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The map label for this gene is virB4 [H]

Identifier: 220916244

GI number: 220916244

Start: 1268795

End: 1271245

Strand: Direct

Name: virB4 [H]

Synonym: A2cp1_1137

Alternate gene names: 220916244

Gene position: 1268795-1271245 (Clockwise)

Preceding gene: 220916243

Following gene: 220916245

Centisome position: 25.23

GC content: 70.01

Gene sequence:

>2451_bases
ATGGGCCGGGTGAACCTGCTGCAGGCGAGCCGGGGCGAGTTGCCGGCCACCGCCTTCGTTCCGTACTCGCACCACGTCAC
GCCGACGATCGTGGCGACGCGCGGAGGCGAGTACCTCTCCGCGTGGAAGCTCGGCGGGCGCGCGCACGAAGCGGCGTCCG
CCGGCGAGCAGGCGGAGTGGGTGGCGGACCTGAACAACGCCTGGCGCGGCCTCGCCGAGGCCGGCGTGGCGTTCTGGTCC
CACGTCGTCCGGCGCCGCGCGGATGCCTTCCCGCGGAGCGCCTTCGACGGGCACTTCTGCCGGCGGCTCGACGCCGCCTA
TGCGGCGACCCTCGCCGGCGCGCGGTTCATGGTGAACGAGCTGTACCTCACGCCGGTCGTCCGGACGGTGGGCGACGAGG
TCCTGGAGGCGCTCGGCCGCCGCGAGAAGGAGGACGTCCCGGCGAAGCTCCGGCGGCAGCAGGACGGCATCGCGAAGCTC
GACGACCTCAACCGGGCGCTCGGCTCGACGCTCCGGCGCTACGGCGCGGAGCTGCTCCGGACGTACGAGGGCGAGGGAGG
GAAGCTCTTCTCGGCCCCGCTCGAGGCGCTGGGGCAGCTCGTGAACGGCGAGCGCCTACCGATGCCGGTCTGCCGCGACC
GCTTCGGCGACTACCTCGTCGTGAACCGGCCGCTCTTCGGCTGGCACGGCGAGGTCGGCGAGCTCCGGCTCGTGGACCGC
GTCCGACGGTTCGGGATGCTCGAGATCGTCGAGTACGACGGGAAGGGTACTACCCCGGGCGACCTCGATGGACTCCTCCG
GGCCGAGTTCGAGTTCATCCTGACGCAGAGCTTCGCCGCGCTCTCGAAGCACGCCGCGAAGGGATTCCTGGAGCGCCACA
AGCAGCGGCTCCTCGACGCCAAGGACGTCGCGAGGAGCCAAGTCGCCGAGATCGACTTCGCACTCGACCAGCTCATGTCG
GGGTCGTTCGTCGTGGGCGAGCACCACGCGACGCTCCTCGCCTTCGGCGAGAGCGCCGACGAGGTGCGCGATCACTTGGC
CTGGGCCAGGTCCGAGCTCCTCGACCGCGGCATCGTGGCGAAGCCGGTGGACCTCGCGCTCGAGGCGGGGTTCTGGGCGC
AGCTGCCGGGGAACCTCCGCTGGAGGCCCCGGCCGATGGCGATCACCTCGCAGAACTTCCTCTGCTTCTCGCCGTTCCAC
AACTTCATGACCGGCAAGGCGGCCGGGAACCCCTGGGGTCCTGCGGTCACGGTTCTCCGGACCGCGAGCGGGACGCCGCT
CTACTTCAACTTCCACGCGTCGCCAGCCGGCGAGGACTCCGAGGGCGAACGGCTTCTCGGGAACACGCTCATCCTCGGCC
AGTCGAGCGCGGGCAAGACCGTGCTCCTCGGCTTCCTCCTCGCACAGGCCCAGAAGTACCGGCCCACTGTGGTCGCCTTC
GACAAGGACCGGGGCCTCGAGATCGCGATCCGCGCCATGGGCGGCTGCTACCTCCCGCTCAAGAGCGGTGAACCGACCGG
CTGGAACCCGGTTCAGCTCGAGCCAACGAGCGCGAACCTCCTCTTCCTGAAGGGGCTCGTGAGGGCGCTCGTGTCGGCGA
ACGGGAGCGAGGTCACGCACGAGGACGAGGTGTGGATCGATCGCTCGGTCGACACGGTGATGACGCTCATCGACCGGAGG
CATCGCCGGCTGTCGCTCCTCCTCGAGTGTCTGCCCGATCCCCTGAACGAGGACGGCGCGCATCCGAGCGTGGCGGCGCG
TCTCCGACGCTGGTGCGAGGGTGGGCCGTACGGCTGGGTGTTCGACAACGCCGAGGACGTGCTCGATCTCGGAACGAACC
GCGTTTACGGCTTCGACATCACCGAGCTCCTCGACTGCGCCGACGCGCGAGGGCCGATGATGAAGTACCTGCTCCACCGC
ACGGAGGCGATGCTCGACGGCCGCCGCTTCATCTACGTCTTCGACGAGTGGTGGCGCGCGCTCAGCGGCGAGGACTTCGC
CGAGCTCACGAAGAACAAGGGGAAGACGATCCGAAAACAGGACGGGTTCCTCGTCCTGTGCACGCAGGAGCCGGACGACG
CGCTCCAGAGCCCCGTGGGCAAGTCGGTCATCCAGCAGTGCGCGACGCTGCTGCTCCTCCGGAATCCGAACGCCACGCGC
GAGGACTACGTCGAGGGTCTGAAGCTTAGCGAGCCCGAGTACGAGCTCGTGCGGTCCCTTCCGGAGCAGAGCCGCCGGTT
CCTCGTGAAGCAGGGTGGCGGCTCGGCGGTGGCGGAGCTCGATCTGTCGGCCATGCCGGAGGCGCTCGCCGTGCTCTCCG
GCACGCCGGACCGGGCCCGGCTCGTCGAGGCTCTCGCGGCGGAGTGTGGCGAGGCTCCGGAGCAGTGGCTGCCCCGGTTC
TGGGAGCGGCTCGGCGTGCCCGTCCAACCGCTCGAGCAGGAGACGCCATGA

Upstream 100 bases:

>100_bases
TGCGCCTCGGCGCTCGGCGCGGCGTCCACGATCTCTGGGGCGCCTCGGCCTACGCGACCTCCGACCGCCGTTTCCGGAGC
TGGGAAGGAAGGAGCAGCCG

Downstream 100 bases:

>100_bases
TCTCGCCCGACATCATCGAGGAGGTGAAGCGGCTTGCCGACCCCGTCGTCCTCATCGGCGCCCGGGTGAGGCTCTGGAAG
AGCGGCATCGCGTACGCGGG

Product: type IV secretion/conjugal transfer ATPase, VirB4 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 816; Mature: 815

Protein sequence:

>816_residues
MGRVNLLQASRGELPATAFVPYSHHVTPTIVATRGGEYLSAWKLGGRAHEAASAGEQAEWVADLNNAWRGLAEAGVAFWS
HVVRRRADAFPRSAFDGHFCRRLDAAYAATLAGARFMVNELYLTPVVRTVGDEVLEALGRREKEDVPAKLRRQQDGIAKL
DDLNRALGSTLRRYGAELLRTYEGEGGKLFSAPLEALGQLVNGERLPMPVCRDRFGDYLVVNRPLFGWHGEVGELRLVDR
VRRFGMLEIVEYDGKGTTPGDLDGLLRAEFEFILTQSFAALSKHAAKGFLERHKQRLLDAKDVARSQVAEIDFALDQLMS
GSFVVGEHHATLLAFGESADEVRDHLAWARSELLDRGIVAKPVDLALEAGFWAQLPGNLRWRPRPMAITSQNFLCFSPFH
NFMTGKAAGNPWGPAVTVLRTASGTPLYFNFHASPAGEDSEGERLLGNTLILGQSSAGKTVLLGFLLAQAQKYRPTVVAF
DKDRGLEIAIRAMGGCYLPLKSGEPTGWNPVQLEPTSANLLFLKGLVRALVSANGSEVTHEDEVWIDRSVDTVMTLIDRR
HRRLSLLLECLPDPLNEDGAHPSVAARLRRWCEGGPYGWVFDNAEDVLDLGTNRVYGFDITELLDCADARGPMMKYLLHR
TEAMLDGRRFIYVFDEWWRALSGEDFAELTKNKGKTIRKQDGFLVLCTQEPDDALQSPVGKSVIQQCATLLLLRNPNATR
EDYVEGLKLSEPEYELVRSLPEQSRRFLVKQGGGSAVAELDLSAMPEALAVLSGTPDRARLVEALAAECGEAPEQWLPRF
WERLGVPVQPLEQETP

Sequences:

>Translated_816_residues
MGRVNLLQASRGELPATAFVPYSHHVTPTIVATRGGEYLSAWKLGGRAHEAASAGEQAEWVADLNNAWRGLAEAGVAFWS
HVVRRRADAFPRSAFDGHFCRRLDAAYAATLAGARFMVNELYLTPVVRTVGDEVLEALGRREKEDVPAKLRRQQDGIAKL
DDLNRALGSTLRRYGAELLRTYEGEGGKLFSAPLEALGQLVNGERLPMPVCRDRFGDYLVVNRPLFGWHGEVGELRLVDR
VRRFGMLEIVEYDGKGTTPGDLDGLLRAEFEFILTQSFAALSKHAAKGFLERHKQRLLDAKDVARSQVAEIDFALDQLMS
GSFVVGEHHATLLAFGESADEVRDHLAWARSELLDRGIVAKPVDLALEAGFWAQLPGNLRWRPRPMAITSQNFLCFSPFH
NFMTGKAAGNPWGPAVTVLRTASGTPLYFNFHASPAGEDSEGERLLGNTLILGQSSAGKTVLLGFLLAQAQKYRPTVVAF
DKDRGLEIAIRAMGGCYLPLKSGEPTGWNPVQLEPTSANLLFLKGLVRALVSANGSEVTHEDEVWIDRSVDTVMTLIDRR
HRRLSLLLECLPDPLNEDGAHPSVAARLRRWCEGGPYGWVFDNAEDVLDLGTNRVYGFDITELLDCADARGPMMKYLLHR
TEAMLDGRRFIYVFDEWWRALSGEDFAELTKNKGKTIRKQDGFLVLCTQEPDDALQSPVGKSVIQQCATLLLLRNPNATR
EDYVEGLKLSEPEYELVRSLPEQSRRFLVKQGGGSAVAELDLSAMPEALAVLSGTPDRARLVEALAAECGEAPEQWLPRF
WERLGVPVQPLEQETP
>Mature_815_residues
GRVNLLQASRGELPATAFVPYSHHVTPTIVATRGGEYLSAWKLGGRAHEAASAGEQAEWVADLNNAWRGLAEAGVAFWSH
VVRRRADAFPRSAFDGHFCRRLDAAYAATLAGARFMVNELYLTPVVRTVGDEVLEALGRREKEDVPAKLRRQQDGIAKLD
DLNRALGSTLRRYGAELLRTYEGEGGKLFSAPLEALGQLVNGERLPMPVCRDRFGDYLVVNRPLFGWHGEVGELRLVDRV
RRFGMLEIVEYDGKGTTPGDLDGLLRAEFEFILTQSFAALSKHAAKGFLERHKQRLLDAKDVARSQVAEIDFALDQLMSG
SFVVGEHHATLLAFGESADEVRDHLAWARSELLDRGIVAKPVDLALEAGFWAQLPGNLRWRPRPMAITSQNFLCFSPFHN
FMTGKAAGNPWGPAVTVLRTASGTPLYFNFHASPAGEDSEGERLLGNTLILGQSSAGKTVLLGFLLAQAQKYRPTVVAFD
KDRGLEIAIRAMGGCYLPLKSGEPTGWNPVQLEPTSANLLFLKGLVRALVSANGSEVTHEDEVWIDRSVDTVMTLIDRRH
RRLSLLLECLPDPLNEDGAHPSVAARLRRWCEGGPYGWVFDNAEDVLDLGTNRVYGFDITELLDCADARGPMMKYLLHRT
EAMLDGRRFIYVFDEWWRALSGEDFAELTKNKGKTIRKQDGFLVLCTQEPDDALQSPVGKSVIQQCATLLLLRNPNATRE
DYVEGLKLSEPEYELVRSLPEQSRRFLVKQGGGSAVAELDLSAMPEALAVLSGTPDRARLVEALAAECGEAPEQWLPRFW
ERLGVPVQPLEQETP

Specific function: The virB operon is essential for intracellular survival and is not involved in the invasion process. Constitutes a major determinant of virulence in mice [H]

COG id: COG3451

COG function: function code U; Type IV secretory pathway, VirB4 components

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trbE/virB4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004346
- InterPro:   IPR018145 [H]

Pfam domain/function: PF03135 CagE_TrbE_VirB [H]

EC number: NA

Molecular weight: Translated: 90362; Mature: 90231

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRVNLLQASRGELPATAFVPYSHHVTPTIVATRGGEYLSAWKLGGRAHEAASAGEQAEW
CCCCEEEECCCCCCCCEEECCCCCCCCCEEEEECCCCCHHHHHCCCCHHHHHCCCCHHHH
VADLNNAWRGLAEAGVAFWSHVVRRRADAFPRSAFDGHFCRRLDAAYAATLAGARFMVNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LYLTPVVRTVGDEVLEALGRREKEDVPAKLRRQQDGIAKLDDLNRALGSTLRRYGAELLR
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
TYEGEGGKLFSAPLEALGQLVNGERLPMPVCRDRFGDYLVVNRPLFGWHGEVGELRLVDR
HHCCCCCEEEHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHH
VRRFGMLEIVEYDGKGTTPGDLDGLLRAEFEFILTQSFAALSKHAAKGFLERHKQRLLDA
HHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
KDVARSQVAEIDFALDQLMSGSFVVGEHHATLLAFGESADEVRDHLAWARSELLDRGIVA
HHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCC
KPVDLALEAGFWAQLPGNLRWRPRPMAITSQNFLCFSPFHNFMTGKAAGNPWGPAVTVLR
CCHHHHHCCCCCEECCCCCCCCCCCCEEECCCCEEECCHHHHHCCCCCCCCCCCCEEEEE
TASGTPLYFNFHASPAGEDSEGERLLGNTLILGQSSAGKTVLLGFLLAQAQKYRPTVVAF
ECCCCEEEEEEECCCCCCCCCCCEECCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEE
DKDRGLEIAIRAMGGCYLPLKSGEPTGWNPVQLEPTSANLLFLKGLVRALVSANGSEVTH
ECCCCCEEEEEECCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCC
EDEVWIDRSVDTVMTLIDRRHRRLSLLLECLPDPLNEDGAHPSVAARLRRWCEGGPYGWV
CCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEE
FDNAEDVLDLGTNRVYGFDITELLDCADARGPMMKYLLHRTEAMLDGRRFIYVFDEWWRA
ECCHHHHHHCCCCCEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHH
LSGEDFAELTKNKGKTIRKQDGFLVLCTQEPDDALQSPVGKSVIQQCATLLLLRNPNATR
HCCCHHHHHHHCCCCEEEECCCEEEEEECCCHHHHHCHHHHHHHHHHHHHEEECCCCCCH
EDYVEGLKLSEPEYELVRSLPEQSRRFLVKQGGGSAVAELDLSAMPEALAVLSGTPDRAR
HHHHCCCCCCCCHHHHHHHCHHHHHHEEHCCCCCCEEEECCHHHHHHHHHHHCCCCHHHH
LVEALAAECGEAPEQWLPRFWERLGVPVQPLEQETP
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
GRVNLLQASRGELPATAFVPYSHHVTPTIVATRGGEYLSAWKLGGRAHEAASAGEQAEW
CCCEEEECCCCCCCCEEECCCCCCCCCEEEEECCCCCHHHHHCCCCHHHHHCCCCHHHH
VADLNNAWRGLAEAGVAFWSHVVRRRADAFPRSAFDGHFCRRLDAAYAATLAGARFMVNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LYLTPVVRTVGDEVLEALGRREKEDVPAKLRRQQDGIAKLDDLNRALGSTLRRYGAELLR
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
TYEGEGGKLFSAPLEALGQLVNGERLPMPVCRDRFGDYLVVNRPLFGWHGEVGELRLVDR
HHCCCCCEEEHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHH
VRRFGMLEIVEYDGKGTTPGDLDGLLRAEFEFILTQSFAALSKHAAKGFLERHKQRLLDA
HHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
KDVARSQVAEIDFALDQLMSGSFVVGEHHATLLAFGESADEVRDHLAWARSELLDRGIVA
HHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCC
KPVDLALEAGFWAQLPGNLRWRPRPMAITSQNFLCFSPFHNFMTGKAAGNPWGPAVTVLR
CCHHHHHCCCCCEECCCCCCCCCCCCEEECCCCEEECCHHHHHCCCCCCCCCCCCEEEEE
TASGTPLYFNFHASPAGEDSEGERLLGNTLILGQSSAGKTVLLGFLLAQAQKYRPTVVAF
ECCCCEEEEEEECCCCCCCCCCCEECCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEE
DKDRGLEIAIRAMGGCYLPLKSGEPTGWNPVQLEPTSANLLFLKGLVRALVSANGSEVTH
ECCCCCEEEEEECCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCC
EDEVWIDRSVDTVMTLIDRRHRRLSLLLECLPDPLNEDGAHPSVAARLRRWCEGGPYGWV
CCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEE
FDNAEDVLDLGTNRVYGFDITELLDCADARGPMMKYLLHRTEAMLDGRRFIYVFDEWWRA
ECCHHHHHHCCCCCEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHH
LSGEDFAELTKNKGKTIRKQDGFLVLCTQEPDDALQSPVGKSVIQQCATLLLLRNPNATR
HCCCHHHHHHHCCCCEEEECCCEEEEEECCCHHHHHCHHHHHHHHHHHHHEEECCCCCCH
EDYVEGLKLSEPEYELVRSLPEQSRRFLVKQGGGSAVAELDLSAMPEALAVLSGTPDRAR
HHHHCCCCCCCCHHHHHHHCHHHHHHEEHCCCCCCEEEECCHHHHHHHHHHHCCCCHHHH
LVEALAAECGEAPEQWLPRFWERLGVPVQPLEQETP
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10940027 [H]