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The map label for this gene is ispG [H]

Identifier: 220904223

GI number: 220904223

Start: 1106304

End: 1107374

Strand: Direct

Name: ispG [H]

Synonym: Ddes_0950

Alternate gene names: 220904223

Gene position: 1106304-1107374 (Clockwise)

Preceding gene: 220904218

Following gene: 220904224

Centisome position: 38.5

GC content: 62.37

Gene sequence:

>1071_bases
ATGGGCAGGAATACCAGAGCAATACGCCTTGGCGGCGTTACTGTGGGCGGCGGCGCGCCTGTTGTTGTTCAGAGCATGAC
CAATACGGATACCCGCGATCCGCAGGCCACCCTGGGGCAGATTGCCCGTCTGGCGGCCCGTGGCTGTGAAATGGTGCGCC
TGGCCGTACCTGATGAGGCGGCCGTGGCGGCCCTGCCCGCCATACGATCAGGTACGCCCCTGCCGCTTATCGCCGACATA
CACTTTGACTACCGCCTTGCCGTGGGCGCGCTGGAGGCGGGCCTTGAGGGGTTGCGCATCAATCCCGGCAATATTGGCCC
CAAGGCGCATGTGGACAGGGTGGTAGATGCGGCCAAGGCGCACGGCGCGGTTATTCGCGTGGGCGTCAATTCCGGCTCGG
TGGAAAAGGATATCCTGCGCAAGTATGGCGGGCCTTGCCCCGAAGCTCTGGTGGAAAGCGCCCTTGGGCATGTGCGTCTG
CTGGAAGCCCGCGCTTTTTACGACACCAAGATTTCCCTCAAGTCTTCCTCTGTGCTGGATACCATAACAGCCTACCGTCT
GCTGGCCGCTGCCTGTGATTATCCTACACATATCGGCATCACCGAGGCCGGGGGCCTCATGCGGGGAACGGTCAAATCTG
CCGTAGGGCTGGGCATCCTGCTGCACGAGGGCATAGGCGACACCCTGCGCGTGTCGCTGACGGCAGATCCCGCCGAAGAA
GTGACCGTGGCCTGGGAGATACTGCGGGCCCTGGGCCTCCGCTCCCGCGGGCCGGAGATTATTTCCTGCCCCACCTGCGG
CCGCACCGAGATAGATCTTTTTGCCCTGGCACGCGCGGTAGAAGACCGCCTGGCAACATCCACGGCCTCCATAAAGGTGG
CGGTTATGGGCTGCGTGGTCAATGGTCCCGGGGAGGCGCGCGAGGCTGATCTTGGCGTGGCCGGGGGCCGCGACAAAGGC
ATTATTTTCCGCAAGGGCGAGGTCATCCGCTCCGTGAAAGGGCAGGAAGCCTTGCTGGCGGCTTTTATGGAAGAACTGCA
ATCACTGCTTAACGAAAAGGAAAATACCTAA

Upstream 100 bases:

>100_bases
GATCGGCTGTCTGCTTGGCTTGCTGCATGAAATAGCTTACAAGACGCCTTTAAAGGATATGGCGACGCAGCGCCGCCAAG
TTTTTCATCTGGGAGAAGGC

Downstream 100 bases:

>100_bases
TGCGCTTCAGCTCCTGCTATATTCCCACGCTCAAGGAATCTCCGGCCGATGCGGAAGTGATCAGCCACAAGCTGCTGCTG
CGGGCGGGGATGGTGCGGCG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MGRNTRAIRLGGVTVGGGAPVVVQSMTNTDTRDPQATLGQIARLAARGCEMVRLAVPDEAAVAALPAIRSGTPLPLIADI
HFDYRLAVGALEAGLEGLRINPGNIGPKAHVDRVVDAAKAHGAVIRVGVNSGSVEKDILRKYGGPCPEALVESALGHVRL
LEARAFYDTKISLKSSSVLDTITAYRLLAAACDYPTHIGITEAGGLMRGTVKSAVGLGILLHEGIGDTLRVSLTADPAEE
VTVAWEILRALGLRSRGPEIISCPTCGRTEIDLFALARAVEDRLATSTASIKVAVMGCVVNGPGEAREADLGVAGGRDKG
IIFRKGEVIRSVKGQEALLAAFMEELQSLLNEKENT

Sequences:

>Translated_356_residues
MGRNTRAIRLGGVTVGGGAPVVVQSMTNTDTRDPQATLGQIARLAARGCEMVRLAVPDEAAVAALPAIRSGTPLPLIADI
HFDYRLAVGALEAGLEGLRINPGNIGPKAHVDRVVDAAKAHGAVIRVGVNSGSVEKDILRKYGGPCPEALVESALGHVRL
LEARAFYDTKISLKSSSVLDTITAYRLLAAACDYPTHIGITEAGGLMRGTVKSAVGLGILLHEGIGDTLRVSLTADPAEE
VTVAWEILRALGLRSRGPEIISCPTCGRTEIDLFALARAVEDRLATSTASIKVAVMGCVVNGPGEAREADLGVAGGRDKG
IIFRKGEVIRSVKGQEALLAAFMEELQSLLNEKENT
>Mature_355_residues
GRNTRAIRLGGVTVGGGAPVVVQSMTNTDTRDPQATLGQIARLAARGCEMVRLAVPDEAAVAALPAIRSGTPLPLIADIH
FDYRLAVGALEAGLEGLRINPGNIGPKAHVDRVVDAAKAHGAVIRVGVNSGSVEKDILRKYGGPCPEALVESALGHVRLL
EARAFYDTKISLKSSSVLDTITAYRLLAAACDYPTHIGITEAGGLMRGTVKSAVGLGILLHEGIGDTLRVSLTADPAEEV
TVAWEILRALGLRSRGPEIISCPTCGRTEIDLFALARAVEDRLATSTASIKVAVMGCVVNGPGEAREADLGVAGGRDKGI
IFRKGEVIRSVKGQEALLAAFMEELQSLLNEKENT

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=324, Percent_Identity=50.6172839506173, Blast_Score=326, Evalue=1e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 37263; Mature: 37132

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRNTRAIRLGGVTVGGGAPVVVQSMTNTDTRDPQATLGQIARLAARGCEMVRLAVPDEA
CCCCCCEEEECCEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHH
AVAALPAIRSGTPLPLIADIHFDYRLAVGALEAGLEGLRINPGNIGPKAHVDRVVDAAKA
HHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHC
HGAVIRVGVNSGSVEKDILRKYGGPCPEALVESALGHVRLLEARAFYDTKISLKSSSVLD
CCCEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHCCEEEEEHHHHCCEEEEECCCHHHH
TITAYRLLAAACDYPTHIGITEAGGLMRGTVKSAVGLGILLHEGIGDTLRVSLTADPAEE
HHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHEEEECCCCCEEEEEEECCCHHH
VTVAWEILRALGLRSRGPEIISCPTCGRTEIDLFALARAVEDRLATSTASIKVAVMGCVV
HHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
NGPGEAREADLGVAGGRDKGIIFRKGEVIRSVKGQEALLAAFMEELQSLLNEKENT
CCCCCCCCCCCCCCCCCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GRNTRAIRLGGVTVGGGAPVVVQSMTNTDTRDPQATLGQIARLAARGCEMVRLAVPDEA
CCCCCEEEECCEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHH
AVAALPAIRSGTPLPLIADIHFDYRLAVGALEAGLEGLRINPGNIGPKAHVDRVVDAAKA
HHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHC
HGAVIRVGVNSGSVEKDILRKYGGPCPEALVESALGHVRLLEARAFYDTKISLKSSSVLD
CCCEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHCCEEEEEHHHHCCEEEEECCCHHHH
TITAYRLLAAACDYPTHIGITEAGGLMRGTVKSAVGLGILLHEGIGDTLRVSLTADPAEE
HHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHEEEECCCCCEEEEEEECCCHHH
VTVAWEILRALGLRSRGPEIISCPTCGRTEIDLFALARAVEDRLATSTASIKVAVMGCVV
HHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
NGPGEAREADLGVAGGRDKGIIFRKGEVIRSVKGQEALLAAFMEELQSLLNEKENT
CCCCCCCCCCCCCCCCCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA