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The map label for this gene is paaK [H]

Identifier: 220904212

GI number: 220904212

Start: 1093579

End: 1094877

Strand: Direct

Name: paaK [H]

Synonym: Ddes_0939

Alternate gene names: 220904212

Gene position: 1093579-1094877 (Clockwise)

Preceding gene: 220904211

Following gene: 220904213

Centisome position: 38.06

GC content: 58.04

Gene sequence:

>1299_bases
ATGGAAGTTTTTGATCCAGCGGAACTGTGGAGCCGGGAGCGCATAGAGGAAACCCAGCTTGTCCGGCTGAAAAATATGGT
CGCTCAGGCCCGTAAGTGTGATTTTTACCGGCAGCGCCTCGACGAGGCGGGAATCGGGCCGGACTCTTTGCACAGCCTTG
ATGACCTGCGCCGCATCCCCTTCACCACCAAAGACGATCTGCGTACCCAGTATCCCACCGGCATGCTTTGCGTGCCTCAG
GCTGAAATTGTGCGTATGCACTGCTCAAGCGGCACTACCGGGTCGCCCGTAGCCATCTGTCACACGCAGAACGATATCAA
TTCCTGGGCCGACCTTATGGCGCGCTGCATGCACATGGTGGGCGTGCGCCGTGAGGACGTATTTCAGAATATGTCCGGTT
ACGGTCTGTTCACGGGCGGTTTGGGCATCCATTTCGGGGCCGAACGCCTGGGCTGCCTGACCATCCCCGCCGGTGCGGGA
AACTCGCGCCGCCAGATCAAGCTGGCCAAGGACTTTCGCACCACTGTGGCGCATATTCTGCCTTCCTATGCGCTCATTCT
TGGCGAACACCTGCGCAATATGGGTGAAGACCCGCGTGAATTCCCCTTGCGCATCGCTCTTGTGGGCGCGGAGCCGTATA
CTGAAGAATTCCGCCGTCGTATTGAAAGCCTTTTTGATATGAAGGCGTATAACTCCTACGGGCTTTCCGAAATGAACGGG
CCGGGCGTGGCCTTTGAATGCCTTGAGCAGGCCGGCATGCACCTGTGGGAAGACGCCTATATTCCTGAAATTGTCGATCC
GGAAACAGGCCAGCCTGTACCCGAGGGCCAGGTGGGCGAGTTGGTCATGACCTGTCTCTGCCGCCAGGGGATGCCCATTC
TGCGCTATCGTACCCGCGACCTTACCCGTTTTCTGCCCGGAGTATGCGGCTGTGGGCGCATGCACCGCCGCATGGACCGT
ATCCTCGGCCGCGCGGACGACATGTTTATCATCAAGGGCGTCAATGTTTACCCCATGCAGGTCGAGCAGGTCATCATGAC
CTTCCCTGAAGTGGGGCAGAGCTATCTTATCCTGCTGGAGAACGACGGTATCGGCGATGTCATGCGGGTGCAGGTGGAAG
TGCGCGACGAATTCTTTGTGGAAGACATGCGCGTTCTGCAAAGCCTGCAGAAAACTATTGCCCAGCGCCTGCGTGATGAA
ATCCTCATCACGCCCAGGGTAGAGCTTGTGCAGAGCAACAGCCTGCCGCGCGCCGAAGGCAAGGCCGTTCGCCTGCAAGA
CATGCGCGAAAAAAAATAA

Upstream 100 bases:

>100_bases
ACGCATGCCGATTGATCTGCAGGCGCAACCTGCGGCCTGATCCGGTTCGGAGGCTGACCTCCGGAAGTTTTGACAATGCT
CCAGTAAGGTGAGGAGAGGC

Downstream 100 bases:

>100_bases
TACCGGAAACTGTATGGATTTTTTGCCATTTCCCCTTGCCAGTCTGCTGGCGGGCGCATTCATCACCTTGCTGGGCTTCG
TGCTTTTGCTCAACGTCTTC

Product: Phenylacetate--CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MEVFDPAELWSRERIEETQLVRLKNMVAQARKCDFYRQRLDEAGIGPDSLHSLDDLRRIPFTTKDDLRTQYPTGMLCVPQ
AEIVRMHCSSGTTGSPVAICHTQNDINSWADLMARCMHMVGVRREDVFQNMSGYGLFTGGLGIHFGAERLGCLTIPAGAG
NSRRQIKLAKDFRTTVAHILPSYALILGEHLRNMGEDPREFPLRIALVGAEPYTEEFRRRIESLFDMKAYNSYGLSEMNG
PGVAFECLEQAGMHLWEDAYIPEIVDPETGQPVPEGQVGELVMTCLCRQGMPILRYRTRDLTRFLPGVCGCGRMHRRMDR
ILGRADDMFIIKGVNVYPMQVEQVIMTFPEVGQSYLILLENDGIGDVMRVQVEVRDEFFVEDMRVLQSLQKTIAQRLRDE
ILITPRVELVQSNSLPRAEGKAVRLQDMREKK

Sequences:

>Translated_432_residues
MEVFDPAELWSRERIEETQLVRLKNMVAQARKCDFYRQRLDEAGIGPDSLHSLDDLRRIPFTTKDDLRTQYPTGMLCVPQ
AEIVRMHCSSGTTGSPVAICHTQNDINSWADLMARCMHMVGVRREDVFQNMSGYGLFTGGLGIHFGAERLGCLTIPAGAG
NSRRQIKLAKDFRTTVAHILPSYALILGEHLRNMGEDPREFPLRIALVGAEPYTEEFRRRIESLFDMKAYNSYGLSEMNG
PGVAFECLEQAGMHLWEDAYIPEIVDPETGQPVPEGQVGELVMTCLCRQGMPILRYRTRDLTRFLPGVCGCGRMHRRMDR
ILGRADDMFIIKGVNVYPMQVEQVIMTFPEVGQSYLILLENDGIGDVMRVQVEVRDEFFVEDMRVLQSLQKTIAQRLRDE
ILITPRVELVQSNSLPRAEGKAVRLQDMREKK
>Mature_432_residues
MEVFDPAELWSRERIEETQLVRLKNMVAQARKCDFYRQRLDEAGIGPDSLHSLDDLRRIPFTTKDDLRTQYPTGMLCVPQ
AEIVRMHCSSGTTGSPVAICHTQNDINSWADLMARCMHMVGVRREDVFQNMSGYGLFTGGLGIHFGAERLGCLTIPAGAG
NSRRQIKLAKDFRTTVAHILPSYALILGEHLRNMGEDPREFPLRIALVGAEPYTEEFRRRIESLFDMKAYNSYGLSEMNG
PGVAFECLEQAGMHLWEDAYIPEIVDPETGQPVPEGQVGELVMTCLCRQGMPILRYRTRDLTRFLPGVCGCGRMHRRMDR
ILGRADDMFIIKGVNVYPMQVEQVIMTFPEVGQSYLILLENDGIGDVMRVQVEVRDEFFVEDMRVLQSLQKTIAQRLRDE
ILITPRVELVQSNSLPRAEGKAVRLQDMREKK

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=429, Percent_Identity=42.8904428904429, Blast_Score=356, Evalue=2e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 49164; Mature: 49164

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVFDPAELWSRERIEETQLVRLKNMVAQARKCDFYRQRLDEAGIGPDSLHSLDDLRRIP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
FTTKDDLRTQYPTGMLCVPQAEIVRMHCSSGTTGSPVAICHTQNDINSWADLMARCMHMV
CCCCHHHHHCCCCCEEECCHHHHHEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHH
GVRREDVFQNMSGYGLFTGGLGIHFGAERLGCLTIPAGAGNSRRQIKLAKDFRTTVAHIL
CCCHHHHHHCCCCCEEEECCCEEEECCCCCCEEEECCCCCCCCCEEEHHHHHHHHHHHHH
PSYALILGEHLRNMGEDPREFPLRIALVGAEPYTEEFRRRIESLFDMKAYNSYGLSEMNG
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PGVAFECLEQAGMHLWEDAYIPEIVDPETGQPVPEGQVGELVMTCLCRQGMPILRYRTRD
CCHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
LTRFLPGVCGCGRMHRRMDRILGRADDMFIIKGVNVYPMQVEQVIMTFPEVGQSYLILLE
HHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEE
NDGIGDVMRVQVEVRDEFFVEDMRVLQSLQKTIAQRLRDEILITPRVELVQSNSLPRAEG
CCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHCCCCCCCCC
KAVRLQDMREKK
CEEEHHHHHCCC
>Mature Secondary Structure
MEVFDPAELWSRERIEETQLVRLKNMVAQARKCDFYRQRLDEAGIGPDSLHSLDDLRRIP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
FTTKDDLRTQYPTGMLCVPQAEIVRMHCSSGTTGSPVAICHTQNDINSWADLMARCMHMV
CCCCHHHHHCCCCCEEECCHHHHHEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHH
GVRREDVFQNMSGYGLFTGGLGIHFGAERLGCLTIPAGAGNSRRQIKLAKDFRTTVAHIL
CCCHHHHHHCCCCCEEEECCCEEEECCCCCCEEEECCCCCCCCCEEEHHHHHHHHHHHHH
PSYALILGEHLRNMGEDPREFPLRIALVGAEPYTEEFRRRIESLFDMKAYNSYGLSEMNG
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PGVAFECLEQAGMHLWEDAYIPEIVDPETGQPVPEGQVGELVMTCLCRQGMPILRYRTRD
CCHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
LTRFLPGVCGCGRMHRRMDRILGRADDMFIIKGVNVYPMQVEQVIMTFPEVGQSYLILLE
HHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEE
NDGIGDVMRVQVEVRDEFFVEDMRVLQSLQKTIAQRLRDEILITPRVELVQSNSLPRAEG
CCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHCCCCCCCCC
KAVRLQDMREKK
CEEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]