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The map label for this gene is murE [H]

Identifier: 220904379

GI number: 220904379

Start: 1300088

End: 1301542

Strand: Direct

Name: murE [H]

Synonym: Ddes_1108

Alternate gene names: 220904379

Gene position: 1300088-1301542 (Clockwise)

Preceding gene: 220904378

Following gene: 220904380

Centisome position: 45.25

GC content: 58.97

Gene sequence:

>1455_bases
ATGAATCGGGAATTTGCGGCACTGCTGGATGAGTGCGGACGCGGCGAGATTGAAGTGCGCGCAGACTCGCGTCAGGTATG
TCCCGGCGATATTTTTGTGGCTGTACCTGGCGTCAGGGAAGACGGCGCCCAGTTTATCCCTGCTGCCGCTGAAGCCGGGG
CGGGAGTCATTGTTTGCCGGGCCGACAGTGAAGAGGCTGCAGCTGCAGCGACCGCCGGGTGCAGGGTCGTCCACCATGCC
GATCCGCGTGAGGCGCTCTGGCGGCTGGCACAGGCCCGCTGGCATACAGATACCTTGCCTATCCGTATTGTGGGTGTTAC
AGGCACCAACGGCAAAACCACCTGCACCTATCTGCTTGAACGCCTTTTCGCCCAGGCAGGGCATAAGCTCGGCGTGATGG
GAACAGTGAGCTATCGCTGGCCCGGCCATGTTGAGGCGGCCCCCCTGACCACTCCTGATGCCTTGAGCGTGCATGCCATG
CTGGCCGCAATGGCCCGCGATGGCGTGGACGTGGCCGTGATGGAAGTCTCCTCTCATGCCATAGACCAGCAGCGGGTGTG
CGGTGTGCCGTTTTCCGGCGCGGCCTTCACCAACCTGAGCCAGGATCATCTCGATTATCATCGGGATATGGAAAGTTATT
TTCAGGCCAAGGCCAGGCTTTTTCTTGAGCTGCCGCGCGCCGACAAATCCATGGCCGTCAATGCTGACGATGCCTGGGGC
CGGCGCCTGCTGGAGCTGTGTCCCACGGCTCTTTCCTTCGGTCTGCAAAAGGGTGCGCCCAACAGGCGGCACCTGTGGGG
AGAGCTGCTTTCTTCCACCACAGCAGGCTGTCATCTGCGTATGAGCCTTGAAGACATGCAGTGGGAGCTACGCTCTCCCC
TTGTGGGGGCTTTCAATGCTTCCAACCTGCTGGCTGTGCAGGCTGTGGCTCTGGAAATGGGAATTGATCCTGAAGCCTTC
AAGGCTCTTGAAAGCTTTACGGGGGTCAGCGGCAGGCTGGAGCGGGTGGAAAATCCGCAAGGACTCAATGTTTTTGTGGA
TTATGCCCATACTCCCGATGCACTTGAGAATGTTCTCAAGGCCCTGCGCGATGCAGGCTTTGAACGCGTGATCACTGTTT
TTGGCTGTGGCGGTAACCGTGACCGCAGCAAGAGACCCATCATGGGAGAAGCTGTGGCCCGCTGGTCGGACGTGGCCGTG
CTGACCTCGGACAATCCGCGGTTTGAAGATCCGGAGGCCATACTCAAGGATGTGTTGCCTGGCCTTGCAGATGCCAAAGA
GGTTGTCGTCGAGGTTGACCGTCGCGAGGCCACCACCCGGGCTTTGGGCATGCTTGGCAAAAAAGATGCGTTGCTTATTG
CCGGCAAAGGGCATGAAGACTACCAGATTATTCAGGGGGTTAAACACCATTACAGCGATCAGGAAGTGGTGCGGGAGATA
CTCCGGTGCGCATAA

Upstream 100 bases:

>100_bases
GATGCAATCTCTGATGATGCCGACTGGTTCGGCGCAGACTGGTCTGCTCCACCGCAAGGCGACGGGCTTAAAGCGAAATT
GCTCTACAAAGAGGTTGATT

Downstream 100 bases:

>100_bases
CCATTAATGAGGCGGCGGTTTGCCTGGGCTTGCCCGGAACTTTGCCGGATGCGGAAAATACCGTCCTGACTTCGGTCGTG
ACAGACAGTCGTGACGCAGG

Product: UDP-N-acetylmuramyl-tripeptide synthetase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MNREFAALLDECGRGEIEVRADSRQVCPGDIFVAVPGVREDGAQFIPAAAEAGAGVIVCRADSEEAAAAATAGCRVVHHA
DPREALWRLAQARWHTDTLPIRIVGVTGTNGKTTCTYLLERLFAQAGHKLGVMGTVSYRWPGHVEAAPLTTPDALSVHAM
LAAMARDGVDVAVMEVSSHAIDQQRVCGVPFSGAAFTNLSQDHLDYHRDMESYFQAKARLFLELPRADKSMAVNADDAWG
RRLLELCPTALSFGLQKGAPNRRHLWGELLSSTTAGCHLRMSLEDMQWELRSPLVGAFNASNLLAVQAVALEMGIDPEAF
KALESFTGVSGRLERVENPQGLNVFVDYAHTPDALENVLKALRDAGFERVITVFGCGGNRDRSKRPIMGEAVARWSDVAV
LTSDNPRFEDPEAILKDVLPGLADAKEVVVEVDRREATTRALGMLGKKDALLIAGKGHEDYQIIQGVKHHYSDQEVVREI
LRCA

Sequences:

>Translated_484_residues
MNREFAALLDECGRGEIEVRADSRQVCPGDIFVAVPGVREDGAQFIPAAAEAGAGVIVCRADSEEAAAAATAGCRVVHHA
DPREALWRLAQARWHTDTLPIRIVGVTGTNGKTTCTYLLERLFAQAGHKLGVMGTVSYRWPGHVEAAPLTTPDALSVHAM
LAAMARDGVDVAVMEVSSHAIDQQRVCGVPFSGAAFTNLSQDHLDYHRDMESYFQAKARLFLELPRADKSMAVNADDAWG
RRLLELCPTALSFGLQKGAPNRRHLWGELLSSTTAGCHLRMSLEDMQWELRSPLVGAFNASNLLAVQAVALEMGIDPEAF
KALESFTGVSGRLERVENPQGLNVFVDYAHTPDALENVLKALRDAGFERVITVFGCGGNRDRSKRPIMGEAVARWSDVAV
LTSDNPRFEDPEAILKDVLPGLADAKEVVVEVDRREATTRALGMLGKKDALLIAGKGHEDYQIIQGVKHHYSDQEVVREI
LRCA
>Mature_484_residues
MNREFAALLDECGRGEIEVRADSRQVCPGDIFVAVPGVREDGAQFIPAAAEAGAGVIVCRADSEEAAAAATAGCRVVHHA
DPREALWRLAQARWHTDTLPIRIVGVTGTNGKTTCTYLLERLFAQAGHKLGVMGTVSYRWPGHVEAAPLTTPDALSVHAM
LAAMARDGVDVAVMEVSSHAIDQQRVCGVPFSGAAFTNLSQDHLDYHRDMESYFQAKARLFLELPRADKSMAVNADDAWG
RRLLELCPTALSFGLQKGAPNRRHLWGELLSSTTAGCHLRMSLEDMQWELRSPLVGAFNASNLLAVQAVALEMGIDPEAF
KALESFTGVSGRLERVENPQGLNVFVDYAHTPDALENVLKALRDAGFERVITVFGCGGNRDRSKRPIMGEAVARWSDVAV
LTSDNPRFEDPEAILKDVLPGLADAKEVVVEVDRREATTRALGMLGKKDALLIAGKGHEDYQIIQGVKHHYSDQEVVREI
LRCA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=478, Percent_Identity=40.3765690376569, Blast_Score=262, Evalue=3e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 52680; Mature: 52680

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNREFAALLDECGRGEIEVRADSRQVCPGDIFVAVPGVREDGAQFIPAAAEAGAGVIVCR
CCCHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCHHCCCHHCCCCCEEEEE
ADSEEAAAAATAGCRVVHHADPREALWRLAQARWHTDTLPIRIVGVTGTNGKTTCTYLLE
CCCHHHHHHHHHCCEEEECCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHH
RLFAQAGHKLGVMGTVSYRWPGHVEAAPLTTPDALSVHAMLAAMARDGVDVAVMEVSSHA
HHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHH
IDQQRVCGVPFSGAAFTNLSQDHLDYHRDMESYFQAKARLFLELPRADKSMAVNADDAWG
CCHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEECCCHHHH
RRLLELCPTALSFGLQKGAPNRRHLWGELLSSTTAGCHLRMSLEDMQWELRSPLVGAFNA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHCCCCEECCCC
SNLLAVQAVALEMGIDPEAFKALESFTGVSGRLERVENPQGLNVFVDYAHTPDALENVLK
CCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEECCCHHHHHHHHH
ALRDAGFERVITVFGCGGNRDRSKRPIMGEAVARWSDVAVLTSDNPRFEDPEAILKDVLP
HHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHC
GLADAKEVVVEVDRREATTRALGMLGKKDALLIAGKGHEDYQIIQGVKHHYSDQEVVREI
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHH
LRCA
HHCC
>Mature Secondary Structure
MNREFAALLDECGRGEIEVRADSRQVCPGDIFVAVPGVREDGAQFIPAAAEAGAGVIVCR
CCCHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCHHCCCHHCCCCCEEEEE
ADSEEAAAAATAGCRVVHHADPREALWRLAQARWHTDTLPIRIVGVTGTNGKTTCTYLLE
CCCHHHHHHHHHCCEEEECCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHH
RLFAQAGHKLGVMGTVSYRWPGHVEAAPLTTPDALSVHAMLAAMARDGVDVAVMEVSSHA
HHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHH
IDQQRVCGVPFSGAAFTNLSQDHLDYHRDMESYFQAKARLFLELPRADKSMAVNADDAWG
CCHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEECCCHHHH
RRLLELCPTALSFGLQKGAPNRRHLWGELLSSTTAGCHLRMSLEDMQWELRSPLVGAFNA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHCCCCEECCCC
SNLLAVQAVALEMGIDPEAFKALESFTGVSGRLERVENPQGLNVFVDYAHTPDALENVLK
CCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEECCCHHHHHHHHH
ALRDAGFERVITVFGCGGNRDRSKRPIMGEAVARWSDVAVLTSDNPRFEDPEAILKDVLP
HHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHC
GLADAKEVVVEVDRREATTRALGMLGKKDALLIAGKGHEDYQIIQGVKHHYSDQEVVREI
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHH
LRCA
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA