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The map label for this gene is 218883363

Identifier: 218883363

GI number: 218883363

Start: 45058

End: 46692

Strand: Direct

Name: 218883363

Synonym: DKAM_0049

Alternate gene names: NA

Gene position: 45058-46692 (Clockwise)

Preceding gene: 218883359

Following gene: 218883367

Centisome position: 3.3

GC content: 46.12

Gene sequence:

>1635_bases
ATGCTCTATATCCAAAAATATTATCTAGGCGAGGCTGGAAAAATGAGGATGCTGGTTATCGATGTACTTGCCAGGTCCAC
GGGTAAAAGGTACTCAACATACGATGTCGTGGGTGCGGGTCCACGAGTGGTAGCTGGCATGCTTTCTGAAAAATACGACA
CATTGCTCATGCCATACGAAAAAGCCATGGGTAGTAAGGACGTTGTCAGAAGTGTTACCCACATCTTTCTATCAGCGATG
AGTAGTGATAAGGGAGCTCTGGGTAGAGTCGTAGGGTATATTAGAAGAGTTAATCCCTCAGCAGTAATTACCGTGGGAGG
GCCGGTTGGCTTTGAATACGGCGAGTTATTGAAGAGGTTCGACATCGACTACGTTATAGTTGGTGAAGCCGAGATACCTC
TTCAAAGATTCATGGAATGCGTAGTTGAGGATAGGTGTAGTAAACACGAGGTTCCGGCGCTAGGTTTTAGAGATAATGGG
GCAGTCACCTTAACGACTAGGCACATACACACTCCCAGCGATGTTTTATCCTCTATCAAACCCTGGGCACGGATAGATGA
AATGTTCGACTATCCACAGGTTTACAGGGTATATGTGGAAGCCGTGCGGGGATGCAGTAATTTCATGCGCCCTATGGTGG
GGAGCATAGGTTGTATATTCTGCGGAAACTGTAGAAGCAGTAAGAATGAGGAACGAGTTAACTGCCCAGCCAATATCCCG
CCTGGTTGCGGTTTTTGCAGTGTTCCCTCAATGTTTGGTCCAGCTAGGTCGAGGAGTGTTGATAGTATAGTGAAAGAAGT
AGAGGAGTTAATCGAGCATGGTGCACGTAGGATAGTGTTAAGCGCCCCGGATATCCTAGACTATGGGAGGGATTTACTCG
TAAATGGGCCTTTAACTAATCCGTGTAACCCACCAGCGAACACCGAAGCTATCGAGGAGTTGCTATCACGTCTCAACGCG
TTGAAACCAGTGTCCCAGGGTAGAGTTGTAGTCATGATTGAGAATATCAAGGCATGCCTGGTCAACGATGAAGTAGCTAA
AATACTGGGAAAATACCTGCCTGGGACAACAGTACATATAGGGCTTGAAACCGGTAGTGATACATATAATGACAAGGTAT
TAGGGAAACCCATAAAAGTAGCTGACGTAGTGAACGCGGTGAGGCTCTTAAAGAAGAACTCGCTTAGACCCTACGTATAC
CTGATGCATAGCCTGCCCTTCGCCAGCAGGAAGATATATGAGGAGACGCTCAAAACCGTTAACGAACTAGGTGAGGCAGG
TGTTGAAAAAATAACCCTCTACAAGTATACTCCCCTCCCATATACGGCCTTTGAAAAAATACCATCCGAAGAACGTGGCG
TGGAGGAATGGATAGCCAGGTTGAAAGCCGTCGTAAGAAGGTATAACACTATGCAGAAAAAGGAAATCCTGGGTAAGCAT
GTAGAGGCCTTCATAATTGAAAGTAATGGCAGGGTCTATGGATATCCAGTTAAACATGGACCAGTGATATTTCTAGGTCG
GATAGCCAGGAGGGGTCTGAATGGTTGTTTAGTGATAGTTGAGGTGACTGGAACACGGTATAGATATGTGAAGGGTAGGA
TCGTGAAGATTAAAGAGTGTCCTCCAGCTTCTTGA

Upstream 100 bases:

>100_bases
ATTAGTATAAAGGGTATTTAAGTAATTTGGGAGTCAATGACATCGGTATGTATATAGATCAAGCAGTGGGGAACTTTTCC
GAGGATAGTGCACCGCTATA

Downstream 100 bases:

>100_bases
ACCCGGCTAAAAGCCTCTGAAGCTCGCTTAATACCTCTGGCATCCCCTGGTAGACTGGTAAGGCTTCCTCTAGGAGATCC
ACAATGTATCTTATACTGAT

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MLYIQKYYLGEAGKMRMLVIDVLARSTGKRYSTYDVVGAGPRVVAGMLSEKYDTLLMPYEKAMGSKDVVRSVTHIFLSAM
SSDKGALGRVVGYIRRVNPSAVITVGGPVGFEYGELLKRFDIDYVIVGEAEIPLQRFMECVVEDRCSKHEVPALGFRDNG
AVTLTTRHIHTPSDVLSSIKPWARIDEMFDYPQVYRVYVEAVRGCSNFMRPMVGSIGCIFCGNCRSSKNEERVNCPANIP
PGCGFCSVPSMFGPARSRSVDSIVKEVEELIEHGARRIVLSAPDILDYGRDLLVNGPLTNPCNPPANTEAIEELLSRLNA
LKPVSQGRVVVMIENIKACLVNDEVAKILGKYLPGTTVHIGLETGSDTYNDKVLGKPIKVADVVNAVRLLKKNSLRPYVY
LMHSLPFASRKIYEETLKTVNELGEAGVEKITLYKYTPLPYTAFEKIPSEERGVEEWIARLKAVVRRYNTMQKKEILGKH
VEAFIIESNGRVYGYPVKHGPVIFLGRIARRGLNGCLVIVEVTGTRYRYVKGRIVKIKECPPAS

Sequences:

>Translated_544_residues
MLYIQKYYLGEAGKMRMLVIDVLARSTGKRYSTYDVVGAGPRVVAGMLSEKYDTLLMPYEKAMGSKDVVRSVTHIFLSAM
SSDKGALGRVVGYIRRVNPSAVITVGGPVGFEYGELLKRFDIDYVIVGEAEIPLQRFMECVVEDRCSKHEVPALGFRDNG
AVTLTTRHIHTPSDVLSSIKPWARIDEMFDYPQVYRVYVEAVRGCSNFMRPMVGSIGCIFCGNCRSSKNEERVNCPANIP
PGCGFCSVPSMFGPARSRSVDSIVKEVEELIEHGARRIVLSAPDILDYGRDLLVNGPLTNPCNPPANTEAIEELLSRLNA
LKPVSQGRVVVMIENIKACLVNDEVAKILGKYLPGTTVHIGLETGSDTYNDKVLGKPIKVADVVNAVRLLKKNSLRPYVY
LMHSLPFASRKIYEETLKTVNELGEAGVEKITLYKYTPLPYTAFEKIPSEERGVEEWIARLKAVVRRYNTMQKKEILGKH
VEAFIIESNGRVYGYPVKHGPVIFLGRIARRGLNGCLVIVEVTGTRYRYVKGRIVKIKECPPAS
>Mature_544_residues
MLYIQKYYLGEAGKMRMLVIDVLARSTGKRYSTYDVVGAGPRVVAGMLSEKYDTLLMPYEKAMGSKDVVRSVTHIFLSAM
SSDKGALGRVVGYIRRVNPSAVITVGGPVGFEYGELLKRFDIDYVIVGEAEIPLQRFMECVVEDRCSKHEVPALGFRDNG
AVTLTTRHIHTPSDVLSSIKPWARIDEMFDYPQVYRVYVEAVRGCSNFMRPMVGSIGCIFCGNCRSSKNEERVNCPANIP
PGCGFCSVPSMFGPARSRSVDSIVKEVEELIEHGARRIVLSAPDILDYGRDLLVNGPLTNPCNPPANTEAIEELLSRLNA
LKPVSQGRVVVMIENIKACLVNDEVAKILGKYLPGTTVHIGLETGSDTYNDKVLGKPIKVADVVNAVRLLKKNSLRPYVY
LMHSLPFASRKIYEETLKTVNELGEAGVEKITLYKYTPLPYTAFEKIPSEERGVEEWIARLKAVVRRYNTMQKKEILGKH
VEAFIIESNGRVYGYPVKHGPVIFLGRIARRGLNGCLVIVEVTGTRYRYVKGRIVKIKECPPAS

Specific function: Unknown

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 60541; Mature: 60541

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYIQKYYLGEAGKMRMLVIDVLARSTGKRYSTYDVVGAGPRVVAGMLSEKYDTLLMPYE
CEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHH
KAMGSKDVVRSVTHIFLSAMSSDKGALGRVVGYIRRVNPSAVITVGGPVGFEYGELLKRF
HHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHC
DIDYVIVGEAEIPLQRFMECVVEDRCSKHEVPALGFRDNGAVTLTTRHIHTPSDVLSSIK
CCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHCC
PWARIDEMFDYPQVYRVYVEAVRGCSNFMRPMVGSIGCIFCGNCRSSKNEERVNCPANIP
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCC
PGCGFCSVPSMFGPARSRSVDSIVKEVEELIEHGARRIVLSAPDILDYGRDLLVNGPLTN
CCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCEEEECCCCC
PCNPPANTEAIEELLSRLNALKPVSQGRVVVMIENIKACLVNDEVAKILGKYLPGTTVHI
CCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHEECHHHHHHHHHHCCCCEEEE
GLETGSDTYNDKVLGKPIKVADVVNAVRLLKKNSLRPYVYLMHSLPFASRKIYEETLKTV
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHH
NELGEAGVEKITLYKYTPLPYTAFEKIPSEERGVEEWIARLKAVVRRYNTMQKKEILGKH
HHHHHCCCCEEEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VEAFIIESNGRVYGYPVKHGPVIFLGRIARRGLNGCLVIVEVTGTRYRYVKGRIVKIKEC
EEEEEEECCCEEEEEECCCCCEEEEEHHHHCCCCCEEEEEEECCCEEEEECCEEEEEECC
PPAS
CCCC
>Mature Secondary Structure
MLYIQKYYLGEAGKMRMLVIDVLARSTGKRYSTYDVVGAGPRVVAGMLSEKYDTLLMPYE
CEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHH
KAMGSKDVVRSVTHIFLSAMSSDKGALGRVVGYIRRVNPSAVITVGGPVGFEYGELLKRF
HHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHC
DIDYVIVGEAEIPLQRFMECVVEDRCSKHEVPALGFRDNGAVTLTTRHIHTPSDVLSSIK
CCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHCC
PWARIDEMFDYPQVYRVYVEAVRGCSNFMRPMVGSIGCIFCGNCRSSKNEERVNCPANIP
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCC
PGCGFCSVPSMFGPARSRSVDSIVKEVEELIEHGARRIVLSAPDILDYGRDLLVNGPLTN
CCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCEEEECCCCC
PCNPPANTEAIEELLSRLNALKPVSQGRVVVMIENIKACLVNDEVAKILGKYLPGTTVHI
CCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHEECHHHHHHHHHHCCCCEEEE
GLETGSDTYNDKVLGKPIKVADVVNAVRLLKKNSLRPYVYLMHSLPFASRKIYEETLKTV
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHH
NELGEAGVEKITLYKYTPLPYTAFEKIPSEERGVEEWIARLKAVVRRYNTMQKKEILGKH
HHHHHCCCCEEEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VEAFIIESNGRVYGYPVKHGPVIFLGRIARRGLNGCLVIVEVTGTRYRYVKGRIVKIKEC
EEEEEEECCCEEEEEECCCCCEEEEEHHHHCCCCCEEEEEEECCCEEEEECCEEEEEECC
PPAS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]