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The map label for this gene is yadF [C]

Identifier: 218532356

GI number: 218532356

Start: 4728865

End: 4729605

Strand: Reverse

Name: yadF [C]

Synonym: Mchl_4468

Alternate gene names: 218532356

Gene position: 4729605-4728865 (Counterclockwise)

Preceding gene: 218532357

Following gene: 218532351

Centisome position: 81.86

GC content: 68.42

Gene sequence:

>741_bases
ATGCACGCCACTTGCGATTGCTGCGCCGAGACACGTCCGCCGCATGCCGGCCGCCGACGTCTCCTGTTCGGGGCAGCGGG
CCTCCTCGCTGCGACCGCTCTGCCGAATGGTGTCGTGCGGGCTGCGACGCCCATGGCGAAGACGTCGCTGTCTCCGGCCG
ACGCGCTCCGGCTGATGAAGGAAGGCAACGAGAACTTCAGGAACGAGGCGCCTTCCCTAGCCGCCAACGGGCGCGAACGC
CGGCTCGAACTCGCCCGCGGCCAAGCCCCCTTCTGCGTTCTGGTGGGCTGCTCCGACAGCAGGGTCTCGCCGGAGATCCT
GTTCGGCCGCGGGCTCGGCGAGCTGTTCATCGTCCGGAACGCCGGCAACACGGTCGATACGGCGGCTTTGGGCAGCATCG
AGTATGCCGTCGGGGTCCTCGGCGTCCCCCTCGTCGTCGTGCTCGGCCATCAGTCCTGTGGCGCCGTCGCGGCCGCCGTC
GATGTCGTCGAGAAGAACGCAACCTTCCCCGGCGTCATCGGGGAGATGGTGCAGCCCATCGTGCCGGCGGTGCTGGAGGC
CAGGAGCCAGGGCGGCGACCTCCTCGAAGCGTCCGTGCGGAGCAACGCGCGCCGCGTCGCCAAACGGCTCACGACGCAGA
GCTTGGTGATTCAGGACGCGCTCAAGCAGGGCAAGGTGAAGGTCGTGGGTTCCCGCTACGGCCTCTCCGACGGTCAGGTC
GAGTGGATGGAAGATATCTGA

Upstream 100 bases:

>100_bases
GCCACGCCCGAAGAATAGATGAGGTGCATTGGCCGTCCGAAATGCCGTCGAAGCGGGCCGCGGACGGCGAAGCTCTTTCG
GAGCGAGGGGATCCGCCTTC

Downstream 100 bases:

>100_bases
GACGCCGTCCGCACCCGGCATCCCGGAGCGGTGGCCCGCACCGCGGGTATGAAGACGATCGTCTCTAGGCCGCCTTCACC
CGCGCGGCGCGATCCGGGAT

Product: carbonic anhydrase

Products: NA

Alternate protein names: Carbonate dehydratase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MHATCDCCAETRPPHAGRRRLLFGAAGLLAATALPNGVVRAATPMAKTSLSPADALRLMKEGNENFRNEAPSLAANGRER
RLELARGQAPFCVLVGCSDSRVSPEILFGRGLGELFIVRNAGNTVDTAALGSIEYAVGVLGVPLVVVLGHQSCGAVAAAV
DVVEKNATFPGVIGEMVQPIVPAVLEARSQGGDLLEASVRSNARRVAKRLTTQSLVIQDALKQGKVKVVGSRYGLSDGQV
EWMEDI

Sequences:

>Translated_246_residues
MHATCDCCAETRPPHAGRRRLLFGAAGLLAATALPNGVVRAATPMAKTSLSPADALRLMKEGNENFRNEAPSLAANGRER
RLELARGQAPFCVLVGCSDSRVSPEILFGRGLGELFIVRNAGNTVDTAALGSIEYAVGVLGVPLVVVLGHQSCGAVAAAV
DVVEKNATFPGVIGEMVQPIVPAVLEARSQGGDLLEASVRSNARRVAKRLTTQSLVIQDALKQGKVKVVGSRYGLSDGQV
EWMEDI
>Mature_246_residues
MHATCDCCAETRPPHAGRRRLLFGAAGLLAATALPNGVVRAATPMAKTSLSPADALRLMKEGNENFRNEAPSLAANGRER
RLELARGQAPFCVLVGCSDSRVSPEILFGRGLGELFIVRNAGNTVDTAALGSIEYAVGVLGVPLVVVLGHQSCGAVAAAV
DVVEKNATFPGVIGEMVQPIVPAVLEARSQGGDLLEASVRSNARRVAKRLTTQSLVIQDALKQGKVKVVGSRYGLSDGQV
EWMEDI

Specific function: Catalyzes the reversible hydration of carbon dioxide to form bicarbonate [H]

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786318, Length=165, Percent_Identity=35.7575757575758, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1786534, Length=167, Percent_Identity=31.7365269461078, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 25823; Mature: 25823

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1 ; PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHATCDCCAETRPPHAGRRRLLFGAAGLLAATALPNGVVRAATPMAKTSLSPADALRLMK
CCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHCCCHHHHHHHHH
EGNENFRNEAPSLAANGRERRLELARGQAPFCVLVGCSDSRVSPEILFGRGLGELFIVRN
CCCHHHHHCCCCHHCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEECCCCEEEEEEC
AGNTVDTAALGSIEYAVGVLGVPLVVVLGHQSCGAVAAAVDVVEKNATFPGVIGEMVQPI
CCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VPAVLEARSQGGDLLEASVRSNARRVAKRLTTQSLVIQDALKQGKVKVVGSRYGLSDGQV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCC
EWMEDI
HHHHCC
>Mature Secondary Structure
MHATCDCCAETRPPHAGRRRLLFGAAGLLAATALPNGVVRAATPMAKTSLSPADALRLMK
CCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHCCCHHHHHHHHH
EGNENFRNEAPSLAANGRERRLELARGQAPFCVLVGCSDSRVSPEILFGRGLGELFIVRN
CCCHHHHHCCCCHHCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEECCCCEEEEEEC
AGNTVDTAALGSIEYAVGVLGVPLVVVLGHQSCGAVAAAVDVVEKNATFPGVIGEMVQPI
CCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VPAVLEARSQGGDLLEASVRSNARRVAKRLTTQSLVIQDALKQGKVKVVGSRYGLSDGQV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCC
EWMEDI
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA