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The map label for this gene is proS

Identifier: 220904224

GI number: 220904224

Start: 1107374

End: 1109098

Strand: Direct

Name: proS

Synonym: Ddes_0951

Alternate gene names: 220904224

Gene position: 1107374-1109098 (Clockwise)

Preceding gene: 220904223

Following gene: 220904225

Centisome position: 38.54

GC content: 60.35

Gene sequence:

>1725_bases
ATGCGCTTCAGCTCCTGCTATATTCCCACGCTCAAGGAATCTCCGGCCGATGCGGAAGTGATCAGCCACAAGCTGCTGCT
GCGGGCGGGGATGGTGCGGCGGCTTACATCCGGCCTGTATATTTATCTGCCCCTGGGCCTGCGGGTGATCAACAAGATCG
CCCGCACCGTGCGCGAAGAAATGGAAAAAGCCGGTTTTCGCGAACTGCTCATGCCTATGGTGCAGCCTGGCGACCTGTGG
AAAGAAACAGGCCGCTGGGAGCACTACGGCAAGGAGCTGCTGCGCTTCAAGGACCGGAACGAGCGTGAATACTGCCTCGG
CCCCACCCACGAGGAGGTCATCACCGACCTCGTGCGCGGCGAGGTGCGTTCTTACCGCCAGTTGCCTGTGCGCCTGTATC
AGGTGCAGACCAAGTTTCGCGATGAAATACGCCCCCGTTTCGGGCTTATGCGCGGGCGAGAATTCATGATGAAAGACGGC
TATTCTTTTGATGCCACTGCCGAAGGGGCCGAAGAAAGCTATAAGATCATGTACGATGCCTACATGAGTATTTTCAGCCG
TCTTGGTCTGCGGTTTCGCGCGGTAGAAGCCGACACCGGCTCCATTGGCGGCAATTTTTCCCATGAATTCATGGTGCTGG
CCGACACAGGCGAAGATACCATCGCCTTCTGTCACGACTGTGAGTACGCCGCCAACGTGGAGCGGGCCGAGGTTGCCTGG
CGGGGCAGCCCCTCGACAGGGGGGTGCCCCGCCATGGAAAAAATCGCCACGCCCGGCGCGCATAGCGTGGAAGAGCTGAC
GGCCCTGCTGGGAGTTCCCGCCAGTGCCATCGTCAAGACCATGCTTTTCAAGGTGGACGGCAAAACTGTGGCCGTGCTTG
TGCGCGGCGACCGCGAGGTCAACGACATCAAGCTGAAAAATCTGCTCAAGGCGCAGGAAGTGGAGCTGGCCGATGCTGCA
ACAGTGCAGGCCGTTACAGCCGCCCCTGTGGGCTTTGCCGGGCCTGTGGCGCTTGATGTGCCTGTTTATGCCGATGCCGA
ACTTCAAGGCGGCACGGACTATGTGGTAGGAGCCAATGCGGCTGACGCCCACCTGAAGCACGTGGATCTTGCCCGCGACG
CCGCGGTAACCGCCTGGGCGGACCTGCGCGCCATCACCGCTGACGACCAGTGTCCCCGCTGCGGCGGCCGCATGGAACTG
ACCAGGGGCATTGAGGTGGGGCATATTTTCATGCTCGGCCTCAAGTACAGTGAAGCCATGCACGCTGTTTTTCTGGATGA
AAACGGCAAGGAGCGGACCATGATTATGGGCTGCTACGGCATAGGCGTTTCCCGCGTGGCCGCTGCTGCCATTGAGCAGA
ACCACGATGAGCACGGCATTGTTTTCCCGCCGCCGGTAGCTCCTTTCGAATGTGTGCTGCTTAACCTTGATCCCCGCAGT
GAAGAGGTCAACGCCAAGGTTGAAGAAATCTACGCCCTGCTGCAGGGCATGGGGATCGAAGTGCTGCTGGACGACCGCGA
AGAGCGGCCCGGCGTCAAGTTCAAGGATGCCGACCTGCTGGGTATCCCCATGCAGCTTGTGGTGGGCGGCAAGGGCCTTG
GGCGTGGTATTGTGGAATGCAAAGACCGCCGTACAGGCGAAAAGGGCGAGCTTTCCGCCGCCAGTCTGGAGCAGGATTTT
GCCGCCTGGTCTGAAAAAGTGCGGCAGGGCTGGGCCAGCCGATAG

Upstream 100 bases:

>100_bases
GCAAGGGCGAGGTCATCCGCTCCGTGAAAGGGCAGGAAGCCTTGCTGGCGGCTTTTATGGAAGAACTGCAATCACTGCTT
AACGAAAAGGAAAATACCTA

Downstream 100 bases:

>100_bases
AGCCTTTTTTGAAGTAACCGCCGCCCGCAGAGATGTAATTTTTGCGGGCGGCGTCTCATTGGTGCACTGTGCCGGTTGCC
GTTCGGGCCATTGTGTCCGG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MRFSSCYIPTLKESPADAEVISHKLLLRAGMVRRLTSGLYIYLPLGLRVINKIARTVREEMEKAGFRELLMPMVQPGDLW
KETGRWEHYGKELLRFKDRNEREYCLGPTHEEVITDLVRGEVRSYRQLPVRLYQVQTKFRDEIRPRFGLMRGREFMMKDG
YSFDATAEGAEESYKIMYDAYMSIFSRLGLRFRAVEADTGSIGGNFSHEFMVLADTGEDTIAFCHDCEYAANVERAEVAW
RGSPSTGGCPAMEKIATPGAHSVEELTALLGVPASAIVKTMLFKVDGKTVAVLVRGDREVNDIKLKNLLKAQEVELADAA
TVQAVTAAPVGFAGPVALDVPVYADAELQGGTDYVVGANAADAHLKHVDLARDAAVTAWADLRAITADDQCPRCGGRMEL
TRGIEVGHIFMLGLKYSEAMHAVFLDENGKERTMIMGCYGIGVSRVAAAAIEQNHDEHGIVFPPPVAPFECVLLNLDPRS
EEVNAKVEEIYALLQGMGIEVLLDDREERPGVKFKDADLLGIPMQLVVGGKGLGRGIVECKDRRTGEKGELSAASLEQDF
AAWSEKVRQGWASR

Sequences:

>Translated_574_residues
MRFSSCYIPTLKESPADAEVISHKLLLRAGMVRRLTSGLYIYLPLGLRVINKIARTVREEMEKAGFRELLMPMVQPGDLW
KETGRWEHYGKELLRFKDRNEREYCLGPTHEEVITDLVRGEVRSYRQLPVRLYQVQTKFRDEIRPRFGLMRGREFMMKDG
YSFDATAEGAEESYKIMYDAYMSIFSRLGLRFRAVEADTGSIGGNFSHEFMVLADTGEDTIAFCHDCEYAANVERAEVAW
RGSPSTGGCPAMEKIATPGAHSVEELTALLGVPASAIVKTMLFKVDGKTVAVLVRGDREVNDIKLKNLLKAQEVELADAA
TVQAVTAAPVGFAGPVALDVPVYADAELQGGTDYVVGANAADAHLKHVDLARDAAVTAWADLRAITADDQCPRCGGRMEL
TRGIEVGHIFMLGLKYSEAMHAVFLDENGKERTMIMGCYGIGVSRVAAAAIEQNHDEHGIVFPPPVAPFECVLLNLDPRS
EEVNAKVEEIYALLQGMGIEVLLDDREERPGVKFKDADLLGIPMQLVVGGKGLGRGIVECKDRRTGEKGELSAASLEQDF
AAWSEKVRQGWASR
>Mature_574_residues
MRFSSCYIPTLKESPADAEVISHKLLLRAGMVRRLTSGLYIYLPLGLRVINKIARTVREEMEKAGFRELLMPMVQPGDLW
KETGRWEHYGKELLRFKDRNEREYCLGPTHEEVITDLVRGEVRSYRQLPVRLYQVQTKFRDEIRPRFGLMRGREFMMKDG
YSFDATAEGAEESYKIMYDAYMSIFSRLGLRFRAVEADTGSIGGNFSHEFMVLADTGEDTIAFCHDCEYAANVERAEVAW
RGSPSTGGCPAMEKIATPGAHSVEELTALLGVPASAIVKTMLFKVDGKTVAVLVRGDREVNDIKLKNLLKAQEVELADAA
TVQAVTAAPVGFAGPVALDVPVYADAELQGGTDYVVGANAADAHLKHVDLARDAAVTAWADLRAITADDQCPRCGGRMEL
TRGIEVGHIFMLGLKYSEAMHAVFLDENGKERTMIMGCYGIGVSRVAAAAIEQNHDEHGIVFPPPVAPFECVLLNLDPRS
EEVNAKVEEIYALLQGMGIEVLLDDREERPGVKFKDADLLGIPMQLVVGGKGLGRGIVECKDRRTGEKGELSAASLEQDF
AAWSEKVRQGWASR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=280, Percent_Identity=43.2142857142857, Blast_Score=248, Evalue=8e-66,
Organism=Homo sapiens, GI38570111, Length=160, Percent_Identity=28.75, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI38202255, Length=164, Percent_Identity=28.6585365853659, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1786392, Length=554, Percent_Identity=51.985559566787, Blast_Score=576, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI115532348, Length=260, Percent_Identity=36.5384615384615, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI193203271, Length=93, Percent_Identity=37.6344086021505, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=558, Percent_Identity=30.4659498207885, Blast_Score=253, Evalue=7e-68,
Organism=Saccharomyces cerevisiae, GI6322654, Length=162, Percent_Identity=28.3950617283951, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322112, Length=164, Percent_Identity=25.609756097561, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24656200, Length=274, Percent_Identity=36.8613138686131, Blast_Score=185, Evalue=9e-47,
Organism=Drosophila melanogaster, GI24583839, Length=162, Percent_Identity=25.9259259259259, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24583842, Length=162, Percent_Identity=25.9259259259259, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28574141, Length=162, Percent_Identity=25.9259259259259, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_DESDA (B8IZD1)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479536.1
- GeneID:   7284631
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_0951
- HOGENOM:   HBG403504
- ProtClustDB:   PRK09194
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63429; Mature: 63429

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSSCYIPTLKESPADAEVISHKLLLRAGMVRRLTSGLYIYLPLGLRVINKIARTVREE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHH
MEKAGFRELLMPMVQPGDLWKETGRWEHYGKELLRFKDRNEREYCLGPTHEEVITDLVRG
HHHCCHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
EVRSYRQLPVRLYQVQTKFRDEIRPRFGLMRGREFMMKDGYSFDATAEGAEESYKIMYDA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHH
YMSIFSRLGLRFRAVEADTGSIGGNFSHEFMVLADTGEDTIAFCHDCEYAANVERAEVAW
HHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEEECCHHHCCCCHHEEEE
RGSPSTGGCPAMEKIATPGAHSVEELTALLGVPASAIVKTMLFKVDGKTVAVLVRGDREV
CCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHEECCCEEEEEEECCCCC
NDIKLKNLLKAQEVELADAATVQAVTAAPVGFAGPVALDVPVYADAELQGGTDYVVGANA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEECCCCCCCCCEEEECCC
ADAHLKHVDLARDAAVTAWADLRAITADDQCPRCGGRMELTRGIEVGHIFMLGLKYSEAM
CHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCEEECCCCCEEEEEEEEEEHHCCE
HAVFLDENGKERTMIMGCYGIGVSRVAAAAIEQNHDEHGIVFPPPVAPFECVLLNLDPRS
EEEEECCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCEECCCCCCCEEEEEECCCCC
EEVNAKVEEIYALLQGMGIEVLLDDREERPGVKFKDADLLGIPMQLVVGGKGLGRGIVEC
HHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCEECCCCEECCCEEEEECCCCCCCCHHHH
KDRRTGEKGELSAASLEQDFAAWSEKVRQGWASR
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRFSSCYIPTLKESPADAEVISHKLLLRAGMVRRLTSGLYIYLPLGLRVINKIARTVREE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHH
MEKAGFRELLMPMVQPGDLWKETGRWEHYGKELLRFKDRNEREYCLGPTHEEVITDLVRG
HHHCCHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
EVRSYRQLPVRLYQVQTKFRDEIRPRFGLMRGREFMMKDGYSFDATAEGAEESYKIMYDA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHH
YMSIFSRLGLRFRAVEADTGSIGGNFSHEFMVLADTGEDTIAFCHDCEYAANVERAEVAW
HHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEEECCHHHCCCCHHEEEE
RGSPSTGGCPAMEKIATPGAHSVEELTALLGVPASAIVKTMLFKVDGKTVAVLVRGDREV
CCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHEECCCEEEEEEECCCCC
NDIKLKNLLKAQEVELADAATVQAVTAAPVGFAGPVALDVPVYADAELQGGTDYVVGANA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEECCCCCCCCCEEEECCC
ADAHLKHVDLARDAAVTAWADLRAITADDQCPRCGGRMELTRGIEVGHIFMLGLKYSEAM
CHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCEEECCCCCEEEEEEEEEEHHCCE
HAVFLDENGKERTMIMGCYGIGVSRVAAAAIEQNHDEHGIVFPPPVAPFECVLLNLDPRS
EEEEECCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCEECCCCCCCEEEEEECCCCC
EEVNAKVEEIYALLQGMGIEVLLDDREERPGVKFKDADLLGIPMQLVVGGKGLGRGIVEC
HHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCEECCCCEECCCEEEEECCCCCCCCHHHH
KDRRTGEKGELSAASLEQDFAAWSEKVRQGWASR
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA