Click here to switch to the map view.

The map label for this gene is pheT [H]

Identifier: 218883502

GI number: 218883502

Start: 177898

End: 179571

Strand: Direct

Name: pheT [H]

Synonym: DKAM_0189

Alternate gene names: 218883502

Gene position: 177898-179571 (Clockwise)

Preceding gene: 218883501

Following gene: 218883508

Centisome position: 13.03

GC content: 45.4

Gene sequence:

>1674_bases
ATGCCGGTTATCAGGGTAAGCATTAGCGATATAGAGGCCCTGCTGGGTAGTCGTGTTACATTGGAGAAATTGTATAAAGG
GTTAACGAAGCTTAAATGCGAGGTTGACAATGTTGAGGGAGATATATTAGAGTACGAGGCAAACCATGACCGCCCCGATC
TCTTTAGTGCAGAAGGTCTTGCAAGGGCTCTGAGGCCCTTCCTAGGACTGGAGGGGAAGCAGTACCATATTGTCAATTCA
AGTATTAAAGGATATAGTGAGGGCATCCCTGGGAGACCCTATATAGCTCTCGCCGTGGTAAGGGATGTGGTTTTGAATGA
GGAGGCCATTGTCCAAATAATGCAATTACAAGAGAAACTGGCCTCTACATATGGCCGGGCGCGTAGGAAGGCGAGTATAG
GTGTATACGATCTCGACAAGATTAAACCACCAATATACTATCGAGCTGTCAACCCCTATGAAACCAAGTATAGACCTTTA
AATGAAAACCGTGAAATGACGCTTAGAGAGGTGCTTAGTGAAACTGAGAAGGGGAGGTTGTATGGGTATATAATTGAGTC
CATGGAGAGGTACCCAGTGCTAATGGACTCTGAGGGGACGATACTCAGTCTAGCACCTATACTTAACTCCGATGATAACA
AGGTCACCACTAATACACGCAACATATTGATTGATTCAACAGGTTTAAACCCAGAGATCGTCGTAGACATGGTTACTATA
ATGGCTGTAAACATCGCCGAGAGATCCAAGAACAGGATAATCGAAGTTGTTGGAGTAGAGCATCCCAGCGGATTAATCAT
TGAGGCACCTAGGAGAACGGGCAACTTTATCAGTGTTAATTTAAGTGATGTCAGTAGACTCATAGGCGTCGACATCAGTG
TAGAAGACGCTGTAAGGTATCTCTCACTCCATTATTATACAGTGAGAAGCATTGAGGGAAGAGTCCTCACAGTGGAAGCA
CCTATCTACAGGCTTGATGCTAGAAGCTGGGTTGATGTCGCAGAAGATATAGCTATATCCCATGGCTACGATGCTATAGG
AAGGCAGGCAGACTCCCTACCCTACTCAACCAGCATTGGAAAGATACATCCATTAGAGTATATATCGAGGAGGGTCAGGG
ATATACTAGTAGGACTCGGATTCATCGAGATAGCAAACTACATGATGACTAGTATCGAGTCCCAGACTACATTAATGGGG
TATAACCAGGGGATCTTCGTTGTCGAGAACCCTAAGTCAGATAGGTATACAGCTATAAGGTCATGGCTGACGCCCGGCAT
AATAGAGGCGATAATTGAGAACAAGGGTAAGAGGGCCAGGCTTGCAGTGTTTGAAATCGGGGATGTGGTGCTCCCCGACG
AGTCAATGGAGACGAATGCACGTGTGGAGAGGAGGATAGGTATCGCTGTTTCCCATGAGAAGGCCACGTTAACCGATGGC
CTCGCAGTGGTTAGAACACTCTTCAAAGAGCTAGGTGTAACATTATCCTTTGAAAAAACCATGGTCCCCGGCTTACTCCC
CGAGAGGACAGCTGCTATCAATGTAAACGGGGAACAGATAGGCTTTGTCGGGGAAGTGAAGCCTGAGATACTGTATAAGC
TGGGATTAATGAATCCTGTCATCGTATCTGAAATAAACCTGAATAAAATTATTAAATTACTCGCAACCAATTGA

Upstream 100 bases:

>100_bases
TATTCCAGGTTGAAAACCTGATTAAATGGAGCAGTGAGGAAGAAACATTAATCAATAACGCCTGGGAATTAATTAGAAAT
AAATTATTGAAGTGATAGAT

Downstream 100 bases:

>100_bases
ATATTTTCGAGGATGAGGCTTGGAAGGGATGACTCGAAAGGGGATGAATGCACGGGCCTAGCTGAATCCTCTCCTAGATA
ATAAGGCTACAAGTATTATT

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MPVIRVSISDIEALLGSRVTLEKLYKGLTKLKCEVDNVEGDILEYEANHDRPDLFSAEGLARALRPFLGLEGKQYHIVNS
SIKGYSEGIPGRPYIALAVVRDVVLNEEAIVQIMQLQEKLASTYGRARRKASIGVYDLDKIKPPIYYRAVNPYETKYRPL
NENREMTLREVLSETEKGRLYGYIIESMERYPVLMDSEGTILSLAPILNSDDNKVTTNTRNILIDSTGLNPEIVVDMVTI
MAVNIAERSKNRIIEVVGVEHPSGLIIEAPRRTGNFISVNLSDVSRLIGVDISVEDAVRYLSLHYYTVRSIEGRVLTVEA
PIYRLDARSWVDVAEDIAISHGYDAIGRQADSLPYSTSIGKIHPLEYISRRVRDILVGLGFIEIANYMMTSIESQTTLMG
YNQGIFVVENPKSDRYTAIRSWLTPGIIEAIIENKGKRARLAVFEIGDVVLPDESMETNARVERRIGIAVSHEKATLTDG
LAVVRTLFKELGVTLSFEKTMVPGLLPERTAAINVNGEQIGFVGEVKPEILYKLGLMNPVIVSEINLNKIIKLLATN

Sequences:

>Translated_557_residues
MPVIRVSISDIEALLGSRVTLEKLYKGLTKLKCEVDNVEGDILEYEANHDRPDLFSAEGLARALRPFLGLEGKQYHIVNS
SIKGYSEGIPGRPYIALAVVRDVVLNEEAIVQIMQLQEKLASTYGRARRKASIGVYDLDKIKPPIYYRAVNPYETKYRPL
NENREMTLREVLSETEKGRLYGYIIESMERYPVLMDSEGTILSLAPILNSDDNKVTTNTRNILIDSTGLNPEIVVDMVTI
MAVNIAERSKNRIIEVVGVEHPSGLIIEAPRRTGNFISVNLSDVSRLIGVDISVEDAVRYLSLHYYTVRSIEGRVLTVEA
PIYRLDARSWVDVAEDIAISHGYDAIGRQADSLPYSTSIGKIHPLEYISRRVRDILVGLGFIEIANYMMTSIESQTTLMG
YNQGIFVVENPKSDRYTAIRSWLTPGIIEAIIENKGKRARLAVFEIGDVVLPDESMETNARVERRIGIAVSHEKATLTDG
LAVVRTLFKELGVTLSFEKTMVPGLLPERTAAINVNGEQIGFVGEVKPEILYKLGLMNPVIVSEINLNKIIKLLATN
>Mature_556_residues
PVIRVSISDIEALLGSRVTLEKLYKGLTKLKCEVDNVEGDILEYEANHDRPDLFSAEGLARALRPFLGLEGKQYHIVNSS
IKGYSEGIPGRPYIALAVVRDVVLNEEAIVQIMQLQEKLASTYGRARRKASIGVYDLDKIKPPIYYRAVNPYETKYRPLN
ENREMTLREVLSETEKGRLYGYIIESMERYPVLMDSEGTILSLAPILNSDDNKVTTNTRNILIDSTGLNPEIVVDMVTIM
AVNIAERSKNRIIEVVGVEHPSGLIIEAPRRTGNFISVNLSDVSRLIGVDISVEDAVRYLSLHYYTVRSIEGRVLTVEAP
IYRLDARSWVDVAEDIAISHGYDAIGRQADSLPYSTSIGKIHPLEYISRRVRDILVGLGFIEIANYMMTSIESQTTLMGY
NQGIFVVENPKSDRYTAIRSWLTPGIIEAIIENKGKRARLAVFEIGDVVLPDESMETNARVERRIGIAVSHEKATLTDGL
AVVRTLFKELGVTLSFEKTMVPGLLPERTAAINVNGEQIGFVGEVKPEILYKLGLMNPVIVSEINLNKIIKLLATN

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B5 domain [H]

Homologues:

Organism=Homo sapiens, GI124028525, Length=535, Percent_Identity=27.4766355140187, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI24308207, Length=176, Percent_Identity=27.8409090909091, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788006, Length=328, Percent_Identity=25.609756097561, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17533403, Length=531, Percent_Identity=26.3653483992467, Blast_Score=186, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6323089, Length=600, Percent_Identity=28.3333333333333, Blast_Score=188, Evalue=2e-48,
Organism=Drosophila melanogaster, GI21355211, Length=529, Percent_Identity=29.1115311909263, Blast_Score=211, Evalue=1e-54,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR004531
- InterPro:   IPR022918
- InterPro:   IPR002319 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 62182; Mature: 62050

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVIRVSISDIEALLGSRVTLEKLYKGLTKLKCEVDNVEGDILEYEANHDRPDLFSAEGL
CCEEEEEHHHHHHHHCCCCCHHHHHHHHHHEEEEECCCCCCEEEEECCCCCCCCCCHHHH
ARALRPFLGLEGKQYHIVNSSIKGYSEGIPGRPYIALAVVRDVVLNEEAIVQIMQLQEKL
HHHHHHHHCCCCCEEEEECCCCCCHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHH
ASTYGRARRKASIGVYDLDKIKPPIYYRAVNPYETKYRPLNENREMTLREVLSETEKGRL
HHHHHHHHHHCCCCEEEHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCE
YGYIIESMERYPVLMDSEGTILSLAPILNSDDNKVTTNTRNILIDSTGLNPEIVVDMVTI
EEHHHHHHHHCCEEECCCCCEEEEEECCCCCCCEEEECCCEEEEECCCCCHHHHHHHHHH
MAVNIAERSKNRIIEVVGVEHPSGLIIEAPRRTGNFISVNLSDVSRLIGVDISVEDAVRY
HHHHHHHHCCCCEEEEEEECCCCCCEEECCCCCCCEEEEEHHHHHHHHCCCCCHHHHHHH
LSLHYYTVRSIEGRVLTVEAPIYRLDARSWVDVAEDIAISHGYDAIGRQADSLPYSTSIG
HHEEEEEEEECCCEEEEEECCEEEECCHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCC
KIHPLEYISRRVRDILVGLGFIEIANYMMTSIESQTTLMGYNQGIFVVENPKSDRYTAIR
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCHHHHHH
SWLTPGIIEAIIENKGKRARLAVFEIGDVVLPDESMETNARVERRIGIAVSHEKATLTDG
HHHCHHHHHHHHHCCCCEEEEEEEEECCEECCCCCCCCCCHHHHEEEEEEECCCHHHHHH
LAVVRTLFKELGVTLSFEKTMVPGLLPERTAAINVNGEQIGFVGEVKPEILYKLGLMNPV
HHHHHHHHHHCCCEEEEHHHCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHCCCCCC
IVSEINLNKIIKLLATN
EEEECCHHHHHHHHCCC
>Mature Secondary Structure 
PVIRVSISDIEALLGSRVTLEKLYKGLTKLKCEVDNVEGDILEYEANHDRPDLFSAEGL
CEEEEEHHHHHHHHCCCCCHHHHHHHHHHEEEEECCCCCCEEEEECCCCCCCCCCHHHH
ARALRPFLGLEGKQYHIVNSSIKGYSEGIPGRPYIALAVVRDVVLNEEAIVQIMQLQEKL
HHHHHHHHCCCCCEEEEECCCCCCHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHH
ASTYGRARRKASIGVYDLDKIKPPIYYRAVNPYETKYRPLNENREMTLREVLSETEKGRL
HHHHHHHHHHCCCCEEEHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCE
YGYIIESMERYPVLMDSEGTILSLAPILNSDDNKVTTNTRNILIDSTGLNPEIVVDMVTI
EEHHHHHHHHCCEEECCCCCEEEEEECCCCCCCEEEECCCEEEEECCCCCHHHHHHHHHH
MAVNIAERSKNRIIEVVGVEHPSGLIIEAPRRTGNFISVNLSDVSRLIGVDISVEDAVRY
HHHHHHHHCCCCEEEEEEECCCCCCEEECCCCCCCEEEEEHHHHHHHHCCCCCHHHHHHH
LSLHYYTVRSIEGRVLTVEAPIYRLDARSWVDVAEDIAISHGYDAIGRQADSLPYSTSIG
HHEEEEEEEECCCEEEEEECCEEEECCHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCC
KIHPLEYISRRVRDILVGLGFIEIANYMMTSIESQTTLMGYNQGIFVVENPKSDRYTAIR
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCHHHHHH
SWLTPGIIEAIIENKGKRARLAVFEIGDVVLPDESMETNARVERRIGIAVSHEKATLTDG
HHHCHHHHHHHHHCCCCEEEEEEEEECCEECCCCCCCCCCHHHHEEEEEEECCCHHHHHH
LAVVRTLFKELGVTLSFEKTMVPGLLPERTAAINVNGEQIGFVGEVKPEILYKLGLMNPV
HHHHHHHHHHCCCEEEEHHHCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHCCCCCC
IVSEINLNKIIKLLATN
EEEECCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA