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The map label for this gene is narI [H]

Identifier: 220916880

GI number: 220916880

Start: 1986476

End: 1987159

Strand: Reverse

Name: narI [H]

Synonym: A2cp1_1776

Alternate gene names: 220916880

Gene position: 1987159-1986476 (Counterclockwise)

Preceding gene: 220916881

Following gene: 220916879

Centisome position: 39.51

GC content: 74.56

Gene sequence:

>684_bases
ATGAGCGACACGTTCCTGTTCGCGGTCTTCCCCTACCTCGCGACCGTCTTCGCGGTCGGCGGCGCGATCTACCGGCGGCG
GGCGCTGGGCGGCTCGGTGAGCGCCCGCTCGTCGCAGCTCATCGAGGGGCGCGCGCTGCGCTGGGGCTCGATCGCGTGGC
ACCACGGCATCCTCGCCATCCTGGCCGCCCACCTGCTCGCGGCGGTCGCGCCGGGCGCCTGGGGCCGGCTGCTCGGCGCG
CCGGCCCGGCTGTACGCGCTCGAGATCACCGGGATGGCGCTGGCGATGCTGGCGGTGTTCGGGATCGCGCTCCTGCTCGT
GCGGCGCACCGCGCTGGCCCGGCTGACGACGCCGATGGACTGGGTGGTGCTCTCGCTGCTGCTCCTGCAGGCCGCGACCG
GGCTGTGGATCGCCTACGCGCTCCGCTGGGGCGGCCTCTGGTACCTGCACACCGCGGCGCCGTGGCTCGCCTCGCTCGCG
ACGCTCTCGCCGCGGGTGGACGCGATGGCGGTGCTGCCACCGTTGGTGAAGGTGCACGTGGTGAACGCGTTCCTGCTGCT
CGCGCTCGTGCCGGTGTCGCGCCTGGTCCACGCCACCAGCCTGCCGCTCACATACCTCTGGCGCGCGCCGCAGCGGGTGG
TGTGGCGCCGAGCGCCCTCCGCGGCGTCCCATGACGCGAGGTGA

Upstream 100 bases:

>100_bases
TCGCGGATCGCGCGAACCCCTACCGCGAGCTGCTGGTGGCGGTGCGCGCGGTGTTCGCCGCGCCGGCCGCCGCCCGGCCT
CGCGGCGCGGAGGCGAGGCC

Downstream 100 bases:

>100_bases
CCGATGAGCCCCGTCCCCGCCCCGGCGCCCGTTCCCGAGCCCGTCACGCCCCCGCGCCGCCGCGCGCGCCTCATCACGCA
GTGGGACCCGGAGGACCGCG

Product: Nitrate reductase gamma subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MSDTFLFAVFPYLATVFAVGGAIYRRRALGGSVSARSSQLIEGRALRWGSIAWHHGILAILAAHLLAAVAPGAWGRLLGA
PARLYALEITGMALAMLAVFGIALLLVRRTALARLTTPMDWVVLSLLLLQAATGLWIAYALRWGGLWYLHTAAPWLASLA
TLSPRVDAMAVLPPLVKVHVVNAFLLLALVPVSRLVHATSLPLTYLWRAPQRVVWRRAPSAASHDAR

Sequences:

>Translated_227_residues
MSDTFLFAVFPYLATVFAVGGAIYRRRALGGSVSARSSQLIEGRALRWGSIAWHHGILAILAAHLLAAVAPGAWGRLLGA
PARLYALEITGMALAMLAVFGIALLLVRRTALARLTTPMDWVVLSLLLLQAATGLWIAYALRWGGLWYLHTAAPWLASLA
TLSPRVDAMAVLPPLVKVHVVNAFLLLALVPVSRLVHATSLPLTYLWRAPQRVVWRRAPSAASHDAR
>Mature_226_residues
SDTFLFAVFPYLATVFAVGGAIYRRRALGGSVSARSSQLIEGRALRWGSIAWHHGILAILAAHLLAAVAPGAWGRLLGAP
ARLYALEITGMALAMLAVFGIALLLVRRTALARLTTPMDWVVLSLLLLQAATGLWIAYALRWGGLWYLHTAAPWLASLAT
LSPRVDAMAVLPPLVKVHVVNAFLLLALVPVSRLVHATSLPLTYLWRAPQRVVWRRAPSAASHDAR

Specific function: The Nitrate Reductase Enzyme Complex Allows E.Coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Gamma Chain Is A Membrane-Embedded Heme-Iron Unit Resembling Cytochrome B, Which Transfers Electrons From Quinones To The Beta Subunit.

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=225, Percent_Identity=30.6666666666667, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787738, Length=229, Percent_Identity=30.1310043668122, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 24563; Mature: 24431

Theoretical pI: Translated: 12.17; Mature: 12.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTFLFAVFPYLATVFAVGGAIYRRRALGGSVSARSSQLIEGRALRWGSIAWHHGILAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHH
LAAHLLAAVAPGAWGRLLGAPARLYALEITGMALAMLAVFGIALLLVRRTALARLTTPMD
HHHHHHHHHCCCHHHHHHCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
WVVLSLLLLQAATGLWIAYALRWGGLWYLHTAAPWLASLATLSPRVDAMAVLPPLVKVHV
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VNAFLLLALVPVSRLVHATSLPLTYLWRAPQRVVWRRAPSAASHDAR
HHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SDTFLFAVFPYLATVFAVGGAIYRRRALGGSVSARSSQLIEGRALRWGSIAWHHGILAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHH
LAAHLLAAVAPGAWGRLLGAPARLYALEITGMALAMLAVFGIALLLVRRTALARLTTPMD
HHHHHHHHHCCCHHHHHHCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
WVVLSLLLLQAATGLWIAYALRWGGLWYLHTAAPWLASLATLSPRVDAMAVLPPLVKVHV
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VNAFLLLALVPVSRLVHATSLPLTYLWRAPQRVVWRRAPSAASHDAR
HHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 9384377; 7557333 [H]