Click here to switch to the map view.

The map label for this gene is dnaG [H]

Identifier: 220916245

GI number: 220916245

Start: 1271242

End: 1272606

Strand: Direct

Name: dnaG [H]

Synonym: A2cp1_1138

Alternate gene names: 220916245

Gene position: 1271242-1272606 (Clockwise)

Preceding gene: 220916244

Following gene: 220916246

Centisome position: 25.28

GC content: 71.06

Gene sequence:

>1365_bases
ATGATCTCGCCCGACATCATCGAGGAGGTGAAGCGGCTTGCCGACCCCGTCGTCCTCATCGGCGCCCGGGTGAGGCTCTG
GAAGAGCGGCATCGCGTACGCGGGCTCCTGCCCGTTCCATGAGGAGCGGCAAGCGAGCTTCCGCGTCTATCCGAAGGACA
AGCACTTCGTCTGCTACGGGTGCGGCGCGAGCGGCGACATCTTCCAGTTCTTCCAGCGTGCGGACGGGAAGGCCTTCCCG
ACGGTGGTCCGCGAGCTCGCGGCGTCGCTCGGCATCGTCATCCCGCCCGAGCGGCCCGCGACGGCACAGGAGCAGCGAGA
GCGCGGCGAGCGCGCCTCGCTCCTCGCCGCCTGTGAGGCGGCGATGAGCCACTGGCAGCGGAACCTCTGGGGGAGCGAGG
GCGAGAAGGCGCGCGAGTATCTCGCGGAGCGGCGGGTGACCGACGAGGCCGCGCGCGCGTTCCGACTCGGGTTCGCCCGT
CCGGACTGGCACGACCTCGAGCGTGCGCTCGGCGCGGCGAAGATCGCGCACGCGGTCCAGCACGCCGCCGGGGTGATCAC
GTCGCGAGAGGTGCCGGGGAAGGGCGTTCGGTACCACGACCGGTTCCGCGATCGAGTCGTCTTCCCGATCGAAGACATCC
AGGGCCGCGTCACCGGATTCGGAGCCCGAACGCTGGGCGCCGAGCCGGAGGCGAAGTACCTGAACGGCCCGGAGACGCCG
CTCTTCAAGAAGTCTCGCGTGCTGTTCGGGCTGCGCCAGGCGCGAGATGTGATCCGAAGCACCGGGAGAGCGCTCCTGGT
CGAGGGCTACTTCGATGTCATCGCGCTGCACCAGGCGGGCTTCACCTCCGCCGTGGCCGCCTGCGGCACGACGCTCTCGC
GGGAGCAGGTCGAGCAGCTCGCCGGCGCGGGGTGCATGGAGCTCGTCCTCCTGTTCGACGGCGATGAGGGGGGCGCGCTC
GCGGCAGCGCGGGCGGCGCGTGTGCTTCTCCAGGCGAACCTGAGCGCGACCGTGGCGCGGGTACCGACCTCGAGCCACGG
CGGCCAGAGCGACCCTGACGTGCTCGTCGCGCGCTCGGGCCGTCGCGGCATGGAGGAGGTCCTTGCTGCGGCGAAGCCAT
TGACCGAGTTCCTCATCGATGACGCCGTCCAGCGGCACGCGGATGGCATTGGCGTCCAGGCGCCTGTCGAGCACAAGCTC
GCGGTGCTCCGCGCGGTGATGCCGTTCGTGCTCGCGACGCCAGATGGTCTCCCGCGGGCGACGTTCGAGCGAGCCGTCGC
GCGCCGGCTGGGGATCGACATCGGTCCGCTGCGGCAGGAGCTGCAGAGGGCGGCCCGCACCGAGCGGCCAGGGAGCCATC
CATGA

Upstream 100 bases:

>100_bases
AGGCTCTCGCGGCGGAGTGTGGCGAGGCTCCGGAGCAGTGGCTGCCCCGGTTCTGGGAGCGGCTCGGCGTGCCCGTCCAA
CCGCTCGAGCAGGAGACGCC

Downstream 100 bases:

>100_bases
AACACCTCCGAACGTTGGTAGTCGCCGGAGGCTTCGCGTTCGCGACCGCGGCGCACGCGCAGGGCATCCCGGTGTTCGAC
TCGTCGAGCTTCGGTCAGAT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MISPDIIEEVKRLADPVVLIGARVRLWKSGIAYAGSCPFHEERQASFRVYPKDKHFVCYGCGASGDIFQFFQRADGKAFP
TVVRELAASLGIVIPPERPATAQEQRERGERASLLAACEAAMSHWQRNLWGSEGEKAREYLAERRVTDEAARAFRLGFAR
PDWHDLERALGAAKIAHAVQHAAGVITSREVPGKGVRYHDRFRDRVVFPIEDIQGRVTGFGARTLGAEPEAKYLNGPETP
LFKKSRVLFGLRQARDVIRSTGRALLVEGYFDVIALHQAGFTSAVAACGTTLSREQVEQLAGAGCMELVLLFDGDEGGAL
AAARAARVLLQANLSATVARVPTSSHGGQSDPDVLVARSGRRGMEEVLAAAKPLTEFLIDDAVQRHADGIGVQAPVEHKL
AVLRAVMPFVLATPDGLPRATFERAVARRLGIDIGPLRQELQRAARTERPGSHP

Sequences:

>Translated_454_residues
MISPDIIEEVKRLADPVVLIGARVRLWKSGIAYAGSCPFHEERQASFRVYPKDKHFVCYGCGASGDIFQFFQRADGKAFP
TVVRELAASLGIVIPPERPATAQEQRERGERASLLAACEAAMSHWQRNLWGSEGEKAREYLAERRVTDEAARAFRLGFAR
PDWHDLERALGAAKIAHAVQHAAGVITSREVPGKGVRYHDRFRDRVVFPIEDIQGRVTGFGARTLGAEPEAKYLNGPETP
LFKKSRVLFGLRQARDVIRSTGRALLVEGYFDVIALHQAGFTSAVAACGTTLSREQVEQLAGAGCMELVLLFDGDEGGAL
AAARAARVLLQANLSATVARVPTSSHGGQSDPDVLVARSGRRGMEEVLAAAKPLTEFLIDDAVQRHADGIGVQAPVEHKL
AVLRAVMPFVLATPDGLPRATFERAVARRLGIDIGPLRQELQRAARTERPGSHP
>Mature_454_residues
MISPDIIEEVKRLADPVVLIGARVRLWKSGIAYAGSCPFHEERQASFRVYPKDKHFVCYGCGASGDIFQFFQRADGKAFP
TVVRELAASLGIVIPPERPATAQEQRERGERASLLAACEAAMSHWQRNLWGSEGEKAREYLAERRVTDEAARAFRLGFAR
PDWHDLERALGAAKIAHAVQHAAGVITSREVPGKGVRYHDRFRDRVVFPIEDIQGRVTGFGARTLGAEPEAKYLNGPETP
LFKKSRVLFGLRQARDVIRSTGRALLVEGYFDVIALHQAGFTSAVAACGTTLSREQVEQLAGAGCMELVLLFDGDEGGAL
AAARAARVLLQANLSATVARVPTSSHGGQSDPDVLVARSGRRGMEEVLAAAKPLTEFLIDDAVQRHADGIGVQAPVEHKL
AVLRAVMPFVLATPDGLPRATFERAVARRLGIDIGPLRQELQRAARTERPGSHP

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=444, Percent_Identity=33.1081081081081, Blast_Score=218, Evalue=4e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 49427; Mature: 49427

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISPDIIEEVKRLADPVVLIGARVRLWKSGIAYAGSCPFHEERQASFRVYPKDKHFVCYG
CCCHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCEEEEE
CGASGDIFQFFQRADGKAFPTVVRELAASLGIVIPPERPATAQEQRERGERASLLAACEA
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCEEECCCCCCCHHHHHHCCHHHHHHHHHHH
AMSHWQRNLWGSEGEKAREYLAERRVTDEAARAFRLGFARPDWHDLERALGAAKIAHAVQ
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
HAAGVITSREVPGKGVRYHDRFRDRVVFPIEDIQGRVTGFGARTLGAEPEAKYLNGPETP
HHHHHHCCCCCCCCCCCHHHHHHCCEEEEHHHHCCCHHCCCCHHCCCCCCHHCCCCCCCC
LFKKSRVLFGLRQARDVIRSTGRALLVEGYFDVIALHQAGFTSAVAACGTTLSREQVEQL
CHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHH
AGAGCMELVLLFDGDEGGALAAARAARVLLQANLSATVARVPTSSHGGQSDPDVLVARSG
HCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCEEEECCC
RRGMEEVLAAAKPLTEFLIDDAVQRHADGIGVQAPVEHKLAVLRAVMPFVLATPDGLPRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
TFERAVARRLGIDIGPLRQELQRAARTERPGSHP
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MISPDIIEEVKRLADPVVLIGARVRLWKSGIAYAGSCPFHEERQASFRVYPKDKHFVCYG
CCCHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCEEEEE
CGASGDIFQFFQRADGKAFPTVVRELAASLGIVIPPERPATAQEQRERGERASLLAACEA
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCEEECCCCCCCHHHHHHCCHHHHHHHHHHH
AMSHWQRNLWGSEGEKAREYLAERRVTDEAARAFRLGFARPDWHDLERALGAAKIAHAVQ
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
HAAGVITSREVPGKGVRYHDRFRDRVVFPIEDIQGRVTGFGARTLGAEPEAKYLNGPETP
HHHHHHCCCCCCCCCCCHHHHHHCCEEEEHHHHCCCHHCCCCHHCCCCCCHHCCCCCCCC
LFKKSRVLFGLRQARDVIRSTGRALLVEGYFDVIALHQAGFTSAVAACGTTLSREQVEQL
CHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHH
AGAGCMELVLLFDGDEGGALAAARAARVLLQANLSATVARVPTSSHGGQSDPDVLVARSG
HCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCEEEECCC
RRGMEEVLAAAKPLTEFLIDDAVQRHADGIGVQAPVEHKLAVLRAVMPFVLATPDGLPRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
TFERAVARRLGIDIGPLRQELQRAARTERPGSHP
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA