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The map label for this gene is gyrB [H]

Identifier: 220903291

GI number: 220903291

Start: 5316

End: 7721

Strand: Direct

Name: gyrB [H]

Synonym: Ddes_0005

Alternate gene names: 220903291

Gene position: 5316-7721 (Clockwise)

Preceding gene: 220903290

Following gene: 220903292

Centisome position: 0.19

GC content: 56.86

Gene sequence:

>2406_bases
ATGGCTCTCCAAAACCCCGTCGACGGCGGCTACACCGCTTCTTCCATCACCATTCTGGAAGGGCTTTCGGCCGTTCGCAA
GCGCCCGGCCATGTATATAGGCTCAACGGACGCGCGCGGCCTGCACCATCTTGTTTACGAAGTGGTGGATAACTCCATTG
ACGAGGCCATGGCGGGCTTTTGCACGCGCATCACCGTCATTCTGCATGCCGACAACAGCGTTACCGTGCGTGACGATGGC
CGCGGCATTCCCGTTGATATCCACCCCAAGGAAGGCGTACCCGCCGTTCAGGTGGTCATGACAAAGCTGCATGCCGGCGG
CAAGTTTGACAATACAAGCTACAAGGTTTCCGGCGGCCTGCACGGCGTGGGCGTTTCCTGCGTCAACGCGCTTTCCGAAG
ACCTGACGGTCACTGTGCGCCGTGACGGTAAGCGGTACCGTCAGCGGTATTCCCGCGGTCTGCCGCAGGATGAGTTGACC
GTCATCGGTGAAGGCGTGGACGGCCACGGCACCACCGTGCGCTTCAAGCCTGACGAGGAAATTTTTGAAGTCTCAGAATT
TTCTTACGACACGTTGAAAAAACGCTTTGAAGAGCTGGCCTATCTCAACAAGGGCCTGTTCATCGAATGTATTGACGAGC
GCATCGGCGAAACACACGTGTTTCACGCCGAGGGCGGCATACGCCAGTTTGTGAGTGATCTTAATTCCGGCGAGCAGGGC
ATCCATTCGATTATCTTCGGTGAAGGCGTCGTGGACAATGTCACTGTGGATTTTGCCCTGCAATACAACGCTGGCTACAA
GGAAAACATCCTCACTTTCGCCAACAATATCCGCACCAAGGAAGGCGGAACCCACCTTGTGGGCTTTCGCACGGCCCTTA
CCCGCGCCATCAACGGCTATATCAAGGGGCAGGCCGACCTGGTCAAAAAGATGAAAAACACAAGCCTTTCCGGCGACGAC
GTGCGCGAAGGGCTTACGGCTGTCATCAGCGTCAAGCTGCCGCAGCCGCAGTTTGAAGGGCAGACCAAGACCAAGCTGGG
TAACAGCGAAGTGGCCGGTCTTGTGTCCGGCGTGGTGTATGATCGTCTGAACATCTATTTTGAAGAAAATCCCAAGGACA
TCAGGCTGATCATTGAAAAGGCCGTGGACGCTGCCCGCGCCCGCGATGCCGCCCGTCGCGCCAAGGAACTGGTACGCCGC
AAGGGCGCTCTTTCTGACAACGCCCTGCCCGGAAAGCTGGCAGACTGCCAGAGCAAGGATCCTTCGGAATCCGAACTGTT
CATCGTGGAAGGTGACTCGGCCGGCGGTTCTGCCAAGCAGGGGCGTAATCCCCGAAACCAGGCCATCCTGCCCCTGCGCG
GCAAGATCCTGAATACAGAGCGCACCCGCTTTGACAAGATGCTCGCCAACAAGGAAGTAAAGGCCCTTATCACGGCCATG
GGCGCTGGTATCGGTGAAGACGACACCGACCTTGAAAAGCTGCGTTACCACAAAATCATCATCATGACAGACGCCGACGT
GGACGGCGCACACATCCGTACCCTGCTGCTGACCTTCTTTTTTCGTCAGTATCAGGAGATGGTGGAGCGCGGTTTTGTCT
ATATTGCCCAGCCGCCGCTGTACCGCGTGCACAATTCGCGGATGGAAAAATTCATCAAGGACGATGCGGAGCTGAACAGC
TTCCTGCTTTCGCGCGTCAGCGAAGATGTCATCGTAACGGCGACTAACGGCAAGGAATACACGGGTACGGCCCTCAGCAA
CCTTGTGGAGCGCATTGAAACCCTTGAACTGCGTCTTGACGATGCTGAAATGACCGGGACTCCGCGTGATCTTTTTCTGG
CGCTCACCACGTATCCCGAACAGGTGGATGCGGCCATGCTTGAACGGCAGGACGCCGGACTTACAGAGTGGCTTAACGAG
CGCGGCTATATGCTCACACTGGAAACCGAGCAGAGCGAAGATGATGAAGAGCGCCTGTTTGCCATTTTCGAGAATACCGG
GGGCCACCAGTCGCGCCGTGGTATGGAATTCTTTGCCTCGCGGCTGTACCGCATGACCTGGCAGCTTTTTTCCGAGTTGC
GCGAGGAATGTGGCGGCCTGGACTTTGTCATGCGCCGCAAGGATGGCGAAACGCCCGCGCAAAACCTGTTTGATCTCATG
CGCATGGTGCTGGACGAAGCCCGCAAGGGCATCAATATCCAGCGCTACAAGGGCCTGGGTGAAATGAACCCGGAGCAGCT
CTGGGTAACCACTATGAATCCGGAAAACCGTATTCTGCTTCAGGTTTCTGTGGACGATGCCAACGAGGCCTCAGATGCGT
TTATGGAGCTTATGGGAGACCGCGTGGAGCCGCGCCGCGAATTCATCGAGCGCAACGCCCTGGCTGTACACGATCTGGAT
ATTTAG

Upstream 100 bases:

>100_bases
GCCCCTGCGGCATACGCGGGGCCGATGACCCGGACTACACCGTTATCATCATGCCCATGAAGGTTTCGGAAACGACATAT
TATAGCGAGGAAGACGTTTA

Downstream 100 bases:

>100_bases
GAAGGTTTTTTGGGCAAAGCAGGGGGGGGAGCCTTCAGCTCTGCTGTTCGCTTGGCGGCCGCCTTGCGGTCAGGAAAGCG
CCCTGCCGGAGAGTGCCGCA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 801; Mature: 800

Protein sequence:

>801_residues
MALQNPVDGGYTASSITILEGLSAVRKRPAMYIGSTDARGLHHLVYEVVDNSIDEAMAGFCTRITVILHADNSVTVRDDG
RGIPVDIHPKEGVPAVQVVMTKLHAGGKFDNTSYKVSGGLHGVGVSCVNALSEDLTVTVRRDGKRYRQRYSRGLPQDELT
VIGEGVDGHGTTVRFKPDEEIFEVSEFSYDTLKKRFEELAYLNKGLFIECIDERIGETHVFHAEGGIRQFVSDLNSGEQG
IHSIIFGEGVVDNVTVDFALQYNAGYKENILTFANNIRTKEGGTHLVGFRTALTRAINGYIKGQADLVKKMKNTSLSGDD
VREGLTAVISVKLPQPQFEGQTKTKLGNSEVAGLVSGVVYDRLNIYFEENPKDIRLIIEKAVDAARARDAARRAKELVRR
KGALSDNALPGKLADCQSKDPSESELFIVEGDSAGGSAKQGRNPRNQAILPLRGKILNTERTRFDKMLANKEVKALITAM
GAGIGEDDTDLEKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQYQEMVERGFVYIAQPPLYRVHNSRMEKFIKDDAELNS
FLLSRVSEDVIVTATNGKEYTGTALSNLVERIETLELRLDDAEMTGTPRDLFLALTTYPEQVDAAMLERQDAGLTEWLNE
RGYMLTLETEQSEDDEERLFAIFENTGGHQSRRGMEFFASRLYRMTWQLFSELREECGGLDFVMRRKDGETPAQNLFDLM
RMVLDEARKGINIQRYKGLGEMNPEQLWVTTMNPENRILLQVSVDDANEASDAFMELMGDRVEPRREFIERNALAVHDLD
I

Sequences:

>Translated_801_residues
MALQNPVDGGYTASSITILEGLSAVRKRPAMYIGSTDARGLHHLVYEVVDNSIDEAMAGFCTRITVILHADNSVTVRDDG
RGIPVDIHPKEGVPAVQVVMTKLHAGGKFDNTSYKVSGGLHGVGVSCVNALSEDLTVTVRRDGKRYRQRYSRGLPQDELT
VIGEGVDGHGTTVRFKPDEEIFEVSEFSYDTLKKRFEELAYLNKGLFIECIDERIGETHVFHAEGGIRQFVSDLNSGEQG
IHSIIFGEGVVDNVTVDFALQYNAGYKENILTFANNIRTKEGGTHLVGFRTALTRAINGYIKGQADLVKKMKNTSLSGDD
VREGLTAVISVKLPQPQFEGQTKTKLGNSEVAGLVSGVVYDRLNIYFEENPKDIRLIIEKAVDAARARDAARRAKELVRR
KGALSDNALPGKLADCQSKDPSESELFIVEGDSAGGSAKQGRNPRNQAILPLRGKILNTERTRFDKMLANKEVKALITAM
GAGIGEDDTDLEKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQYQEMVERGFVYIAQPPLYRVHNSRMEKFIKDDAELNS
FLLSRVSEDVIVTATNGKEYTGTALSNLVERIETLELRLDDAEMTGTPRDLFLALTTYPEQVDAAMLERQDAGLTEWLNE
RGYMLTLETEQSEDDEERLFAIFENTGGHQSRRGMEFFASRLYRMTWQLFSELREECGGLDFVMRRKDGETPAQNLFDLM
RMVLDEARKGINIQRYKGLGEMNPEQLWVTTMNPENRILLQVSVDDANEASDAFMELMGDRVEPRREFIERNALAVHDLD
I
>Mature_800_residues
ALQNPVDGGYTASSITILEGLSAVRKRPAMYIGSTDARGLHHLVYEVVDNSIDEAMAGFCTRITVILHADNSVTVRDDGR
GIPVDIHPKEGVPAVQVVMTKLHAGGKFDNTSYKVSGGLHGVGVSCVNALSEDLTVTVRRDGKRYRQRYSRGLPQDELTV
IGEGVDGHGTTVRFKPDEEIFEVSEFSYDTLKKRFEELAYLNKGLFIECIDERIGETHVFHAEGGIRQFVSDLNSGEQGI
HSIIFGEGVVDNVTVDFALQYNAGYKENILTFANNIRTKEGGTHLVGFRTALTRAINGYIKGQADLVKKMKNTSLSGDDV
REGLTAVISVKLPQPQFEGQTKTKLGNSEVAGLVSGVVYDRLNIYFEENPKDIRLIIEKAVDAARARDAARRAKELVRRK
GALSDNALPGKLADCQSKDPSESELFIVEGDSAGGSAKQGRNPRNQAILPLRGKILNTERTRFDKMLANKEVKALITAMG
AGIGEDDTDLEKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQYQEMVERGFVYIAQPPLYRVHNSRMEKFIKDDAELNSF
LLSRVSEDVIVTATNGKEYTGTALSNLVERIETLELRLDDAEMTGTPRDLFLALTTYPEQVDAAMLERQDAGLTEWLNER
GYMLTLETEQSEDDEERLFAIFENTGGHQSRRGMEFFASRLYRMTWQLFSELREECGGLDFVMRRKDGETPAQNLFDLMR
MVLDEARKGINIQRYKGLGEMNPEQLWVTTMNPENRILLQVSVDDANEASDAFMELMGDRVEPRREFIERNALAVHDLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=547, Percent_Identity=23.5831809872029, Blast_Score=110, Evalue=6e-24,
Organism=Homo sapiens, GI19913408, Length=536, Percent_Identity=23.6940298507463, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI48994957, Length=812, Percent_Identity=50.2463054187192, Blast_Score=730, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=536, Percent_Identity=44.2164179104478, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17535065, Length=532, Percent_Identity=24.6240601503759, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI212645845, Length=513, Percent_Identity=24.3664717348928, Blast_Score=120, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324241, Length=600, Percent_Identity=26.5, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17136538, Length=581, Percent_Identity=23.5800344234079, Blast_Score=112, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 89657; Mature: 89525

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALQNPVDGGYTASSITILEGLSAVRKRPAMYIGSTDARGLHHLVYEVVDNSIDEAMAGF
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCHHHHHHHHH
CTRITVILHADNSVTVRDDGRGIPVDIHPKEGVPAVQVVMTKLHAGGKFDNTSYKVSGGL
HEEEEEEEECCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGVSCVNALSEDLTVTVRRDGKRYRQRYSRGLPQDELTVIGEGVDGHGTTVRFKPDEE
CCHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCHH
IFEVSEFSYDTLKKRFEELAYLNKGLFIECIDERIGETHVFHAEGGIRQFVSDLNSGEQG
HHHHHHCCHHHHHHHHHHHHHHCCCCEEEEHHHHCCCEEEEECCCCHHHHHHHHCCCHHH
IHSIIFGEGVVDNVTVDFALQYNAGYKENILTFANNIRTKEGGTHLVGFRTALTRAINGY
HHHHHCCCCCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHH
IKGQADLVKKMKNTSLSGDDVREGLTAVISVKLPQPQFEGQTKTKLGNSEVAGLVSGVVY
CCCHHHHHHHHHCCCCCCHHHHHCCEEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHH
DRLNIYFEENPKDIRLIIEKAVDAARARDAARRAKELVRRKGALSDNALPGKLADCQSKD
HHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PSESELFIVEGDSAGGSAKQGRNPRNQAILPLRGKILNTERTRFDKMLANKEVKALITAM
CCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHH
GAGIGEDDTDLEKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQYQEMVERGFVYIAQPPL
CCCCCCCCCHHHHHCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCH
YRVHNSRMEKFIKDDAELNSFLLSRVSEDVIVTATNGKEYTGTALSNLVERIETLELRLD
HHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHEEEC
DAEMTGTPRDLFLALTTYPEQVDAAMLERQDAGLTEWLNERGYMLTLETEQSEDDEERLF
CCCCCCCCHHEEEEEECCCHHHHHHHHHHCCCCHHHHHHCCCEEEEEECCCCCCCHHHEE
AIFENTGGHQSRRGMEFFASRLYRMTWQLFSELREECGGLDFVMRRKDGETPAQNLFDLM
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
RMVLDEARKGINIQRYKGLGEMNPEQLWVTTMNPENRILLQVSVDDANEASDAFMELMGD
HHHHHHHHHCCCCCEECCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHCC
RVEPRREFIERNALAVHDLDI
CCCHHHHHHHCCCEEEEECCC
>Mature Secondary Structure 
ALQNPVDGGYTASSITILEGLSAVRKRPAMYIGSTDARGLHHLVYEVVDNSIDEAMAGF
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCHHHHHHHHH
CTRITVILHADNSVTVRDDGRGIPVDIHPKEGVPAVQVVMTKLHAGGKFDNTSYKVSGGL
HEEEEEEEECCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGVSCVNALSEDLTVTVRRDGKRYRQRYSRGLPQDELTVIGEGVDGHGTTVRFKPDEE
CCHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCHH
IFEVSEFSYDTLKKRFEELAYLNKGLFIECIDERIGETHVFHAEGGIRQFVSDLNSGEQG
HHHHHHCCHHHHHHHHHHHHHHCCCCEEEEHHHHCCCEEEEECCCCHHHHHHHHCCCHHH
IHSIIFGEGVVDNVTVDFALQYNAGYKENILTFANNIRTKEGGTHLVGFRTALTRAINGY
HHHHHCCCCCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHH
IKGQADLVKKMKNTSLSGDDVREGLTAVISVKLPQPQFEGQTKTKLGNSEVAGLVSGVVY
CCCHHHHHHHHHCCCCCCHHHHHCCEEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHH
DRLNIYFEENPKDIRLIIEKAVDAARARDAARRAKELVRRKGALSDNALPGKLADCQSKD
HHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PSESELFIVEGDSAGGSAKQGRNPRNQAILPLRGKILNTERTRFDKMLANKEVKALITAM
CCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHH
GAGIGEDDTDLEKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQYQEMVERGFVYIAQPPL
CCCCCCCCCHHHHHCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCH
YRVHNSRMEKFIKDDAELNSFLLSRVSEDVIVTATNGKEYTGTALSNLVERIETLELRLD
HHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHEEEC
DAEMTGTPRDLFLALTTYPEQVDAAMLERQDAGLTEWLNERGYMLTLETEQSEDDEERLF
CCCCCCCCHHEEEEEECCCHHHHHHHHHHCCCCHHHHHHCCCEEEEEECCCCCCCHHHEE
AIFENTGGHQSRRGMEFFASRLYRMTWQLFSELREECGGLDFVMRRKDGETPAQNLFDLM
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
RMVLDEARKGINIQRYKGLGEMNPEQLWVTTMNPENRILLQVSVDDANEASDAFMELMGD
HHHHHHHHHCCCCCEECCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHCC
RVEPRREFIERNALAVHDLDI
CCCHHHHHHHCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]