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The map label for this gene is narK [H]

Identifier: 220916879

GI number: 220916879

Start: 1985045

End: 1986472

Strand: Reverse

Name: narK [H]

Synonym: A2cp1_1775

Alternate gene names: 220916879

Gene position: 1986472-1985045 (Counterclockwise)

Preceding gene: 220916880

Following gene: 220916874

Centisome position: 39.5

GC content: 73.6

Gene sequence:

>1428_bases
ATGAGCCCCGTCCCCGCCCCGGCGCCCGTTCCCGAGCCCGTCACGCCCCCGCGCCGCCGCGCGCGCCTCATCACGCAGTG
GGACCCGGAGGACCGCGCCTTCTGGAGCGCCGCGGGCCGCCGGGTGGCCCGCCGCAACCTCTGGCTGTCGATCTTCGCCC
TGTTCCTCGCGTTCGCGGTCTGGATGGTGTGGAGCGTGGTGGCGGTGAAGCTGCCCGCCATCGGCTTCCGCTTCACCACC
AACCAGCTCTTCTGGCTGGCCGCCCTCCCCGGCCTCTCCGGCGCGACGCTGCGCATCTTCTACGCGTTCGCGGTCCCGGT
CTTCGGCGGCCGCCGCTTCACCGCGCTCTCCACCGCCTCGCTGCTGCTGCCGGCGATCGGCATCGGCGTCGCGGTGCAGG
ACCCGGACACCTCCTACTCGACGTTCCTGGTGCTCGCGCTCCTCACCGGCCTCGGCGGCGCGAACTTCGCCTCGAGCATG
TCCAACATCAGCTTCTTCTTCCCGCGCGCGGAGAAGGGCACCGCGCTCGGCCTGAACGCCGGCCTCGGCAACCTGGGCGT
CTCCGGCGTGCAGCTCGCGGTCCCGCTGGTGGTGGCGGCCGCGCTGCTCGGCCCGCTCGCGGGCGGGCCGCAGGCGCTGC
CCGGCGGCAAGCAGGTCTGGCTGCAGAACGCCGCGTTCGTGTGGGTGCCGCTCATCGTGGTCGCATCCGTGCTCGCCTGG
TTCGGCATGGACGACCTCGCCAGCGCGAAGGCCTCCTTCCGCGAGCAGTCGGTGATCTTCCGGCGCAAGCACAACTGGCT
CGTGTCGCTGCTGTACCTCGGCACGTTCGGCTCGTTCATCGGCTACTCCGCCGGCCTCGCGCTGCTCACCCAGGCGTCCT
TCCCGGCGGTGGACCCGACCCGGTACGCGTTCGTGGGGCCGCTGGTGGGCGGCCTGGCGCGGCCGGTGGGCGGGTGGCTC
GCGGACCGGCTGGGCGGCGCCCGGGTGACGCTGGTCACGTTCCTGGTGATGGGGCTCGCGGCGGTGGGCGTGCTCCTGGC
GCTGCCCCGCGGCGGCGGGCCCGGCAGCTTCGGCGGCTTCCTCGCGGCGTTCGTGGTGCTCTTCACCGCGACCGGGATCG
GGAACGGCTCGGTGTACCGGATGATCCCGGTGATCTTCTCCACCTTCCACCAGCGGCGCGCCGGCACCGATCCGGTCGCG
CTGGCCCAGGCGCAGAAGGACGCGCTCCGCGAGGGCGGCGCGGTGGTCGGCTTCGTCTCCGCCATCGCCGCCTACGGCGC
GTTCTTCGTGCCGCGCACCTTCGGCATGTCGGTGGAGCTGACCGGCGCGCCGCACGGCGCGGTGCTGGCGTTCGTGGCCT
ACTACGCGGGCTGCATCGCGCTGGTGTGGTGGTACTACGCCCGGCCGCGGGCCGAGATCCCGGCGTGA

Upstream 100 bases:

>100_bases
CCACGCCACCAGCCTGCCGCTCACATACCTCTGGCGCGCGCCGCAGCGGGTGGTGTGGCGCCGAGCGCCCTCCGCGGCGT
CCCATGACGCGAGGTGACCG

Downstream 100 bases:

>100_bases
CGGTGCCGTGCCGGGGCGACGCGCCGCCGCGCGCCCCGGCTCACCGCCGGCCGCCCTGCGCCCCCATCCGCGCGAACGCC
GCCGCGAGCCCGTCCACGCG

Product: nitrite transporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSPVPAPAPVPEPVTPPRRRARLITQWDPEDRAFWSAAGRRVARRNLWLSIFALFLAFAVWMVWSVVAVKLPAIGFRFTT
NQLFWLAALPGLSGATLRIFYAFAVPVFGGRRFTALSTASLLLPAIGIGVAVQDPDTSYSTFLVLALLTGLGGANFASSM
SNISFFFPRAEKGTALGLNAGLGNLGVSGVQLAVPLVVAAALLGPLAGGPQALPGGKQVWLQNAAFVWVPLIVVASVLAW
FGMDDLASAKASFREQSVIFRRKHNWLVSLLYLGTFGSFIGYSAGLALLTQASFPAVDPTRYAFVGPLVGGLARPVGGWL
ADRLGGARVTLVTFLVMGLAAVGVLLALPRGGGPGSFGGFLAAFVVLFTATGIGNGSVYRMIPVIFSTFHQRRAGTDPVA
LAQAQKDALREGGAVVGFVSAIAAYGAFFVPRTFGMSVELTGAPHGAVLAFVAYYAGCIALVWWYYARPRAEIPA

Sequences:

>Translated_475_residues
MSPVPAPAPVPEPVTPPRRRARLITQWDPEDRAFWSAAGRRVARRNLWLSIFALFLAFAVWMVWSVVAVKLPAIGFRFTT
NQLFWLAALPGLSGATLRIFYAFAVPVFGGRRFTALSTASLLLPAIGIGVAVQDPDTSYSTFLVLALLTGLGGANFASSM
SNISFFFPRAEKGTALGLNAGLGNLGVSGVQLAVPLVVAAALLGPLAGGPQALPGGKQVWLQNAAFVWVPLIVVASVLAW
FGMDDLASAKASFREQSVIFRRKHNWLVSLLYLGTFGSFIGYSAGLALLTQASFPAVDPTRYAFVGPLVGGLARPVGGWL
ADRLGGARVTLVTFLVMGLAAVGVLLALPRGGGPGSFGGFLAAFVVLFTATGIGNGSVYRMIPVIFSTFHQRRAGTDPVA
LAQAQKDALREGGAVVGFVSAIAAYGAFFVPRTFGMSVELTGAPHGAVLAFVAYYAGCIALVWWYYARPRAEIPA
>Mature_474_residues
SPVPAPAPVPEPVTPPRRRARLITQWDPEDRAFWSAAGRRVARRNLWLSIFALFLAFAVWMVWSVVAVKLPAIGFRFTTN
QLFWLAALPGLSGATLRIFYAFAVPVFGGRRFTALSTASLLLPAIGIGVAVQDPDTSYSTFLVLALLTGLGGANFASSMS
NISFFFPRAEKGTALGLNAGLGNLGVSGVQLAVPLVVAAALLGPLAGGPQALPGGKQVWLQNAAFVWVPLIVVASVLAWF
GMDDLASAKASFREQSVIFRRKHNWLVSLLYLGTFGSFIGYSAGLALLTQASFPAVDPTRYAFVGPLVGGLARPVGGWLA
DRLGGARVTLVTFLVMGLAAVGVLLALPRGGGPGSFGGFLAAFVVLFTATGIGNGSVYRMIPVIFSTFHQRRAGTDPVAL
AQAQKDALREGGAVVGFVSAIAAYGAFFVPRTFGMSVELTGAPHGAVLAFVAYYAGCIALVWWYYARPRAEIPA

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=457, Percent_Identity=49.890590809628, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1787743, Length=457, Percent_Identity=50.328227571116, Blast_Score=402, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50067; Mature: 49936

Theoretical pI: Translated: 11.22; Mature: 11.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPVPAPAPVPEPVTPPRRRARLITQWDPEDRAFWSAAGRRVARRNLWLSIFALFLAFAV
CCCCCCCCCCCCCCCCHHHHCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WMVWSVVAVKLPAIGFRFTTNQLFWLAALPGLSGATLRIFYAFAVPVFGGRRFTALSTAS
HHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHH
LLLPAIGIGVAVQDPDTSYSTFLVLALLTGLGGANFASSMSNISFFFPRAEKGTALGLNA
HHHHHHHCCEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCCCEEEECC
GLGNLGVSGVQLAVPLVVAAALLGPLAGGPQALPGGKQVWLQNAAFVWVPLIVVASVLAW
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHH
FGMDDLASAKASFREQSVIFRRKHNWLVSLLYLGTFGSFIGYSAGLALLTQASFPAVDPT
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RYAFVGPLVGGLARPVGGWLADRLGGARVTLVTFLVMGLAAVGVLLALPRGGGPGSFGGF
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LAAFVVLFTATGIGNGSVYRMIPVIFSTFHQRRAGTDPVALAQAQKDALREGGAVVGFVS
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHH
AIAAYGAFFVPRTFGMSVELTGAPHGAVLAFVAYYAGCIALVWWYYARPRAEIPA
HHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SPVPAPAPVPEPVTPPRRRARLITQWDPEDRAFWSAAGRRVARRNLWLSIFALFLAFAV
CCCCCCCCCCCCCCCHHHHCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WMVWSVVAVKLPAIGFRFTTNQLFWLAALPGLSGATLRIFYAFAVPVFGGRRFTALSTAS
HHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHH
LLLPAIGIGVAVQDPDTSYSTFLVLALLTGLGGANFASSMSNISFFFPRAEKGTALGLNA
HHHHHHHCCEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCCCEEEECC
GLGNLGVSGVQLAVPLVVAAALLGPLAGGPQALPGGKQVWLQNAAFVWVPLIVVASVLAW
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHH
FGMDDLASAKASFREQSVIFRRKHNWLVSLLYLGTFGSFIGYSAGLALLTQASFPAVDPT
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RYAFVGPLVGGLARPVGGWLADRLGGARVTLVTFLVMGLAAVGVLLALPRGGGPGSFGGF
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LAAFVVLFTATGIGNGSVYRMIPVIFSTFHQRRAGTDPVALAQAQKDALREGGAVVGFVS
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHH
AIAAYGAFFVPRTFGMSVELTGAPHGAVLAFVAYYAGCIALVWWYYARPRAEIPA
HHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]