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The map label for this gene is dnaN [H]

Identifier: 220903290

GI number: 220903290

Start: 4162

End: 5316

Strand: Direct

Name: dnaN [H]

Synonym: Ddes_0004

Alternate gene names: 220903290

Gene position: 4162-5316 (Clockwise)

Preceding gene: 220903289

Following gene: 220903291

Centisome position: 0.14

GC content: 56.45

Gene sequence:

>1155_bases
ATGAAACTTACTGTTAATAAAGAGCAGATCATCGAAGGGCTGTTGAAGGCTGTTGCCATTATTCCCGCCAAGGCAGGGGC
GCAGTATCTGCGTTCCATCTGGCTCAAGGCGGAAGACGGCGGCCTTTCGGTCATGTCTACTGATGCCAACATCGAGTTTA
CGGGGCGTTACCAGGCAGAAGTGGCACAACCCGGGCTGATCGGCGTACAGGGCAGGGCCTTTGTGGATCTGGTACGCCAG
CTGCCTTCAGGCGTGCTGCATCTTTCCCTTGACGAGGCTTCGGGCAACCTGCTGGTAGAGCAGGGTCGCCGCACGTACAA
GCTGCCTGTGAGCGGCGCCGAGTGGTTTCAGAACTTTTCCAGTTTTCCTGAAGAGAACACGGTGACCTGGTCCGGCGACT
TTCTTCAGGACCTTTTGGACAAGGTGGCCTTTTGCATCAGCGATGACGATGCTATGGATGCCATTGCCTGCCTGTGCATG
ATGCCCAAGGGCAACGGGCGTATAGACGTCTGCGGGCTTAACGGCCACCAGTTTGCCCTTGTTTCCTTTACGCACGACGA
ACTTGCCGAGCGCCTGCCTGAAGAGGGCATTCTCATCCAGAAAAAGTATCTTCAGGATATCAAGAAGTGGCTGGGCGTGG
ATGAGATTGAACTGAGCATCACCGGAAAGCGCCTGTACCTGCGCAGCATGAGCAAGGCCGAAACCCTGAGCCTGCCCCGG
GCCACGCATCAGTATCCCGACTACAGCATCTTCATGAGCAAGCTGGCCAGCGAAGACATGCATCCCATGACGCTTGACCG
CAAAGAAGCCATGGAGGCCCTTGGCCGTATTCTTATTTTCAATACGGAAAGCGACCGCTGCACCTATATGGACCTTTCCT
CCGGCGAAGCCCTGCTTTCCGCCCAGGGGCAGGACGTCGGCTCGGCCAGCGAAAGCCTGGAAGTAACCTACGGCGGCGAC
ATCAAGCGCATCGCCTTTCCCACCCGCAACCTTATGGACGTGCTGGCTCACTTTGTGTCCGGCAAGACAGACATGATGCT
TACGGGCGCTGAAGGCCCCTGCGGCATACGCGGGGCCGATGACCCGGACTACACCGTTATCATCATGCCCATGAAGGTTT
CGGAAACGACATATTATAGCGAGGAAGACGTTTAA

Upstream 100 bases:

>100_bases
AAAACACGTCGGGCTGTTCCATAAAAAATTCAATCTTTACAAGGCGTTTAAGCCGATACGAACATAGAAACACTGCCAAT
AATAGTTAAGGAAAGAATGT

Downstream 100 bases:

>100_bases
TGGCTCTCCAAAACCCCGTCGACGGCGGCTACACCGCTTCTTCCATCACCATTCTGGAAGGGCTTTCGGCCGTTCGCAAG
CGCCCGGCCATGTATATAGG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MKLTVNKEQIIEGLLKAVAIIPAKAGAQYLRSIWLKAEDGGLSVMSTDANIEFTGRYQAEVAQPGLIGVQGRAFVDLVRQ
LPSGVLHLSLDEASGNLLVEQGRRTYKLPVSGAEWFQNFSSFPEENTVTWSGDFLQDLLDKVAFCISDDDAMDAIACLCM
MPKGNGRIDVCGLNGHQFALVSFTHDELAERLPEEGILIQKKYLQDIKKWLGVDEIELSITGKRLYLRSMSKAETLSLPR
ATHQYPDYSIFMSKLASEDMHPMTLDRKEAMEALGRILIFNTESDRCTYMDLSSGEALLSAQGQDVGSASESLEVTYGGD
IKRIAFPTRNLMDVLAHFVSGKTDMMLTGAEGPCGIRGADDPDYTVIIMPMKVSETTYYSEEDV

Sequences:

>Translated_384_residues
MKLTVNKEQIIEGLLKAVAIIPAKAGAQYLRSIWLKAEDGGLSVMSTDANIEFTGRYQAEVAQPGLIGVQGRAFVDLVRQ
LPSGVLHLSLDEASGNLLVEQGRRTYKLPVSGAEWFQNFSSFPEENTVTWSGDFLQDLLDKVAFCISDDDAMDAIACLCM
MPKGNGRIDVCGLNGHQFALVSFTHDELAERLPEEGILIQKKYLQDIKKWLGVDEIELSITGKRLYLRSMSKAETLSLPR
ATHQYPDYSIFMSKLASEDMHPMTLDRKEAMEALGRILIFNTESDRCTYMDLSSGEALLSAQGQDVGSASESLEVTYGGD
IKRIAFPTRNLMDVLAHFVSGKTDMMLTGAEGPCGIRGADDPDYTVIIMPMKVSETTYYSEEDV
>Mature_384_residues
MKLTVNKEQIIEGLLKAVAIIPAKAGAQYLRSIWLKAEDGGLSVMSTDANIEFTGRYQAEVAQPGLIGVQGRAFVDLVRQ
LPSGVLHLSLDEASGNLLVEQGRRTYKLPVSGAEWFQNFSSFPEENTVTWSGDFLQDLLDKVAFCISDDDAMDAIACLCM
MPKGNGRIDVCGLNGHQFALVSFTHDELAERLPEEGILIQKKYLQDIKKWLGVDEIELSITGKRLYLRSMSKAETLSLPR
ATHQYPDYSIFMSKLASEDMHPMTLDRKEAMEALGRILIFNTESDRCTYMDLSSGEALLSAQGQDVGSASESLEVTYGGD
IKRIAFPTRNLMDVLAHFVSGKTDMMLTGAEGPCGIRGADDPDYTVIIMPMKVSETTYYSEEDV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=374, Percent_Identity=22.4598930481283, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42397; Mature: 42397

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTVNKEQIIEGLLKAVAIIPAKAGAQYLRSIWLKAEDGGLSVMSTDANIEFTGRYQAE
CEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEECCCCEEEEEECCCEEEECCEEEH
VAQPGLIGVQGRAFVDLVRQLPSGVLHLSLDEASGNLLVEQGRRTYKLPVSGAEWFQNFS
HCCCCEEEECCHHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCEEEECCCHHHHHHHHH
SFPEENTVTWSGDFLQDLLDKVAFCISDDDAMDAIACLCMMPKGNGRIDVCGLNGHQFAL
CCCCCCEEEECHHHHHHHHHHHHEECCCCHHHHHHHHHEECCCCCCEEEEEECCCCEEEE
VSFTHDELAERLPEEGILIQKKYLQDIKKWLGVDEIELSITGKRLYLRSMSKAETLSLPR
EEECHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCEEEEEEECCEEEEHHCCCHHHHCCCC
ATHQYPDYSIFMSKLASEDMHPMTLDRKEAMEALGRILIFNTESDRCTYMDLSSGEALLS
HHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCEEEEEECCCCCEEEEECCCCCEEEE
AQGQDVGSASESLEVTYGGDIKRIAFPTRNLMDVLAHFVSGKTDMMLTGAEGPCGIRGAD
CCCCCCCCCCCCEEEEECCCEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
DPDYTVIIMPMKVSETTYYSEEDV
CCCEEEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKLTVNKEQIIEGLLKAVAIIPAKAGAQYLRSIWLKAEDGGLSVMSTDANIEFTGRYQAE
CEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEECCCCEEEEEECCCEEEECCEEEH
VAQPGLIGVQGRAFVDLVRQLPSGVLHLSLDEASGNLLVEQGRRTYKLPVSGAEWFQNFS
HCCCCEEEECCHHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCEEEECCCHHHHHHHHH
SFPEENTVTWSGDFLQDLLDKVAFCISDDDAMDAIACLCMMPKGNGRIDVCGLNGHQFAL
CCCCCCEEEECHHHHHHHHHHHHEECCCCHHHHHHHHHEECCCCCCEEEEEECCCCEEEE
VSFTHDELAERLPEEGILIQKKYLQDIKKWLGVDEIELSITGKRLYLRSMSKAETLSLPR
EEECHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCEEEEEEECCEEEEHHCCCHHHHCCCC
ATHQYPDYSIFMSKLASEDMHPMTLDRKEAMEALGRILIFNTESDRCTYMDLSSGEALLS
HHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCEEEEEECCCCCEEEEECCCCCEEEE
AQGQDVGSASESLEVTYGGDIKRIAFPTRNLMDVLAHFVSGKTDMMLTGAEGPCGIRGAD
CCCCCCCCCCCCEEEEECCCEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
DPDYTVIIMPMKVSETTYYSEEDV
CCCEEEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]