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The map label for this gene is thsB [H]

Identifier: 218884572

GI number: 218884572

Start: 1179228

End: 1180877

Strand: Reverse

Name: thsB [H]

Synonym: DKAM_1261

Alternate gene names: 218884572

Gene position: 1180877-1179228 (Counterclockwise)

Preceding gene: 218884573

Following gene: 218884570

Centisome position: 86.5

GC content: 45.94

Gene sequence:

>1650_bases
ATGACAGCTGAACCCACAGGCATACCTGTACTAATATTAAAAGAGGGAACGCAGCGTACAACGGGCAGGGATGCCCTAAG
ATCCAATCTTCTAGCCGCTAGAGCTATAGCTGAAATGATTAAGACGACCTATGGTCCTAAAGGAATGGATAAAATGCTTG
TCGATGCCCTCGGCGATGTAACCATAACAAATGACGGCGCTACAATCCTCGATAAGGCGGAGATACAGCATCCAGCTGCG
AAGATGCTTGTTCAGGTAGCCAAGAGCCAGGACAGTGAAGTAGGTGATGGTACAAAGCGTGCCGTGATTTTCGCTGGAGA
GTTACTGAGATACGCTGAAGAACTACTCGATAAAAATATTCACCCAACAGTAATAATCTCCGGCTATAGAATAGCTATGG
AAGAGGCACTTAAAGTACTAGACCAGATAGCTGAGCCAATAGATATAAACAGTGAAGAATTACTGAAGAAGGTTGCGAGA
ACATCACTCACAAGTAAAGCCGTTCATGATGCCCGTGAATTCTTCGCCGACATAGCTGTTAAAGCAGTTAAACAGGTTGT
TGAGAAGAGAGGGGACAAGTACTACGTAGACCTCGATAACATCCAGATAATAAAGAAGTATGGTGGAGCCCTACTAGACT
CAATGCTTGTCTACGGCATCGTGTTGGATAAGGAGGTTGTCCACCCGGGCATGCCTAGGAGAGTTGAGAACGCTAGGATA
GTGCTCCTAGATGCCCCATTAGAGATAGAAAAGCCTGAGATAGATGCCGAGATAAGAATAAATGATCCAGAGCAATTAGA
GAAATTCCTGCAACAGGAAGAAGATATACTGGTTAAGATGGTTGACAAGATAGCTAGCGTCGGCGCTAACGTGGTTGTAT
GCCAGAAAGGCATTGATGAGGTTGCCCAGCACTTCCTAGCTAAGAAGGGGATATTAGCCGTTAGAAGAGTTAAGAGGAGC
GACCTCGAGAAACTCGAGAGGGCTACAGGCGGCAGAATAGTAAGCAACATAGAGGACTTAACACCAGAGGACCTCGGATA
CGCTGCACTAGTAGAGGAGAGGAAGATTGGAGAAGATAAAATGGTATTCATAGAGGGATGCAAGAACCCGAGATCCGTAA
GCATAGTGATAAGGGGAGGATTAGAGAGGCTGGTTGATGAAGCTGAGAGAAGCATGAGAGATGCGTTATCAGCTGTAGCG
GACGCATTAAGAGACGGTAAGATTATTCCAGGCGGTGGAGCAACAGAGATCGAGCTAGCTAAATACATAAGAAGGCTTGC
AACGAAGATCGGTGGTAAGGAACAGTTAGCAATAGAGGCGTTCGCGAAAGCCATTGAGGGGCTAACAGTATCACTGATTG
AGAATGCTGGATTAGACCCAATAGACATGATAATGAAGCTTAGAGCTGCCCATGAGAAGGATAGCGGTAAATACTTAAGC
ATAGACCTCGCAACAGGCGACCTGGTAAACATGAAGGAGAAAGGTGTCATAGAGCCAGTGAGCATACTGGCTAACGCGAT
AAAAGCGGGTACTGAAGCAGCCACCATAATATTGAGAATAGATGACGTGATAGCGGCCAGTAAGCTCGAGAAGGGCAAAG
AGGAAACCGGTAAGAAACCCGGCGAAGAAGAAAAAGAGAAAGAAGACTAA

Upstream 100 bases:

>100_bases
GCACCCATCATCGAGATCTTTAAAATAGTTATGATAGAAAAGTTTATATAGAAGTCCTTTCATGAAATACCATTGGATTT
TATCACACCTGGTGATTGCT

Downstream 100 bases:

>100_bases
ACTTATTTTTGAAGATTTAAACTACTTAACCGTTAATCCCAAATAGATTTTATCCATGGGCCTTAAATCCAGTTGGCTGG
GGCTTTTTATAACAACTAGT

Product: Thermosome subunit beta

Products: NA

Alternate protein names: Chaperonin subunit beta; Thermosome subunit 2 [H]

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MTAEPTGIPVLILKEGTQRTTGRDALRSNLLAARAIAEMIKTTYGPKGMDKMLVDALGDVTITNDGATILDKAEIQHPAA
KMLVQVAKSQDSEVGDGTKRAVIFAGELLRYAEELLDKNIHPTVIISGYRIAMEEALKVLDQIAEPIDINSEELLKKVAR
TSLTSKAVHDAREFFADIAVKAVKQVVEKRGDKYYVDLDNIQIIKKYGGALLDSMLVYGIVLDKEVVHPGMPRRVENARI
VLLDAPLEIEKPEIDAEIRINDPEQLEKFLQQEEDILVKMVDKIASVGANVVVCQKGIDEVAQHFLAKKGILAVRRVKRS
DLEKLERATGGRIVSNIEDLTPEDLGYAALVEERKIGEDKMVFIEGCKNPRSVSIVIRGGLERLVDEAERSMRDALSAVA
DALRDGKIIPGGGATEIELAKYIRRLATKIGGKEQLAIEAFAKAIEGLTVSLIENAGLDPIDMIMKLRAAHEKDSGKYLS
IDLATGDLVNMKEKGVIEPVSILANAIKAGTEAATIILRIDDVIAASKLEKGKEETGKKPGEEEKEKED

Sequences:

>Translated_549_residues
MTAEPTGIPVLILKEGTQRTTGRDALRSNLLAARAIAEMIKTTYGPKGMDKMLVDALGDVTITNDGATILDKAEIQHPAA
KMLVQVAKSQDSEVGDGTKRAVIFAGELLRYAEELLDKNIHPTVIISGYRIAMEEALKVLDQIAEPIDINSEELLKKVAR
TSLTSKAVHDAREFFADIAVKAVKQVVEKRGDKYYVDLDNIQIIKKYGGALLDSMLVYGIVLDKEVVHPGMPRRVENARI
VLLDAPLEIEKPEIDAEIRINDPEQLEKFLQQEEDILVKMVDKIASVGANVVVCQKGIDEVAQHFLAKKGILAVRRVKRS
DLEKLERATGGRIVSNIEDLTPEDLGYAALVEERKIGEDKMVFIEGCKNPRSVSIVIRGGLERLVDEAERSMRDALSAVA
DALRDGKIIPGGGATEIELAKYIRRLATKIGGKEQLAIEAFAKAIEGLTVSLIENAGLDPIDMIMKLRAAHEKDSGKYLS
IDLATGDLVNMKEKGVIEPVSILANAIKAGTEAATIILRIDDVIAASKLEKGKEETGKKPGEEEKEKED
>Mature_548_residues
TAEPTGIPVLILKEGTQRTTGRDALRSNLLAARAIAEMIKTTYGPKGMDKMLVDALGDVTITNDGATILDKAEIQHPAAK
MLVQVAKSQDSEVGDGTKRAVIFAGELLRYAEELLDKNIHPTVIISGYRIAMEEALKVLDQIAEPIDINSEELLKKVART
SLTSKAVHDAREFFADIAVKAVKQVVEKRGDKYYVDLDNIQIIKKYGGALLDSMLVYGIVLDKEVVHPGMPRRVENARIV
LLDAPLEIEKPEIDAEIRINDPEQLEKFLQQEEDILVKMVDKIASVGANVVVCQKGIDEVAQHFLAKKGILAVRRVKRSD
LEKLERATGGRIVSNIEDLTPEDLGYAALVEERKIGEDKMVFIEGCKNPRSVSIVIRGGLERLVDEAERSMRDALSAVAD
ALRDGKIIPGGGATEIELAKYIRRLATKIGGKEQLAIEAFAKAIEGLTVSLIENAGLDPIDMIMKLRAAHEKDSGKYLSI
DLATGDLVNMKEKGVIEPVSILANAIKAGTEAATIILRIDDVIAASKLEKGKEETGKKPGEEEKEKED

Specific function: Molecular chaperone; binds unfolded polypeptides in vitro, and has a weak ATPase activity [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TCP-1 chaperonin family [H]

Homologues:

Organism=Homo sapiens, GI63162572, Length=530, Percent_Identity=37.5471698113208, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI24307939, Length=545, Percent_Identity=39.8165137614679, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI57863257, Length=525, Percent_Identity=40.3809523809524, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI5453607, Length=527, Percent_Identity=37.5711574952562, Blast_Score=356, Evalue=3e-98,
Organism=Homo sapiens, GI38455427, Length=507, Percent_Identity=39.2504930966469, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI58761484, Length=530, Percent_Identity=34.1509433962264, Blast_Score=347, Evalue=2e-95,
Organism=Homo sapiens, GI261399877, Length=483, Percent_Identity=36.4389233954451, Blast_Score=316, Evalue=4e-86,
Organism=Homo sapiens, GI4502643, Length=512, Percent_Identity=35.7421875, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI58331173, Length=507, Percent_Identity=35.8974358974359, Blast_Score=306, Evalue=4e-83,
Organism=Homo sapiens, GI5453603, Length=527, Percent_Identity=34.5351043643264, Blast_Score=299, Evalue=4e-81,
Organism=Homo sapiens, GI261399875, Length=438, Percent_Identity=33.7899543378995, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI302058290, Length=507, Percent_Identity=33.5305719921105, Blast_Score=259, Evalue=5e-69,
Organism=Homo sapiens, GI58331171, Length=512, Percent_Identity=32.421875, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI48762932, Length=539, Percent_Identity=33.0241187384045, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI302058292, Length=507, Percent_Identity=32.5443786982249, Blast_Score=246, Evalue=4e-65,
Organism=Homo sapiens, GI57863259, Length=377, Percent_Identity=36.0742705570292, Blast_Score=238, Evalue=2e-62,
Organism=Homo sapiens, GI58331185, Length=317, Percent_Identity=34.384858044164, Blast_Score=208, Evalue=1e-53,
Organism=Homo sapiens, GI7657253, Length=534, Percent_Identity=26.5917602996255, Blast_Score=169, Evalue=9e-42,
Organism=Homo sapiens, GI25914754, Length=444, Percent_Identity=22.5225225225225, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI9055272, Length=444, Percent_Identity=22.5225225225225, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI121583483, Length=216, Percent_Identity=30.5555555555556, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI41399285, Length=532, Percent_Identity=22.1804511278195, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI31542947, Length=532, Percent_Identity=22.1804511278195, Blast_Score=69, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25144674, Length=522, Percent_Identity=40.0383141762452, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17532603, Length=507, Percent_Identity=39.6449704142012, Blast_Score=358, Evalue=4e-99,
Organism=Caenorhabditis elegans, GI17532601, Length=537, Percent_Identity=38.7337057728119, Blast_Score=355, Evalue=5e-98,
Organism=Caenorhabditis elegans, GI25148561, Length=537, Percent_Identity=34.6368715083799, Blast_Score=345, Evalue=5e-95,
Organism=Caenorhabditis elegans, GI17564182, Length=544, Percent_Identity=35.2941176470588, Blast_Score=320, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI25147750, Length=528, Percent_Identity=36.5530303030303, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI25144678, Length=520, Percent_Identity=33.6538461538462, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI32566944, Length=445, Percent_Identity=34.8314606741573, Blast_Score=278, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI25144680, Length=397, Percent_Identity=35.264483627204, Blast_Score=245, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI71998178, Length=573, Percent_Identity=30.5410122164049, Blast_Score=234, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI71981457, Length=325, Percent_Identity=37.2307692307692, Blast_Score=215, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17555558, Length=561, Percent_Identity=23.1729055258467, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI212650582, Length=179, Percent_Identity=25.1396648044693, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322446, Length=531, Percent_Identity=37.8531073446328, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6320418, Length=532, Percent_Identity=37.781954887218, Blast_Score=349, Evalue=8e-97,
Organism=Saccharomyces cerevisiae, GI6322524, Length=538, Percent_Identity=36.8029739776952, Blast_Score=348, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6322350, Length=523, Percent_Identity=36.3288718929254, Blast_Score=343, Evalue=3e-95,
Organism=Saccharomyces cerevisiae, GI6320058, Length=509, Percent_Identity=36.3457760314342, Blast_Score=313, Evalue=5e-86,
Organism=Saccharomyces cerevisiae, GI6320393, Length=523, Percent_Identity=34.9904397705545, Blast_Score=310, Evalue=5e-85,
Organism=Saccharomyces cerevisiae, GI6322049, Length=523, Percent_Identity=34.7992351816444, Blast_Score=284, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6322452, Length=556, Percent_Identity=30.3956834532374, Blast_Score=253, Evalue=8e-68,
Organism=Drosophila melanogaster, GI17647245, Length=523, Percent_Identity=39.3881453154876, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24649027, Length=522, Percent_Identity=38.8888888888889, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24649029, Length=522, Percent_Identity=38.8888888888889, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24652903, Length=500, Percent_Identity=39.2, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24645179, Length=526, Percent_Identity=37.6425855513308, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24647512, Length=534, Percent_Identity=35.2059925093633, Blast_Score=345, Evalue=6e-95,
Organism=Drosophila melanogaster, GI24647510, Length=534, Percent_Identity=35.2059925093633, Blast_Score=345, Evalue=6e-95,
Organism=Drosophila melanogaster, GI28571140, Length=525, Percent_Identity=36.5714285714286, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI18858175, Length=525, Percent_Identity=36.5714285714286, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24583944, Length=510, Percent_Identity=37.4509803921569, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI18859933, Length=512, Percent_Identity=35.546875, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI19921848, Length=523, Percent_Identity=32.6959847036329, Blast_Score=262, Evalue=5e-70,
Organism=Drosophila melanogaster, GI20130093, Length=187, Percent_Identity=32.620320855615, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI45552711, Length=187, Percent_Identity=32.620320855615, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI45550936, Length=535, Percent_Identity=24.1121495327103, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI45550132, Length=535, Percent_Identity=24.1121495327103, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI45550935, Length=535, Percent_Identity=24.1121495327103, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017998
- InterPro:   IPR002194
- InterPro:   IPR002423
- InterPro:   IPR012714 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 60003; Mature: 59872

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00750 TCP1_1 ; PS00751 TCP1_2 ; PS00995 TCP1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEPTGIPVLILKEGTQRTTGRDALRSNLLAARAIAEMIKTTYGPKGMDKMLVDALGDV
CCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCE
TITNDGATILDKAEIQHPAAKMLVQVAKSQDSEVGDGTKRAVIFAGELLRYAEELLDKNI
EEECCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
HPTVIISGYRIAMEEALKVLDQIAEPIDINSEELLKKVARTSLTSKAVHDAREFFADIAV
CCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAVKQVVEKRGDKYYVDLDNIQIIKKYGGALLDSMLVYGIVLDKEVVHPGMPRRVENARI
HHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
VLLDAPLEIEKPEIDAEIRINDPEQLEKFLQQEEDILVKMVDKIASVGANVVVCQKGIDE
EEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHH
VAQHFLAKKGILAVRRVKRSDLEKLERATGGRIVSNIEDLTPEDLGYAALVEERKIGEDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
MVFIEGCKNPRSVSIVIRGGLERLVDEAERSMRDALSAVADALRDGKIIPGGGATEIELA
EEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
KYIRRLATKIGGKEQLAIEAFAKAIEGLTVSLIENAGLDPIDMIMKLRAAHEKDSGKYLS
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEE
IDLATGDLVNMKEKGVIEPVSILANAIKAGTEAATIILRIDDVIAASKLEKGKEETGKKP
EEECCCCCCCCHHCCCCCHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHCCHHHHCCCC
GEEEKEKED
CCCCCCCCC
>Mature Secondary Structure 
TAEPTGIPVLILKEGTQRTTGRDALRSNLLAARAIAEMIKTTYGPKGMDKMLVDALGDV
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCE
TITNDGATILDKAEIQHPAAKMLVQVAKSQDSEVGDGTKRAVIFAGELLRYAEELLDKNI
EEECCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
HPTVIISGYRIAMEEALKVLDQIAEPIDINSEELLKKVARTSLTSKAVHDAREFFADIAV
CCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAVKQVVEKRGDKYYVDLDNIQIIKKYGGALLDSMLVYGIVLDKEVVHPGMPRRVENARI
HHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
VLLDAPLEIEKPEIDAEIRINDPEQLEKFLQQEEDILVKMVDKIASVGANVVVCQKGIDE
EEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHH
VAQHFLAKKGILAVRRVKRSDLEKLERATGGRIVSNIEDLTPEDLGYAALVEERKIGEDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
MVFIEGCKNPRSVSIVIRGGLERLVDEAERSMRDALSAVADALRDGKIIPGGGATEIELA
EEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
KYIRRLATKIGGKEQLAIEAFAKAIEGLTVSLIENAGLDPIDMIMKLRAAHEKDSGKYLS
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEE
IDLATGDLVNMKEKGVIEPVSILANAIKAGTEAATIILRIDDVIAASKLEKGKEETGKKP
EEECCCCCCCCHHCCCCCHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHCCHHHHCCCC
GEEEKEKED
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10508614 [H]