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The map label for this gene is gppA

Identifier: 218710960

GI number: 218710960

Start: 3217892

End: 3219385

Strand: Direct

Name: gppA

Synonym: VS_3074

Alternate gene names: 218710960

Gene position: 3217892-3219385 (Clockwise)

Preceding gene: 218710959

Following gene: 218710964

Centisome position: 97.53

GC content: 47.72

Gene sequence:

>1494_bases
ATGAGTCAAACAGTGTCACCACCGCTTTATGCTGCAATCGACCTCGGGTCGAACAGTTTTCATATGCTCGTTGTGCGTCA
TATCGATGGCAGCGTACAAACCATGGCTAAAATTAAGCGCAAAGTTCGTTTAGCTGCAGGCTTAGATGAAAATAATGCGC
TTAGTACTGAAGCCATGCAGCGCGGTTGGGACTGTTTGAGTCTCTTTGCAGAGCGACTGCGAGATATTCCGAAAGAAAAT
ATCCGCATTGTTGGTACAGCGACCCTACGTACCGCTATCAATGTGGATATATTTCTCGAGAAAGCGAACCAGATCCTTGG
TTACGACATCAATGTTATCTCTGGTGAAGAAGAAGCTGCGACTATCTATAAAGGCGTAGCACACACTTCTGGTGGCAGCG
GCCGCCGACTAGTGGTTGATATTGGTGGTGCAAGTACCGAGATGATCATCGGTGAAGGTTTCTCAGCCAAAGCCTTAACC
AGCCTGAAGATGGGTTGTGTTACTTGGCTTGAACGCCACTTTAAAGATCGCCAATTAACCGCAACCAACTTCAACAATGC
CATTGAAGCGGCCAAGTCTACGTTGGCTCCTATCCTAGATAGCTACACTGATATCGGGTGGGATGTGTGTGTTGGTGCCA
GTGGTACCGTTCAAGCACTGCAAGAAATCATGTTGGCGCAAGGCATGGATGAAGTCATTACTCATGCCAAACTCAAGCGT
CTACAGAAACAAGCGATGATTACAGAGCGCTTAGAAGAGCTTGAGATTGAAGGCTTAACCCTTGAGCGCGCACTAGTGTT
CCCAAGTGGTCTTTCTATTCTTATCGCGATTTTTGAGCTGCTTGAAATTGATTCAATGACACTCGCTGGCGGCGCACTCC
GTGAAGGTCTCGCCTACGAGATGGTTGATGAGTTTCGCCAAGAAGATATTCGCGCTCGTACCATTAAGAGCGTGCAATCA
CGCTACCAAATGGACGTAAGCTACAGTGAACAAGTTGCTGTAGTCGCACAGACTCTATTAGAGCAAGCGGGCGCCGAAAC
GTGGGTTTCTGAACCTCAAGCAGGTGTGCTATTACAAACAGCGGCGAAGCTTCATGAGATTGGTTTAACAATCGATTTCA
AAAAAGGCGGCGAGCACAGTGCTTACCTACTGCAGAACTTAGACCTACCAGGCTTTACTCGAGCGCAAAAGCACTGCTTG
GGTGAGTTAACACGTCGCTACCGCGAACAGTTAACTTCATTGCCAGAGCAACATGCAATCTCAGGTACCAGCAGCAAGCG
TATTTTACGAATTCTGCGTTTAGCTATTTTGTTGACTCACCGTCGTAATCCAGCGCTTGAGCCTGAGTTCAAGCTGACGA
CGGATGACAACAATCTGACCTTGACGCTTTCTAAGCAATGGCTAGCAGACAACCCGCTGACCGCTGCTGAGCTAGAGATC
GAGTCAAATAGACAAACCGATATTGGCTGGCCATTGAATATTGAATGCCTCTGA

Upstream 100 bases:

>100_bases
ACGCCGCTCAAACAACAATGGCCCACGCAACGGTAACCGTAAACCAAACCAGAATCGTCGCCCACGCCAACCGCGTCATA
ACAAGGAAGCTTAGTCGCTT

Downstream 100 bases:

>100_bases
TATTAGTATCTGACGCTAGAAATTAAAAAAGCCCAAGTTAGTCTACTAACTTGGGCTTTTTCTTATCGCTGCATTAATGC
TTTGCGTTACAAATCTAAAC

Product: guanosine pentaphosphate phosphohydrolase

Products: NA

Alternate protein names: Guanosine pentaphosphate phosphohydrolase; pppGpp-5'-phosphohydrolase

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MSQTVSPPLYAAIDLGSNSFHMLVVRHIDGSVQTMAKIKRKVRLAAGLDENNALSTEAMQRGWDCLSLFAERLRDIPKEN
IRIVGTATLRTAINVDIFLEKANQILGYDINVISGEEEAATIYKGVAHTSGGSGRRLVVDIGGASTEMIIGEGFSAKALT
SLKMGCVTWLERHFKDRQLTATNFNNAIEAAKSTLAPILDSYTDIGWDVCVGASGTVQALQEIMLAQGMDEVITHAKLKR
LQKQAMITERLEELEIEGLTLERALVFPSGLSILIAIFELLEIDSMTLAGGALREGLAYEMVDEFRQEDIRARTIKSVQS
RYQMDVSYSEQVAVVAQTLLEQAGAETWVSEPQAGVLLQTAAKLHEIGLTIDFKKGGEHSAYLLQNLDLPGFTRAQKHCL
GELTRRYREQLTSLPEQHAISGTSSKRILRILRLAILLTHRRNPALEPEFKLTTDDNNLTLTLSKQWLADNPLTAAELEI
ESNRQTDIGWPLNIECL

Sequences:

>Translated_497_residues
MSQTVSPPLYAAIDLGSNSFHMLVVRHIDGSVQTMAKIKRKVRLAAGLDENNALSTEAMQRGWDCLSLFAERLRDIPKEN
IRIVGTATLRTAINVDIFLEKANQILGYDINVISGEEEAATIYKGVAHTSGGSGRRLVVDIGGASTEMIIGEGFSAKALT
SLKMGCVTWLERHFKDRQLTATNFNNAIEAAKSTLAPILDSYTDIGWDVCVGASGTVQALQEIMLAQGMDEVITHAKLKR
LQKQAMITERLEELEIEGLTLERALVFPSGLSILIAIFELLEIDSMTLAGGALREGLAYEMVDEFRQEDIRARTIKSVQS
RYQMDVSYSEQVAVVAQTLLEQAGAETWVSEPQAGVLLQTAAKLHEIGLTIDFKKGGEHSAYLLQNLDLPGFTRAQKHCL
GELTRRYREQLTSLPEQHAISGTSSKRILRILRLAILLTHRRNPALEPEFKLTTDDNNLTLTLSKQWLADNPLTAAELEI
ESNRQTDIGWPLNIECL
>Mature_496_residues
SQTVSPPLYAAIDLGSNSFHMLVVRHIDGSVQTMAKIKRKVRLAAGLDENNALSTEAMQRGWDCLSLFAERLRDIPKENI
RIVGTATLRTAINVDIFLEKANQILGYDINVISGEEEAATIYKGVAHTSGGSGRRLVVDIGGASTEMIIGEGFSAKALTS
LKMGCVTWLERHFKDRQLTATNFNNAIEAAKSTLAPILDSYTDIGWDVCVGASGTVQALQEIMLAQGMDEVITHAKLKRL
QKQAMITERLEELEIEGLTLERALVFPSGLSILIAIFELLEIDSMTLAGGALREGLAYEMVDEFRQEDIRARTIKSVQSR
YQMDVSYSEQVAVVAQTLLEQAGAETWVSEPQAGVLLQTAAKLHEIGLTIDFKKGGEHSAYLLQNLDLPGFTRAQKHCLG
ELTRRYREQLTSLPEQHAISGTSSKRILRILRLAILLTHRRNPALEPEFKLTTDDNNLTLTLSKQWLADNPLTAAELEIE
SNRQTDIGWPLNIECL

Specific function: Conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the "stringent response", an adaptive process that allows bacteria to respond to amino acid starvation, resulting in th

COG id: COG0248

COG function: function code FP; Exopolyphosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gppA/ppx family. GppA subfamily

Homologues:

Organism=Escherichia coli, GI48994966, Length=488, Percent_Identity=56.3524590163934, Blast_Score=534, Evalue=1e-153,
Organism=Escherichia coli, GI1788848, Length=504, Percent_Identity=40.2777777777778, Blast_Score=355, Evalue=4e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPPA_VIBSL (B7VMF0)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002418581.1
- ProteinModelPortal:   B7VMF0
- GeneID:   7162527
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_3074
- HOGENOM:   HBG451789
- OMA:   AMQRGWQ
- ProtClustDB:   PRK11031
- HAMAP:   MF_01550
- InterPro:   IPR003695

Pfam domain/function: PF02541 Ppx-GppA

EC number: =3.6.1.40

Molecular weight: Translated: 54929; Mature: 54798

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTVSPPLYAAIDLGSNSFHMLVVRHIDGSVQTMAKIKRKVRLAAGLDENNALSTEAMQ
CCCCCCCCCEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
RGWDCLSLFAERLRDIPKENIRIVGTATLRTAINVDIFLEKANQILGYDINVISGEEEAA
CCHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHEEEEECCCHHCCCEEEEECCCHHHH
TIYKGVAHTSGGSGRRLVVDIGGASTEMIIGEGFSAKALTSLKMGCVTWLERHFKDRQLT
HHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEE
ATNFNNAIEAAKSTLAPILDSYTDIGWDVCVGASGTVQALQEIMLAQGMDEVITHAKLKR
ECCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHH
LQKQAMITERLEELEIEGLTLERALVFPSGLSILIAIFELLEIDSMTLAGGALREGLAYE
HHHHHHHHHHHHHHCCCCEEEEHEEECCCHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHH
MVDEFRQEDIRARTIKSVQSRYQMDVSYSEQVAVVAQTLLEQAGAETWVSEPQAGVLLQT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHH
AAKLHEIGLTIDFKKGGEHSAYLLQNLDLPGFTRAQKHCLGELTRRYREQLTSLPEQHAI
HHHHHHHCCEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCC
SGTSSKRILRILRLAILLTHRRNPALEPEFKLTTDDNNLTLTLSKQWLADNPLTAAELEI
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEEHHHCCCCCCCEEEEEE
ESNRQTDIGWPLNIECL
CCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
SQTVSPPLYAAIDLGSNSFHMLVVRHIDGSVQTMAKIKRKVRLAAGLDENNALSTEAMQ
CCCCCCCCEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
RGWDCLSLFAERLRDIPKENIRIVGTATLRTAINVDIFLEKANQILGYDINVISGEEEAA
CCHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHEEEEECCCHHCCCEEEEECCCHHHH
TIYKGVAHTSGGSGRRLVVDIGGASTEMIIGEGFSAKALTSLKMGCVTWLERHFKDRQLT
HHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEE
ATNFNNAIEAAKSTLAPILDSYTDIGWDVCVGASGTVQALQEIMLAQGMDEVITHAKLKR
ECCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHH
LQKQAMITERLEELEIEGLTLERALVFPSGLSILIAIFELLEIDSMTLAGGALREGLAYE
HHHHHHHHHHHHHHCCCCEEEEHEEECCCHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHH
MVDEFRQEDIRARTIKSVQSRYQMDVSYSEQVAVVAQTLLEQAGAETWVSEPQAGVLLQT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHH
AAKLHEIGLTIDFKKGGEHSAYLLQNLDLPGFTRAQKHCLGELTRRYREQLTSLPEQHAI
HHHHHHHCCEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCC
SGTSSKRILRILRLAILLTHRRNPALEPEFKLTTDDNNLTLTLSKQWLADNPLTAAELEI
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEEHHHCCCCCCCEEEEEE
ESNRQTDIGWPLNIECL
CCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA