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The map label for this gene is tfb [H]

Identifier: 218883370

GI number: 218883370

Start: 51112

End: 52071

Strand: Reverse

Name: tfb [H]

Synonym: DKAM_0056

Alternate gene names: 218883370

Gene position: 52071-51112 (Counterclockwise)

Preceding gene: 218883371

Following gene: 218883368

Centisome position: 3.81

GC content: 45.62

Gene sequence:

>960_bases
ATGGGTGTTTTAACAATGTTTATAGATTCCACTGCATCATCTCCTGAGTCATCCATGGATAGTGAGTCAAAGAAGCCTAA
GTGTCCTCAGGACAAGATAGTATATGATACAACACACGGGGAATACGTGTGCCAGGACACCGGCGAGGTAATAGAGGAGA
AAGTGATAGATGAGAGGCCTGAATGGAGGGCTTTTACATCAGAGGAGAGGGGTAGACGCGCGAGGACAGGTGGCCCGGTC
ACAGCTACAGTCCATGACATGGGGTTTGCGACAACGATTGATTACACAGATAGGGATGCAGCGGGTAGAAGACTGACAGA
GAAAAAACATGAGCTGGTTAAGCTAAGGAAGTGGCAGGCAAGAACGAGGATAATAACTAGTGTTGAAAGGAACCTAGCAC
AAGCAATGAACGAGCTGGATCGGTTGTCAGATATATTGAATCTGCCCTCGTACGTTAAGGAAGAGGCGGCTAGAATATAT
CGTAATGCAGTTGAAAAAGGAATAGTTAGAGGCAGAAGTATTGAGAGTATAATAGCCGCTACAATATATCTTGCATGCAG
AGAAATGAAGGTACCACGTTCACTTGATGAGATAACAAGACATACCAGGGTGAACAGGAAGGAGATAGCCAGATGCTATA
GGCTCCTATTAAGGGAGCTACATATAAAAGTTACTACAACCGATCCGGTGGACTACGTCCCTAGGATTGTACATGGATTA
GGGCTTCCCGGACAAGCAGTTAAAATAGCTATCGAGATAATTAATAAGGCGAAGGAACAAGGTGTCACGGGTGGAAAGGA
CCCAGCCGGATTAGCAGCTGCAGCAGTATACATCGCTGCTGAACAGCTGGGAGAGAAGAGAACTCAGAAAGAGATAGCAC
ATGTGGCAGGGGTGACAGAGGTAACTGTAAGGAACAGGTATAAGGAACTCGCGAAAATACTCGGACTAGAGTATGAATAA

Upstream 100 bases:

>100_bases
TCAGCCTTAACTCTTTATAAATGGGTTCAATGAAGGGTTTTTTAAGTCTACCGCAAGATTTATAAAGGTATATTCATAAG
GAATATTTGTATGCACCTTA

Downstream 100 bases:

>100_bases
ACCACAGATATTTCTCAGGCTGCTGTGGATTTATAGATAACTTTAATATCTCTAGAAAACTGAATTATTAAGTGGTGGAT
AACGTAGGGTGGGTTAAAAA

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB 1 [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MGVLTMFIDSTASSPESSMDSESKKPKCPQDKIVYDTTHGEYVCQDTGEVIEEKVIDERPEWRAFTSEERGRRARTGGPV
TATVHDMGFATTIDYTDRDAAGRRLTEKKHELVKLRKWQARTRIITSVERNLAQAMNELDRLSDILNLPSYVKEEAARIY
RNAVEKGIVRGRSIESIIAATIYLACREMKVPRSLDEITRHTRVNRKEIARCYRLLLRELHIKVTTTDPVDYVPRIVHGL
GLPGQAVKIAIEIINKAKEQGVTGGKDPAGLAAAAVYIAAEQLGEKRTQKEIAHVAGVTEVTVRNRYKELAKILGLEYE

Sequences:

>Translated_319_residues
MGVLTMFIDSTASSPESSMDSESKKPKCPQDKIVYDTTHGEYVCQDTGEVIEEKVIDERPEWRAFTSEERGRRARTGGPV
TATVHDMGFATTIDYTDRDAAGRRLTEKKHELVKLRKWQARTRIITSVERNLAQAMNELDRLSDILNLPSYVKEEAARIY
RNAVEKGIVRGRSIESIIAATIYLACREMKVPRSLDEITRHTRVNRKEIARCYRLLLRELHIKVTTTDPVDYVPRIVHGL
GLPGQAVKIAIEIINKAKEQGVTGGKDPAGLAAAAVYIAAEQLGEKRTQKEIAHVAGVTEVTVRNRYKELAKILGLEYE
>Mature_318_residues
GVLTMFIDSTASSPESSMDSESKKPKCPQDKIVYDTTHGEYVCQDTGEVIEEKVIDERPEWRAFTSEERGRRARTGGPVT
ATVHDMGFATTIDYTDRDAAGRRLTEKKHELVKLRKWQARTRIITSVERNLAQAMNELDRLSDILNLPSYVKEEAARIYR
NAVEKGIVRGRSIESIIAATIYLACREMKVPRSLDEITRHTRVNRKEIARCYRLLLRELHIKVTTTDPVDYVPRIVHGLG
LPGQAVKIAIEIINKAKEQGVTGGKDPAGLAAAAVYIAAEQLGEKRTQKEIAHVAGVTEVTVRNRYKELAKILGLEYE

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB) [H]

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TFIIB family [H]

Homologues:

Organism=Homo sapiens, GI4504193, Length=284, Percent_Identity=32.0422535211268, Blast_Score=167, Evalue=9e-42,
Organism=Homo sapiens, GI22035556, Length=286, Percent_Identity=27.2727272727273, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17564892, Length=293, Percent_Identity=31.0580204778157, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17531397, Length=189, Percent_Identity=26.984126984127, Blast_Score=85, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6325343, Length=302, Percent_Identity=26.4900662251656, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321685, Length=190, Percent_Identity=26.8421052631579, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17136758, Length=281, Percent_Identity=32.0284697508897, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI281361961, Length=288, Percent_Identity=25.3472222222222, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24647658, Length=288, Percent_Identity=25.3472222222222, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137 [H]

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon [H]

EC number: NA

Molecular weight: Translated: 35846; Mature: 35714

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS00782 TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVLTMFIDSTASSPESSMDSESKKPKCPQDKIVYDTTHGEYVCQDTGEVIEEKVIDERP
CCEEEEEECCCCCCCCHHCCCCCCCCCCCCCCEEEECCCCCEEECHHHHHHHHHHHCCCC
EWRAFTSEERGRRARTGGPVTATVHDMGFATTIDYTDRDAAGRRLTEKKHELVKLRKWQA
CCCCCCCHHHCCCCCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
RTRIITSVERNLAQAMNELDRLSDILNLPSYVKEEAARIYRNAVEKGIVRGRSIESIIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
TIYLACREMKVPRSLDEITRHTRVNRKEIARCYRLLLRELHIKVTTTDPVDYVPRIVHGL
HHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHC
GLPGQAVKIAIEIINKAKEQGVTGGKDPAGLAAAAVYIAAEQLGEKRTQKEIAHVAGVTE
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
VTVRNRYKELAKILGLEYE
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GVLTMFIDSTASSPESSMDSESKKPKCPQDKIVYDTTHGEYVCQDTGEVIEEKVIDERP
CEEEEEECCCCCCCCHHCCCCCCCCCCCCCCEEEECCCCCEEECHHHHHHHHHHHCCCC
EWRAFTSEERGRRARTGGPVTATVHDMGFATTIDYTDRDAAGRRLTEKKHELVKLRKWQA
CCCCCCCHHHCCCCCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
RTRIITSVERNLAQAMNELDRLSDILNLPSYVKEEAARIYRNAVEKGIVRGRSIESIIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
TIYLACREMKVPRSLDEITRHTRVNRKEIARCYRLLLRELHIKVTTTDPVDYVPRIVHGL
HHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHC
GLPGQAVKIAIEIINKAKEQGVTGGKDPAGLAAAAVYIAAEQLGEKRTQKEIAHVAGVTE
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
VTVRNRYKELAKILGLEYE
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]