Click here to switch to the map view.

The map label for this gene is ntrX [H]

Identifier: 220904091

GI number: 220904091

Start: 955901

End: 957256

Strand: Direct

Name: ntrX [H]

Synonym: Ddes_0817

Alternate gene names: 220904091

Gene position: 955901-957256 (Clockwise)

Preceding gene: 220904090

Following gene: 220904095

Centisome position: 33.27

GC content: 63.79

Gene sequence:

>1356_bases
ATGCGCGTGCTTGTTGTGGATGACGAATCAGCCTTTGCCGGGCCTTTGGCCCAGCGCCTGAGCCTGCGCGGCATGGATGC
GCGCACCGCCCGCGATGCGCGTGAGGCTTTGTCCATCCTGCAGGCATGGCCCGCGGAACTGGTGTTTCTTGATGTGGGCC
TGCCCGGTATGGACGGCGTTGCCCTGCTCAAGCTTTTGCGCGAGCAATATGCACAGACGGATGTGGTCATGCTTTCGGGT
TCCGCAGATATGGGCAAAGCTGTGCAGGCCATGCGGCGGGGAGCGCTGAACTGGCTCTCCAAGCCCGCGGATATTGAGCA
GGTGCTTGAAGAATGCCGCAAGGCGCGGGAGCGGGCCGCAGCCCGGCAAGAGGCGGCCCGCCTTGCCGAAGCCGCGCGCT
GGCGCAGTCTGGGGCGGGTTGCCGAAGGGGTGGCCCATGAGGTGAATAATCCCCTTAACATCATGATGCAGGCCGCCGGG
CTTATCCGCGATTGCCTGGATGAACCGGAGGCTCAGGCCCTGGCTGATGTGGACGAAATGCGCGAAGCCGTCAATACCAT
TCGCGCCCAGAGTCTGCGCGTGCGGGAAATAACGCGTAAGCTGCTCATGGTGGGGCATGGGCTGGATGCGCGCACCGGGC
CGCTGGATGTGGCTGCGGTTATTGATGAAAGCCTTGATCTGCTGCGCCACAGGATTGAAGCGGCAGGTCTGCGCTGCCAG
GTGGATCTTTCCGGCGCGTATGTGTTCCCGGGCCATGAAGCTGCTGCACAGGAAACTGCTGCACAGGAAACTGCTGCGCC
GGGCGCTCTCGGGGAAGCTGCTGCGGAGGCGGCGGAAGAGGCGGGCAGCGCTGCTCCGCGCCCTTGGGGTTCGGCCCCGG
AATTGCGGCAGATATTTCTGCATCTATTTGAAAATGCTCTGGATGCCATGCCTCATGGTGGATTGGTGCAGGTCTCGGCC
AGGCTGAGACGTGACGGACAGGGAAGGAGCTGGTACGATCTGCTCGTTGCCGACAGCGGCCCCGGCATTGAACCGGACAT
CCTGCCCCATATTTTTGAACCATTTTTCAGTTCGCGTGCCCTTCAAGGCGGATTCGGCGGTCATGACCAATACCATGACA
GCCATACCAGCGGCAGTGCCATGCGCCCCGGAACTGCCGTACAATCCCACATGGGCCGCTATGCGGGTCTCGGCCTTGCC
GTTGCCCGCTCGCTGGCGCACGCGCGTGGCGGCGAACTCTCCGCCGCCAACAGCCAGCAAGGCGGGGCAGTTTTCTGCCT
GAGCCTTCCCCTGGCACAAGCGCCCGCGGCCTCCGGTCAGGCTGCGGGTGACCGCTCTCCGGACGGCGGCCTTTGA

Upstream 100 bases:

>100_bases
CTTTGAATCCGCGCGCGATGTGATGAAAGAAAAGGATATCCTGCAGGAACACGAAAAATAAACAGCGCCCAGCGGGTTGT
ACTGCAGAAAGGGGAACGCC

Downstream 100 bases:

>100_bases
GGCCGCCCCCGGATGGCGCTGTAAATCCTGCCTGCACACCACAACTGCCGGCCACGCAAAACCGGCGTATCCGGTATCAT
TCCAGAGCATTTCAACATCG

Product: response regulator receiver sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MRVLVVDDESAFAGPLAQRLSLRGMDARTARDAREALSILQAWPAELVFLDVGLPGMDGVALLKLLREQYAQTDVVMLSG
SADMGKAVQAMRRGALNWLSKPADIEQVLEECRKARERAAARQEAARLAEAARWRSLGRVAEGVAHEVNNPLNIMMQAAG
LIRDCLDEPEAQALADVDEMREAVNTIRAQSLRVREITRKLLMVGHGLDARTGPLDVAAVIDESLDLLRHRIEAAGLRCQ
VDLSGAYVFPGHEAAAQETAAQETAAPGALGEAAAEAAEEAGSAAPRPWGSAPELRQIFLHLFENALDAMPHGGLVQVSA
RLRRDGQGRSWYDLLVADSGPGIEPDILPHIFEPFFSSRALQGGFGGHDQYHDSHTSGSAMRPGTAVQSHMGRYAGLGLA
VARSLAHARGGELSAANSQQGGAVFCLSLPLAQAPAASGQAAGDRSPDGGL

Sequences:

>Translated_451_residues
MRVLVVDDESAFAGPLAQRLSLRGMDARTARDAREALSILQAWPAELVFLDVGLPGMDGVALLKLLREQYAQTDVVMLSG
SADMGKAVQAMRRGALNWLSKPADIEQVLEECRKARERAAARQEAARLAEAARWRSLGRVAEGVAHEVNNPLNIMMQAAG
LIRDCLDEPEAQALADVDEMREAVNTIRAQSLRVREITRKLLMVGHGLDARTGPLDVAAVIDESLDLLRHRIEAAGLRCQ
VDLSGAYVFPGHEAAAQETAAQETAAPGALGEAAAEAAEEAGSAAPRPWGSAPELRQIFLHLFENALDAMPHGGLVQVSA
RLRRDGQGRSWYDLLVADSGPGIEPDILPHIFEPFFSSRALQGGFGGHDQYHDSHTSGSAMRPGTAVQSHMGRYAGLGLA
VARSLAHARGGELSAANSQQGGAVFCLSLPLAQAPAASGQAAGDRSPDGGL
>Mature_451_residues
MRVLVVDDESAFAGPLAQRLSLRGMDARTARDAREALSILQAWPAELVFLDVGLPGMDGVALLKLLREQYAQTDVVMLSG
SADMGKAVQAMRRGALNWLSKPADIEQVLEECRKARERAAARQEAARLAEAARWRSLGRVAEGVAHEVNNPLNIMMQAAG
LIRDCLDEPEAQALADVDEMREAVNTIRAQSLRVREITRKLLMVGHGLDARTGPLDVAAVIDESLDLLRHRIEAAGLRCQ
VDLSGAYVFPGHEAAAQETAAQETAAPGALGEAAAEAAEEAGSAAPRPWGSAPELRQIFLHLFENALDAMPHGGLVQVSA
RLRRDGQGRSWYDLLVADSGPGIEPDILPHIFEPFFSSRALQGGFGGHDQYHDSHTSGSAMRPGTAVQSHMGRYAGLGLA
VARSLAHARGGELSAANSQQGGAVFCLSLPLAQAPAASGQAAGDRSPDGGL

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=130, Percent_Identity=29.2307692307692, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790299, Length=122, Percent_Identity=30.327868852459, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081816, Length=229, Percent_Identity=30.5676855895196, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 47863; Mature: 47863

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00063 ALDOKETO_REDUCTASE_3 ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLVVDDESAFAGPLAQRLSLRGMDARTARDAREALSILQAWPAELVFLDVGLPGMDGV
CEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHH
ALLKLLREQYAQTDVVMLSGSADMGKAVQAMRRGALNWLSKPADIEQVLEECRKARERAA
HHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ARQEAARLAEAARWRSLGRVAEGVAHEVNNPLNIMMQAAGLIRDCLDEPEAQALADVDEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
REAVNTIRAQSLRVREITRKLLMVGHGLDARTGPLDVAAVIDESLDLLRHRIEAAGLRCQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
VDLSGAYVFPGHEAAAQETAAQETAAPGALGEAAAEAAEEAGSAAPRPWGSAPELRQIFL
EEECCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
HLFENALDAMPHGGLVQVSARLRRDGQGRSWYDLLVADSGPGIEPDILPHIFEPFFSSRA
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHC
LQGGFGGHDQYHDSHTSGSAMRPGTAVQSHMGRYAGLGLAVARSLAHARGGELSAANSQQ
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GGAVFCLSLPLAQAPAASGQAAGDRSPDGGL
CCEEEEEECCHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRVLVVDDESAFAGPLAQRLSLRGMDARTARDAREALSILQAWPAELVFLDVGLPGMDGV
CEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHH
ALLKLLREQYAQTDVVMLSGSADMGKAVQAMRRGALNWLSKPADIEQVLEECRKARERAA
HHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ARQEAARLAEAARWRSLGRVAEGVAHEVNNPLNIMMQAAGLIRDCLDEPEAQALADVDEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
REAVNTIRAQSLRVREITRKLLMVGHGLDARTGPLDVAAVIDESLDLLRHRIEAAGLRCQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
VDLSGAYVFPGHEAAAQETAAQETAAPGALGEAAAEAAEEAGSAAPRPWGSAPELRQIFL
EEECCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
HLFENALDAMPHGGLVQVSARLRRDGQGRSWYDLLVADSGPGIEPDILPHIFEPFFSSRA
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHC
LQGGFGGHDQYHDSHTSGSAMRPGTAVQSHMGRYAGLGLAVARSLAHARGGELSAANSQQ
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GGAVFCLSLPLAQAPAASGQAAGDRSPDGGL
CCEEEEEECCHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]