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The map label for this gene is def [H]

Identifier: 218710998

GI number: 218710998

Start: 3261701

End: 3262321

Strand: Direct

Name: def [H]

Synonym: VS_3126

Alternate gene names: 218710998

Gene position: 3261701-3262321 (Clockwise)

Preceding gene: 218710993

Following gene: 218710999

Centisome position: 98.86

GC content: 43.48

Gene sequence:

>621_bases
CTGATTAGAGAATTACCTTCAGGATGCTGTCATTTGACCTCTAAAATGTCTAGAATTGAGCCAACAAGGTTTAAGCTGTT
TCGGCACAGTTCAATATTTCGAGTGTATATGTCTGTATTACAAGTATTAACATTACCAGATAATCGTCTACGTACCGTGG
CGAAACCGGTAAAAGAAGTTACCCCAGAGATTCAAAAGTTCGTTGATGACATGATTGAAACCATGTACGACGAAGAAGGT
ATCGGCCTTGCGGCAACGCAAGTAGATTTCCACCAACGCATCGTTGTTATCGATATTTCAGAAACACGTGACGAGCCTAT
GGTTCTGATCAACCCTGAAATTACCGACAAACGTGGCGAAGATGGTATCGAAGAAGGCTGTCTATCTGTACCAGGCGCTC
GAGCTCTAGTACCTCGCGCTGCAGAAGTAACGGTTAAAGCTTTAGACCGTGAAGGCAACGAATTCACATTCGACGCTGAC
GACCTTCTGGCTATCTGTGTTCAGCACGAACTTGACCACCTAGAAGGCAAGTTGTTTGTTGATTACCTATCGCCACTAAA
GCGCAAACGTATTCAAGATAAGCTAGCGAAGATTAAACGTTTCAACGAGAAACAAGGTTAA

Upstream 100 bases:

>100_bases
TTTTCAGCCATAACGGCGAACGAAAGCGAGGCACAAATAAGAGATAAAGCGGGGGAAAAATGACGCATGACCTAGGTTCC
TTGGTCTGAATATATGACAT

Downstream 100 bases:

>100_bases
TAACCGCTGCTATTACTAAATTAAGAAGGAAGTCTACCTTGAGTCAATCTTTAAGAATTGTCTTCGCAGGTACTCCGGAT
TTCGCCGCCCGTCATTTGGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MIRELPSGCCHLTSKMSRIEPTRFKLFRHSSIFRVYMSVLQVLTLPDNRLRTVAKPVKEVTPEIQKFVDDMIETMYDEEG
IGLAATQVDFHQRIVVIDISETRDEPMVLINPEITDKRGEDGIEEGCLSVPGARALVPRAAEVTVKALDREGNEFTFDAD
DLLAICVQHELDHLEGKLFVDYLSPLKRKRIQDKLAKIKRFNEKQG

Sequences:

>Translated_206_residues
MIRELPSGCCHLTSKMSRIEPTRFKLFRHSSIFRVYMSVLQVLTLPDNRLRTVAKPVKEVTPEIQKFVDDMIETMYDEEG
IGLAATQVDFHQRIVVIDISETRDEPMVLINPEITDKRGEDGIEEGCLSVPGARALVPRAAEVTVKALDREGNEFTFDAD
DLLAICVQHELDHLEGKLFVDYLSPLKRKRIQDKLAKIKRFNEKQG
>Mature_206_residues
MIRELPSGCCHLTSKMSRIEPTRFKLFRHSSIFRVYMSVLQVLTLPDNRLRTVAKPVKEVTPEIQKFVDDMIETMYDEEG
IGLAATQVDFHQRIVVIDISETRDEPMVLINPEITDKRGEDGIEEGCLSVPGARALVPRAAEVTVKALDREGNEFTFDAD
DLLAICVQHELDHLEGKLFVDYLSPLKRKRIQDKLAKIKRFNEKQG

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=158, Percent_Identity=31.0126582278481, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1789682, Length=168, Percent_Identity=68.4523809523809, Blast_Score=241, Evalue=3e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 23525; Mature: 23525

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRELPSGCCHLTSKMSRIEPTRFKLFRHSSIFRVYMSVLQVLTLPDNRLRTVAKPVKEV
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TPEIQKFVDDMIETMYDEEGIGLAATQVDFHQRIVVIDISETRDEPMVLINPEITDKRGE
CHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCEEEEEEECCCCCCCEEEECCCCCCCCCC
DGIEEGCLSVPGARALVPRAAEVTVKALDREGNEFTFDADDLLAICVQHELDHLEGKLFV
CCHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCCHHHH
DYLSPLKRKRIQDKLAKIKRFNEKQG
HHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIRELPSGCCHLTSKMSRIEPTRFKLFRHSSIFRVYMSVLQVLTLPDNRLRTVAKPVKEV
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TPEIQKFVDDMIETMYDEEGIGLAATQVDFHQRIVVIDISETRDEPMVLINPEITDKRGE
CHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCEEEEEEECCCCCCCEEEECCCCCCCCCC
DGIEEGCLSVPGARALVPRAAEVTVKALDREGNEFTFDADDLLAICVQHELDHLEGKLFV
CCHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCCHHHH
DYLSPLKRKRIQDKLAKIKRFNEKQG
HHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA