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The map label for this gene is purK [H]

Identifier: 218710993

GI number: 218710993

Start: 3257191

End: 3258321

Strand: Direct

Name: purK [H]

Synonym: VS_3121

Alternate gene names: 218710993

Gene position: 3257191-3258321 (Clockwise)

Preceding gene: 218710992

Following gene: 218710998

Centisome position: 98.72

GC content: 48.19

Gene sequence:

>1131_bases
ATGCATGTTCTTGTGTTAGGCGCGGGCCAACTTGCTCGCATGATGTCCCTAGCTGGGGCACCGCTGAATATTGAAATTTC
TGCTTTTGATGTTGGCAGCAAAAATATTGTTCACCCATTAACGCAAGCGATTCTAGGTAACGGCTTAGAAAATGCGATTG
AGCGTGCGGACGTCATTACTGCTGAATTCGAACATATTCCTCACGATGTGCTTGAGGTATGTGAGCGCAGCGGTAAGTTC
TTACCGACAACAGAAGCAATCAAAGCTGGCGGTGACCGTCGTCTTGAAAAAGCCCTGTTAGACGAAGCAAACGTGAAAAA
TGCTAAGTACTACGTGATTAACTCTCGCGAAGACTTTGACGCTGCGATTGCTCACGTAAGCTTACCAATGGTCTTGAAGA
GCACACTTGGCGGCTACGATGGCAAAGGCCAGTGGCGCTTAAAGACATTAGATAATGTTGAGTCAACTTGGACAGAAATG
GCTGAGTGCATTGCCGCAACAGACAACCAAGCGATTGTGGCTGAAGAGTTCGTTCCGTTTGACCGCGAAGTATCACTTGT
CGGTGCTCGTGGCGCCAATGGCGAGATCCAAGTGTACCCACTGGCTGAAAATGTTCACACCGACGGCGTGTTGAGCTTAT
CAACAGCCATTGATGACATTGAACTGCAAGAGCAAGCGAAAACCATGTTCACTGCCATTGCTGAGCGTTTAGATTACGTT
GGCGTGCTAGCACTTGAGTTCTTTGACGTTCAAGGTTCACTGCTTGTTAATGAGATTGCACCACGCGTTCATAACTCTGG
CCATTGGACACAGCAAGGCGCTGAAACTTGTCAGTTTGAGAACCACCTACGTGCTGTGTGTGGTATGCCACTAGGCAGTA
CTAAACTGATTCGCCCAACCGCAATGATCAACATTCTTGGTGAAGATACCCTACCTGGGGCTATTCTAGCTCAAGGTGGC
TGTCATGTTCATTGGTATGGCAAAGAGAAGCGTGCAGGTCGTAAGATGGGCCACATTAACGTGAGCGCTGACTACAACGC
AGAGCTGCAAAGAGCGTTATGCTCACTGGCAGACATTCTCGACAAACAAGCATATCCAGCAGTGCATGAGTTTGCTGAGC
AGATGAAGTAA

Upstream 100 bases:

>100_bases
GTACACACAATGAAGAAGTAATGGCAAAAGTAGAAGCGTTCCGCTCTGAACAAACAGAAACGGTTCTGGCTAATCCAAAC
CCTGCAGAGGACTAATTTCC

Downstream 100 bases:

>100_bases
ACCTACATTGGCTTATTGATATAAAAAGTTCATTGGATATAAAAATGGCGCTCAGTGAGCGCCGTTTTTTGTCTATAACA
TCAGGTTATTGAGATTGAAT

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MHVLVLGAGQLARMMSLAGAPLNIEISAFDVGSKNIVHPLTQAILGNGLENAIERADVITAEFEHIPHDVLEVCERSGKF
LPTTEAIKAGGDRRLEKALLDEANVKNAKYYVINSREDFDAAIAHVSLPMVLKSTLGGYDGKGQWRLKTLDNVESTWTEM
AECIAATDNQAIVAEEFVPFDREVSLVGARGANGEIQVYPLAENVHTDGVLSLSTAIDDIELQEQAKTMFTAIAERLDYV
GVLALEFFDVQGSLLVNEIAPRVHNSGHWTQQGAETCQFENHLRAVCGMPLGSTKLIRPTAMINILGEDTLPGAILAQGG
CHVHWYGKEKRAGRKMGHINVSADYNAELQRALCSLADILDKQAYPAVHEFAEQMK

Sequences:

>Translated_376_residues
MHVLVLGAGQLARMMSLAGAPLNIEISAFDVGSKNIVHPLTQAILGNGLENAIERADVITAEFEHIPHDVLEVCERSGKF
LPTTEAIKAGGDRRLEKALLDEANVKNAKYYVINSREDFDAAIAHVSLPMVLKSTLGGYDGKGQWRLKTLDNVESTWTEM
AECIAATDNQAIVAEEFVPFDREVSLVGARGANGEIQVYPLAENVHTDGVLSLSTAIDDIELQEQAKTMFTAIAERLDYV
GVLALEFFDVQGSLLVNEIAPRVHNSGHWTQQGAETCQFENHLRAVCGMPLGSTKLIRPTAMINILGEDTLPGAILAQGG
CHVHWYGKEKRAGRKMGHINVSADYNAELQRALCSLADILDKQAYPAVHEFAEQMK
>Mature_376_residues
MHVLVLGAGQLARMMSLAGAPLNIEISAFDVGSKNIVHPLTQAILGNGLENAIERADVITAEFEHIPHDVLEVCERSGKF
LPTTEAIKAGGDRRLEKALLDEANVKNAKYYVINSREDFDAAIAHVSLPMVLKSTLGGYDGKGQWRLKTLDNVESTWTEM
AECIAATDNQAIVAEEFVPFDREVSLVGARGANGEIQVYPLAENVHTDGVLSLSTAIDDIELQEQAKTMFTAIAERLDYV
GVLALEFFDVQGSLLVNEIAPRVHNSGHWTQQGAETCQFENHLRAVCGMPLGSTKLIRPTAMINILGEDTLPGAILAQGG
CHVHWYGKEKRAGRKMGHINVSADYNAELQRALCSLADILDKQAYPAVHEFAEQMK

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=365, Percent_Identity=36.7123287671233, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1788155, Length=322, Percent_Identity=23.9130434782609, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6324702, Length=387, Percent_Identity=30.4909560723514, Blast_Score=154, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 41013; Mature: 41013

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVLVLGAGQLARMMSLAGAPLNIEISAFDVGSKNIVHPLTQAILGNGLENAIERADVIT
CEEEEECCHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
AEFEHIPHDVLEVCERSGKFLPTTEAIKAGGDRRLEKALLDEANVKNAKYYVINSREDFD
HHHHHCCHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEECCCCHH
AAIAHVSLPMVLKSTLGGYDGKGQWRLKTLDNVESTWTEMAECIAATDNQAIVAEEFVPF
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCC
DREVSLVGARGANGEIQVYPLAENVHTDGVLSLSTAIDDIELQEQAKTMFTAIAERLDYV
CCCEEEEECCCCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVLALEFFDVQGSLLVNEIAPRVHNSGHWTQQGAETCQFENHLRAVCGMPLGSTKLIRPT
HEEEHHEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECHH
AMINILGEDTLPGAILAQGGCHVHWYGKEKRAGRKMGHINVSADYNAELQRALCSLADIL
HEEEEECCCCCCCHHEECCCCEEEEECCHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHH
DKQAYPAVHEFAEQMK
HHCCCHHHHHHHHHCC
>Mature Secondary Structure
MHVLVLGAGQLARMMSLAGAPLNIEISAFDVGSKNIVHPLTQAILGNGLENAIERADVIT
CEEEEECCHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
AEFEHIPHDVLEVCERSGKFLPTTEAIKAGGDRRLEKALLDEANVKNAKYYVINSREDFD
HHHHHCCHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEECCCCHH
AAIAHVSLPMVLKSTLGGYDGKGQWRLKTLDNVESTWTEMAECIAATDNQAIVAEEFVPF
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCC
DREVSLVGARGANGEIQVYPLAENVHTDGVLSLSTAIDDIELQEQAKTMFTAIAERLDYV
CCCEEEEECCCCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVLALEFFDVQGSLLVNEIAPRVHNSGHWTQQGAETCQFENHLRAVCGMPLGSTKLIRPT
HEEEHHEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECHH
AMINILGEDTLPGAILAQGGCHVHWYGKEKRAGRKMGHINVSADYNAELQRALCSLADIL
HEEEEECCCCCCCHHEECCCCEEEEECCHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHH
DKQAYPAVHEFAEQMK
HHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]