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The map label for this gene is pyrG [H]

Identifier: 218883371

GI number: 218883371

Start: 52897

End: 54489

Strand: Reverse

Name: pyrG [H]

Synonym: DKAM_0057

Alternate gene names: 218883371

Gene position: 54489-52897 (Counterclockwise)

Preceding gene: 218883373

Following gene: 218883370

Centisome position: 3.99

GC content: 47.58

Gene sequence:

>1593_bases
ATGGTTAAATACGTCTTTGTAACTGGAGGAGTACTATCCGGGCTTGGAAAAGGCATCGTGGCAGCATCAACAGGTCTACT
CTTAAAGTCTATGGGCTACAGGGTTACAGCTATAAAGATAGACCCCTACTTAAACGTGGATGCTGGAACAATGAACCCCT
ATATGCATGGCGAGGTATTTGTCACCGAGGATGGCGGGGAGACTGATCTAGATATAGGGCACTACGAGAGGTTCCTGGGT
ATAGAGTTATCTAGGAAAAATAATATTACATCGGGACAGATATACTATAGCGTAATATCTAAGGAGAGGAAAGGAGAGTA
TCTGGGGCAATGCGTCCAGATAATACCCCATGTAACCAATGAGATCAAGGAGAGAATACGGGAGGTTACGAAGGAGCAGG
GCGCTGATATAGCAATAGTCGAGATAGGGGGTACCGTGGGAGATATAGAAGGACTGCCGTTCCTAGAAGCAGCTAGACAA
ATGAGGTTTGAGGAGGGGGCCTCGAACACGCTTTACATGCATGTTGCCCTGGCACCTGTGTTATCAAGTGGTGAGATCAA
GACCAAACCTATTCAACACAGTATACAGGAGCTTAGACGTATAGGTATACAGCCAGATATCGTGGTGGCTAGATCCACCA
GACCCCTGGACCCAGAGGCGAGATACAAGATCGCGTTATTCGGCAACATACCCGTCGAAGCCGTCTTCAGCAACCCGGAT
GTGGACCTAGTATATAGGGTTCCACTGGTCTTGCATAGCGAGGGTTTATCAAGATATCTTGCCTCAAGGCTTGGATTAGA
ATATAGGGAACCGGATCTAACCCGGTGGGTTGAGATCGTGGAAAAATATTCAAATGCTTCACACGTAGCTAGGATAGCTA
TGGTTGGCAAATACACTAAGATAAGGGATAGCTATATAAGCATTGTTGAGGCTTTACGGCATGCAGGCATATGGAGCAAC
ACGAGGATTGAGCTCCAGTGGTTTGAAGCCTCAGAGATAGAGGCAGGCAAGGTGTCAGTTGAGGAGATATTGAAGGCTGA
TGGGGCTGTGATACTCCCCGGCTTCGGGCGCCGTGGAAGCGAGGGCAAGATACGTGTCATCAAGGCATTCCGTGAAGCCG
ATAAGCCGCTTCTAGGAATATGCTTCGGTATGCAGTTGATGGTCGTGGAGGCCGCTAGAAACATGGCTGGATTGGAGAAC
GCTAACAGTAGTGAGCTAGATCCTTCCACACCCCACCCTGTAATAGATATCCTCCCGGAGCAAAGGAACCTGAACCAGTT
TGGTGGAACACTGAGGCTTGGCAGTAGAAAAATAGTGATCGAGCCAGGTAGCCTGGCCTGGAAGATCTACGGTGTTAGAG
AAGTGTTTGAGAGGCATAGACACCGCTACGGGTTAAACCAGAAATATATCGATATGCTCGAGAAAACCGGGCTAAGAATA
ACTGGTTGGAGTGAGGAGGGATATGCTGAGATAATAGAGTTAAAAGACAGTAATGTATTCTACTATGGGACGCAAGCCCA
TCCAGAGTTCAAGAGCAACCCATTAAAGCCGAGTCCAGTGTATAAATATTTCGTGGAATCCACAAGCCATTAG

Upstream 100 bases:

>100_bases
TCCAGGATATATACCTAGTCTTAACCTTCTGAGGCACCATCTATCCCCCTGTTAATTAAATATTTTTAAGCCATGTATTT
ATTATGTTGAAGCGGGCACC

Downstream 100 bases:

>100_bases
TCATTAAAAATAAGCCTCCTTAACTATATATAATAATGCCCCGATGAAAACCCGTCGGGAGACTACCCTCTGAGAGATGA
AGAGTCCCCTGGCTACGAGG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MVKYVFVTGGVLSGLGKGIVAASTGLLLKSMGYRVTAIKIDPYLNVDAGTMNPYMHGEVFVTEDGGETDLDIGHYERFLG
IELSRKNNITSGQIYYSVISKERKGEYLGQCVQIIPHVTNEIKERIREVTKEQGADIAIVEIGGTVGDIEGLPFLEAARQ
MRFEEGASNTLYMHVALAPVLSSGEIKTKPIQHSIQELRRIGIQPDIVVARSTRPLDPEARYKIALFGNIPVEAVFSNPD
VDLVYRVPLVLHSEGLSRYLASRLGLEYREPDLTRWVEIVEKYSNASHVARIAMVGKYTKIRDSYISIVEALRHAGIWSN
TRIELQWFEASEIEAGKVSVEEILKADGAVILPGFGRRGSEGKIRVIKAFREADKPLLGICFGMQLMVVEAARNMAGLEN
ANSSELDPSTPHPVIDILPEQRNLNQFGGTLRLGSRKIVIEPGSLAWKIYGVREVFERHRHRYGLNQKYIDMLEKTGLRI
TGWSEEGYAEIIELKDSNVFYYGTQAHPEFKSNPLKPSPVYKYFVESTSH

Sequences:

>Translated_530_residues
MVKYVFVTGGVLSGLGKGIVAASTGLLLKSMGYRVTAIKIDPYLNVDAGTMNPYMHGEVFVTEDGGETDLDIGHYERFLG
IELSRKNNITSGQIYYSVISKERKGEYLGQCVQIIPHVTNEIKERIREVTKEQGADIAIVEIGGTVGDIEGLPFLEAARQ
MRFEEGASNTLYMHVALAPVLSSGEIKTKPIQHSIQELRRIGIQPDIVVARSTRPLDPEARYKIALFGNIPVEAVFSNPD
VDLVYRVPLVLHSEGLSRYLASRLGLEYREPDLTRWVEIVEKYSNASHVARIAMVGKYTKIRDSYISIVEALRHAGIWSN
TRIELQWFEASEIEAGKVSVEEILKADGAVILPGFGRRGSEGKIRVIKAFREADKPLLGICFGMQLMVVEAARNMAGLEN
ANSSELDPSTPHPVIDILPEQRNLNQFGGTLRLGSRKIVIEPGSLAWKIYGVREVFERHRHRYGLNQKYIDMLEKTGLRI
TGWSEEGYAEIIELKDSNVFYYGTQAHPEFKSNPLKPSPVYKYFVESTSH
>Mature_530_residues
MVKYVFVTGGVLSGLGKGIVAASTGLLLKSMGYRVTAIKIDPYLNVDAGTMNPYMHGEVFVTEDGGETDLDIGHYERFLG
IELSRKNNITSGQIYYSVISKERKGEYLGQCVQIIPHVTNEIKERIREVTKEQGADIAIVEIGGTVGDIEGLPFLEAARQ
MRFEEGASNTLYMHVALAPVLSSGEIKTKPIQHSIQELRRIGIQPDIVVARSTRPLDPEARYKIALFGNIPVEAVFSNPD
VDLVYRVPLVLHSEGLSRYLASRLGLEYREPDLTRWVEIVEKYSNASHVARIAMVGKYTKIRDSYISIVEALRHAGIWSN
TRIELQWFEASEIEAGKVSVEEILKADGAVILPGFGRRGSEGKIRVIKAFREADKPLLGICFGMQLMVVEAARNMAGLEN
ANSSELDPSTPHPVIDILPEQRNLNQFGGTLRLGSRKIVIEPGSLAWKIYGVREVFERHRHRYGLNQKYIDMLEKTGLRI
TGWSEEGYAEIIELKDSNVFYYGTQAHPEFKSNPLKPSPVYKYFVESTSH

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=542, Percent_Identity=46.8634686346863, Blast_Score=489, Evalue=1e-138,
Organism=Homo sapiens, GI28559085, Length=544, Percent_Identity=44.1176470588235, Blast_Score=464, Evalue=1e-130,
Organism=Homo sapiens, GI28559083, Length=544, Percent_Identity=44.1176470588235, Blast_Score=464, Evalue=1e-130,
Organism=Homo sapiens, GI221316689, Length=544, Percent_Identity=44.1176470588235, Blast_Score=464, Evalue=1e-130,
Organism=Escherichia coli, GI1789142, Length=540, Percent_Identity=46.6666666666667, Blast_Score=486, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI25148299, Length=598, Percent_Identity=38.4615384615385, Blast_Score=398, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6322563, Length=565, Percent_Identity=44.7787610619469, Blast_Score=454, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6319432, Length=561, Percent_Identity=42.2459893048128, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24664469, Length=542, Percent_Identity=45.7564575645756, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI21357815, Length=486, Percent_Identity=43.4156378600823, Blast_Score=386, Evalue=1e-107,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59259; Mature: 59259

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKYVFVTGGVLSGLGKGIVAASTGLLLKSMGYRVTAIKIDPYLNVDAGTMNPYMHGEVF
CEEEEEECCHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTEDGGETDLDIGHYERFLGIELSRKNNITSGQIYYSVISKERKGEYLGQCVQIIPHVTN
EECCCCCCCCCHHHHHHHHEEEEECCCCCCCCEEEEEEHHCHHCHHHHHHHHHHHHHHHH
EIKERIREVTKEQGADIAIVEIGGTVGDIEGLPFLEAARQMRFEEGASNTLYMHVALAPV
HHHHHHHHHHHHCCCCEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEHH
LSSGEIKTKPIQHSIQELRRIGIQPDIVVARSTRPLDPEARYKIALFGNIPVEAVFSNPD
HCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEECCCEEEEECCCC
VDLVYRVPLVLHSEGLSRYLASRLGLEYREPDLTRWVEIVEKYSNASHVARIAMVGKYTK
EEEEEECCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCHHH
IRDSYISIVEALRHAGIWSNTRIELQWFEASEIEAGKVSVEEILKADGAVILPGFGRRGS
HHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHCCCCEEECCCCCCCC
EGKIRVIKAFREADKPLLGICFGMQLMVVEAARNMAGLENANSSELDPSTPHPVIDILPE
CCCEEHEEHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCC
QRNLNQFGGTLRLGSRKIVIEPGSLAWKIYGVREVFERHRHRYGLNQKYIDMLEKTGLRI
CCCHHHCCCEEEECCEEEEEECCCEEEEEECHHHHHHHHHHHCCCCHHHHHHHHHCCCEE
TGWSEEGYAEIIELKDSNVFYYGTQAHPEFKSNPLKPSPVYKYFVESTSH
ECCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MVKYVFVTGGVLSGLGKGIVAASTGLLLKSMGYRVTAIKIDPYLNVDAGTMNPYMHGEVF
CEEEEEECCHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTEDGGETDLDIGHYERFLGIELSRKNNITSGQIYYSVISKERKGEYLGQCVQIIPHVTN
EECCCCCCCCCHHHHHHHHEEEEECCCCCCCCEEEEEEHHCHHCHHHHHHHHHHHHHHHH
EIKERIREVTKEQGADIAIVEIGGTVGDIEGLPFLEAARQMRFEEGASNTLYMHVALAPV
HHHHHHHHHHHHCCCCEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEHH
LSSGEIKTKPIQHSIQELRRIGIQPDIVVARSTRPLDPEARYKIALFGNIPVEAVFSNPD
HCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEECCCEEEEECCCC
VDLVYRVPLVLHSEGLSRYLASRLGLEYREPDLTRWVEIVEKYSNASHVARIAMVGKYTK
EEEEEECCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCHHH
IRDSYISIVEALRHAGIWSNTRIELQWFEASEIEAGKVSVEEILKADGAVILPGFGRRGS
HHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHCCCCEEECCCCCCCC
EGKIRVIKAFREADKPLLGICFGMQLMVVEAARNMAGLENANSSELDPSTPHPVIDILPE
CCCEEHEEHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCC
QRNLNQFGGTLRLGSRKIVIEPGSLAWKIYGVREVFERHRHRYGLNQKYIDMLEKTGLRI
CCCHHHCCCEEEECCEEEEEECCCEEEEEECHHHHHHHHHHHCCCCHHHHHHHHHCCCEE
TGWSEEGYAEIIELKDSNVFYYGTQAHPEFKSNPLKPSPVYKYFVESTSH
ECCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]