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The map label for this gene is braC [H]

Identifier: 220916874

GI number: 220916874

Start: 1979048

End: 1980280

Strand: Reverse

Name: braC [H]

Synonym: A2cp1_1770

Alternate gene names: 220916874

Gene position: 1980280-1979048 (Counterclockwise)

Preceding gene: 220916879

Following gene: 220916873

Centisome position: 39.37

GC content: 68.86

Gene sequence:

>1233_bases
ATGACCAAGCTGTTGGGTACCCTGCTTCTCTGTGCACTTGCCCTGCCCGCCGGGGCAGCCGACTCCGTCAAGATCGCCGT
CACCGGGCCGTTCAGCGGCGGGTCCGCGCCCATGGGCGCCTCGATGCGGGACGGCGCGAAGCTCGCCGTCGCCGAGATCA
ACGCCGCCGGCGGCGTCCAGGTCGGCGCGCGCAAGCTGAAGATCGAGGTGGTCGAGCGCGACGACGAGGCCAAGAACGAG
CGCGGCGCGCTCATCGGCCAGGAGCTGGCCTCGATGGCCGACCTCGCCGGCGTGATCGGCTCGGTCAACACCGGCGTGGT
GATGGCCGGCGACAAGCACCTCCAGGAGAAGGGCATCACCAAGATCATCACGCCCGCCGCCGGCTCGGCCTCGATGACCC
AGTGGGCGAAGGGCGCCGAGGGGCCGAAGACGCTCTCCATCTTCCGCTTCGCCGCGCACGACGGCATCCAGGCCGCCATG
GTGGTCGAGGAGGCGATCAACCGGAAGTTCACCAAGGTCGCGCTCCTGCACGACTCCACCAACTACGGCGTGTCCGGCCG
CGACGACCTGCTCCGGCAGATCAAGCTGCAGGGCGACAAGCTCCAGGTGATCGCCACCGAGAAGTTCAACATCGGCGACA
AGGACATGACCGCGCAGCTGCTCCGCGCCAAGGCCGGCGGCGCGCAGGCGATCCTGATCTGGGGCATCGGGCCCGAGCTC
GCCGTGGCCGCGAACGGCATGGCCAAGCTGAACATCAAGGCGCCGCTCATCGGCGGCTGGACGCTGTCGATGTCCAACTA
CATCGACAACGCCGGGAAGAACGGCAACGGCACGCTCATGCCGCAGACGTTCATCGAGGAGCCGGTCACGCCGAAGGCGA
AGGCGTTCATCGAGGCGTACCACAAGGCGTACGGCGTGACCCGGATCCCGTCGCCCGTCTCCGCCGCGCAGGGCTACGAC
GCCGTCCACCTGTTCGCCGCCGCGGTGAAGCAGGCCGGCACCACCGACAGCCGCAAGATCGCCGAGGCGCTCGAGGACCT
GAAGGAGCCGGTGCCGGGCGTGATCGCGACCTGGATCCACCCGTACACGAAGTGGGATCCGTCCAACGTCGAGACGCACG
AGGCGTTCCGCCGCGGGCAGGTGGTGATGGGCATGGTCAAGGACGGGCGCGTGGTGTTCGCGAACGAGTCCGACCGCGCC
CGCCTCGCGCAGGGCGCCCAGAAGAAGATGTAG

Upstream 100 bases:

>100_bases
CCGATTCGTCGTGCTAGCGTAGCGCGCTCGCGCTTATTTTGCGTGTCCCGCAAGGTATTTTTTGCGTTCACTCCGCGTCA
CCAGCCACGGAGCACCATCC

Downstream 100 bases:

>100_bases
CCGCGCGGGTGCGCGGGGAGGAGTCCCCCTCCTCCCCGCGCCCGCAGGCCTCTCCCCCGCCTCCCCGAGGACCAGACCCG
CGTGCCAGCGTCGATCATCG

Product: Extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MTKLLGTLLLCALALPAGAADSVKIAVTGPFSGGSAPMGASMRDGAKLAVAEINAAGGVQVGARKLKIEVVERDDEAKNE
RGALIGQELASMADLAGVIGSVNTGVVMAGDKHLQEKGITKIITPAAGSASMTQWAKGAEGPKTLSIFRFAAHDGIQAAM
VVEEAINRKFTKVALLHDSTNYGVSGRDDLLRQIKLQGDKLQVIATEKFNIGDKDMTAQLLRAKAGGAQAILIWGIGPEL
AVAANGMAKLNIKAPLIGGWTLSMSNYIDNAGKNGNGTLMPQTFIEEPVTPKAKAFIEAYHKAYGVTRIPSPVSAAQGYD
AVHLFAAAVKQAGTTDSRKIAEALEDLKEPVPGVIATWIHPYTKWDPSNVETHEAFRRGQVVMGMVKDGRVVFANESDRA
RLAQGAQKKM

Sequences:

>Translated_410_residues
MTKLLGTLLLCALALPAGAADSVKIAVTGPFSGGSAPMGASMRDGAKLAVAEINAAGGVQVGARKLKIEVVERDDEAKNE
RGALIGQELASMADLAGVIGSVNTGVVMAGDKHLQEKGITKIITPAAGSASMTQWAKGAEGPKTLSIFRFAAHDGIQAAM
VVEEAINRKFTKVALLHDSTNYGVSGRDDLLRQIKLQGDKLQVIATEKFNIGDKDMTAQLLRAKAGGAQAILIWGIGPEL
AVAANGMAKLNIKAPLIGGWTLSMSNYIDNAGKNGNGTLMPQTFIEEPVTPKAKAFIEAYHKAYGVTRIPSPVSAAQGYD
AVHLFAAAVKQAGTTDSRKIAEALEDLKEPVPGVIATWIHPYTKWDPSNVETHEAFRRGQVVMGMVKDGRVVFANESDRA
RLAQGAQKKM
>Mature_409_residues
TKLLGTLLLCALALPAGAADSVKIAVTGPFSGGSAPMGASMRDGAKLAVAEINAAGGVQVGARKLKIEVVERDDEAKNER
GALIGQELASMADLAGVIGSVNTGVVMAGDKHLQEKGITKIITPAAGSASMTQWAKGAEGPKTLSIFRFAAHDGIQAAMV
VEEAINRKFTKVALLHDSTNYGVSGRDDLLRQIKLQGDKLQVIATEKFNIGDKDMTAQLLRAKAGGAQAILIWGIGPELA
VAANGMAKLNIKAPLIGGWTLSMSNYIDNAGKNGNGTLMPQTFIEEPVTPKAKAFIEAYHKAYGVTRIPSPVSAAQGYDA
VHLFAAAVKQAGTTDSRKIAEALEDLKEPVPGVIATWIHPYTKWDPSNVETHEAFRRGQVVMGMVKDGRVVFANESDRAR
LAQGAQKKM

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=344, Percent_Identity=24.7093023255814, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 43176; Mature: 43045

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLLGTLLLCALALPAGAADSVKIAVTGPFSGGSAPMGASMRDGAKLAVAEINAAGGVQ
CHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCE
VGARKLKIEVVERDDEAKNERGALIGQELASMADLAGVIGSVNTGVVMAGDKHLQEKGIT
ECCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHCCCE
KIITPAAGSASMTQWAKGAEGPKTLSIFRFAAHDGIQAAMVVEEAINRKFTKVALLHDST
EEECCCCCCCHHHHHHCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCEEEEEEEECCC
NYGVSGRDDLLRQIKLQGDKLQVIATEKFNIGDKDMTAQLLRAKAGGAQAILIWGIGPEL
CCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCC
AVAANGMAKLNIKAPLIGGWTLSMSNYIDNAGKNGNGTLMPQTFIEEPVTPKAKAFIEAY
EEECCCEEEEEEECCEECCEEEEHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHH
HKAYGVTRIPSPVSAAQGYDAVHLFAAAVKQAGTTDSRKIAEALEDLKEPVPGVIATWIH
HHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCC
PYTKWDPSNVETHEAFRRGQVVMGMVKDGRVVFANESDRARLAQGAQKKM
CCCCCCCCCCHHHHHHHCCCEEEEEECCCEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TKLLGTLLLCALALPAGAADSVKIAVTGPFSGGSAPMGASMRDGAKLAVAEINAAGGVQ
HHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCE
VGARKLKIEVVERDDEAKNERGALIGQELASMADLAGVIGSVNTGVVMAGDKHLQEKGIT
ECCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHCCCE
KIITPAAGSASMTQWAKGAEGPKTLSIFRFAAHDGIQAAMVVEEAINRKFTKVALLHDST
EEECCCCCCCHHHHHHCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCEEEEEEEECCC
NYGVSGRDDLLRQIKLQGDKLQVIATEKFNIGDKDMTAQLLRAKAGGAQAILIWGIGPEL
CCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCC
AVAANGMAKLNIKAPLIGGWTLSMSNYIDNAGKNGNGTLMPQTFIEEPVTPKAKAFIEAY
EEECCCEEEEEEECCEECCEEEEHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHH
HKAYGVTRIPSPVSAAQGYDAVHLFAAAVKQAGTTDSRKIAEALEDLKEPVPGVIATWIH
HHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCC
PYTKWDPSNVETHEAFRRGQVVMGMVKDGRVVFANESDRARLAQGAQKKM
CCCCCCCCCCHHHHHHHCCCEEEEEECCCEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]