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The map label for this gene is wcaJ [H]

Identifier: 220903393

GI number: 220903393

Start: 139534

End: 140883

Strand: Reverse

Name: wcaJ [H]

Synonym: Ddes_0107

Alternate gene names: 220903393

Gene position: 140883-139534 (Counterclockwise)

Preceding gene: 220903394

Following gene: 220903388

Centisome position: 4.9

GC content: 57.56

Gene sequence:

>1350_bases
ATGATAAGCAGCTATCGCATGGGTCTTTTGCAGGTTCTGGACCTGTTCTGCATCCTGCTGGCCCTGACTATTTCAGGCAT
GACGACCATTGCCCCGGACCTGAGCGTTTTTGACGACTACACCGGGGCTTCGCTCTTTACCATTTTTTTCTATATGCTGT
TTTTCTATATCCTTGATGCTTACAATGTAGGTAACGAGGACTTCAAGGAGACCACAGGCCGCGTACTTGTGGCCTGCCTG
CTGGCCATCGTGTCCTCTGCCACGGCATCCTACGCCTTTCAGCACTGGCGCTTTGACCGCAAGACCATCACCCTGCTTTT
CGCGCTTTCTTTCTGCCTCAGCCTGCTGTGGCGCTGGCTTTACCACCTCAATGCCGACAGGCTCACCCACCCCCTGCGCA
TCCTGCTGGTGGGCGTGGACCGCGCAGGCAAGGTGCGGCAACTGCTGGCCGAAGGCCTGCCCCAGGCGGAAATTCTCGGC
TATGTGGGCGAGCGTGATCAGGGGCCGGATGCCGGACCCTGCCTTGGCCCGCCCTTTCTGGCTCTGGACATAGCCCAGGA
AAAAAAGGCCACCATGATACTGTTGCTGCCCGATGCGCCCATTGATGACGACATCGCCCACGAGCTTTTGCAGGCCAAGC
TGCGCGGCAGCATGGTGGTGGACATACGCAGTTTTTATGAACATGTGGTGCAGCGCCTGCCGCTCTCACAGCTCACCGAT
GAGTGGCTGCTACAGACCGAAGGTTTTTCGCTGAATACGCGCGGTTCACTGCGTCGCCTCAAACGGGCCCTGGATGTGCT
GATCTCGCTGCTGCTGCTTATACCCGCCACGCCCGTGATGCTGCTCACGGCCCTTATCGTGCGACTGGAGTCGCCCGGCC
CGGTCATTTACAAGCAGGACCGCGTGGGCCTTTTTGAAAAGGAATTCACGGTGTACAAGTTCCGCTCCATGCGGGCCGAT
GCAGAAAAAGACGGCGCCGTCTGGGCCAGCGCCAAAGACAGCCGGGTCACTTTTTTCGGCCGCTTTATCCGTAAGGTACG
CATAGATGAGCTGCCGCAGATATGGAACATCCTCAAGGGCGACATGAGCTTTATCGGGCCGCGCCCCGAGCGCATGACCT
TTGTGCAGAAACTCAAGGAAACCATCCCGTACTACAGCCTGCGACACACGGTAAAACCCGGCCTGACCGGCTGGGCGCAG
GTGTGCTACCCCTACGGCGCGTCTGAAGAAGACGCACGCCGCAAGCTGGAGTACGATCTCTATTACATCAAAAATATGTC
CATCCTGCTGGACATAAACATTATCTTCAAAACTATCGGCGTGGTGCTCTTCCCCAAGGGCGCGCGGTAA

Upstream 100 bases:

>100_bases
GGTGGCATCTGTCCTGCACCCGTAAGAACAGCGGCGACAAGTACGGCTCAAGCATTACACGCTATTGGCAAGGCTAATCT
CCTTCAGAGGATAATTTGCA

Downstream 100 bases:

>100_bases
CGCCAGGGCAGCCCTAGCGGTCCGGGCGTAGCCCCCGGCCATTCCCCTTGCAGCCAGCAATGCAAAACCCCGCCACACGG
ATAATTGGGATCCGTGTGGC

Product: exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MISSYRMGLLQVLDLFCILLALTISGMTTIAPDLSVFDDYTGASLFTIFFYMLFFYILDAYNVGNEDFKETTGRVLVACL
LAIVSSATASYAFQHWRFDRKTITLLFALSFCLSLLWRWLYHLNADRLTHPLRILLVGVDRAGKVRQLLAEGLPQAEILG
YVGERDQGPDAGPCLGPPFLALDIAQEKKATMILLLPDAPIDDDIAHELLQAKLRGSMVVDIRSFYEHVVQRLPLSQLTD
EWLLQTEGFSLNTRGSLRRLKRALDVLISLLLLIPATPVMLLTALIVRLESPGPVIYKQDRVGLFEKEFTVYKFRSMRAD
AEKDGAVWASAKDSRVTFFGRFIRKVRIDELPQIWNILKGDMSFIGPRPERMTFVQKLKETIPYYSLRHTVKPGLTGWAQ
VCYPYGASEEDARRKLEYDLYYIKNMSILLDINIIFKTIGVVLFPKGAR

Sequences:

>Translated_449_residues
MISSYRMGLLQVLDLFCILLALTISGMTTIAPDLSVFDDYTGASLFTIFFYMLFFYILDAYNVGNEDFKETTGRVLVACL
LAIVSSATASYAFQHWRFDRKTITLLFALSFCLSLLWRWLYHLNADRLTHPLRILLVGVDRAGKVRQLLAEGLPQAEILG
YVGERDQGPDAGPCLGPPFLALDIAQEKKATMILLLPDAPIDDDIAHELLQAKLRGSMVVDIRSFYEHVVQRLPLSQLTD
EWLLQTEGFSLNTRGSLRRLKRALDVLISLLLLIPATPVMLLTALIVRLESPGPVIYKQDRVGLFEKEFTVYKFRSMRAD
AEKDGAVWASAKDSRVTFFGRFIRKVRIDELPQIWNILKGDMSFIGPRPERMTFVQKLKETIPYYSLRHTVKPGLTGWAQ
VCYPYGASEEDARRKLEYDLYYIKNMSILLDINIIFKTIGVVLFPKGAR
>Mature_449_residues
MISSYRMGLLQVLDLFCILLALTISGMTTIAPDLSVFDDYTGASLFTIFFYMLFFYILDAYNVGNEDFKETTGRVLVACL
LAIVSSATASYAFQHWRFDRKTITLLFALSFCLSLLWRWLYHLNADRLTHPLRILLVGVDRAGKVRQLLAEGLPQAEILG
YVGERDQGPDAGPCLGPPFLALDIAQEKKATMILLLPDAPIDDDIAHELLQAKLRGSMVVDIRSFYEHVVQRLPLSQLTD
EWLLQTEGFSLNTRGSLRRLKRALDVLISLLLLIPATPVMLLTALIVRLESPGPVIYKQDRVGLFEKEFTVYKFRSMRAD
AEKDGAVWASAKDSRVTFFGRFIRKVRIDELPQIWNILKGDMSFIGPRPERMTFVQKLKETIPYYSLRHTVKPGLTGWAQ
VCYPYGASEEDARRKLEYDLYYIKNMSILLDINIIFKTIGVVLFPKGAR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=187, Percent_Identity=42.7807486631016, Blast_Score=161, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 51072; Mature: 51072

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISSYRMGLLQVLDLFCILLALTISGMTTIAPDLSVFDDYTGASLFTIFFYMLFFYILDA
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHH
YNVGNEDFKETTGRVLVACLLAIVSSATASYAFQHWRFDRKTITLLFALSFCLSLLWRWL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
YHLNADRLTHPLRILLVGVDRAGKVRQLLAEGLPQAEILGYVGERDQGPDAGPCLGPPFL
HHCCHHHHHCCEEEEEEECCCCHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCE
ALDIAQEKKATMILLLPDAPIDDDIAHELLQAKLRGSMVVDIRSFYEHVVQRLPLSQLTD
EEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCHHHCCH
EWLLQTEGFSLNTRGSLRRLKRALDVLISLLLLIPATPVMLLTALIVRLESPGPVIYKQD
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECC
RVGLFEKEFTVYKFRSMRADAEKDGAVWASAKDSRVTFFGRFIRKVRIDELPQIWNILKG
CCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
DMSFIGPRPERMTFVQKLKETIPYYSLRHTVKPGLTGWAQVCYPYGASEEDARRKLEYDL
CHHHCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHE
YYIKNMSILLDINIIFKTIGVVLFPKGAR
EEEECCEEEEEHHHHHHHHHHEECCCCCC
>Mature Secondary Structure
MISSYRMGLLQVLDLFCILLALTISGMTTIAPDLSVFDDYTGASLFTIFFYMLFFYILDA
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHH
YNVGNEDFKETTGRVLVACLLAIVSSATASYAFQHWRFDRKTITLLFALSFCLSLLWRWL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
YHLNADRLTHPLRILLVGVDRAGKVRQLLAEGLPQAEILGYVGERDQGPDAGPCLGPPFL
HHCCHHHHHCCEEEEEEECCCCHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCE
ALDIAQEKKATMILLLPDAPIDDDIAHELLQAKLRGSMVVDIRSFYEHVVQRLPLSQLTD
EEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCHHHCCH
EWLLQTEGFSLNTRGSLRRLKRALDVLISLLLLIPATPVMLLTALIVRLESPGPVIYKQD
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECC
RVGLFEKEFTVYKFRSMRADAEKDGAVWASAKDSRVTFFGRFIRKVRIDELPQIWNILKG
CCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
DMSFIGPRPERMTFVQKLKETIPYYSLRHTVKPGLTGWAQVCYPYGASEEDARRKLEYDL
CHHHCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHE
YYIKNMSILLDINIIFKTIGVVLFPKGAR
EEEECCEEEEEHHHHHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]