Click here to switch to the map view.

The map label for this gene is mprA [H]

Identifier: 220904090

GI number: 220904090

Start: 955388

End: 955861

Strand: Direct

Name: mprA [H]

Synonym: Ddes_0816

Alternate gene names: 220904090

Gene position: 955388-955861 (Clockwise)

Preceding gene: 220904089

Following gene: 220904091

Centisome position: 33.25

GC content: 57.17

Gene sequence:

>474_bases
ATGACCAAGGAAGACATCAAGATTCTGCTGGTGGACGATGAAAGGCAGTTTGTGGACACGCTGGCCGAACGTCTGGCCAT
GCGTGGTTTTGCCGCCCGCGTGGCTTATGACGGCCCCGAGGCGCTCAAAGCCGTGGATCAGCCCACGGATGTCATCGTGC
TTGACCTGCGCATGCCGGGCATGGACGGCTTTGAGGTGCTGCGCAATGTAAAGAAGAGCAACCCTCAGGTGCAGGTCATC
ATCCTGACCGGCCACGGGGGGGATGCCGAGGAACAGACCGCCTACCGCATGGGCGCGTATAATTTTCTCCGCAAGCCTAT
GGATATTGATGAGCTGCTTGGCAGCATCCGCATGGCCTATCGCGACAAGCTGGAAAACGCCATGGTGGCGGTGTCGTTGG
CTGAAGGCGGCGACTTTGAATCCGCGCGCGATGTGATGAAAGAAAAGGATATCCTGCAGGAACACGAAAAATAA

Upstream 100 bases:

>100_bases
GCGCGAAGCGGAACCGCGTTTTTTCCTTTCACTTTCCGCCTGCGATTCAGATGCGGTATAACCGGCTCCGGGTATAGTCC
GGACCTCCAAGGAGTTTTTC

Downstream 100 bases:

>100_bases
ACAGCGCCCAGCGGGTTGTACTGCAGAAAGGGGAACGCCATGCGCGTGCTTGTTGTGGATGACGAATCAGCCTTTGCCGG
GCCTTTGGCCCAGCGCCTGA

Product: response regulator receiver protein

Products: NADP; 2 glutathione [C]

Alternate protein names: Mycobacterial persistence regulator A [H]

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MTKEDIKILLVDDERQFVDTLAERLAMRGFAARVAYDGPEALKAVDQPTDVIVLDLRMPGMDGFEVLRNVKKSNPQVQVI
ILTGHGGDAEEQTAYRMGAYNFLRKPMDIDELLGSIRMAYRDKLENAMVAVSLAEGGDFESARDVMKEKDILQEHEK

Sequences:

>Translated_157_residues
MTKEDIKILLVDDERQFVDTLAERLAMRGFAARVAYDGPEALKAVDQPTDVIVLDLRMPGMDGFEVLRNVKKSNPQVQVI
ILTGHGGDAEEQTAYRMGAYNFLRKPMDIDELLGSIRMAYRDKLENAMVAVSLAEGGDFESARDVMKEKDILQEHEK
>Mature_156_residues
TKEDIKILLVDDERQFVDTLAERLAMRGFAARVAYDGPEALKAVDQPTDVIVLDLRMPGMDGFEVLRNVKKSNPQVQVII
LTGHGGDAEEQTAYRMGAYNFLRKPMDIDELLGSIRMAYRDKLENAMVAVSLAEGGDFESARDVMKEKDILQEHEK

Specific function: Member of the two-component regulatory system mprB/mprA which contributes to maintaining a balance among several systems involved in stress resistance and is required for establishment and maintenance of persistent infection in the host. Functions as a tr

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=113, Percent_Identity=37.1681415929204, Blast_Score=79, Evalue=1e-16,
Organism=Escherichia coli, GI1790437, Length=140, Percent_Identity=31.4285714285714, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1788550, Length=107, Percent_Identity=35.5140186915888, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1788905, Length=117, Percent_Identity=35.042735042735, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI2367329, Length=115, Percent_Identity=38.2608695652174, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1790860, Length=115, Percent_Identity=32.1739130434783, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1789402, Length=108, Percent_Identity=32.4074074074074, Blast_Score=60, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 17660; Mature: 17529

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEDIKILLVDDERQFVDTLAERLAMRGFAARVAYDGPEALKAVDQPTDVIVLDLRMPG
CCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCC
MDGFEVLRNVKKSNPQVQVIILTGHGGDAEEQTAYRMGAYNFLRKPMDIDELLGSIRMAY
CCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RDKLENAMVAVSLAEGGDFESARDVMKEKDILQEHEK
HHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKEDIKILLVDDERQFVDTLAERLAMRGFAARVAYDGPEALKAVDQPTDVIVLDLRMPG
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCC
MDGFEVLRNVKKSNPQVQVIILTGHGGDAEEQTAYRMGAYNFLRKPMDIDELLGSIRMAY
CCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RDKLENAMVAVSLAEGGDFESARDVMKEKDILQEHEK
HHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA