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The map label for this gene is aspS [H]

Identifier: 218884222

GI number: 218884222

Start: 864827

End: 866089

Strand: Reverse

Name: aspS [H]

Synonym: DKAM_0911

Alternate gene names: 218884222

Gene position: 866089-864827 (Counterclockwise)

Preceding gene: 218884223

Following gene: 218884221

Centisome position: 63.44

GC content: 46.4

Gene sequence:

>1263_bases
ATGGGTTTAAAGAGGATTTGTGGTTGGATTATAAGGAAGAAAAGTATTGGAAGGATAATTATTATCGAGGTAAGTAGCGA
CAGCGTGAAACCAAGTGTCCTGGTATTAAAGGAGGAAAGGGAGCCTGAATTATTTAAGCTGGGGATGGAAATCGATATCG
GCACAGCTCTCTGCTTCGAGGGGGAGGAGGCCCCAGAGCAAAGGAGTGCGAGAGGAGTAGAGTATATAGCTAGGAAGATA
GAGGTTTACGCGAAACCCATTGAACCCCTCCCAGTCGACACCATGGGTAAGGTACCGGCTTTACTCGATACGAGGATAAA
GTACAGATGGCTCTTAGTGAGAAACCCCGTTGAGAAGGCGATATTCATTATTAGGAATAGCTTACTCAATGCAGCCAGAG
AGTATTTCTCCAAGAACGGGTTCATAGAGGTTCAAACCCCGAAGATCGTGGCTGCTGGTGCTGAAGGCGGTGCAACACTG
TTTAAGATCCAGTATTTCGAGAACGATGCGTATCTTAGCCAGAGTCCCCAGTTATTTAAACAAATGTTGATGGCTGGCTT
CACAAGGATCTATGAGATAACCCCGTATTTCAGGGCTGAGAAATACAATACGACGAGACACCTGAACGAGTCATGGGGTA
TAGATGTGGAGCAAGGCTTTATAAATAGCGTCGAGGATGTTCTCCAAACGCTTGAAAACCTGATTACATACATCATAGAG
TATGTGTTGAAGAACAATAAAGAGGAGCTGGAAATACTCGGCGCCACGCTTAAGAAACCCTCGTCACCGTTTAAGAGGCT
AAAATTCCAGGAGGCAGTCGATATACTTAGAAGCGAAGGACTCGAGATCTCGGAGGCAGAGGACCTAAGCGACTACGCTG
AGAAGAAGCTGGGAGAAATAATGACCGAGAAGGGATATGAGCTATACTTTATAGTGGGATTCCCATGGGCTTCAACGGGC
TTCTACTACATGAGGGATGAGGACGGGGTCTACACTAGGAAATTCGATCTCGATTACCGCGGGCTGGAGATAGCGAGTGG
AGGACAAAGGGAGCATAGGTATGAGAAGCTATTGAAAGCGTTGAGGGAGAAAGGTCTTAACCCGGAGAACTTCGGCTTTT
ACCTGGAGGCTTTCAAATATGGTATGCCGCCACATGGCGGGTTCGGGCTAGGTGTTGAGAGATTATTGATGAGAATGCTC
AACCTGGAAAACGTTAGGGAGGCCATATTATTTGTTAGGGATAGAACAAGACTGGTGCCTTAA

Upstream 100 bases:

>100_bases
AATAAAATATTATTTTAAACCTACTAATAAATTTTTACAGCTAGAAGCCTGCCCTTATAGAGCTGAGAAGGCTGGGACAT
GCTTTCACAGGTGAGGTAGA

Downstream 100 bases:

>100_bases
CTTTTTTTGACACCATTTCTCCCTAGGTGTTCGCTCTAATTATACTATGTTCATGGTTTCACCTAGGGGACTCCACCCAG
CCCACCAACCCATATAAACC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MGLKRICGWIIRKKSIGRIIIIEVSSDSVKPSVLVLKEEREPELFKLGMEIDIGTALCFEGEEAPEQRSARGVEYIARKI
EVYAKPIEPLPVDTMGKVPALLDTRIKYRWLLVRNPVEKAIFIIRNSLLNAAREYFSKNGFIEVQTPKIVAAGAEGGATL
FKIQYFENDAYLSQSPQLFKQMLMAGFTRIYEITPYFRAEKYNTTRHLNESWGIDVEQGFINSVEDVLQTLENLITYIIE
YVLKNNKEELEILGATLKKPSSPFKRLKFQEAVDILRSEGLEISEAEDLSDYAEKKLGEIMTEKGYELYFIVGFPWASTG
FYYMRDEDGVYTRKFDLDYRGLEIASGGQREHRYEKLLKALREKGLNPENFGFYLEAFKYGMPPHGGFGLGVERLLMRML
NLENVREAILFVRDRTRLVP

Sequences:

>Translated_420_residues
MGLKRICGWIIRKKSIGRIIIIEVSSDSVKPSVLVLKEEREPELFKLGMEIDIGTALCFEGEEAPEQRSARGVEYIARKI
EVYAKPIEPLPVDTMGKVPALLDTRIKYRWLLVRNPVEKAIFIIRNSLLNAAREYFSKNGFIEVQTPKIVAAGAEGGATL
FKIQYFENDAYLSQSPQLFKQMLMAGFTRIYEITPYFRAEKYNTTRHLNESWGIDVEQGFINSVEDVLQTLENLITYIIE
YVLKNNKEELEILGATLKKPSSPFKRLKFQEAVDILRSEGLEISEAEDLSDYAEKKLGEIMTEKGYELYFIVGFPWASTG
FYYMRDEDGVYTRKFDLDYRGLEIASGGQREHRYEKLLKALREKGLNPENFGFYLEAFKYGMPPHGGFGLGVERLLMRML
NLENVREAILFVRDRTRLVP
>Mature_419_residues
GLKRICGWIIRKKSIGRIIIIEVSSDSVKPSVLVLKEEREPELFKLGMEIDIGTALCFEGEEAPEQRSARGVEYIARKIE
VYAKPIEPLPVDTMGKVPALLDTRIKYRWLLVRNPVEKAIFIIRNSLLNAAREYFSKNGFIEVQTPKIVAAGAEGGATLF
KIQYFENDAYLSQSPQLFKQMLMAGFTRIYEITPYFRAEKYNTTRHLNESWGIDVEQGFINSVEDVLQTLENLITYIIEY
VLKNNKEELEILGATLKKPSSPFKRLKFQEAVDILRSEGLEISEAEDLSDYAEKKLGEIMTEKGYELYFIVGFPWASTGF
YYMRDEDGVYTRKFDLDYRGLEIASGGQREHRYEKLLKALREKGLNPENFGFYLEAFKYGMPPHGGFGLGVERLLMRMLN
LENVREAILFVRDRTRLVP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI45439306, Length=327, Percent_Identity=37.3088685015291, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI4758762, Length=328, Percent_Identity=30.1829268292683, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI226491502, Length=442, Percent_Identity=27.6018099547511, Blast_Score=149, Evalue=5e-36,
Organism=Escherichia coli, GI1787161, Length=441, Percent_Identity=26.530612244898, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1790571, Length=370, Percent_Identity=25.6756756756757, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789256, Length=370, Percent_Identity=25.4054054054054, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788173, Length=300, Percent_Identity=26, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082379, Length=334, Percent_Identity=24.251497005988, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17551876, Length=328, Percent_Identity=39.3292682926829, Blast_Score=241, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI71984122, Length=339, Percent_Identity=30.9734513274336, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17556372, Length=411, Percent_Identity=26.2773722627737, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71994340, Length=354, Percent_Identity=26.8361581920904, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17535927, Length=354, Percent_Identity=26.8361581920904, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17535925, Length=354, Percent_Identity=26.8361581920904, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323011, Length=332, Percent_Identity=40.6626506024096, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6321807, Length=433, Percent_Identity=29.5612009237875, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6319872, Length=461, Percent_Identity=26.4642082429501, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6320242, Length=418, Percent_Identity=24.6411483253589, Blast_Score=90, Evalue=7e-19,
Organism=Drosophila melanogaster, GI17136276, Length=325, Percent_Identity=39.3846153846154, Blast_Score=238, Evalue=8e-63,
Organism=Drosophila melanogaster, GI19921528, Length=424, Percent_Identity=28.3018867924528, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI19922416, Length=318, Percent_Identity=23.2704402515723, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020780
- InterPro:   IPR004523
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 48363; Mature: 48231

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLKRICGWIIRKKSIGRIIIIEVSSDSVKPSVLVLKEEREPELFKLGMEIDIGTALCFE
CCHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEECCCCCCCEEECCEEECCEEEEEC
GEEAPEQRSARGVEYIARKIEVYAKPIEPLPVDTMGKVPALLDTRIKYRWLLVRNPVEKA
CCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCEEEEEEEECCHHHHH
IFIIRNSLLNAAREYFSKNGFIEVQTPKIVAAGAEGGATLFKIQYFENDAYLSQSPQLFK
HHHHHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCCCEEEEEEEECCCCCCCCCHHHHH
QMLMAGFTRIYEITPYFRAEKYNTTRHLNESWGIDVEQGFINSVEDVLQTLENLITYIIE
HHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YVLKNNKEELEILGATLKKPSSPFKRLKFQEAVDILRSEGLEISEAEDLSDYAEKKLGEI
HHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
MTEKGYELYFIVGFPWASTGFYYMRDEDGVYTRKFDLDYRGLEIASGGQREHRYEKLLKA
HHCCCCEEEEEEECCCCCCCEEEEECCCCEEEEEECCCCCCEEECCCCCHHHHHHHHHHH
LREKGLNPENFGFYLEAFKYGMPPHGGFGLGVERLLMRMLNLENVREAILFVRDRTRLVP
HHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GLKRICGWIIRKKSIGRIIIIEVSSDSVKPSVLVLKEEREPELFKLGMEIDIGTALCFE
CHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEECCCCCCCEEECCEEECCEEEEEC
GEEAPEQRSARGVEYIARKIEVYAKPIEPLPVDTMGKVPALLDTRIKYRWLLVRNPVEKA
CCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCEEEEEEEECCHHHHH
IFIIRNSLLNAAREYFSKNGFIEVQTPKIVAAGAEGGATLFKIQYFENDAYLSQSPQLFK
HHHHHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCCCEEEEEEEECCCCCCCCCHHHHH
QMLMAGFTRIYEITPYFRAEKYNTTRHLNESWGIDVEQGFINSVEDVLQTLENLITYIIE
HHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YVLKNNKEELEILGATLKKPSSPFKRLKFQEAVDILRSEGLEISEAEDLSDYAEKKLGEI
HHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
MTEKGYELYFIVGFPWASTGFYYMRDEDGVYTRKFDLDYRGLEIASGGQREHRYEKLLKA
HHCCCCEEEEEEECCCCCCCEEEEECCCCEEEEEECCCCCCEEECCCCCHHHHHHHHHHH
LREKGLNPENFGFYLEAFKYGMPPHGGFGLGVERLLMRMLNLENVREAILFVRDRTRLVP
HHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]