGGGCGCGCTGGCCGGCGACGTGGGCTTCCTGCCCACGGCGTCGTACTGCGGCGCGCTCCGCGCCG
AGTTCCTGAACGCGGTGGCGGAGATCTGCGGCAACCGCTTCGGGCGCAGCCTGGTGTGCCCGGGCGGCGTGAAGCACGAC
CTCGACGCGGAGGCGGCGCGCGCGCTGGCCGCCCGCGTCCGCGCGGCCTGGGAGAAGACCCGGCGCGCGGCGTCGCTCAT
GTTCGAGTCGCCCTCGGTGCGCGCCCGGTTCGAGGGCACCGGCACCGTGCCCACCGCGCTGGCGCAGGCGCTCGGGATGG
TCGGGCCGGCCGCGCGCGCCAGCGGCGTGGACCGCGACGTCCGGCGCGACCACGCGTTCGGCATCTACCGCTTCTCCCAC
GTGGCGGTGACCACCGCCGAGAACGGTGACGTGCTGGCCCGCGCGCTCATCCGCCTGCTCGAGGCCGAGCGGAGCGTGGA
GTTCGTGCTCGACCAGCTCGAGCGGCTGCCCGGCGGCCCCTCGCGCACGGCGCCCGGGCCGCTCGCGCCCGGCGCGCTGG
TGGTCTCCATGGTGGAGGGCTGGCGCGGGGAGATCGCCCACGTCGCGGTGACCGACGCGCAGGGGCGCCTCGAGGCCTAC
AAGGTGAAGGACCCGTCCTTCCACAACTGGTTCGGGCTCGCCATGGCGCTCCGCGACGGCCAGATCTCCGACTTCCCGCT
CTGCAACAAGAGCTTCAACCTCTCCTACGCGGGGCACGACCTGTGA

Upstream 100 bases:

>100_bases
TGGCGCTCATGCTGGTGGTCCTGGCGCTGGGGGTGTTCCTGCCGGCGGGGCTGAAGGACCTGTTCGGCGCCGCGGCGGCG
CTCGTCGGGGGAGGCTGGTG

Downstream 100 bases:

>100_bases
TCGACATCCTGAAGTTCCGGCTCGGCTACGGGAACCAGACCGTGGCCTTCCCCGACGGCCCGCCCCGCTTCCCGGCGCGC
TTCCGGGGGCGGCCGGTGGT

Product: NADH-ubiquinone oxidoreductase chain 49kDa

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MARNTTATAGHGAAPRASDVLETWNGEPVDARAVPVHEPARFNEIVLAAHRGGGRLAALFGRPDAGGGVLVTAVLADDPE
GRLALLATRVADAFPSIAAELPAAQAFERELAEQWGLRPEGHPWLKPLRFEPPRRAAPDPFGRQDPRATIPGEYPFFRVE
GEEVHEVAVGPVHAGIIEPGHFRFQCHGERVFHLEIVLGYQHRGVERLLRGGPDRRSMALAESIAGDTAVGHGLAYAKAV
ESLAGVAPTARAMSLRGIGLELERIANHVGDLGALAGDVGFLPTASYCGALRAEFLNAVAEICGNRFGRSLVCPGGVKHD
LDAEAARALAARVRAAWEKTRRAASLMFESPSVRARFEGTGTVPTALAQALGMVGPAARASGVDRDVRRDHAFGIYRFSH
VAVTTAENGDVLARALIRLLEAERSVEFVLDQLERLPGGPSRTAPGPLAPGALVVSMVEGWRGEIAHVAVTDAQGRLEAY
KVKDPSFHNWFGLAMALRDGQISDFPLCNKSFNLSYAGHDL

Sequences:

>Translated_521_residues
MARNTTATAGHGAAPRASDVLETWNGEPVDARAVPVHEPARFNEIVLAAHRGGGRLAALFGRPDAGGGVLVTAVLADDPE
GRLALLATRVADAFPSIAAELPAAQAFERELAEQWGLRPEGHPWLKPLRFEPPRRAAPDPFGRQDPRATIPGEYPFFRVE
GEEVHEVAVGPVHAGIIEPGHFRFQCHGERVFHLEIVLGYQHRGVERLLRGGPDRRSMALAESIAGDTAVGHGLAYAKAV
ESLAGVAPTARAMSLRGIGLELERIANHVGDLGALAGDVGFLPTASYCGALRAEFLNAVAEICGNRFGRSLVCPGGVKHD
LDAEAARALAARVRAAWEKTRRAASLMFESPSVRARFEGTGTVPTALAQALGMVGPAARASGVDRDVRRDHAFGIYRFSH
VAVTTAENGDVLARALIRLLEAERSVEFVLDQLERLPGGPSRTAPGPLAPGALVVSMVEGWRGEIAHVAVTDAQGRLEAY
KVKDPSFHNWFGLAMALRDGQISDFPLCNKSFNLSYAGHDL
>Mature_520_residues
ARNTTATAGHGAAPRASDVLETWNGEPVDARAVPVHEPARFNEIVLAAHRGGGRLAALFGRPDAGGGVLVTAVLADDPEG
RLALLATRVADAFPSIAAELPAAQAFERELAEQWGLRPEGHPWLKPLRFEPPRRAAPDPFGRQDPRATIPGEYPFFRVEG
EEVHEVAVGPVHAGIIEPGHFRFQCHGERVFHLEIVLGYQHRGVERLLRGGPDRRSMALAESIAGDTAVGHGLAYAKAVE
SLAGVAPTARAMSLRGIGLELERIANHVGDLGALAGDVGFLPTASYCGALRAEFLNAVAEICGNRFGRSLVCPGGVKHDL
DAEAARALAARVRAAWEKTRRAASLMFESPSVRARFEGTGTVPTALAQALGMVGPAARASGVDRDVRRDHAFGIYRFSHV
AVTTAENGDVLARALIRLLEAERSVEFVLDQLERLPGGPSRTAPGPLAPGALVVSMVEGWRGEIAHVAVTDAQGRLEAYK
VKDPSFHNWFGLAMALRDGQISDFPLCNKSFNLSYAGHDL

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=348, Percent_Identity=21.8390804597701, Blast_Score=83, Evalue=8e-16,
Organism=Homo sapiens, GI260898743, Length=348, Percent_Identity=21.8390804597701, Blast_Score=82, Evalue=9e-16,
Organism=Escherichia coli, GI1789076, Length=495, Percent_Identity=29.4949494949495, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1788832, Length=489, Percent_Identity=27.4028629856851, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI145693162, Length=479, Percent_Identity=23.7995824634656, Blast_Score=105, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17568379, Length=253, Percent_Identity=25.296442687747, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17555284, Length=253, Percent_Identity=24.1106719367589, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221459469, Length=301, Percent_Identity=23.9202657807309, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24638644, Length=331, Percent_Identity=22.6586102719033, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 55599; Mature: 55467

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARNTTATAGHGAAPRASDVLETWNGEPVDARAVPVHEPARFNEIVLAAHRGGGRLAALF
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
GRPDAGGGVLVTAVLADDPEGRLALLATRVADAFPSIAAELPAAQAFERELAEQWGLRPE
CCCCCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
GHPWLKPLRFEPPRRAAPDPFGRQDPRATIPGEYPFFRVEGEEVHEVAVGPVHAGIIEPG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHEECCCEEECCCCCC
HFRFQCHGERVFHLEIVLGYQHRGVERLLRGGPDRRSMALAESIAGDTAVGHGLAYAKAV
CEEEEECCCEEEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
ESLAGVAPTARAMSLRGIGLELERIANHVGDLGALAGDVGFLPTASYCGALRAEFLNAVA
HHHHCCCCCHHHHEECCCCEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
EICGNRFGRSLVCPGGVKHDLDAEAARALAARVRAAWEKTRRAASLMFESPSVRARFEGT
HHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
GTVPTALAQALGMVGPAARASGVDRDVRRDHAFGIYRFSHVAVTTAENGDVLARALIRLL
CCHHHHHHHHHHCCCCHHHHCCCCHHHHHHCCEEEEEEEEEEEEECCCCHHHHHHHHHHH
EAERSVEFVLDQLERLPGGPSRTAPGPLAPGALVVSMVEGWRGEIAHVAVTDAQGRLEAY
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEEE
KVKDPSFHNWFGLAMALRDGQISDFPLCNKSFNLSYAGHDL
EECCCCCCHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ARNTTATAGHGAAPRASDVLETWNGEPVDARAVPVHEPARFNEIVLAAHRGGGRLAALF
CCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
GRPDAGGGVLVTAVLADDPEGRLALLATRVADAFPSIAAELPAAQAFERELAEQWGLRPE
CCCCCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
GHPWLKPLRFEPPRRAAPDPFGRQDPRATIPGEYPFFRVEGEEVHEVAVGPVHAGIIEPG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHEECCCEEECCCCCC
HFRFQCHGERVFHLEIVLGYQHRGVERLLRGGPDRRSMALAESIAGDTAVGHGLAYAKAV
CEEEEECCCEEEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
ESLAGVAPTARAMSLRGIGLELERIANHVGDLGALAGDVGFLPTASYCGALRAEFLNAVA
HHHHCCCCCHHHHEECCCCEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
EICGNRFGRSLVCPGGVKHDLDAEAARALAARVRAAWEKTRRAASLMFESPSVRARFEGT
HHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
GTVPTALAQALGMVGPAARASGVDRDVRRDHAFGIYRFSHVAVTTAENGDVLARALIRLL
CCHHHHHHHHHHCCCCHHHHCCCCHHHHHHCCEEEEEEEEEEEEECCCCHHHHHHHHHHH
EAERSVEFVLDQLERLPGGPSRTAPGPLAPGALVVSMVEGWRGEIAHVAVTDAQGRLEAY
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEEE
KVKDPSFHNWFGLAMALRDGQISDFPLCNKSFNLSYAGHDL
EECCCCCCHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]