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The map label for this gene is emrB [H]

Identifier: 218532357

GI number: 218532357

Start: 4729914

End: 4731491

Strand: Reverse

Name: emrB [H]

Synonym: Mchl_4469

Alternate gene names: 218532357

Gene position: 4731491-4729914 (Counterclockwise)

Preceding gene: 218532358

Following gene: 218532356

Centisome position: 81.89

GC content: 66.79

Gene sequence:

>1578_bases
ATGGCCGCCAGCGCGACCCTCGCCGCAAGCCCTGCCGCCGACCCGCCCCTCGACCGCCGCCGCATGGTGGCGTTCCTGTG
CATGGTGTTCGGGATGTTCATGGCGATCCTCGACATCCAGATCGTCTCGGCCTCGCTCAACGAGATCCAGGCCGGCCTCT
CCGCCTCCGGCGACGAGATCCCCTGGGTGCAGACGAGCTACCTCATCGCCGAGGTCATCTCGATCCCGCTCTCGGGCACC
CTGTCGCGGGTGCTCTCGACGCGCTGGATGTTCTCGATCTCGGCGGCCGGCTTCACGCTGATGAGCCTGATGTGCGCCAC
CTCCTCGTCGATCGGCGAGATGATCGTCTGGCGCGCGCTCCAGGGCTTCATCGGCGGCGGCATGATCCCGACCGTGTTCG
CTGCGGCCTTCACGATCTTCCCGCCCTCCAAGCGCTCGATCGTCTCGCCGATGATCGGCCTCGTGGCGACGCTGGCGCCG
ACCATCGGCCCGACCATCGGCGGCTACCTCACCGACCTGTTCTCCTGGCACTGGCTGTTCCTCATCAACATCGTGCCGGG
CATCTTCGTCACGATCTCGACCTTCCTCCTGATTGATTTCGACCGGCCGAACTTCGACCTGCTCAAGTCCTTCGATTGGG
CCGGGCTCGCCTTCATGGCGGGCTTCCTCGGCTGCCTCGAATACGTGCTGGAGGAGGGCCCGAACCACGACTGGCTGCAG
GACGAGGCGGTGTTCGTCTGCGCCCTCGTCTGCGTCGTGTCGGGCTTGGCCTTCTTCGCCCGCGTCTTCACCGCGCGCCA
GCCGATCGTCGACCTGCGCGCCTTCTCGGACCGCAACTTCGCCGCCGGCTGCGTCTTCAGCTTCGTGATGGGCATCGGCC
TCTACGGCCTGACCTACCTCTACCCGGTCTATCTCGCCCGCGTGCGCGGCTACTCGGCGTTGCAGATCGGCGAGACGATG
TTCGTCTCAGGCCTGTGCATGTTCGCCACCGCGCCGATCGCCGGAAAGCTCTCCGCCAAGCTCGATCCGCGCATCATGAT
GGCGATGGGCTTCTCCGGCTTTGCGGTCGGCACCTGGATCGTCACCGGGCTGACCAAGGACTGGGACTTCTGGGAGCTGC
TGTGGCCGCAGGTGCTGCGCGGCTGCTCCCTGATGCTGTGCATGATCCCGATCAACAACATCGCGCTCGGCACCCTGCCG
CCGGAGCGGATGAAGAACGCCTCGGGCCTGTTCAATCTCACGCGCAACCTCGGTGGCGCGGTCGGCCTCGCCCTCATCAA
CACGGTGCTCAACGCCCGCTGGGATCTCCACCTCGCTCGCCTGCACGAGCGCTTCACCTGGGCCAACAGCGCCGCGCTGG
AACGGCTCGATGCGATGCGGCGCCAGTTCGAGGTGTTCGGGGGCGATGCCAACGGTATGGCGCTGAAGGCGCTCAACAAC
ACCGTGCGGATTCAAGGCTTGGTGATGAGCTTCGAGGACGTGTTCCTCGTCCTCACCGTGCTGTTCCTGGCCATGGCCTG
CGGGACGCCGCTGATCCGGCGTCCGCGCGCGGCGGCGCCGGCCGGCGCGGGGCATTGA

Upstream 100 bases:

>100_bases
GCTGCGGTCCCTGGAGCCCTCCTTCGAGGCCCGCTTCGCGGGCACCTCAGGATGAGGTCGCCGGGTGGAACTCACCCCCG
AACCGTCACCGAGGCGCCCC

Downstream 100 bases:

>100_bases
GGGATTTCTTCGGGACGGCGCGCCCGGACGGGTTCGCCGTGCGAAGGAGGGTATTCGGAAGGGCGTCGAAAGGCGCGTAT
GCGGCCGCTCCACGGAATGA

Product: drug resistance transporter EmrB/QacA subfamily

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MAASATLAASPAADPPLDRRRMVAFLCMVFGMFMAILDIQIVSASLNEIQAGLSASGDEIPWVQTSYLIAEVISIPLSGT
LSRVLSTRWMFSISAAGFTLMSLMCATSSSIGEMIVWRALQGFIGGGMIPTVFAAAFTIFPPSKRSIVSPMIGLVATLAP
TIGPTIGGYLTDLFSWHWLFLINIVPGIFVTISTFLLIDFDRPNFDLLKSFDWAGLAFMAGFLGCLEYVLEEGPNHDWLQ
DEAVFVCALVCVVSGLAFFARVFTARQPIVDLRAFSDRNFAAGCVFSFVMGIGLYGLTYLYPVYLARVRGYSALQIGETM
FVSGLCMFATAPIAGKLSAKLDPRIMMAMGFSGFAVGTWIVTGLTKDWDFWELLWPQVLRGCSLMLCMIPINNIALGTLP
PERMKNASGLFNLTRNLGGAVGLALINTVLNARWDLHLARLHERFTWANSAALERLDAMRRQFEVFGGDANGMALKALNN
TVRIQGLVMSFEDVFLVLTVLFLAMACGTPLIRRPRAAAPAGAGH

Sequences:

>Translated_525_residues
MAASATLAASPAADPPLDRRRMVAFLCMVFGMFMAILDIQIVSASLNEIQAGLSASGDEIPWVQTSYLIAEVISIPLSGT
LSRVLSTRWMFSISAAGFTLMSLMCATSSSIGEMIVWRALQGFIGGGMIPTVFAAAFTIFPPSKRSIVSPMIGLVATLAP
TIGPTIGGYLTDLFSWHWLFLINIVPGIFVTISTFLLIDFDRPNFDLLKSFDWAGLAFMAGFLGCLEYVLEEGPNHDWLQ
DEAVFVCALVCVVSGLAFFARVFTARQPIVDLRAFSDRNFAAGCVFSFVMGIGLYGLTYLYPVYLARVRGYSALQIGETM
FVSGLCMFATAPIAGKLSAKLDPRIMMAMGFSGFAVGTWIVTGLTKDWDFWELLWPQVLRGCSLMLCMIPINNIALGTLP
PERMKNASGLFNLTRNLGGAVGLALINTVLNARWDLHLARLHERFTWANSAALERLDAMRRQFEVFGGDANGMALKALNN
TVRIQGLVMSFEDVFLVLTVLFLAMACGTPLIRRPRAAAPAGAGH
>Mature_524_residues
AASATLAASPAADPPLDRRRMVAFLCMVFGMFMAILDIQIVSASLNEIQAGLSASGDEIPWVQTSYLIAEVISIPLSGTL
SRVLSTRWMFSISAAGFTLMSLMCATSSSIGEMIVWRALQGFIGGGMIPTVFAAAFTIFPPSKRSIVSPMIGLVATLAPT
IGPTIGGYLTDLFSWHWLFLINIVPGIFVTISTFLLIDFDRPNFDLLKSFDWAGLAFMAGFLGCLEYVLEEGPNHDWLQD
EAVFVCALVCVVSGLAFFARVFTARQPIVDLRAFSDRNFAAGCVFSFVMGIGLYGLTYLYPVYLARVRGYSALQIGETMF
VSGLCMFATAPIAGKLSAKLDPRIMMAMGFSGFAVGTWIVTGLTKDWDFWELLWPQVLRGCSLMLCMIPINNIALGTLPP
ERMKNASGLFNLTRNLGGAVGLALINTVLNARWDLHLARLHERFTWANSAALERLDAMRRQFEVFGGDANGMALKALNNT
VRIQGLVMSFEDVFLVLTVLFLAMACGTPLIRRPRAAAPAGAGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=497, Percent_Identity=30.5835010060362, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1788710, Length=491, Percent_Identity=28.5132382892057, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1790195, Length=445, Percent_Identity=25.6179775280899, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI87081983, Length=426, Percent_Identity=29.1079812206573, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1788392, Length=413, Percent_Identity=26.8765133171913, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6325455, Length=155, Percent_Identity=30.9677419354839, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57071; Mature: 56940

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASATLAASPAADPPLDRRRMVAFLCMVFGMFMAILDIQIVSASLNEIQAGLSASGDEI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PWVQTSYLIAEVISIPLSGTLSRVLSTRWMFSISAAGFTLMSLMCATSSSIGEMIVWRAL
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QGFIGGGMIPTVFAAAFTIFPPSKRSIVSPMIGLVATLAPTIGPTIGGYLTDLFSWHWLF
HHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LINIVPGIFVTISTFLLIDFDRPNFDLLKSFDWAGLAFMAGFLGCLEYVLEEGPNHDWLQ
HHHHHHHHHHHHHHHHEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCH
DEAVFVCALVCVVSGLAFFARVFTARQPIVDLRAFSDRNFAAGCVFSFVMGIGLYGLTYL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
YPVYLARVRGYSALQIGETMFVSGLCMFATAPIAGKLSAKLDPRIMMAMGFSGFAVGTWI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHH
VTGLTKDWDFWELLWPQVLRGCSLMLCMIPINNIALGTLPPERMKNASGLFNLTRNLGGA
HHCCCCCCHHHHHHHHHHHHHHHHEEHEEECCCEEECCCCHHHHCCHHHHHHHHHHHHHH
VGLALINTVLNARWDLHLARLHERFTWANSAALERLDAMRRQFEVFGGDANGMALKALNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
TVRIQGLVMSFEDVFLVLTVLFLAMACGTPLIRRPRAAAPAGAGH
CEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AASATLAASPAADPPLDRRRMVAFLCMVFGMFMAILDIQIVSASLNEIQAGLSASGDEI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC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CEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]