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The map label for this gene is dnaN [H]

Identifier: 220915125

GI number: 220915125

Start: 2042

End: 3169

Strand: Direct

Name: dnaN [H]

Synonym: A2cp1_0002

Alternate gene names: 220915125

Gene position: 2042-3169 (Clockwise)

Preceding gene: 220915124

Following gene: 220915126

Centisome position: 0.04

GC content: 68.44

Gene sequence:

>1128_bases
ATGGAACTGAAGATCGGCGCCCAGGAGCTCGCGAAGGCGCTCGGCCGCTCGCAGGGCATCGTCGAGAAGAAGAGCACGAT
GCCGATCCTCTCCCACGTCCTGCTCGAGGCGAAGAAGGGCGACCAGCTCGTCGTCTCCGCCACCGACCTCGACCTCGCCG
TCTCCTCCGAGCACGCCTGCGAGGTGCTGAAGGAGGGCGCGGTGGCCGTCTCGGCCCGCCACCTCTACGAGATCGTCCGC
GCCCTGCCCGAGCAGACCGTCACGCTCAAGAAGGCGCACAACAACTACCTCGAGGTGAAGAGCGGCCCGTCCGAGTTCCG
GATCGTCGGCCTGCCCGCCGAGGACTTCCCGGCGCTGCCCCGCTTCGACAAGGTTCCGTTCGCCCCGGTGGACCCGGCGC
TCCTGCTCGAGATGATCGAGCGGACCTTCTTCGCCGTCTCGAACGACGAGACCCGCTACAACCTGAACGGCGTGTACTTC
GAGCCCGCAGGCGACGCGCTCCGCCTGGTCGCGACCGACGGCCACCGGCTCTCGCTCTCCGAGCGCGCGGTGGGCGCCAC
CTTCGGCCTGAAGCGCGGCGTGATCCTGCCGAAGAAGGGGCTCGCCGAGCTGAAGAAGCTGCTCGCCGAGGCGGCCGAGT
CCGGCGAGGAGCAGCCGGAGGCGAAGCTCGGCTTCGTCGAGAACAGCGCCATCTTCCGCCGGCCGAACGTGGTGCTGGTG
ATGCGGCTCATCGAGGGGCTGTTCCCGGACTACAAGCAGGTCATCCCGAAGGCCGGCGAGAAGGTCTTCAAGGTGGGCCG
CGGCCGCTTCCTCGAGACGCTGCGGCGCATCTCGCTCCTCTCCTCCGACAAGGCGCACGCGGTGAAGCTGGACCTCTCGA
AGGACCTGCTCCGCGTCCTCTCGCAGAACCCGGACCTGGGCGAGGCGAAGGAGGAGGTGCCGGTGGAGTACGCGGGCGAG
CCGCTCAAGATCGGCTTCAACGCGCGCTACATCATGGAGGTCCTCCAGGCGGTGAAGTCGAACGAGGTGGTGTTCGAGCT
CGCCGACGACCTCTCCCCCGGCGTCCTGAAGGGCGGCGAGGAGGCGGACCAGGGCTTCACCGCGGTCGTGATGCCCATGC
GCATCTGA

Upstream 100 bases:

>100_bases
TCCGGGGAAGAAACTCGAGCAGGATCGGGGCGCTCGGTCGCTTTTCCACATCGATCCTCCCCCTGCTTCGACGACGGATC
TCAAAGAAGAGGATCATCCC

Downstream 100 bases:

>100_bases
CGGTCCGTGTCCCGCCTGAGGGCACGTGAACCGATCCCCTCCCGTCGCCGCGGCGGCCGATCCGCGTCGCATCGTGTCGT
TGCTCCTCCCTCACATGCCT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MELKIGAQELAKALGRSQGIVEKKSTMPILSHVLLEAKKGDQLVVSATDLDLAVSSEHACEVLKEGAVAVSARHLYEIVR
ALPEQTVTLKKAHNNYLEVKSGPSEFRIVGLPAEDFPALPRFDKVPFAPVDPALLLEMIERTFFAVSNDETRYNLNGVYF
EPAGDALRLVATDGHRLSLSERAVGATFGLKRGVILPKKGLAELKKLLAEAAESGEEQPEAKLGFVENSAIFRRPNVVLV
MRLIEGLFPDYKQVIPKAGEKVFKVGRGRFLETLRRISLLSSDKAHAVKLDLSKDLLRVLSQNPDLGEAKEEVPVEYAGE
PLKIGFNARYIMEVLQAVKSNEVVFELADDLSPGVLKGGEEADQGFTAVVMPMRI

Sequences:

>Translated_375_residues
MELKIGAQELAKALGRSQGIVEKKSTMPILSHVLLEAKKGDQLVVSATDLDLAVSSEHACEVLKEGAVAVSARHLYEIVR
ALPEQTVTLKKAHNNYLEVKSGPSEFRIVGLPAEDFPALPRFDKVPFAPVDPALLLEMIERTFFAVSNDETRYNLNGVYF
EPAGDALRLVATDGHRLSLSERAVGATFGLKRGVILPKKGLAELKKLLAEAAESGEEQPEAKLGFVENSAIFRRPNVVLV
MRLIEGLFPDYKQVIPKAGEKVFKVGRGRFLETLRRISLLSSDKAHAVKLDLSKDLLRVLSQNPDLGEAKEEVPVEYAGE
PLKIGFNARYIMEVLQAVKSNEVVFELADDLSPGVLKGGEEADQGFTAVVMPMRI
>Mature_375_residues
MELKIGAQELAKALGRSQGIVEKKSTMPILSHVLLEAKKGDQLVVSATDLDLAVSSEHACEVLKEGAVAVSARHLYEIVR
ALPEQTVTLKKAHNNYLEVKSGPSEFRIVGLPAEDFPALPRFDKVPFAPVDPALLLEMIERTFFAVSNDETRYNLNGVYF
EPAGDALRLVATDGHRLSLSERAVGATFGLKRGVILPKKGLAELKKLLAEAAESGEEQPEAKLGFVENSAIFRRPNVVLV
MRLIEGLFPDYKQVIPKAGEKVFKVGRGRFLETLRRISLLSSDKAHAVKLDLSKDLLRVLSQNPDLGEAKEEVPVEYAGE
PLKIGFNARYIMEVLQAVKSNEVVFELADDLSPGVLKGGEEADQGFTAVVMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=376, Percent_Identity=32.7127659574468, Blast_Score=196, Evalue=3e-51,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41077; Mature: 41077

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKIGAQELAKALGRSQGIVEKKSTMPILSHVLLEAKKGDQLVVSATDLDLAVSSEHAC
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCHHHH
EVLKEGAVAVSARHLYEIVRALPEQTVTLKKAHNNYLEVKSGPSEFRIVGLPAEDFPALP
HHHHCCCHHHHHHHHHHHHHHCCHHHEEHHHHCCCEEEECCCCCCEEEEECCCCCCCCCC
RFDKVPFAPVDPALLLEMIERTFFAVSNDETRYNLNGVYFEPAGDALRLVATDGHRLSLS
CCCCCCCCCCCHHHHHHHHHHHHEEECCCCCEEECCEEEECCCCCEEEEEECCCCEEECH
ERAVGATFGLKRGVILPKKGLAELKKLLAEAAESGEEQPEAKLGFVENSAIFRRPNVVLV
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCEEECCCEEECCCHHHH
MRLIEGLFPDYKQVIPKAGEKVFKVGRGRFLETLRRISLLSSDKAHAVKLDLSKDLLRVL
HHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHH
SQNPDLGEAKEEVPVEYAGEPLKIGFNARYIMEVLQAVKSNEVVFELADDLSPGVLKGGE
HCCCCCCCHHHHCCHHHCCCCEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
EADQGFTAVVMPMRI
HHCCCCEEEEEEECC
>Mature Secondary Structure
MELKIGAQELAKALGRSQGIVEKKSTMPILSHVLLEAKKGDQLVVSATDLDLAVSSEHAC
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCHHHH
EVLKEGAVAVSARHLYEIVRALPEQTVTLKKAHNNYLEVKSGPSEFRIVGLPAEDFPALP
HHHHCCCHHHHHHHHHHHHHHCCHHHEEHHHHCCCEEEECCCCCCEEEEECCCCCCCCCC
RFDKVPFAPVDPALLLEMIERTFFAVSNDETRYNLNGVYFEPAGDALRLVATDGHRLSLS
CCCCCCCCCCCHHHHHHHHHHHHEEECCCCCEEECCEEEECCCCCEEEEEECCCCEEECH
ERAVGATFGLKRGVILPKKGLAELKKLLAEAAESGEEQPEAKLGFVENSAIFRRPNVVLV
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCEEECCCEEECCCHHHH
MRLIEGLFPDYKQVIPKAGEKVFKVGRGRFLETLRRISLLSSDKAHAVKLDLSKDLLRVL
HHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHH
SQNPDLGEAKEEVPVEYAGEPLKIGFNARYIMEVLQAVKSNEVVFELADDLSPGVLKGGE
HCCCCCCCHHHHCCHHHCCCCEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
EADQGFTAVVMPMRI
HHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]