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The map label for this gene is fabI1 [H]

Identifier: 218532547

GI number: 218532547

Start: 4943139

End: 4943966

Strand: Reverse

Name: fabI1 [H]

Synonym: Mchl_4661

Alternate gene names: 218532547

Gene position: 4943966-4943139 (Counterclockwise)

Preceding gene: 218532548

Following gene: 218532543

Centisome position: 85.57

GC content: 65.82

Gene sequence:

>828_bases
ATGTCGGAACAGAAGCCGGGCGGGCTGCTCGCGGGCAAGCGCGGGCTGGTGTTGGGAGTGGCCAACAACCGCTCGATCGC
CTGGGGTATCGCCCGTAGCGCTGCCGCGCACGGGGCGGAATTGGCGTTCACCTACCAGGGCGAGGCGCTGAAGAAGCGCG
TCGAGCCGCTGGCGCGCGAGTTGAACGCGCATGTCGTCGGCCATTGCGACGTCACCGATCCCGCCTCGATCGATTCCGCC
TTCGCCGCGACCGCCGAGGTGTTCCCGGACGGGATCGATTTCGTCGTCCACTGCATCGCCTTCTCCGACAAGGACGAGCT
GACGGGGCGCTATCTCGAGACCTCGGAGGCGAACTTCACCAAGTCGCTGCTGATCTCGTGCTACTCGTTCACGGCGGTGG
CGCAGCGCGCGGAAAAGATCATGCGCAACGGCGGGTCGATGCTGACCCTGACCTATTACGGCGCCGAGAAGTGGATGCCC
CACTACAACGTGATGGGTGTCGCCAAGGCCGCGCTGGAAGCCTCCGTACGCTACCTCGCCGCCGATCTCGGGCCGAAGCA
GATCCGCGTCAACGCGATCTCGGCCGGGCCGATCAAGACGCTGGCGGCCTCCGGCATCGGCGACTTCCGCTACATTCTCA
AGTGGAACGAGTACAACGCGCCGATGCGCCGGACCGTGACCATTGAGGAGGTCGGCGAGACGGCAGCCTACCTGTTCTCC
GACATGTCCCGCGGCATGACCGGCGAGATCCTCCACGTCGATGCCGGCTACCACGTCGTCGGCATGAAGAACCCCGACGC
GCCGGATATCACCCGCGAGCGGGAATAG

Upstream 100 bases:

>100_bases
CGCCTTGAGGCTTCCCCCTCCGGCAACCCTCGGCTAAGGAAGCCCCCGGCGCAGGGCCGTCCCTCAAGAGGCGCCCGCGC
GGCCGAAGCGAAGGTGACGC

Downstream 100 bases:

>100_bases
GGTTCATCCCTCTTCCCCGAGCCGTCGGCGTCAATCCTCCACGGCGCCGCTCGCGAGCCGGCGGGCGGCGAGCGCCCGCC
AGGATTCGGTGAGGGGCGCG

Product: enoyl-acyl carrier protein reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSEQKPGGLLAGKRGLVLGVANNRSIAWGIARSAAAHGAELAFTYQGEALKKRVEPLARELNAHVVGHCDVTDPASIDSA
FAATAEVFPDGIDFVVHCIAFSDKDELTGRYLETSEANFTKSLLISCYSFTAVAQRAEKIMRNGGSMLTLTYYGAEKWMP
HYNVMGVAKAALEASVRYLAADLGPKQIRVNAISAGPIKTLAASGIGDFRYILKWNEYNAPMRRTVTIEEVGETAAYLFS
DMSRGMTGEILHVDAGYHVVGMKNPDAPDITRERE

Sequences:

>Translated_275_residues
MSEQKPGGLLAGKRGLVLGVANNRSIAWGIARSAAAHGAELAFTYQGEALKKRVEPLARELNAHVVGHCDVTDPASIDSA
FAATAEVFPDGIDFVVHCIAFSDKDELTGRYLETSEANFTKSLLISCYSFTAVAQRAEKIMRNGGSMLTLTYYGAEKWMP
HYNVMGVAKAALEASVRYLAADLGPKQIRVNAISAGPIKTLAASGIGDFRYILKWNEYNAPMRRTVTIEEVGETAAYLFS
DMSRGMTGEILHVDAGYHVVGMKNPDAPDITRERE
>Mature_274_residues
SEQKPGGLLAGKRGLVLGVANNRSIAWGIARSAAAHGAELAFTYQGEALKKRVEPLARELNAHVVGHCDVTDPASIDSAF
AATAEVFPDGIDFVVHCIAFSDKDELTGRYLETSEANFTKSLLISCYSFTAVAQRAEKIMRNGGSMLTLTYYGAEKWMPH
YNVMGVAKAALEASVRYLAADLGPKQIRVNAISAGPIKTLAASGIGDFRYILKWNEYNAPMRRTVTIEEVGETAAYLFSD
MSRGMTGEILHVDAGYHVVGMKNPDAPDITRERE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=257, Percent_Identity=51.7509727626459, Blast_Score=257, Evalue=5e-70,
Organism=Escherichia coli, GI87082100, Length=265, Percent_Identity=30.9433962264151, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 29823; Mature: 29692

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQKPGGLLAGKRGLVLGVANNRSIAWGIARSAAAHGAELAFTYQGEALKKRVEPLARE
CCCCCCCCEEECCCEEEEEECCCCEEEEHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHH
LNAHVVGHCDVTDPASIDSAFAATAEVFPDGIDFVVHCIAFSDKDELTGRYLETSEANFT
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCCEEECCCCCHH
KSLLISCYSFTAVAQRAEKIMRNGGSMLTLTYYGAEKWMPHYNVMGVAKAALEASVRYLA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHH
ADLGPKQIRVNAISAGPIKTLAASGIGDFRYILKWNEYNAPMRRTVTIEEVGETAAYLFS
HCCCCCEEEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHH
DMSRGMTGEILHVDAGYHVVGMKNPDAPDITRERE
HHHCCCCCEEEEEECCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SEQKPGGLLAGKRGLVLGVANNRSIAWGIARSAAAHGAELAFTYQGEALKKRVEPLARE
CCCCCCCEEECCCEEEEEECCCCEEEEHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHH
LNAHVVGHCDVTDPASIDSAFAATAEVFPDGIDFVVHCIAFSDKDELTGRYLETSEANFT
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCCEEECCCCCHH
KSLLISCYSFTAVAQRAEKIMRNGGSMLTLTYYGAEKWMPHYNVMGVAKAALEASVRYLA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHH
ADLGPKQIRVNAISAGPIKTLAASGIGDFRYILKWNEYNAPMRRTVTIEEVGETAAYLFS
HCCCCCEEEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHH
DMSRGMTGEILHVDAGYHVVGMKNPDAPDITRERE
HHHCCCCCEEEEEECCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]