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The map label for this gene is emrA [H]

Identifier: 218532358

GI number: 218532358

Start: 4731703

End: 4732908

Strand: Reverse

Name: emrA [H]

Synonym: Mchl_4470

Alternate gene names: 218532358

Gene position: 4732908-4731703 (Counterclockwise)

Preceding gene: 218532360

Following gene: 218532357

Centisome position: 81.91

GC content: 74.21

Gene sequence:

>1206_bases
ATGTCGCTGCGTGAGGATTCCGGCCGGTCGGAGGCCGCCCCTGAGACGGCCGCGGAGGCGCGGGCCGAGTCCGGCCGGGC
CCCGGCCGGGCGCCCCGCCCCGGTTGCCGAGCCGGTGCCGCCCTCCCCCGCCAAGCCGCGCCGGCCGCTGCGGCGCGCGG
TGCTGGGCCTCGTGCTCTGCGCGGCGCTCGGCGGCGGCGCCTATGCCGGCTGGGACTGGTGGACGGTGGGGCGCTACTTC
GTCAGCACCGACGACGCCTACGTGCAGGCGGATATCTCGGTGCTCGCGGCCAAGGTGTCGGGCTATCTGGCGGCGGTGCC
CGTGGTGAACGGGCAGGCGGTGAAGCGGGGCGACGTGATCGCCCGGCTCGACGACGGCGATTACCGCCTCGCCCTGAAGG
CCGCCGCGGACAAGCTCGCCACGCAGGAGAGCACCATCGCCCGCGTCGGCCGGCAGGCCGAGGCGGCGCAGGCGCAGGTG
CTGCAGAGCGCAGCGCAGATCGACGCCGCGAAGGCCGACGCCGTGCGGGCCGGGGCCGACTACGCCCGCGCCACGGGGAT
GCAGGCCGATTTCGTCGCGAAATCCCGGCTTGATCAGGCCAAGGCCGATCGCGACCGCACCGAGGCGGCGGTGAAGTCGG
CCGAGGCCGCCCTCGTCGCCGCGCGGGCCAATGTCGAAGTGCTGAAGGCGCAGACACGCGAGGCCGAGAATCTCGCCGCG
GAGCTGCGCACCGCCGCCGACCGCGCGCGGCGCGACCTCGATTTCGCGGTGATCCGCGCGCCCTTCGACGGCGTCGTCGG
CAACAAGGCGGTGGAGGCCGGGGCCTATGTCTCGCCGGGCTCGCGCATCGCCGCCCTGGTGCCGCTGCAGAGCGTGCGGG
TCGATGCGAACTTCAAGGAGACACAGCTCGCCCGGGTCCGGCCCGGCCAGGAGGTCCATATCCACGTGGACGCCTATCCC
GACCGCGACATCGTCGGCACGGTCGAATCGCTCTCCCCCGCTTCCGGTTCGGTCTTCAGCCTGCTGCCACCGGACAACGC
CACCGGCAACTTCACCAAGATCGTGCAGCGCCTGCCCGTGCGCGTCCACGTGCCCGAGGAAGTCGCCCGCGAGGGCCTGC
TGCGGCCCGGCCTGTCCGTGGTGGTGAAGGTCGATACCCGCGAGGGCGCCGACGCACCGGTGCAGCGGCTCGCCGCCCGG
CCGTGA

Upstream 100 bases:

>100_bases
CCGTTCAGTCAGTGCTAGCTAGCCCTCGAATGAGGGCGGCGTCAACTCAGCCCGTCGAATGACCGAACGGTTCGGTCAAT
GCATGTCTGGGATGTCAGCG

Downstream 100 bases:

>100_bases
GACTCGAGCCGGTCAGCAAGAGCCGGCCGGCGCAACCGCTCGCCCCACCCACGCCCTCATCCTGAGGTGCCGGAGCGAAA
GCGGAGGCCTCGAAGGAGCC

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSLREDSGRSEAAPETAAEARAESGRAPAGRPAPVAEPVPPSPAKPRRPLRRAVLGLVLCAALGGGAYAGWDWWTVGRYF
VSTDDAYVQADISVLAAKVSGYLAAVPVVNGQAVKRGDVIARLDDGDYRLALKAAADKLATQESTIARVGRQAEAAQAQV
LQSAAQIDAAKADAVRAGADYARATGMQADFVAKSRLDQAKADRDRTEAAVKSAEAALVAARANVEVLKAQTREAENLAA
ELRTAADRARRDLDFAVIRAPFDGVVGNKAVEAGAYVSPGSRIAALVPLQSVRVDANFKETQLARVRPGQEVHIHVDAYP
DRDIVGTVESLSPASGSVFSLLPPDNATGNFTKIVQRLPVRVHVPEEVAREGLLRPGLSVVVKVDTREGADAPVQRLAAR
P

Sequences:

>Translated_401_residues
MSLREDSGRSEAAPETAAEARAESGRAPAGRPAPVAEPVPPSPAKPRRPLRRAVLGLVLCAALGGGAYAGWDWWTVGRYF
VSTDDAYVQADISVLAAKVSGYLAAVPVVNGQAVKRGDVIARLDDGDYRLALKAAADKLATQESTIARVGRQAEAAQAQV
LQSAAQIDAAKADAVRAGADYARATGMQADFVAKSRLDQAKADRDRTEAAVKSAEAALVAARANVEVLKAQTREAENLAA
ELRTAADRARRDLDFAVIRAPFDGVVGNKAVEAGAYVSPGSRIAALVPLQSVRVDANFKETQLARVRPGQEVHIHVDAYP
DRDIVGTVESLSPASGSVFSLLPPDNATGNFTKIVQRLPVRVHVPEEVAREGLLRPGLSVVVKVDTREGADAPVQRLAAR
P
>Mature_400_residues
SLREDSGRSEAAPETAAEARAESGRAPAGRPAPVAEPVPPSPAKPRRPLRRAVLGLVLCAALGGGAYAGWDWWTVGRYFV
STDDAYVQADISVLAAKVSGYLAAVPVVNGQAVKRGDVIARLDDGDYRLALKAAADKLATQESTIARVGRQAEAAQAQVL
QSAAQIDAAKADAVRAGADYARATGMQADFVAKSRLDQAKADRDRTEAAVKSAEAALVAARANVEVLKAQTREAENLAAE
LRTAADRARRDLDFAVIRAPFDGVVGNKAVEAGAYVSPGSRIAALVPLQSVRVDANFKETQLARVRPGQEVHIHVDAYPD
RDIVGTVESLSPASGSVFSLLPPDNATGNFTKIVQRLPVRVHVPEEVAREGLLRPGLSVVVKVDTREGADAPVQRLAARP

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=357, Percent_Identity=34.733893557423, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1788711, Length=365, Percent_Identity=32.3287671232877, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1790519, Length=300, Percent_Identity=30, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1789637, Length=362, Percent_Identity=25.9668508287293, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790024, Length=242, Percent_Identity=23.9669421487603, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789900, Length=367, Percent_Identity=25.3405994550409, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081951, Length=297, Percent_Identity=25.2525252525253, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790012, Length=244, Percent_Identity=26.6393442622951, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1787013, Length=311, Percent_Identity=26.6881028938907, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42262; Mature: 42131

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLREDSGRSEAAPETAAEARAESGRAPAGRPAPVAEPVPPSPAKPRRPLRRAVLGLVLC
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AALGGGAYAGWDWWTVGRYFVSTDDAYVQADISVLAAKVSGYLAAVPVVNGQAVKRGDVI
HHHCCCCCCCCCHHHHCEEEEECCCCEEHHHHHHHHHHHHCCEEEEEEECCCCEECCCEE
ARLDDGDYRLALKAAADKLATQESTIARVGRQAEAAQAQVLQSAAQIDAAKADAVRAGAD
EEECCCCEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
YARATGMQADFVAKSRLDQAKADRDRTEAAVKSAEAALVAARANVEVLKAQTREAENLAA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHH
ELRTAADRARRDLDFAVIRAPFDGVVGNKAVEAGAYVSPGSRIAALVPLQSVRVDANFKE
HHHHHHHHHHHCCCEEEEECCCCCCCCCCHHCCCCEECCCCCEEEEECHHHEEECCCCCH
TQLARVRPGQEVHIHVDAYPDRDIVGTVESLSPASGSVFSLLPPDNATGNFTKIVQRLPV
HHHHHCCCCCEEEEEEECCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCC
RVHVPEEVAREGLLRPGLSVVVKVDTREGADAPVQRLAARP
EEECCHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHCCCC
>Mature Secondary Structure 
SLREDSGRSEAAPETAAEARAESGRAPAGRPAPVAEPVPPSPAKPRRPLRRAVLGLVLC
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AALGGGAYAGWDWWTVGRYFVSTDDAYVQADISVLAAKVSGYLAAVPVVNGQAVKRGDVI
HHHCCCCCCCCCHHHHCEEEEECCCCEEHHHHHHHHHHHHCCEEEEEEECCCCEECCCEE
ARLDDGDYRLALKAAADKLATQESTIARVGRQAEAAQAQVLQSAAQIDAAKADAVRAGAD
EEECCCCEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
YARATGMQADFVAKSRLDQAKADRDRTEAAVKSAEAALVAARANVEVLKAQTREAENLAA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHH
ELRTAADRARRDLDFAVIRAPFDGVVGNKAVEAGAYVSPGSRIAALVPLQSVRVDANFKE
HHHHHHHHHHHCCCEEEEECCCCCCCCCCHHCCCCEECCCCCEEEEECHHHEEECCCCCH
TQLARVRPGQEVHIHVDAYPDRDIVGTVESLSPASGSVFSLLPPDNATGNFTKIVQRLPV
HHHHHCCCCCEEEEEEECCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCC
RVHVPEEVAREGLLRPGLSVVVKVDTREGADAPVQRLAARP
EEECCHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]