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The map label for this gene is recF

Identifier: 220915126

GI number: 220915126

Start: 3353

End: 4471

Strand: Direct

Name: recF

Synonym: A2cp1_0003

Alternate gene names: 220915126

Gene position: 3353-4471 (Clockwise)

Preceding gene: 220915125

Following gene: 220915127

Centisome position: 0.07

GC content: 75.69

Gene sequence:

>1119_bases
GTGAAGCTCCTCTCCCTCCACGTCCAGGACTTCCGGAACCTCGCCGCGGTCGAGCTCGCGCCGTCGCCGCGGGCCACCGT
GCTCCTGGGCGAGAACGGCCAGGGGAAGACCAACCTCCTCGAGGCCATCTACTTCCTCACCACGCTGAAGCCGCTCCGCG
CGGTGCGGCTCGCGGAGCTGGTCCGGTTCGGCGCCGAGCAGGGCGCGGTGGCCGGCGACTTCGAGGGGCCGGGCGGCGTG
CGCCGGGTGGCGGTGCAGGTGGCCGCCGGCGGCCGGACCGCGACGCTCGACGGCAAGGCGCTCGGCTCCGGGGCCCGCCT
CGATGACTACTTCGAGGGGCTCGCGTCCGTCTGCTTCTCGCCGGACGACCTGCTGCTCGTGAAGGCCGGGCCGGACGGGC
GCCGTCGCTTCCTGGATCGCGCCGCCTTCAACCGCTGGCCGGCGGTGCTCGGGGAGGCGCGCGAGTACGTGCGGGCGCTC
CGGGCCCGGAACGCGGCGCTCCGCGCCGGGCCGGCGGAAGTGGAGGCGAGCTTCCGCGAGCCGCTGGTGCGCGCCGGTGC
GCGCATCCTCGTCCGGCGGCGCGACCTCGTGGCGGAGCTCGCCCCGCGGCTCCAGGCCGCCTTCGCCGAGATCTCCGGGC
CGGCCGCCCCCGAGGCGCACCTCGCCTACCGCGCCGCGGGCGGGGTGGACGTCGAGCACCCGGAGGCGGAGGTGGCCGCG
CGGCTCGCCCACGCGCTCGAGGCGCGCCTGGAGCGCGATCGCGAGAAGGGCTTCACCTCGGCCGGGCCGCACATGGACGA
CCTCGTGCTCGCGCTCGGCGGCAAGGGCGCGCGGCTCTACGGGAGCCAGGGCCAGCAGCGCGCGCTCGTGCTCGCGCTCA
AGATCGCCGAGATCGAGAACCTGCGCGCGGCGCTGGGGCGGCCGCCGCTGCTGCTGCTCGACGACGTGTCCTCGGAGCTG
GATCCGGCCAAGAACCGCTTCCTGCTCGGCTACCTGGCGGCCCTGCCGGCGCAGGCGTTCCTCACCTCGACCGATCGCCG
GCTCATCGAGCCCGCCGCCGGCCCCGACACCGCCTTCTTCGAGGTGCGATCGGGGGTGGTTTCCCCGCTGGTTTCATGA

Upstream 100 bases:

>100_bases
TCCTCCCTCACATGCCTCGGGGCATGCTCGGTCGTCGCGCCTCGCGCTGCTCGCGTCTCGACCGCCTGGCTCGGTCCGGG
GATGGATTCACGCGCCCTAA

Downstream 100 bases:

>100_bases
AGAAGCTCGCGGGGTTGCGGTCCGGATAAGACGTTGCCTCCGGCGTCCGCGCCTGCTATACCCCAGGGTCGCCTCAACAC
CTGAGAACCTTTCAGAGAAA

Product: DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKLLSLHVQDFRNLAAVELAPSPRATVLLGENGQGKTNLLEAIYFLTTLKPLRAVRLAELVRFGAEQGAVAGDFEGPGGV
RRVAVQVAAGGRTATLDGKALGSGARLDDYFEGLASVCFSPDDLLLVKAGPDGRRRFLDRAAFNRWPAVLGEAREYVRAL
RARNAALRAGPAEVEASFREPLVRAGARILVRRRDLVAELAPRLQAAFAEISGPAAPEAHLAYRAAGGVDVEHPEAEVAA
RLAHALEARLERDREKGFTSAGPHMDDLVLALGGKGARLYGSQGQQRALVLALKIAEIENLRAALGRPPLLLLDDVSSEL
DPAKNRFLLGYLAALPAQAFLTSTDRRLIEPAAGPDTAFFEVRSGVVSPLVS

Sequences:

>Translated_372_residues
MKLLSLHVQDFRNLAAVELAPSPRATVLLGENGQGKTNLLEAIYFLTTLKPLRAVRLAELVRFGAEQGAVAGDFEGPGGV
RRVAVQVAAGGRTATLDGKALGSGARLDDYFEGLASVCFSPDDLLLVKAGPDGRRRFLDRAAFNRWPAVLGEAREYVRAL
RARNAALRAGPAEVEASFREPLVRAGARILVRRRDLVAELAPRLQAAFAEISGPAAPEAHLAYRAAGGVDVEHPEAEVAA
RLAHALEARLERDREKGFTSAGPHMDDLVLALGGKGARLYGSQGQQRALVLALKIAEIENLRAALGRPPLLLLDDVSSEL
DPAKNRFLLGYLAALPAQAFLTSTDRRLIEPAAGPDTAFFEVRSGVVSPLVS
>Mature_372_residues
MKLLSLHVQDFRNLAAVELAPSPRATVLLGENGQGKTNLLEAIYFLTTLKPLRAVRLAELVRFGAEQGAVAGDFEGPGGV
RRVAVQVAAGGRTATLDGKALGSGARLDDYFEGLASVCFSPDDLLLVKAGPDGRRRFLDRAAFNRWPAVLGEAREYVRAL
RARNAALRAGPAEVEASFREPLVRAGARILVRRRDLVAELAPRLQAAFAEISGPAAPEAHLAYRAAGGVDVEHPEAEVAA
RLAHALEARLERDREKGFTSAGPHMDDLVLALGGKGARLYGSQGQQRALVLALKIAEIENLRAALGRPPLLLLDDVSSEL
DPAKNRFLLGYLAALPAQAFLTSTDRRLIEPAAGPDTAFFEVRSGVVSPLVS

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=372, Percent_Identity=29.5698924731183, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_ANAD2 (B8J6Y3)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002490430.1
- ProteinModelPortal:   B8J6Y3
- SMR:   B8J6Y3
- GeneID:   7296643
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_0003
- HOGENOM:   HBG645124
- ProtClustDB:   CLSK944756
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 39707; Mature: 39707

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLSLHVQDFRNLAAVELAPSPRATVLLGENGQGKTNLLEAIYFLTTLKPLRAVRLAEL
CCEEHHHHHHHHCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VRFGAEQGAVAGDFEGPGGVRRVAVQVAAGGRTATLDGKALGSGARLDDYFEGLASVCFS
HHHCCCCCCEECCCCCCCCCEEEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCC
PDDLLLVKAGPDGRRRFLDRAAFNRWPAVLGEAREYVRALRARNAALRAGPAEVEASFRE
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHH
PLVRAGARILVRRRDLVAELAPRLQAAFAEISGPAAPEAHLAYRAAGGVDVEHPEAEVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEECCCCCCCCCHHHHHH
RLAHALEARLERDREKGFTSAGPHMDDLVLALGGKGARLYGSQGQQRALVLALKIAEIEN
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHEEECCCCCEECCCCCCCEEEEHHHHHHHHHH
LRAALGRPPLLLLDDVSSELDPAKNRFLLGYLAALPAQAFLTSTDRRLIEPAAGPDTAFF
HHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCHHHCCCCCCCCHHHH
EVRSGVVSPLVS
HHHHHHHHHCCC
>Mature Secondary Structure
MKLLSLHVQDFRNLAAVELAPSPRATVLLGENGQGKTNLLEAIYFLTTLKPLRAVRLAEL
CCEEHHHHHHHHCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VRFGAEQGAVAGDFEGPGGVRRVAVQVAAGGRTATLDGKALGSGARLDDYFEGLASVCFS
HHHCCCCCCEECCCCCCCCCEEEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCC
PDDLLLVKAGPDGRRRFLDRAAFNRWPAVLGEAREYVRALRARNAALRAGPAEVEASFRE
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHH
PLVRAGARILVRRRDLVAELAPRLQAAFAEISGPAAPEAHLAYRAAGGVDVEHPEAEVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEECCCCCCCCCHHHHHH
RLAHALEARLERDREKGFTSAGPHMDDLVLALGGKGARLYGSQGQQRALVLALKIAEIEN
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHEEECCCCCEECCCCCCCEEEEHHHHHHHHHH
LRAALGRPPLLLLDDVSSELDPAKNRFLLGYLAALPAQAFLTSTDRRLIEPAAGPDTAFF
HHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCHHHCCCCCCCCHHHH
EVRSGVVSPLVS
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA