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The map label for this gene is speB [C]

Identifier: 218883367

GI number: 218883367

Start: 49298

End: 50176

Strand: Direct

Name: speB [C]

Synonym: DKAM_0053

Alternate gene names: 218883367

Gene position: 49298-50176 (Clockwise)

Preceding gene: 218883363

Following gene: 218883369

Centisome position: 3.61

GC content: 45.62

Gene sequence:

>879_bases
GTGGTGGTGAACATGAATTGGAGGCTACTACTACAGGGATCAGTGAAGTTTGCCTGTATGGAGTCCAGCAGTAATATAGG
GGTATTAATCGGTGTGCCCATGGATTACACATCAACATATAAGCCTGGTTCAAGATTCGCACCGGACTATATAAGGAACG
CATCATGTAACATTGAGTTCTATAGTTTGTCAACTAGGAGAATCATGGAGAGCGAGGGTTTCATCGACTTGGGAAACATT
ATCCTGCCACCCGGAGATGTAGAGAAATCCCTATCAATAATACAAAGTGTTGCCAAGGGGGCTTGGAGGGAGTACCAGGG
GATACCCCTCGGTTTCTTAGGAGGGGAACACTTAATCACCTACCCAATTGTAAGCGGGCTAATGGACGGCATTGACACCC
TAGTGGTTTTTGATGCGCATCTCGATCTAAGGGATGAATACCTCGGCTCAAGATTGAATCACGCATCATTCCTTAGAAGA
CTCGTTGAGAAAGGTGTGAGAGTTATACATATAGGCTCCAGGGCCTACAGTGGTGAGGAGTTGGAGTACTTGAAAACCTT
GGAAAATATCAAGGTATACTCAGTTCTAGACGTTCTTGAAAAAACAGTGGAAGCAGTGGAGTTAGGGAGGGTATATATAA
GCATAGATATGGACGTTATTGATCCATCATATGCGCCTGGCGTGGGGAACCCGGAGCCCTTCGGGTTAACCCCCCTACAA
TTACTTGGACTATTAAAGAAAATAGTGGGGTCGTCGAGCCGCCTGGTAGGATTTGATATAGTTGAAGTAAACCCGCTGGT
CGATGTAAACGATGTTACAAGTATACTGGCTGGTAAACTAGTATTTGAGCTAGCTGCATTAGCCAGGGGTGGGAGATAG

Upstream 100 bases:

>100_bases
CTAATTCCATTATGTTACCATATATGTCTTCTCCATTACTCAACTCGATCCCCTTGTACTTGGATTTTAAATAACGCGTT
CACCTTAAATATTCGTTTAA

Downstream 100 bases:

>100_bases
ACCTATTGGGGTCTACTAGGGAGTTCAGGCCTCATATAGGTTTATATAAGCTTGTTTCATCCCCTCGTTTTTGTTCCCCC
TGTATCCTGCTTGGGCTTGC

Product: Agmatinase

Products: putrescine; urea

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MVVNMNWRLLLQGSVKFACMESSSNIGVLIGVPMDYTSTYKPGSRFAPDYIRNASCNIEFYSLSTRRIMESEGFIDLGNI
ILPPGDVEKSLSIIQSVAKGAWREYQGIPLGFLGGEHLITYPIVSGLMDGIDTLVVFDAHLDLRDEYLGSRLNHASFLRR
LVEKGVRVIHIGSRAYSGEELEYLKTLENIKVYSVLDVLEKTVEAVELGRVYISIDMDVIDPSYAPGVGNPEPFGLTPLQ
LLGLLKKIVGSSSRLVGFDIVEVNPLVDVNDVTSILAGKLVFELAALARGGR

Sequences:

>Translated_292_residues
MVVNMNWRLLLQGSVKFACMESSSNIGVLIGVPMDYTSTYKPGSRFAPDYIRNASCNIEFYSLSTRRIMESEGFIDLGNI
ILPPGDVEKSLSIIQSVAKGAWREYQGIPLGFLGGEHLITYPIVSGLMDGIDTLVVFDAHLDLRDEYLGSRLNHASFLRR
LVEKGVRVIHIGSRAYSGEELEYLKTLENIKVYSVLDVLEKTVEAVELGRVYISIDMDVIDPSYAPGVGNPEPFGLTPLQ
LLGLLKKIVGSSSRLVGFDIVEVNPLVDVNDVTSILAGKLVFELAALARGGR
>Mature_292_residues
MVVNMNWRLLLQGSVKFACMESSSNIGVLIGVPMDYTSTYKPGSRFAPDYIRNASCNIEFYSLSTRRIMESEGFIDLGNI
ILPPGDVEKSLSIIQSVAKGAWREYQGIPLGFLGGEHLITYPIVSGLMDGIDTLVVFDAHLDLRDEYLGSRLNHASFLRR
LVEKGVRVIHIGSRAYSGEELEYLKTLENIKVYSVLDVLEKTVEAVELGRVYISIDMDVIDPSYAPGVGNPEPFGLTPLQ
LLGLLKKIVGSSSRLVGFDIVEVNPLVDVNDVTSILAGKLVFELAALARGGR

Specific function: Catalyzes The Formation Of Putrescine From Agmatine. [C]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family [H]

Homologues:

Organism=Homo sapiens, GI37537722, Length=284, Percent_Identity=34.5070422535211, Blast_Score=132, Evalue=4e-31,
Organism=Escherichia coli, GI1789306, Length=259, Percent_Identity=27.027027027027, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855 [H]

Pfam domain/function: PF00491 Arginase [H]

EC number: 3.5.3.11

Molecular weight: Translated: 32072; Mature: 32072

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00147 ARGINASE_1 ; PS00148 ARGINASE_2 ; PS01053 ARGINASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVNMNWRLLLQGSVKFACMESSSNIGVLIGVPMDYTSTYKPGSRFAPDYIRNASCNIEF
CEECCCEEEEEECCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCEEE
YSLSTRRIMESEGFIDLGNIILPPGDVEKSLSIIQSVAKGAWREYQGIPLGFLGGEHLIT
EECCHHHHHHCCCCEEECCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEH
YPIVSGLMDGIDTLVVFDAHLDLRDEYLGSRLNHASFLRRLVEKGVRVIHIGSRAYSGEE
HHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCHH
LEYLKTLENIKVYSVLDVLEKTVEAVELGRVYISIDMDVIDPSYAPGVGNPEPFGLTPLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEEECCCCCCCCCCCCCCCCCHHH
LLGLLKKIVGSSSRLVGFDIVEVNPLVDVNDVTSILAGKLVFELAALARGGR
HHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVVNMNWRLLLQGSVKFACMESSSNIGVLIGVPMDYTSTYKPGSRFAPDYIRNASCNIEF
CEECCCEEEEEECCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCEEE
YSLSTRRIMESEGFIDLGNIILPPGDVEKSLSIIQSVAKGAWREYQGIPLGFLGGEHLIT
EECCHHHHHHCCCCEEECCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEH
YPIVSGLMDGIDTLVVFDAHLDLRDEYLGSRLNHASFLRRLVEKGVRVIHIGSRAYSGEE
HHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCHH
LEYLKTLENIKVYSVLDVLEKTVEAVELGRVYISIDMDVIDPSYAPGVGNPEPFGLTPLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEEECCCCCCCCCCCCCCCCCHHH
LLGLLKKIVGSSSRLVGFDIVEVNPLVDVNDVTSILAGKLVFELAALARGGR
HHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: agmatine; H2O

Specific reaction: agmatine + H2O = putrescine + urea

General reaction: Amidine hydrolysis [C]

Inhibitor: EDTA; EGTA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]