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The map label for this gene is cimA [H]

Identifier: 220903388

GI number: 220903388

Start: 131915

End: 133750

Strand: Reverse

Name: cimA [H]

Synonym: Ddes_0102

Alternate gene names: 220903388

Gene position: 133750-131915 (Counterclockwise)

Preceding gene: 220903393

Following gene: 220903387

Centisome position: 4.65

GC content: 57.35

Gene sequence:

>1836_bases
ATGAGCAATATCATCCACCAAAGCGGAGGCAGCCGTACCCTGGTCATGCAGGAGCCTGAAACCCCCCAGCTCTACCGTGA
ACTTTTCCCTTATACCAGTATCTGCCGCACTTCTTTTGATGAAGTGCTGCTGGCGCCGCGCCCTGCGGAACAGATGCGCA
TTACCGACACCACCTTTCGTGACGGCCAGCAGGCACGCCCGCCGTATACGGTCAAACAGGTGGCAAAAATGTTTGACTTT
CTCCACCGTCTCGGCGGCAAGACGGGGCTTATCACGGCTTCGGAATTTTTTCTGTATTCCGCCAAGGACCGCAAGTGCAT
TGACGTATGCCGCGCGCGCGGCTACCGCTTCCCGCGCGTGACTGCCTGGATACGTGCCACCAGGGATGACCTCAAGCTGG
CCCGCGATATGGAGTTTGACGAAACCGGCATGCTCACCAGCGTGTCGGACTATCACATTTTTCTCAAACTGGGCAAAACC
CGCCAGCAGGCCATGGACATGTACGTGGGCATGGCCGAACAGGCGCTGGAATGGGGCATCATACCCCGCTGCCACTTTGA
AGACATCACCAGGGCCGATATTTACGGCTTCTGCCTGCCCCTGGCCCAGAGGCTCATGGAGCTTTCACGCCAGAGCGGCA
TGCCCGTAAAAATACGCCTGTGCGACACGATGGGCTACGGCGTACCCTACCCGGGCGCAGCCCTGCCCCGCTCTGTGCAG
CGCATTGTGCGTGCCTTTACCGACGAGGCGGGCGTTCCCGGCCAGTGGCTTGAATGGCACGGGCATAACGACTTTCACAA
AGTGCTCGTCAACGGCGTCACGGCATGGCTCAACGGCTGCGGCGCTGTCAACAGCACACTGTTCGGCTTTGGCGAACGCA
CGGGCAACACCCCGCTTGAGGCCCTGCTTGTGGAGTATATCTCCCTTACCGGCGACGATGCCGCCGCCGACACCACCGTG
CTGCACGAAGTGGCACAGTTTTTTGAAAAAGAGCTGGATTACCGCATTCCCCACAACTATCCCTTTGTGGGGCGCGACTT
CAACGCCACCAGTGCGGGCGTGCATGCTGACGGCCTGGCAAAAAACGAAGAGATCTACAATATTTTCGACACCAAGCATC
TGCTCGGCCGCTCCGTGCCCATCATCATTACCGACAAGACGGGCCGCGCGGGCGTGGCGTACTGGATCAACACCAATCTC
AATCTGGAAAAAGAGCGGCAGATATCCAAAAAGCATCCTGCTGTGGGCAAAATCTACGATGCCATCATGGCTGTTTACGA
AGAAACCGGGCGCACTACCAATTTCTCGCATGTGGAAATGGAAGCCCTGGTACAGCGCTTCATGCCCGAACTTTTTGTGT
CGGAGTTCGACAACATGAAACAGCTCGCGGGCGAACTGGCCGCCAACCTGCTTGTGCGCCTTGCCCGCGACAAGGACCTT
CTGGACTTCGGCGAAAAGGCCCACGCCAAGATCGACGCCTTTGAACGCGAATATCCCTTTATCCAGTACTGTTACCTTAC
GGACGCCACAGGCAGCCTCAAATGCTCTGCCATCACCGACCCCATGTACAAGGAAACCTACGAGGCCCTGCCCATCGGCT
ATGATTTCTCCGGCCGTGAATGGTTCAAAAAACCCATGCAAAGCGGCGACCTGCACATTATGGATGTGTACCAGTCGCAC
TTTACGAGCAAACTCATCATTACGGTCTCCTGCGCCGTCACAGATGAAAAAGACAATATTGCCGGGGTGATCGGTGTGGA
CATTCAGCTTGAGGAACTGCTCAAGCGCGCCCGCTCGCTCAAGCACGAGGTCGGCACCGTGGATGACGGGGACTAG

Upstream 100 bases:

>100_bases
AGGCCCCGCACCGTACTGGACGAGACCCCCGTAAGCCGCTATAACAGGCCTTCTCCGGAAAACTTTTTTCCGCGCGGTCA
GCGCGAGTCGGCGGGAGCAT

Downstream 100 bases:

>100_bases
ACCGCTTTTCAGCATCAGACCGGGGCCGGGCTTGGGATGGCCCGGCCCTGCTTCATATTCAACGCTATTACAAAAGGACG
GGGGAACATGCGCAAGACTG

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 611; Mature: 610

Protein sequence:

>611_residues
MSNIIHQSGGSRTLVMQEPETPQLYRELFPYTSICRTSFDEVLLAPRPAEQMRITDTTFRDGQQARPPYTVKQVAKMFDF
LHRLGGKTGLITASEFFLYSAKDRKCIDVCRARGYRFPRVTAWIRATRDDLKLARDMEFDETGMLTSVSDYHIFLKLGKT
RQQAMDMYVGMAEQALEWGIIPRCHFEDITRADIYGFCLPLAQRLMELSRQSGMPVKIRLCDTMGYGVPYPGAALPRSVQ
RIVRAFTDEAGVPGQWLEWHGHNDFHKVLVNGVTAWLNGCGAVNSTLFGFGERTGNTPLEALLVEYISLTGDDAAADTTV
LHEVAQFFEKELDYRIPHNYPFVGRDFNATSAGVHADGLAKNEEIYNIFDTKHLLGRSVPIIITDKTGRAGVAYWINTNL
NLEKERQISKKHPAVGKIYDAIMAVYEETGRTTNFSHVEMEALVQRFMPELFVSEFDNMKQLAGELAANLLVRLARDKDL
LDFGEKAHAKIDAFEREYPFIQYCYLTDATGSLKCSAITDPMYKETYEALPIGYDFSGREWFKKPMQSGDLHIMDVYQSH
FTSKLIITVSCAVTDEKDNIAGVIGVDIQLEELLKRARSLKHEVGTVDDGD

Sequences:

>Translated_611_residues
MSNIIHQSGGSRTLVMQEPETPQLYRELFPYTSICRTSFDEVLLAPRPAEQMRITDTTFRDGQQARPPYTVKQVAKMFDF
LHRLGGKTGLITASEFFLYSAKDRKCIDVCRARGYRFPRVTAWIRATRDDLKLARDMEFDETGMLTSVSDYHIFLKLGKT
RQQAMDMYVGMAEQALEWGIIPRCHFEDITRADIYGFCLPLAQRLMELSRQSGMPVKIRLCDTMGYGVPYPGAALPRSVQ
RIVRAFTDEAGVPGQWLEWHGHNDFHKVLVNGVTAWLNGCGAVNSTLFGFGERTGNTPLEALLVEYISLTGDDAAADTTV
LHEVAQFFEKELDYRIPHNYPFVGRDFNATSAGVHADGLAKNEEIYNIFDTKHLLGRSVPIIITDKTGRAGVAYWINTNL
NLEKERQISKKHPAVGKIYDAIMAVYEETGRTTNFSHVEMEALVQRFMPELFVSEFDNMKQLAGELAANLLVRLARDKDL
LDFGEKAHAKIDAFEREYPFIQYCYLTDATGSLKCSAITDPMYKETYEALPIGYDFSGREWFKKPMQSGDLHIMDVYQSH
FTSKLIITVSCAVTDEKDNIAGVIGVDIQLEELLKRARSLKHEVGTVDDGD
>Mature_610_residues
SNIIHQSGGSRTLVMQEPETPQLYRELFPYTSICRTSFDEVLLAPRPAEQMRITDTTFRDGQQARPPYTVKQVAKMFDFL
HRLGGKTGLITASEFFLYSAKDRKCIDVCRARGYRFPRVTAWIRATRDDLKLARDMEFDETGMLTSVSDYHIFLKLGKTR
QQAMDMYVGMAEQALEWGIIPRCHFEDITRADIYGFCLPLAQRLMELSRQSGMPVKIRLCDTMGYGVPYPGAALPRSVQR
IVRAFTDEAGVPGQWLEWHGHNDFHKVLVNGVTAWLNGCGAVNSTLFGFGERTGNTPLEALLVEYISLTGDDAAADTTVL
HEVAQFFEKELDYRIPHNYPFVGRDFNATSAGVHADGLAKNEEIYNIFDTKHLLGRSVPIIITDKTGRAGVAYWINTNLN
LEKERQISKKHPAVGKIYDAIMAVYEETGRTTNFSHVEMEALVQRFMPELFVSEFDNMKQLAGELAANLLVRLARDKDLL
DFGEKAHAKIDAFEREYPFIQYCYLTDATGSLKCSAITDPMYKETYEALPIGYDFSGREWFKKPMQSGDLHIMDVYQSHF
TSKLIITVSCAVTDEKDNIAGVIGVDIQLEELLKRARSLKHEVGTVDDGD

Specific function: Catalyzes the condensation of pyruvate and acetyl- coenzyme A to form (R)-citramalate [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=458, Percent_Identity=24.0174672489083, Blast_Score=102, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6320071, Length=388, Percent_Identity=23.4536082474227, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320019, Length=408, Percent_Identity=23.7745098039216, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.1.182 [H]

Molecular weight: Translated: 69136; Mature: 69004

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIIHQSGGSRTLVMQEPETPQLYRELFPYTSICRTSFDEVLLAPRPAEQMRITDTTFR
CCCCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHHHCCHHHHEECCCCHHHEEEECCCCC
DGQQARPPYTVKQVAKMFDFLHRLGGKTGLITASEFFLYSAKDRKCIDVCRARGYRFPRV
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHEEEEECCCCHHHHHHHHCCCCCCHH
TAWIRATRDDLKLARDMEFDETGMLTSVSDYHIFLKLGKTRQQAMDMYVGMAEQALEWGI
HHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCC
IPRCHFEDITRADIYGFCLPLAQRLMELSRQSGMPVKIRLCDTMGYGVPYPGAALPRSVQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHH
RIVRAFTDEAGVPGQWLEWHGHNDFHKVLVNGVTAWLNGCGAVNSTLFGFGERTGNTPLE
HHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHH
ALLVEYISLTGDDAAADTTVLHEVAQFFEKELDYRIPHNYPFVGRDFNATSAGVHADGLA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KNEEIYNIFDTKHLLGRSVPIIITDKTGRAGVAYWINTNLNLEKERQISKKHPAVGKIYD
CCCHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHH
AIMAVYEETGRTTNFSHVEMEALVQRFMPELFVSEFDNMKQLAGELAANLLVRLARDKDL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LDFGEKAHAKIDAFEREYPFIQYCYLTDATGSLKCSAITDPMYKETYEALPIGYDFSGRE
HHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCHHHHHHHHHCCCCCCCCCHH
WFKKPMQSGDLHIMDVYQSHFTSKLIITVSCAVTDEKDNIAGVIGVDIQLEELLKRARSL
HHHHHHCCCCEEEEHHHHHHCCCEEEEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHH
KHEVGTVDDGD
HHHCCCCCCCC
>Mature Secondary Structure 
SNIIHQSGGSRTLVMQEPETPQLYRELFPYTSICRTSFDEVLLAPRPAEQMRITDTTFR
CCCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHHHCCHHHHEECCCCHHHEEEECCCCC
DGQQARPPYTVKQVAKMFDFLHRLGGKTGLITASEFFLYSAKDRKCIDVCRARGYRFPRV
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHEEEEECCCCHHHHHHHHCCCCCCHH
TAWIRATRDDLKLARDMEFDETGMLTSVSDYHIFLKLGKTRQQAMDMYVGMAEQALEWGI
HHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCC
IPRCHFEDITRADIYGFCLPLAQRLMELSRQSGMPVKIRLCDTMGYGVPYPGAALPRSVQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHH
RIVRAFTDEAGVPGQWLEWHGHNDFHKVLVNGVTAWLNGCGAVNSTLFGFGERTGNTPLE
HHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHH
ALLVEYISLTGDDAAADTTVLHEVAQFFEKELDYRIPHNYPFVGRDFNATSAGVHADGLA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KNEEIYNIFDTKHLLGRSVPIIITDKTGRAGVAYWINTNLNLEKERQISKKHPAVGKIYD
CCCHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHH
AIMAVYEETGRTTNFSHVEMEALVQRFMPELFVSEFDNMKQLAGELAANLLVRLARDKDL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LDFGEKAHAKIDAFEREYPFIQYCYLTDATGSLKCSAITDPMYKETYEALPIGYDFSGRE
HHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCHHHHHHHHHCCCCCCCCCHH
WFKKPMQSGDLHIMDVYQSHFTSKLIITVSCAVTDEKDNIAGVIGVDIQLEELLKRARSL
HHHHHHCCCCEEEEHHHHHHCCCEEEEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHH
KHEVGTVDDGD
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 9864346 [H]