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The map label for this gene is exoA [H]

Identifier: 220904154

GI number: 220904154

Start: 1030491

End: 1031297

Strand: Direct

Name: exoA [H]

Synonym: Ddes_0881

Alternate gene names: 220904154

Gene position: 1030491-1031297 (Clockwise)

Preceding gene: 220904151

Following gene: 220904155

Centisome position: 35.86

GC content: 56.13

Gene sequence:

>807_bases
ATGCTGTTTAAACTCGTATCCTGGAATGTCAACGGGCTGAGGGCGGTTTCTGCCAAGGCTGATTGGGACTGGTTTGCGCA
CAGTGATGCCCAGGTGGTGGCCTTGCAGGAAACCAAGGCGCATCCGGAACAACTGGACGAGGACGTGCGTGAGCCTGCGG
GCTGGGAAAGCCGCTGGGCGTCAAGCACTGTCAAAAAAGGGTATTCCGGCGTGGCCGTGTTCAGCCGTGTACCTTTTCTT
TCGTGGAGTGCCGAACTGCCGCAGCCGGAATTTCAGGGTGAGGGCCGTCTGCTGCATCTGGAATATCCGGATTTTCATTT
TTTCAACGGCTACTTCCCCAACGGCGGAGCCGAAGAACTGGATGAAAACGGCAAACCCACAGGCAACTTCAAGCGGGTTC
CCTACAAGATGGCTTACTTCGAGGCTTTTCTGGCCTATGCCGAAGAGTGCCGCAAGAGCAAACCCATAGTGGTCTGCGGC
GATTTTAACATAGCACACAGGCCCATTGATCTGGCCCGGCCCAAGCCCAATGAAAAGTGCACGGGGTTTCTGCCTGAAGA
GCGCGCTTTTCTCGACCGCTTCACGGCCCTGGGCTATGTGGATACTTTCCGCCATGTGCATGGCGATGCGCCGGATAACT
ATTCGTGGTGGTCATACAAGACCCGCGCACGTGCCAAGAATGTGGGGTGGCGCATTGACTATTTCTTCGTGTCGCAAGAG
CTTGCGCCCGCCATCCGCGATGCCTGGATAGAAAATGACGTGTACGGTTCAGACCACTGCCCTGTGGGCCTGAGCCTGGA
AATATGA

Upstream 100 bases:

>100_bases
CAGTAAAATGCGTGTTTGCGCAATCTTCGCCGGTGGGCGTCATTGACGGCAGCTCTTGCGAGGCGGGGCGTGCAAGGCTA
TGCTCGGTACCGGAGGTTTT

Downstream 100 bases:

>100_bases
AAAAGCGCTGCCATGAGTGAATCATGGCAGCCACTTTTTTCGGGCGGACGACATCACGCTTGCCCGGCCGTTGCCACGCC
TGTCGCACCCGATTTTCTGG

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MLFKLVSWNVNGLRAVSAKADWDWFAHSDAQVVALQETKAHPEQLDEDVREPAGWESRWASSTVKKGYSGVAVFSRVPFL
SWSAELPQPEFQGEGRLLHLEYPDFHFFNGYFPNGGAEELDENGKPTGNFKRVPYKMAYFEAFLAYAEECRKSKPIVVCG
DFNIAHRPIDLARPKPNEKCTGFLPEERAFLDRFTALGYVDTFRHVHGDAPDNYSWWSYKTRARAKNVGWRIDYFFVSQE
LAPAIRDAWIENDVYGSDHCPVGLSLEI

Sequences:

>Translated_268_residues
MLFKLVSWNVNGLRAVSAKADWDWFAHSDAQVVALQETKAHPEQLDEDVREPAGWESRWASSTVKKGYSGVAVFSRVPFL
SWSAELPQPEFQGEGRLLHLEYPDFHFFNGYFPNGGAEELDENGKPTGNFKRVPYKMAYFEAFLAYAEECRKSKPIVVCG
DFNIAHRPIDLARPKPNEKCTGFLPEERAFLDRFTALGYVDTFRHVHGDAPDNYSWWSYKTRARAKNVGWRIDYFFVSQE
LAPAIRDAWIENDVYGSDHCPVGLSLEI
>Mature_268_residues
MLFKLVSWNVNGLRAVSAKADWDWFAHSDAQVVALQETKAHPEQLDEDVREPAGWESRWASSTVKKGYSGVAVFSRVPFL
SWSAELPQPEFQGEGRLLHLEYPDFHFFNGYFPNGGAEELDENGKPTGNFKRVPYKMAYFEAFLAYAEECRKSKPIVVCG
DFNIAHRPIDLARPKPNEKCTGFLPEERAFLDRFTALGYVDTFRHVHGDAPDNYSWWSYKTRARAKNVGWRIDYFFVSQE
LAPAIRDAWIENDVYGSDHCPVGLSLEI

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=268, Percent_Identity=38.0597014925373, Blast_Score=192, Evalue=2e-49,
Organism=Homo sapiens, GI18375503, Length=268, Percent_Identity=38.0597014925373, Blast_Score=192, Evalue=2e-49,
Organism=Homo sapiens, GI18375501, Length=268, Percent_Identity=38.0597014925373, Blast_Score=192, Evalue=2e-49,
Organism=Escherichia coli, GI1788046, Length=276, Percent_Identity=29.7101449275362, Blast_Score=121, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI71989536, Length=268, Percent_Identity=33.955223880597, Blast_Score=153, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI71989539, Length=118, Percent_Identity=33.8983050847458, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221330655, Length=262, Percent_Identity=36.6412213740458, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI17136678, Length=262, Percent_Identity=36.6412213740458, Blast_Score=182, Evalue=2e-46,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30682; Mature: 30682

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00727 AP_NUCLEASE_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFKLVSWNVNGLRAVSAKADWDWFAHSDAQVVALQETKAHPEQLDEDVREPAGWESRWA
CEEEEEECCCCCEEEEECCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHH
SSTVKKGYSGVAVFSRVPFLSWSAELPQPEFQGEGRLLHLEYPDFHFFNGYFPNGGAEEL
HHHHHCCCCCEEHHHHCCCEECCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCHHHH
DENGKPTGNFKRVPYKMAYFEAFLAYAEECRKSKPIVVCGDFNIAHRPIDLARPKPNEKC
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCC
TGFLPEERAFLDRFTALGYVDTFRHVHGDAPDNYSWWSYKTRARAKNVGWRIDYFFVSQE
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCEEEEEEEECHH
LAPAIRDAWIENDVYGSDHCPVGLSLEI
HHHHHHHHHHCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MLFKLVSWNVNGLRAVSAKADWDWFAHSDAQVVALQETKAHPEQLDEDVREPAGWESRWA
CEEEEEECCCCCEEEEECCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHH
SSTVKKGYSGVAVFSRVPFLSWSAELPQPEFQGEGRLLHLEYPDFHFFNGYFPNGGAEEL
HHHHHCCCCCEEHHHHCCCEECCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCHHHH
DENGKPTGNFKRVPYKMAYFEAFLAYAEECRKSKPIVVCGDFNIAHRPIDLARPKPNEKC
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCC
TGFLPEERAFLDRFTALGYVDTFRHVHGDAPDNYSWWSYKTRARAKNVGWRIDYFFVSQE
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCEEEEEEEECHH
LAPAIRDAWIENDVYGSDHCPVGLSLEI
HHHHHHHHHHCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]