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The map label for this gene is murF [H]

Identifier: 220904380

GI number: 220904380

Start: 1301533

End: 1302951

Strand: Direct

Name: murF [H]

Synonym: Ddes_1109

Alternate gene names: 220904380

Gene position: 1301533-1302951 (Clockwise)

Preceding gene: 220904379

Following gene: 220904381

Centisome position: 45.3

GC content: 60.68

Gene sequence:

>1419_bases
GTGCGCATAACCATTAATGAGGCGGCGGTTTGCCTGGGCTTGCCCGGAACTTTGCCGGATGCGGAAAATACCGTCCTGAC
TTCGGTCGTGACAGACAGTCGTGACGCAGGGCCAGGCGCGCTTTTTGTCTGTGTACCCGGCAGCAGGGTGGACGGGCACG
ACTTTGCCGCGGCGGCTGTGAGGCAAGGCGCTGCTGCGGTGCTGGCCGCAGGGCCTTTGCCGGGCATCAATGTGCCGGTT
CTGGTGGTCCCTGATACGGTCAAGGCCCTGGGGCAACTGGCCGGGCTGTGGCGTGACAGGACGAAGGCGCGCGTGGTGGC
TGTTACCGGTACGGCAGGCAAGACGACCCTCAAGGAAGTGTTGGCCCAGGTTTTGTCCCTTGGTGGAAAGACCGCCCGCA
ATGCCATGAACCATAATAACCAGATCGGTATGCCCCGAGCGGTGCTCGGCACAGACGGCGACGAAAAGTTTTGGATTATG
GAAGCCGGAATCAGCCAGCAAGGCGACATGGATGATCTGGGTTCAGTGCTACGCCCGGATATTGCGGTTGTGCTCAACGC
CGGAACAGGCCACACTGAGGGGCTTGGCGAAAAAGGCGTGGCCTGGCACAAGGCGCGTTTGCTCAAGTACCTTGCCAAAG
GCGGTAAAGGTCTTGTCTGCGCTGATTATCCCGATCTGGTGCGTGAAGCACGAGCCACGGAGACCGACATCTGTTTCTTC
AGCGGCAGCGGCCGTGATGCGGAATTTCGCGCCGTTTATGCGGGTGCGGCTCCACAGGGGGCTGCTGTTACTCCGTCTGA
TGCCTACGGCTTGTACCGCTTATGGCTAGGGGGCAGGGAGTTTGACGTTACGGCTCCTTTCAGGGGCGCGTATGGAGCTG
AGAACGTGGCTGCCGTGGCTGCCGTCGCCCACATGCTCGGGCTGGACTTGCAGGCCATACGGCAGGGGCTGGCGCAGGCC
CGCATGCCTGCGCAACGCTTCAATCAGGTGCAGGCAGGATCGTGGCTGTTGATTGATGATACGTACAACGCCAATCCACT
GTCCATGGGCCGGATGCTGGACGCAGCGGCTGAAAGGGCGGCAGGGCGTCCTTTTGTGGCAGTGTTGGGCGCCATGCTTG
AGCTTGGCAGCCAGGCAGCCATGGAGCATGAGGCCTTGGGCCGTCGCCTGGCCGGTCTGAATCCCTCTGCGATTGTTTGG
AAGGGGCTTCATGCGGAGGATGTGCGCAAAGGTCTTACTTTGGCGGGTTATGCCGGTCCCTGGCAGGTCGTCACCGACGC
CGGGGAGTTCATGAAAACATGGCAGACTCTGCAGGATTACGGTCTGGAAAACAAGGCAGCCAAAAGTGGCGGCGTGGTTC
TGTTTAAAGGTTCGCGCAGCAACAGGCTCGAAACACTTGTGGCAGTCTTGAGCAGCTAG

Upstream 100 bases:

>100_bases
ATGCGTTGCTTATTGCCGGCAAAGGGCATGAAGACTACCAGATTATTCAGGGGGTTAAACACCATTACAGCGATCAGGAA
GTGGTGCGGGAGATACTCCG

Downstream 100 bases:

>100_bases
AGTTTTTTACGTTTGCATGACTGCTCAACAGCGGAATATTTCTGTTTTTTAGCCCGCAACTTCCTTCGTCGTAACGATGG
AAGCAGGTTGCGCCTTGCAA

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate/D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MRITINEAAVCLGLPGTLPDAENTVLTSVVTDSRDAGPGALFVCVPGSRVDGHDFAAAAVRQGAAAVLAAGPLPGINVPV
LVVPDTVKALGQLAGLWRDRTKARVVAVTGTAGKTTLKEVLAQVLSLGGKTARNAMNHNNQIGMPRAVLGTDGDEKFWIM
EAGISQQGDMDDLGSVLRPDIAVVLNAGTGHTEGLGEKGVAWHKARLLKYLAKGGKGLVCADYPDLVREARATETDICFF
SGSGRDAEFRAVYAGAAPQGAAVTPSDAYGLYRLWLGGREFDVTAPFRGAYGAENVAAVAAVAHMLGLDLQAIRQGLAQA
RMPAQRFNQVQAGSWLLIDDTYNANPLSMGRMLDAAAERAAGRPFVAVLGAMLELGSQAAMEHEALGRRLAGLNPSAIVW
KGLHAEDVRKGLTLAGYAGPWQVVTDAGEFMKTWQTLQDYGLENKAAKSGGVVLFKGSRSNRLETLVAVLSS

Sequences:

>Translated_472_residues
MRITINEAAVCLGLPGTLPDAENTVLTSVVTDSRDAGPGALFVCVPGSRVDGHDFAAAAVRQGAAAVLAAGPLPGINVPV
LVVPDTVKALGQLAGLWRDRTKARVVAVTGTAGKTTLKEVLAQVLSLGGKTARNAMNHNNQIGMPRAVLGTDGDEKFWIM
EAGISQQGDMDDLGSVLRPDIAVVLNAGTGHTEGLGEKGVAWHKARLLKYLAKGGKGLVCADYPDLVREARATETDICFF
SGSGRDAEFRAVYAGAAPQGAAVTPSDAYGLYRLWLGGREFDVTAPFRGAYGAENVAAVAAVAHMLGLDLQAIRQGLAQA
RMPAQRFNQVQAGSWLLIDDTYNANPLSMGRMLDAAAERAAGRPFVAVLGAMLELGSQAAMEHEALGRRLAGLNPSAIVW
KGLHAEDVRKGLTLAGYAGPWQVVTDAGEFMKTWQTLQDYGLENKAAKSGGVVLFKGSRSNRLETLVAVLSS
>Mature_472_residues
MRITINEAAVCLGLPGTLPDAENTVLTSVVTDSRDAGPGALFVCVPGSRVDGHDFAAAAVRQGAAAVLAAGPLPGINVPV
LVVPDTVKALGQLAGLWRDRTKARVVAVTGTAGKTTLKEVLAQVLSLGGKTARNAMNHNNQIGMPRAVLGTDGDEKFWIM
EAGISQQGDMDDLGSVLRPDIAVVLNAGTGHTEGLGEKGVAWHKARLLKYLAKGGKGLVCADYPDLVREARATETDICFF
SGSGRDAEFRAVYAGAAPQGAAVTPSDAYGLYRLWLGGREFDVTAPFRGAYGAENVAAVAAVAHMLGLDLQAIRQGLAQA
RMPAQRFNQVQAGSWLLIDDTYNANPLSMGRMLDAAAERAAGRPFVAVLGAMLELGSQAAMEHEALGRRLAGLNPSAIVW
KGLHAEDVRKGLTLAGYAGPWQVVTDAGEFMKTWQTLQDYGLENKAAKSGGVVLFKGSRSNRLETLVAVLSS

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=462, Percent_Identity=33.3333333333333, Blast_Score=171, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49465; Mature: 49465

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITINEAAVCLGLPGTLPDAENTVLTSVVTDSRDAGPGALFVCVPGSRVDGHDFAAAAV
CEEEECCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHHH
RQGAAAVLAAGPLPGINVPVLVVPDTVKALGQLAGLWRDRTKARVVAVTGTAGKTTLKEV
HCCCEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHH
LAQVLSLGGKTARNAMNHNNQIGMPRAVLGTDGDEKFWIMEAGISQQGDMDDLGSVLRPD
HHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHCCC
IAVVLNAGTGHTEGLGEKGVAWHKARLLKYLAKGGKGLVCADYPDLVREARATETDICFF
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCEEEEE
SGSGRDAEFRAVYAGAAPQGAAVTPSDAYGLYRLWLGGREFDVTAPFRGAYGAENVAAVA
ECCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCCCCHHHHHH
AVAHMLGLDLQAIRQGLAQARMPAQRFNQVQAGSWLLIDDTYNANPLSMGRMLDAAAERA
HHHHHHCCCHHHHHHHHHHHCCCHHHHCHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
AGRPFVAVLGAMLELGSQAAMEHEALGRRLAGLNPSAIVWKGLHAEDVRKGLTLAGYAGP
CCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCCEEEECCCC
WQVVTDAGEFMKTWQTLQDYGLENKAAKSGGVVLFKGSRSNRLETLVAVLSS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure
MRITINEAAVCLGLPGTLPDAENTVLTSVVTDSRDAGPGALFVCVPGSRVDGHDFAAAAV
CEEEECCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHHH
RQGAAAVLAAGPLPGINVPVLVVPDTVKALGQLAGLWRDRTKARVVAVTGTAGKTTLKEV
HCCCEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHH
LAQVLSLGGKTARNAMNHNNQIGMPRAVLGTDGDEKFWIMEAGISQQGDMDDLGSVLRPD
HHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHCCC
IAVVLNAGTGHTEGLGEKGVAWHKARLLKYLAKGGKGLVCADYPDLVREARATETDICFF
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCEEEEE
SGSGRDAEFRAVYAGAAPQGAAVTPSDAYGLYRLWLGGREFDVTAPFRGAYGAENVAAVA
ECCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCCCCHHHHHH
AVAHMLGLDLQAIRQGLAQARMPAQRFNQVQAGSWLLIDDTYNANPLSMGRMLDAAAERA
HHHHHHCCCHHHHHHHHHHHCCCHHHHCHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
AGRPFVAVLGAMLELGSQAAMEHEALGRRLAGLNPSAIVWKGLHAEDVRKGLTLAGYAGP
CCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCCEEEECCCC
WQVVTDAGEFMKTWQTLQDYGLENKAAKSGGVVLFKGSRSNRLETLVAVLSS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]